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Biomedical subjects

R H Tomar

Publications and source records attributed to R H Tomar.

At least 55 records · Page 3Linked to original sources
PubMed1980

Human T lymphcytes are enriched for transfer factor.

Using a canine local skin assay, we tested transfer factor prepared from three subpopulations of human peripheral blood mononuclear cells. T lymphocytes are enriched for transfer factor whereas adherent cells and nonadherent/nonrosetting cells are not. Thus, it appears as if transfer factor is a T cell lymphokine, the immunobiology of which is still unclear.

Animals↗
PubMed1979

Treatment of rheumatoid arthritis with levamisole: long-term results and immune changes.

We treated 29 rheumatoid arthritis patients with levamisole. on the basis of a 25% improvement in any 3 of 6 measurements 95% of the patients had a favourable response within 20 weeks. However, 64% of the patients discontinued levamisole by 40 to 60 weeks because of rash or secondary treatment failures. Delayed skin reactivity to streptokinase-streptodornase increased significantly in the entire treatment group, but there was in inverse correlation between skin test enhancement and clinical response. There was no overall change in lymphocytes response to phytohaemagglutinin (PHA) after 4 and 16 weeks of treatment, but seven patients with enhanced lymphocyte responsiveness to PHA experienced an earlier clinical response to levamisole. Treatment with levamisole frequently results in clinical improvement in rheumatoid arthritis, but this is not clearly related to a stimulatory effect on cell-mediated immunity. Its long-term usefulness may be limited by a high incidence of relapse and rash.

Arthritis, Rheumatoid↗
PubMed1979

Mononuclear cells contain human transfer factor as assayed locally on the skin of dogs.

We prepared transfer factor from mononuclear (M) cells and polymorphonuclear (PMN) cells by sieving cell lysates through a P-2 Biogel column. By assaying column fractions by their ability to induce a local delayed-type reaction to antigen in canine skin, we determined that mononuclear cells most consistently contained skin activity. The elution patterns from P-2 Biogel of PMN or M-enriched preparations were less complex and much different from that seen with whole leukocytes.

Absorption↗
PubMed1978

Chronic idiopathic thrombocytopenic purpura. A familial immunodeficiency syndrome?

We studied three children with chronic idiopathic thrombocytopenic purpura (ITP) and their immediate families. All three patients and 10/13 family members manifested at least one immunologic defect, eg, decreased numbers of T lymphocytes (1/3, 5/13), diminished in vitro response to phytohemagglutinin (2/3, 6/13), dysgammaglobulinemia (2/3, 4/13), altered autoantibodies (1/3, 5/13), and decreased serum properdin levels (3/3, 2/13). In addition, one parent and two asymptomatic siblings of two of the propositi had shortened platelet life spans with normal platelet counts. The HLA antigens A3 and B7 were identified in all three families. There also appeared to be an association between a familial haplotype and the immunologic defects. Chronic ITP appears to occur in families with underlying immunologic defects, which are genetically related.

Adolescent↗
PubMed1976

Transfer factor: hypoxanthine is a major component of a fraction with in vivo activity.

Transfer factor was prepared from the leukocyte lysates of four donors with known skin test reactivity. After ultrafiltration and double-gel filtration on polyacrylamide gels, fraction IV of the preparation was found to have biologic activity. This fraction contained one major and occasionally one minor ultraviolet-absorbing and zero to one ninhydrin-detectable spots on thin-layer chromatography. The major ultraviolet spot was identified as hypoxanthine. Hypoxanthine was demonstrated to be responsible for the high 260 nm/280 nm ratio of preparations with biologic activity in vivo. It was not determined if hypoxanthine is required for transfer factor activity. In addition, an orcinol-negative preparation also had biologic activity.

Chromatography, Thin Layer↗
PubMed1976

Delayed skin reactor from streptokinase-streptodornase: stability studies and amino acid analysis.

Delayed skin reactor (DSR), the material in streptokinase-streptodornase responsible for eliciting delayed hypersensitivity reactions in man, is stable as a lyophilized powder or when stored at -70 degrees C. Storage at a pH above or below 6.0 leads to a decrease in the ability of DSR to stimulate lymphocyte proliferation in vitro. Its molecular weight is 30,000 as estimated by sodium dodecyl sulfate gel electrophoresis and it is composed of approximately 260 amino acid residues without apparent sulfhydryl-bridged subunits. The extinction coefficient of DSR (A 1% 280 nm) is 2.6.

Amino Acids↗
PubMed1976

Cellular requirements for simulation by the purified streptococcal antigen DSR.

Delayed skin reactor (DSR) was prepared from streptokinase-streptodornase. Peripheral blood mononuclear cells, were collected from a Ficoll-Hypaque gradient and subsequently subdivided into T cell-, B cell-, or monocyte-depleted populations. Mononuclear cells with complement receptors (B cells) were removed by a nylon-wool column adsorption. Sheep erythrocyte rosette forming cells (T cells) were removed by centrifugation on a second gradient. Populations of cells which did not adhere to plastic and/or ingest iron filings were monocyte-depleted. While response to DSR as measured by incorporation of tritiated thymidine into DNA was not affected by removing B lymphocytes, it was significantly diminished by removing T lymphocytes. Monocyte-depleted cells incorporated less tritiated thymidine into DNA in both antigen stimulated and control cells, but the stimulation index was not significantly altered.

Antigens↗
PubMed1975

Lupus erythematosus profundus following thrombocytopenic purpura.

A patient with thrombocytopenic purpura subsequently exhibited skin lesions of lupus erythematosus profundus, serositis, and serum antinuclear antibodies. Immunoglobulins and complement were found in the dermoepidermal junction of active skin lesions. Also of note was the presence of possible systemic lupus erythematosus in his mother.

Adolescent↗