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Biomedical subjects

R Kohler

Publications and source records attributed to R Kohler.

At least 73 records · Page 4Linked to original sources
PubMed1983

Immunocytochemical investigations of murine leukodystrophies. A study of the mutants 'jimpy' (jp) and 'myelin deficient' (mld).

Sections of the central nervous system of the leukodystrophic mouse mutants 'jimpy' (jp) and 'myelin deficient' (mld), as well as of healthy littermates, were immunostained for glial fibrillary acidic protein (GFA), myelin basic protein (MBP) and myelin-associated glycoprotein (MAG). Adjacent sections were stained conventionally for myelin. In jp, GFA-stained astrocytes were abnormally prominent already at the age of 12 days. A considerable amount of MBP and MAG was present in the vicinity of axons, although no myelin was visible in the conventional stains for myelin. In mld, GFA-staining astrocytes were present in normal numbers. MAG could be demonstrated in its normal localization along axons, but MBP was visible only in the comparatively old animal (85 days). Here, it was demonstrated in an abnormal site--the perikarya and the proximal parts of the processes of oligodendrocytes. Thin myelin sheaths present in this animal could not be stained for MBP.

Animals↗
PubMed1983

Pick's disease: an immunocytochemical study of neuronal changes. Monoclonal antibodies show that Pick bodies share antigenic determinants with neurofibrillary tangles and neurofilaments.

We used rabbit antisera to the 210,000; 155,000; and 70,000 mol. wt. neurofilament - polypeptides and monoclonal antibodies (BF 10; RT97) known to react with human neurofilaments in an immunohistochemical study of neuronal changes in Pick's disease and in senile dementia of the Alzheimer type. Pick bodies as well as neurofibrillary tangles and neurites showed strong reactivity with the monoclonal antibodies but remained unlabeled when treated with the rabbit polyclonal antisera. Our results indicate that the stained material in Pick bodies share antigenic determinants with neurofibrillary tangles and neurofilaments.

Aged↗
PubMed1983

Immunocytochemical investigations of some human leukodystrophies.

One case of each of the following human leukodystrophies was examined immunocytochemically with antisera against myelin basic protein (MBP), myelin associated glycoprotein (MAG) and gliofibrillary acidic protein (GFA): Metachromatic leukodystrophy (MLD), connatal adrenoleukodystrophy (ALD), sudanophilic leukodystrophy of the adult (SLD) and connatal Pelizaeus-Merzbacher disease (PMD). A case of canine globoid cell leukodystrophy (GLD) was also included under the assumption that this disease was the same in the dog as in man. It was shown that the storage process in MLD and GLD did not involve MBP or MAG and that the breakdown of myelin with the formation of fat granule cells containing droplets of neutral fat in ALD and SLS proceeds in a similar way as in experimental Wallerian degeneration. In PMD, MBP is present in the vicinity of axons not surrounded by a myelin sheath demonstrable with conventional means. The globoid cells of GLD could be demonstrated to be of non-astrocytic origin.

Adolescent↗
PubMed1983

Fracture and fracture separation of the proximal humerus in children: report of 136 cases.

The authors report 136 fractures involving the proximal humerus in children (two-thirds with fractures of the proximal metaphysis, one-third with fractures of the epiphyseal growth plate). Functional and anatomical results are studied in 52 patients with a mean follow-up of 5 years (range 1-18 years). The results are compared with reports in the literature. Usually, treatment should not be operative. Surgical approach in older children with irreducible lesions is discussed.

Adolescent↗
PubMed1983

[Pyogenic sacroiliac arthritis in children. Analysis and commentary apropos of 7 cases].

Seven cases of pyogenic arthritis of the sacro-iliac joint in children were observed between 1968 and 1981. Their analysis is discussed with the conclusions of the too much rare publications in the recent literature. Taking in to account the anatomic particularities the necessity of very precise roentgenographic technics in order to assess the sacro-iliac joint, especially in children because of incomplete ossification, the authors replace this pathology into the osteomyelitis in children. From the clinical aspects of the disease, with its typically localized pain, and its acute clinical and biological septicemic syndrome, the authors attempt to demonstrate that the classical idea of a delayed diagnosis, is not absolute. Even in this particular localization, an early "up to date" diagnosis is possible allowing rapid treatment with efficient antibiotherapy directed towards the responsible germ after their isolation (especially staphylococcus aureus). Indubitable progress has been gained with systematic radio-nuclide bone scanning in emergency.

