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Biomedical subjects

R King

Publications and source records attributed to R King.

At least 181 records · Page 10Linked to original sources
PubMed1988

Automatic microinjection system facilitates detection of growth inhibitory mRNA.

Naturally quiescent human lymphocytes, consisting predominantly of T cells, contain mRNA(s) that can inhibit DNA synthesis when injected into either human diploid fibroblasts (IMR-90) or transformed recipient cells (HeLa). By using an automated capillary microinjection system and a fluorescent coinjection marker (fluorescein isothiocyanate-dextran), individually injected cells can be retrieved and analyzed for DNA synthesis. mRNA isolated from resting T cells is able to block the cells from entering the S phase. The block is reversible and leads to a delay in DNA synthesis. The inhibitory effect is not observed if the injected mRNA is isolated from growth-activated T cells. The disappearance of the inhibition coincides with the approach of the G1/S boundary in both the donor T cells and the recipient human fibroblasts. The mRNA of resting T cells was size-fractionated and the peak inhibitory activity was recovered in a fraction approximately equal to 1.5 kilobases long.

Cell Division↗
PubMed1988

Genetic markers.

Explore the source record for details and available documents.

DNA Restriction Enzymes↗
PubMed1987

Treadmill exercise test and ambulatory measures in panic attacks.

Treadmill exercise test performance and ambulatory heart rate and activity patterns of 40 patients with panic attacks were compared with 20 age-matched controls (control group 1) and 20 nonexercising controls (control group 2). All patients underwent a symptom-limited exercise stress test. Panic attack patients and control group 1 wore an ambulatory heart rate/activity monitor for up to 3 days. Panic patients had a significantly higher heart rate at 4 and 6 METS than either control group. The max METS were 11.2 +/- 2.3, 13.5 +/- 2.3 and 11.2 +/- 1.8 for the panic attack patients and control groups 1 and 2, respectively. One panic patient had ischemia on the treadmill at 12 METS. Panic patients had a significantly higher standing heart rate than controls. Furthermore, 11 of 39 panic patients had tachycardia on standing compared with 3 of 40 controls. Panic attack patients had higher wake and sleep heart rates than control group 1, but the differences were not significant. These results are consistent with autonomic dysfunction in panic patients but may also be due to differences in physical conditioning. The treadmill can be useful for reassuring patients and for identifying the rare patient with ischemia on exercise.

Adult↗
PubMed1987

Serum phosphate and anxiety in major depression.

Serum inorganic phosphate was measured in 33 hospitalized patients with unipolar depression. As predicted, serum phosphate showed significant negative correlations with Minnesota Multiphasic Personality Inventory scales sensitive to anxiety (Psychasthenia: -0.33) and somatic concern (Hypochondriasis: -0.51). This is consistent with previous observations of relative hypophosphatemia in patients with panic disorder. Clinical consequences of relative hypophosphatemia and the possible roles of hyperventilation or elevated peripheral catecholamines as mediators of this effect are discussed.

Adult↗
PubMed1986

Bilateral internuclear ophthalmoplegia in systemic lupus erythematosus.

The case of a 25-year-old female with bilateral internuclear ophthalmoplegia as a presenting feature of systemic lupus erythematosus (SLE) is described. It is important to recognize that bilateral internuclear ophthalmoplegia may be an early manifestation of cerebral involvement in SLE in addition to its occurrence in multiple sclerosis.

Adult↗
PubMed1986

[Pathomorphology of leprosy].

Leprosy is a widely distributed infectious disease in countries with tropic or subtropic climate and the most frequent treatable polyneuropathy. Because the import into countries with temperature climate should be considered a review is given on different stages and forms of course. Special attention is paid to structural changes and to the significance of morphological studies in establishing the diagnosis.

Dapsone↗
PubMed1985

Serotonin and schizophrenia: correlations between serotonergic activity and schizophrenic motor behavior.

Increased serotonergic activity in animals has been associated with a variety of stereotyped motor behaviors. In addition, serotonin facilitates brainstem, reticular, and spinal motor neuronal activity implicated in the expression of these behaviors. This report presents positive correlations between both peripheral (platelet serotonin levels) and central (cerebrospinal concentrations of 5-hydroxy-indoleacetic acid) measures of serotonin metabolism and the symptom of peculiar or unusual mannerisms and posturing in schizophrenic patients. The findings are discussed in light of the animal behavioral correlates of increased serotonergic activity and the stereotyped affectomotor behavior seen in some schizophrenic patients.

