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Biomedical subjects

R Klein

Publications and source records attributed to R Klein.

At least 757 records · Page 42Linked to original sources
PubMed1985

Characterization of human large granular lymphocyte subpopulations: comparison of the phenotype of NK cells and of interleukin 2-dependent progenitors of cytolytic effector cells.

Antigenically different subpopulations of human large granular lymphocytes (LGL) were identified according to their reactivity with monoclonal antibodies (MoAb). Antigen-positive and -negative subsets were isolated by immunoaffinity columns using a Sepharose 4B gel coupled with F(a')2 goat anti-mouse IgG or by flow cytometry cell sorting. The distinct LGL subsets were tested for natural killer (NK) activity against a panel of tumor targets: K562, Daudi, Alab; and for antibody-dependent cellular cytotoxicity (ADCC) against antibody-coated RL male 1 cells. LGL positively selected for any of the following phenotypic markers: B73.1+, OKM1+, OKT11+, and OKT10+ were highly cytotoxic, while B73.1- and OKM1- cells were completely devoid of NK activity. The OKT10- and OKT11- LGL subsets were occasionally cytotoxic, with low levels of reactivity. LGL subpopulations were also tested in a limiting dilution assay (LDA) for their capacity to proliferate in medium supplemented with interleukin 2 (IL-2) and to develop NK-like cytotoxic activity. The majority of proliferative progenitors have the following phenotype: OKT11+, OKM1-, B73.1-, and OKT10-, while the majority of progenitors for cytotoxic cells were OKT11+, OKM1+/-, OKT10+, and B73.1-. Results indicate that although B73.1+ cells can grow, the mature B73.1+ NK cells seem to be primarily derived in vitro from a small subset of less differentiated B73.1 pre-NK progenitors in the peripheral blood lymphocytes.

Antibodies, Monoclonal↗
PubMed1984

Women in the NHS.

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Administrative Personnel↗
PubMed1984

Ophthalmologic care for persons with diabetes.

The provision of ophthalmologic care to diabetic patients was examined in a large, geographically defined population-based study in southern Wisconsin. Of the total of 2,272 patients examined, 902 were taking insulin and were diagnosed before 30 years of age (younger onset) and 1,370 were diagnosed at or after 30 years of age (older onset). Approximately 26% of the younger-onset and 36% of the older-onset diabetic population had never had an ophthalmologic examination. Characteristics of the younger-onset and older-onset population associated with never having had an ophthalmologic examination included living in a nonmetropolitan county, being older at the time of diagnosis, having a shorter duration of diabetes, having fewer years of education, receiving their diabetes care from a family or general practitioner, and having better visual acuity. Eleven percent of younger- and 7% of older-onset persons with Diabetic Retinopathy Study high-risk characteristics for severe visual loss had never been seen or were seen more than two years before the time of the study. Because severe visual loss caused by proliferative diabetic retinopathy may be reduced by timely photocoagulation treatment, this study suggests that a large number of patients who would benefit from ophthalmologic care are currently not receiving it.

Adult↗
PubMed1984

On the spot advice.

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Hospitals, Psychiatric↗
PubMed1984

The Wisconsin epidemiologic study of diabetic retinopathy. II. Prevalence and risk of diabetic retinopathy when age at diagnosis is less than 30 years.

In a population-based study in southern Wisconsin, 996 insulin-taking, younger-onset diabetic persons were examined using standard protocols to determine the prevalence and severity of diabetic retinopathy and associated risk variables. The prevalence of diabetic retinopathy varied from 17% to 97.5% in persons with diabetes for less than five years and 15 or more years, respectively. Proliferative retinopathy varied from 1.2% to 67% in persons with diabetes for less than ten years and 35 or more years, respectively. For persons with diabetes of 10 years' duration or less, the Cox regression model relates the severity or retinopathy to longer duration, older age at examination, and higher levels of glycosylated hemoglobin. After ten years of diabetes, severity of retinopathy was related to longer duration, high levels of glycosylated hemoglobin, presence of proteinuria, higher diastolic BP, and male sex.

Adolescent↗
PubMed1984

The Wisconsin epidemiologic study of diabetic retinopathy. III. Prevalence and risk of diabetic retinopathy when age at diagnosis is 30 or more years.

