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Biomedical subjects

R Müller

Publications and source records attributed to R Müller.

At least 757 records · Page 42Linked to original sources
PubMed1980

Analysis of T-lymphocyte subpopulations in juvenile-onset diabetics.

In juvenile-onset diabetics (JOB) subpopulations of T-lymphocytes in the peripheral blood have been analysed by the E-rosetting technique. We determined the number of 'low', 'intermediate' and 'high affinity' ('active') rosette forming T-cells by the use of three different lymphocyte:sheep red blood cell ratios in the assay. When comparing the percentage and absolute number of peripheral T-cells between twenty well controlled JODs and twenty normal persons a slight reduction was found in diabetics. Of the three T-lymphocyte subpopulations only active rosette forming cells appeared to be affected. We conclude that minor differences exist in number and characteristics of peripheral blood T-cells between well controlled JODs and normal persons.

Adolescent↗
PubMed1980

Haemorheological role of platelet aggregation and hypercoagulability in microcirculation: therapeutical approach with pentoxifylline.

The pathophysiology of disturbances in platelet function and intravasal hypercoagulability in diseases with impaired microcirculation is reviewed, and related relevant experimental and clinical findings with pentoxifylline are presented. Recent studies have revealed the antithrombotic properties of pentoxifylline which contribute to its haemorheological profile and therapeutical efficacy.

Blood Coagulation↗
PubMed1980

[Hyperprolactinaemic endocrinopathy in gynaecological practice (author's transl)].

Prolactin and, to some extent, the gonadotrophins were determined in the serum of 50 patients with amenorrhoea (oligomenorrhoea) and or galactorrhoea. -- Hyperprolactinaemia was safely recorded from 32 cases. That relatively high number of patients with hyperprolactinaemia was attributed to retrograde examination of sterility patients who had been resistant to therapy. -- In the presence of galactorrhoea prolactin levels were increased in nine of 16 patients, whereas the same levels were increased in only three of 18 patients with postpill amenorrhoea. -- Gonadotrophin levels usually dropped in concomitance with marked hyperprolactinaemia, but they failed to deviate from normal with moderate prolactin increase. -- The above results are likely to underscore the importance of prolactin to the diagnosis of gynaecological endocrinopathies.

Adolescent↗
PubMed1980

[Anovulation syndrome in nulligravidae following intake of hormonal contraceptives (author's transl)].

Evaluated in this paper is clinical experience recorded from 70 young women, aged between 14 and 27 years, with post-pill syndrome. The aetiology of post-pill amenorrhoea was found to be closely related to lability of cycle prior to pill-taking. Nearly 60 per cent of all patients had used Non-Ovlon. 10 patients had used Sequenz-Ovosiston. The prognosis of iatrogenic normogonadotrophic ovarian insufficiency was found to depend on the basal oestrogen level, reaction of corpus endometrium, and serum prolactin. Pregnancy was successfully induced in 53 per cent of all patients who wanted to have a child. The aboration rate accounted for 7.4 per cent. Clinical examination of the newborns did not give any clue as to accumulation of congenital malformations. Roughly 40 per cent of all patients remained to be anovulatory, in spite of intensive treatment.

Adolescent↗
PubMed1980

Immunological quantification by high-affinity antibodies of O6-ethyldeoxyguanosine in DNA exposed to N-ethyl-N-nitrosourea.

Three immunological methods [radioimmunoassay (RIA), enzyme-linked immunosorbent assay, and radioimmunosorbent technique] were established for quantification of the potentially mutagenic O6-ethyldeoxyguanosine (O6-EtdGuo) in DNA treated with the carcinogen ethylnitrosourea in vivo or in vitro. To obtain high-affinity antibodies for specific detection of low levels of O6-EtdGuo in small amounts of DNA (cells), different schemes were applied for immunization of rabbits with the hapten O6-ethylguanosine coupled to various carrier proteins (rat serum albumin, bovine serum albumin, keyhold limpet hemocyanin). Low-dose immunization with the hapten-keyhold limpet hemocyanin conjugate resulted in antibodies with an affinity constant of 1 to 2 X 10(10) liters/mol and very low levels of cross-reactivity with normal as well as other alkylated DNA components. The RIA (the most sensitive of the three assays) detects 0.05 pmol of O6-EtdGuo at 50% inhibition of tracer (O6-ethyl[8,5'-3H]-3'-deoxyguanosine)-antibody binding. This permits quantification by RIA of O6-EtdGuo at an O6-EtdGuo:2'-deoxyguanosine molar ratio of approximately 3 X 10(-7) in a hydrolysate of 100 micrograms of ethylated DNA. By chromatographic separation of O6-EtdGuo prior to the RIA, this value can be lowered to less than 5 X 10(-8).