Adult↗
PubMed1985

Effects of opiate antagonists on spatial memory in young and aged rats.

The effects of post-training opiate antagonist administration on spatial memory were assessed in young and aged male Long Evans rats. In Experiment I rats were trained to visit each arm of an eight-arm radial maze once in a session to obtain a food reward placed at the end of each arm. During training aged rats required significantly more trials to achieve criterion performance when compared to young mature rats. However, administration of the opiate antagonist naloxone (2.0 mg/kg) immediately after each training trial did not significantly alter the rate of achieving accurate performance in either age group. In Experiment II young and aged rats that were previously trained to a comparable criterion on the radial maze were tested on the same maze apparatus in novel spatial environments. When animals were exposed to novel spatial information, the effects of post-trial opiate antagonists were examined using a within-subjects counter-balanced design. In Experiment IIa naloxone (2 mg/kg) enhanced the performance of both young and aged rats. In Experiment IIB naltrexone (1.0 mg/kg) was found to have a comparable effect of enhancing the performance of both age groups. In addition, in Experiment IIb a significant age-related deficit was found in rats tested in novel spatial environments. These results indicate that opiate antagonists are capable of improving memory for new spatial information in both young and aged rats on a task that is sensitive to behavioral deficits during normal aging.

Age Factors↗
PubMed1985

Obtaining parental consent--opting in or opting out?

In a population based project aimed at identifying children with specified disabilities within a health region, there was, rightly, concern about the transfer of data on named children across health district boundaries. Two methods of obtaining parental consent for this process were tested. High recruitment rates were achieved using an 'opting out' approach.

Confidentiality↗
PubMed1985

[Return migration and the development of a rural commune in Basilicata].

"This study, based essentially on a survey of 80 returnees, examines the extent to which return migration has contributed to processes of economic change in an agro-town in southern Italy, Bernalda (Potenza)." The authors conclude that return migration has had only a limited impact on the economy because of the low demand for skilled labor in the area of origin. (summary in ENG, FRE)

Demography↗
PubMed1984

Gonadal tumors in patients with gonadal dysgenesis and sex chromosomal rings and fragments.

Patients with female phenotypes and dysgenetic gonads harboring testicular tissue have a markedly increased risk of developing gonadal tumors. Cytogenetic demonstration of Y chromatin is the currently accepted criterion for performing prophylactic gonadectomies in these women. We studied four patients with dysgenetic gonads containing either testicular tissue or germ cell tumors. All had small sex chromosomal fragments which could not be characterized by conventional cytogenetic studies. Clinical features, DNA replication studies, and immunologic assays of Xga and H-Y antigens failed to correlate consistently with the gonadal histology. We recommend prophylactic gonadectomies and subsequent hormone replacement in all patients with female phenotypes, gonadal dysgenesis, and cytogenetically indeterminate sex chromosomal fragments.

Adolescent↗
PubMed1984

Changes in the conformation of the interferon beta gene during differentiation and induction.

DNase I sensitivity was used to investigate the chromatin conformation of the interferon beta gene during differentiation of the mouse teratocarcinoma cell line PC13 . These cells do not produce interferon upon viral induction in their undifferentiated state, but do so on differentiation from stem cells to endoderm. Only in induced differentiated cells were the interferon beta genes digested by DNase I. A similar effect was seen in a line of human cells ( MG63 ) upon induction. We conclude that it is induction of interferon production that brings about the change in the DNase I sensitivity of these genes, rather than differentiation.

Animals↗
PubMed1984

Chaotic behavior in dopamine neurodynamics.

We report the results of the dynamics of a model of the central dopaminergic neuronal system. In particular, for certain values of a parameter k, which monitors the efficacy of dopamine at the postsynaptic receptor, chaotic solutions of the dynamical equations appear--a prediction that correlates with the observed increased variability in behavior among schizophrenics, the rapid fluctuations in motor activity among Parkinsonian patients treated chronically with L-dopa, and the lability of mood in some patients with an affective disorder. Moreover our hypothesis offers specific results concerning the appearance or disappearance of erratic solutions as a function of k and the external input to the dopamine neuronal system.

Brain↗