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Biomedical subjects

R M Coleman

Publications and source records attributed to R M Coleman.

49 records · Page 3Linked to original sources
PubMed1975

Splenic mediated erythrocyte cytotoxicity in malaria.

Cell-mediated cytotoxicity in virulent rodent malaria has been demonstrated in vitro, whereby splenic cells effected specific lysis of 51Cr-labelled erythrocytes from parasitized animals. More than one cellular cytolytic effector system appeared to be operative in the mouse. One effector system involved splenic macrophages, from normal or immune animals, which were increasingly cytotoxic to target cells in the presence of antibody. A second effector system involved nylonpurified immune spleen cells which were significantly more cytotoxic than similary prepared normal spleen cells in the presence of immune serum. Although antibody alone was not cytolytic, the data strengthen the concept that immune spleen cells and antibody can interact in a co-operative fashion to mediate cytotoxic reactions in malaria.

Animals↗
PubMed1974

Erythropoietin production in virulent malaria.

Erythropoietin, the hormone responsible for stimulating erythrocyte production, was shown to increase significantly in the serum of mice during virulent malaria infection. Although erythropoiesis was enhanced, it did not keep pace with the rate of erythocyte destruction; hence all Plasmodium berghei-infected mice quickly succumbed to the deleterious consequences of severe uncompensated hemolytic anemia. Since this apparently inadequate rate of erythropoiesis is not attributed to impaired erythropoietin generation, mechanisms relating to (i) hemopoietic stem-cell resistance to endogenous erythropoietin, (ii) deficits in numbers of hemopoietic stem cells, and/or (iii) ineffective erythropoiesis are of interest.

Anemia, Hemolytic↗
PubMed1972

Use of the immunodiffusion test in the serodiagnosis of aspergillosis.

The diagnostic value of an immunodiffusion (ID) test with standardized precipitinogens derived from five Aspergillus species was determined with sera from 60 proven and 12 suspected cases of aspergillosis. The data demonstrated that the greatest number of aspergillosis cases were detected by the concurrent use of A. fumigatus and A. niger precipitinogens. With these precipitinogens, the ID test permitted the serodiagnosis of aspergillosis in 82% of the 60 proven cases and in 83% of the 12 suspected cases. The presence of one or more precipitins was indicative of aspergilloma, of allergic bronchopulmonary aspergillosis, or of invasive aspergillosis. Precipitins were detected in 93% of the sera from patients with aspergilloma, in 50% of the sera from patients with allergic bronchopulmonary aspergillosis, and in 88% of the sera from patients with invasive aspergillosis. Although the presence of one or two precipitin bands could indicate any form of aspergillosis, the presence of three or four was strong evidence of either aspergilloma or invasive aspergillosis. The ID test was found to be 100% specific in an evaluation of its effectiveness with 65 sera from individuals with other systemic mycotic infections, bacterial or neoplastic diseases, and from apparently normal humans. In diagnosed cases of aspergillosis, the examination of serial serum specimens provided information about the clinical course of the disease. A reduction in the number of precipitin bands and significant titer changes were noted as the patients responded to therapy.

Animals↗
PubMed1969

Actinomyces naeslundii as an agent of human actinomycosis.

The repeated isolation of Actinomyces naeslundii from clinical materials associated with disease led to a comparison of isolates from the normal mouth with isolates from pathological clinical materials not from the mouth area. No important differences were observed between the isolates from these two sources. A human case of empyema of the gall bladder, apparently due to A. naeslundii, is described.

Actinomyces↗
PubMed1969

Comparative pathogenicity of Actinomyces naeslundii and Actinomyces israelii.

Typical actinomycosis has been produced in mice following single intraperitoneal injections of saline suspensions of Actinomyces israelii and A. naeslundii. A. israelii produced infections in 95.8% of the animals inoculated. A. naeslundii, generally considered to be a saprophytic organism, produced lesions in 89.7% of the inoculated animals. The finding that A. naeslundii produced lesions in mice similar to those produced by A. israelii suggests that A. naeslundii has similar pathogenic potential for man. The isolation of A. naeslundii from suppurative lesions of man also supports this conclusion.

Actinomyces↗
PubMedDate not supplied

Acquisition of concomitant oral and genital infection with herpes simplex virus type 2.

Primary oral and genital infections caused by herpes simplex virus type 2 were diagnosed in an 18-year-old female. A history of sexual practices was critical in determining the anatomic sites of infection. Restriction endonuclease analysis of viral DNAs helped to identify the male sexual partner from whom the virus had been acquired. He had been infected recently by a previous sexual partner but had not yet developed lesions. Clinicians should obtain a history of sexual practices from patients with newly acquired genital herpes and should advise patients with genital herpes that transmission of virus to sexual partners can occur in the absence of overt lesions.

Adolescent↗
PubMedDate not supplied

Transplantable Marek's disease lymphomas. I. Growth characteristics during development in two inbred lines of chickens.

Lymphomas developed in the pectoral muscle of most chickens inoculated with cells from primary Marek's disease virus-induced visceral tumors obtained from chickens of the same inbred line. However, serial passaging of the lymphoma cells in histocompatible hosts generally resulted either in an eventual absence of tumor formation at the inoculation site or in tumor regression. Exceptions occurred in two experiments, where tumors grew rapidly and the hosts died early. Subsequent passaging of cells from these tumors into syngeneic recipients resulted in the development of two new transplantable Marek's disease (MD) lymphomas. These lymphomas, which were developed in chickens of related inbred lines--G-B1 and G-B2--were designated MDCT-UG1 and MDCT-UG2, respectively. Cells from the transplantable lymphomas possess different major histocompatibility complex (MHC) antigens, since G-B1 and G-B2 chickens have different MHC genotypes. A change in the cellular composition during a particular passage for both lymphomas, as indicated by marked increases in the percentage of cells possessing a MD tumor-associated surface antigen (MATSA), suggests that each arose as a result of the emergence and selection of a highly malignant clone of cells.

Animals↗