Adolescent↗
PubMed1982

[Posttraumatic club-hand. Therapeutic problems (apropos of 5 cases)].

Progressive radial deviation after epiphyseal separation in children is a rare complication. It is related to an imbalance of residual growth potential of the lower ends of the radius and ulna together. The functional disability is generally moderate. 5 cases were observed by the authors, all of which were treated surgically. The mechanism of the deformity and the surgical techniques are described. These are lengthening of the radius, angulation osteotomy of the radius, shortening of the ulna or epiphysiodeses. In some cases, operative excision of a partial epiphyseal closure can be done provided that there has been a close follow up after the trauma and an early diagnosis.

Adolescent↗
PubMed1981

[Metaphyseal osteolysis. Unusual aspect of reflex algodystrophy in children (author's transl)].

Two children 7 and 14 years old respectively, presented with reflex neurovascular dystrophy. The salient feature of the disease consisted of osteolytic lesions of distal tibial and fibulal metaphyses. To out knowledge these were not previously reported. Complete recovery spontaneously occurred in a few weeks or months. Thus they do not require any treatment, which might be harmful.

Bone Resorption↗
PubMed1980

[Congenital pseudarthrosis of the clavicle. Apropos of 7 cases].

The authors report 7 new cases of congenital pseudarthrosis of the clavicule. Clinical features are bearing out classical data of litterature. The disease is discovered during first months by the way of a clavicular swelling which settles mostly on the right side. But according to the authors the surgical treatment ought to be systematically purposed, restoring a normal morphology and avoiding any functional trouble. It consists in resecting the pseudarthrosis and grafting. This act is easy if done early, the ideal age being around 3 or 5.

Adolescent↗
PubMed1979

[A new orthopedic apparatus for the treatment of congenital equinovarus clubfoot. The active-passive articulated splint. Critical study propos of 72 cases treated in our department].

The authors present a new equipment for orthopaedic assesment of club-foot. This "dynamic splint" aims to produce a self-correction of the deformity by the infant at time of each movement of his lower limb. With regard to 72 such deformities, concerning 47 children, they record the results after an average follow-up of more than 2 years. Criterions of results, clinical and chiefly radiological, more objectives, are classified into 3 parts : correction of varus of the foot, correction of adduction of the hind-foot, correction of equinus.

Child↗
PubMed1979

[Bone scanning in children: technic and indications. Apropos of 50 cases].

In children, radionuclide skeletal imaging with 99m Tc M. D.P. has been performed in 50 cases. Scans are done immediately, 20 minutes and three hours after injection. Osteomyelitis has been studied in 24 cases, Legg Perthes disease and benign bone tumors in the others. The scintigraphic patterns are very sensitive but not characteristic. However this exam seems to be essential in some orthopedic disorders of children.

Adolescent↗
PubMed1977

Detection of IgG antibodies to type-specific Pseudomonas aeruginosa lipopolysaccharides by solid-phase radioimmunoassay.

Previous studies have suggested that IgG serotype-specific antibodies are protective against infections with pseudomonas aeruginosa. In the present study, type-specific IgG antibodies to P. aeruginosa were detected by solid-phase radioimmunoassay in sera from 15 volunteers before and after vaccination with lipopolysaccharides from P. aeruginosa and from four patients with endocarditis due to P. aeruginosa. Significant type-specific increases in IgG antibody occurred after both vaccination and infection. The correlation coefficients comparing net counts per minute by solid-phase radioimmunoassay with hemagglutination titers in the 15 vaccinees were 0.940, 0.874, 0.792, 0.903, 0.882, 0.869, and 0.704 for serotypes 1--7, respectively.

Adult↗