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Biomedical subjects

R Hicks

Publications and source records attributed to R Hicks.

At least 127 records · Page 7Linked to original sources
PubMed1971

Relationship between the anti-inflammatory and irritant properties of inflammatory exudate.

1. Using the rat paw oedema assay procedure a comparison was made between the anti-inflammatory and irritant properties of the inflammatory exudate obtained from polyester sponges implanted subcutaneously in adrenalectomized rats. Where necessary, comparison was also made with a known counter-irritant, carrageenin.2. A significant correlation between the anti-inflammatory and irritant properties of sponge exudate was observed when each parameter was determined in relation to dose. A similar result was obtained with carrageenin.3. A comparison of the two activities of sponge exudate samples harvested at various times following sponge implantation did not give a significant correlation although in each case significant anti-inflammatory activity was accompanied by marked irritation.4. Time-effect curves of the two activities of sponge exudate showed a significant correlation over the time period examined. However, no such correlation was obtained using carrageenin.5. Both activities of sponge exudate were retained following dialysis.6. These findings are discussed in relation to a counter-irritant mode of action for sponge exudate.

Adrenalectomy↗
PubMed1970

One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods.

The indirect fluorescent-antibody technique was used to examine 422 food samples for the presence of salmonellae. A cultural phase involving a 16-hr preenrichment in buffered nutrient broth-milk medium followed by a 4- to 5-hr subculture into fresh medium of the same composition was evaluated. This procedure yielded a sufficient population of salmonellae so that no false-negative results were obtained. Of the 31 false-positives obtained, 12 samples yielded positive cultural results upon extensive subculture of the original enrichment broths. Yeast cells and both vegetative and spore forms of bacilli were observed to fluoresce when stained with anti-Salmonella serum. Efforts to ascertain the cause of these cross-reactions and several alternate explanations are discussed.

Animals↗
PubMed1969

Immunofluorescent staining of Salmonella species with flagellar sera.

Salmonellae stained with flagellar sera by either the direct or indirect fluorescent-antibody technique fluoresced at the cell surface. Investigations showed that fimbriae, flagellar material, and capsule antigens were not the participating antigens in this reaction. Cell surface staining was inhibited by mannose but was unaffected by glucose, galactose, lactose, maltose, and sucrose. Specific absorption of the flagellar sera by intact cells and purified somatic antigen showed that O antigens were the site of the surface staining. The advantages of using flagellar sera in fluorescent-antibody screening of materials for the presence of salmonellae are discussed.

Animal Feed↗