In a population-based study in southern Wisconsin, 1,370 patients given diagnoses of diabetes at age 30 years or older were examined using standard protocols to determine the prevalence and severity of diabetic retinopathy and associated risk variables. The prevalence of diabetic retinopathy varied from 28.8% in persons who had diabetes for less than five years to 77.8% in persons who had diabetes for 15 or more years. The rate of proliferative diabetic retinopathy varied from 2.0% in persons who had diabetes for less than five years to 15.5% in persons who had diabetes for 15 or more years. By using the Cox regression model, the severity of retinopathy was found to be related to longer duration of diabetes, younger age at diagnosis, higher glycosylated hemoglobin levels, higher systolic BP, use of insulin, presence of proteinuria, and small body mass.

Aged↗
PubMed1984

Flow cytometry of postmortem human testicular tissue in cases of atherosclerosis.

From forty-seven autopsy cases of atherosclerosis flow cytometry (FCM) of DNA and histology of both testes are compared with the histological sections of their supplying vessels arteriae testiculares and arteriae ductus deferentis at different levels. By this method, changes of spermatogenesis are judged separately for each side and the results can be related to the local conditions of blood supply. Four young men, dead after traffic accidents, served as control. In the majority of cases, the computer-assisted evaluations of the meiotic DNA histograms show no differences between the right and left testis, even when differences of the arterial diameters are found by histology. On the other hand, cases with distinct differences in the histograms can show insignificant pathological alterations of the vessels. Though most excessive forms of macroscopic and microscopic atherosclerosis do not necessarily lead to a significant reduction in spermatogenesis, some cases with moderate forms show a strong reduction or even a total loss. This discrepancy can best be explained by superposition of other diseases.

Adult↗
PubMed1984

Ultrastructural changes in adrenaline- and SGC-cells after morphine coincide with alterations of adrenaline and dopamine levels.

The effects of morphine on chromaffin cell ultrastructure and catecholamine contents were studied using the adrenal glands from male mice (ICR strain). After 2 h adrenaline was increased 25% from 8.1 to 11.6 mumol/g tissue, followed by a 50% decrease to 5.2 mumol/g between 8-24 h and low values persisting at 72 h. Dopamine increased initially, reaching peak values of 0.5 mumol/g between 8-24 h, but had returned towards control values of 0.29 mumol/g by 72 h. Noradrenaline remained unchanged at 2.5 mumol/gram. Naloxone prevented alterations in adrenaline and dopamine levels. Ultrastructural examination revealed several types of catecholamine-storing cells. Of these the adrenaline and small-granule chromaffin (SGC) cells were more affected by morphine than noradrenaline cells. While the initial elevation of adrenaline 2 h after morphine was not accompanied by significant ultrastructural changes, the decrease after 8 and 24 h was paralleled by a significant (p less than 0.001) loss of adrenaline granules. Signs of active membrane turnover included an increase in the number of vacuoles, and the appearance of numerous coated omega profiles and coated (77.7 +/- 0.6 nm) vesicles. Clusters of synaptic-like vesicles (59.8 +/- 8.2 nm), slightly larger than neuronal vesicles (45.4 +/- 6.4), increased in the SGC-cells. After 72 h, the chromaffin granules in adrenaline cells remained low in numbers and were heterogeneous in electron density. Many synaptic-like vesicles were aligned along the SGC-cell membranes where only few chromaffin granules (109.3 +/- 20 nm) remained. Thus, continuous morphine exposure for 8-72 h increases the turnover of storage granules in adrenaline and SGC-cells with less effect on the noradrenaline cells which maintain catecholamine levels as indicated by biochemical analysis.

Adrenal Medulla↗
PubMed1984

Hypoglycemic therapy in patients diagnosed to have diabetes at 30 years of age or older.

Treatment patterns were investigated for a population of diabetic persons diagnosed at 30 years of age or older who received primary medical care in an eleven county area in southern Wisconsin. Of the 6074 patients who met the diagnostic criteria for diabetes, 51% received their primary care from internists, 35% from general practitioners, and 14% from family practitioners. Insulin usage was more frequent in patients with a longer duration of diabetes, younger age at diagnosis and poor recent glucose control. Prescription of oral medications increased with increasing current age and age at diagnosis. A greater proportion of the patients of internists than those of general practitioners were treated with insulin. Oral hypoglycemic agents were prescribed more frequently for patients of general practitioners than for patients of internists. These differences remained after controlling for duration, age at diagnosis, and status of recent glucose control. Therefore, practitioner type is associated with treatment prescribed for diabetic patients diagnosed after 30 years of age.

Administration, Oral↗