Animals↗
PubMed1979

[Epidemiology and prognosis of non-A, non-B hepatitis (author's transl)].

The frequency of non-A, non-B hepatitis (n = 325) was determined among all cases (n = 1368) of acute viral hepatitis observed in the Hannover are abetwen 1975 and 1978. Hepatitis A was excluded by demonstration of anti-HAV-IgM, hepatitis B by demonstration of HBs antigen or an isolated occurrence of anti-HBc at the beginning of the disease. Non-A, non-B hepatitis occurred predominantly in adults and showed no seasonal variability. As a consequence of results of followup investigations in 174 hepatitis patients 2 years after the onset of the disease it can be assumed that non-A, non-B hepatitis tends to lead to chronic courses more frequently than hepatitis B.

Adolescent↗
PubMed1979

Studies on the binding of the ribosomal protein complex L7/12-L10 and protein L11 to the 5'-one third of 23S RNA: a functional centre of the 50S subunit.

The RNA binding sites of the protein complex of L7/12 dimers and L10, and of protein L11, occur within the 5'-one third of 23S RNA. Binding of the L7/12-L10 protein complex to the 23S RNA is stimulated by protein L11 and vice-versa. This is the second example to be established of mutual stimulation of RNA binding by two ribosomal proteins or protein complexes, and suggests that this may be an important principle governing ribosomal protein-RNA assembly. When the L7/12-L10 complex is bound to the RNA, L10 becomes strongly resistant to trypsin. Since the L7/12 dimer does not bind specifically to the 23S RNA, this suggests that L10 constitutes a major RNA binding site of the protein complex. Only one of the L7/12 dimers is bound strongly in the (L7/12-L10)-23S RNA complex; the other can dissociate with no concurrent loss of L10.

Escherichia coli↗
PubMed1979

The structure of the RNA binding site of ribosomal proteins S8 and S15.

Proteins S8 and S15 from the 30 S ribosomal subunit of Escherichia coli were bound to 16 S RNA and digested with ribonuclease A. A ribonucleoprotein complex was isolated which contained the two proteins and three noncontiguous RNA subfragments totaling 93 nucleotides, that could be unambiguously located in the 16 S RNA sequence. We present a secondary structural model for the RNA moiety of the binding site complex, in which the two smaller fragments are extensively base-paired, respectively, to the two halves of the large fragment, to form two disconnected duplexes. Each of the two duplexes is interrupted by a small internal loop. This model is supported by (i) minimum energy considerations, (ii) sites of cleavage by ribonuclease A, and (iii) modification by the single strand-specific reagent kethoxal. The effect of protein binding on the topography of the complex is reflected in the kethoxal reactivity of the RNA moiety. In the absence of the proteins, 5 guanines are modified; 4 of these, at positions 663, 732, 733, and 741, are strongly protected from kethoxal when protein S15 is bound.

Base Sequence↗
PubMed1979

Effect of polyenyl phosphatidyl choline on clofibrate-induced increase in LDL cholesterol.

In a double-blind, randomised, cross-over trial clofibrate and a combination of polyenyl phosphatidyl choline (PPC) plus clofibrate were tested in 67 patients with hyperlipoproteinemia. Each treatment lasted for 4 weeks and was separated by a 4 week placebo period. The daily doses were clofibrate 1.2 g and PPC 1.8 g + clofibrate 1.2 g. respectively. The results revealed that polypenyl phosphatidyl choline prevented the elevation of LDL-cholesterol induced by clofibrate treatment, and that the lipid-lowering potency of the combination did not differ significantly from that of clofibrate. Since elevation of LDL-cholesterol is considered to increase the risk of coronary heart disease, the combination appears to offer a therapeutic advantage. Despite the significance of this clinical observation, a final decision may only be obtained from a prospective, long term investigation in patients with coronary heart diseases and hyperlipoproteinemia.

Adult↗