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Biomedical subjects

R Hampl

Publications and source records attributed to R Hampl.

At least 163 records · Page 9Linked to original sources
PubMed1980

Receptor binding of B-norsteroids.

The binding of B-nor-analogues of glucocorticoids, estrogens and of an androgen to specific cytosol receptors was compared with that of parent compounds of the normal series. In the study of binding of B-norcortisol and related compounds rat liver cytosol was used, while in a case of B-norestrogens rat uterus cytosol and for 17 alpha-methyl-B-nortestosterone rat prostate cytosol was used. The displacement of the appropriate radioligands by B-norsteroids was remarkably lower than that caused by correspondinga steroids with normal B-ring with the exception of 17 alpha-methyl-B-nortestosterone.

Animals↗
PubMed1979

Radioimmunoassay of nortestosterone and related steroids.

A radioimmunoassay of nortestosterone and related steroids, including its principal metabolities, is described and evaluated. Antisera against nortestosterone-17 beta-hemisuccinate- and nortestosterone-3-carboxymethyloxime-bovine serum albumin were raised in goats. By using a mixture of such antisera with different selectivity, the cross-reactions of several naturally occurring steroids can be reduced. The method can be applied for the detection of nortestosterone in both unprocessed or hydrolyzed urine extracts and also in plasma. It has been used as a screening test for anabolics in doping control.

Animals↗
PubMed1978

Radioimmunoassay of 17-alpha-alkylated anabolic steroids.

A method for the detection of anabolic 17-alpha-alkylated androstane derivatives in both plasma and urine is described and evaluated. The goat and rabbit antisera against 17-alpha-Methyltestosterone-3-carboxymethyloxim-Rinder-serum albumin were raised and compared using [3H]methandrostenolone as a tracer. 22 Steroids including 10 potent synthetic anabolics were tested for their cross-reaction with these antisera.

Androstanes↗
PubMed1977

Binding of synthetic anabolic steroids to testosterone-estradiol binding globulin and to rat prostate cytosol.

The binding of five synthetic anabolic steroids and two natural androgens to testosterone-estradiol binding globulin (TeBG) and to cytoplasmic fraction containing androgen receptors from prostates of castrated rats was compared. The binding affinity to TeBg decreased in sequence: dihydrotestosterone--testosterone--methyltestosterone-methandienone--nortestosterone--dimethylandrostanolone--nortestosterone--phenylpropionate (no binding); in the prostatic cytosol the following fecreasing sequence was found: dihydrostestosterone--methyltestosterone--methandienone--nortestosterone--nortestosterone phenylpropionate--testosterone--dimethylandrostanolone. The law binding to TeBG of 17alpha-methylated steroids possessing at the same time high affinity to cytoplasmic receptors may enhance their effect in target tissues.

Anabolic Agents↗
PubMed1977

Aldosterone binding in bovine ciliary body.

The presence of aldosterone binding which was not identical with dexamethasone binding was verified in the ciliary body of the bovine eye in vitro. The site of aldosterone binding was presumably located in the non-pigmented layer of ciliary epithelium. Specific aldosterone binding was found also in lens epithelium but not in corneal epithelium. The finding of receptor-like aldosterone binding confirms the concept of possible mineralocorticoid-dependent active sodium transport through the ciliary and lens epithelium in the eye.

Aldosterone↗
PubMed1976

Indentification and radioimmunologic estimation of sexual steroid hormones in aqueous humor and vitreous of rabbit eye.

The occurrence of testosterone, estrone, and estradiol-17 alpha in aqueous humor and vitreous body of the rabbit eye was demonstrated. The identity of the steroid hormones was confirmed by their chromatographic properties, microreactions, and radiochemically by crystallization to constant specific activity of their labeled acetates. The levels of testosterone and estrogens in rabbit male and female plasma, aqueous, and vitreous were determined by radioimmunologic assay. The concentrations of the steroid hormones in plasma are higher than in aqueous and vitreous. However, testosterone in aqueous circulates in free form and lacks the binding to sexual hormone-binding globulin as in plasma.

Animals↗