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R H Haug

Publications and source records attributed to R H Haug.

82 records · Page 5Linked to original sources

Hyaluronidase production, lactose and salicin fermentation and phage-typability in bovine group B Streptococci.

Hyaluronidase, also called the spreading factor, may be an important pathogenic factor for the streptococci. Production of hyaluronidase is found in 75% of human clinical isolates of group B streptococci, and we have in our investigation found the same frequency (74%) in 195 bovine isolates. Of the same 195 isolates 17% turned out to be lactose negative, a characteristic usually regarded as being typical of group B streptococci of human origin. These same strains were also mostly hyaluronidase positive. The parameters investigated: hyaluronidase production, lactose and salicin fermentation and phage-typability, can be useful in tracing the origin of the group B. However, bovine and human streptococcal populations do not seem to be completely distinguishable because overlapping exists between the characteristics.

Animals↗

A comparison of two different methods for the serum opacity reaction of Streptococci group A. Occurrence of antibodies against opacity factor in human sera.

For detection of the opacity factor (OF) in streptococcus group A, two different methods, the slide technique and the blood agar technique, have been compared. The results agree closely. The reaction of OF positive strains on the blood agar medium seems, however, to be more difficult to interpret than the clear-cut opalescence in the slide reaction. Antibody to serum opacity factor was demonstrated in human sera using the solid agar method. Such antibodies were demonstrated in 26 out of a total of 46 ASO positive sera examined.

Agar↗

Pigment production in group B streptococci.

Most haemolytic group B streptococci produced a yellow to orange pigment in Colombia agar and in some Todd Hewitt broths. The composition of the broth medium was important. Trypsin inhibited pigment production, but had no effect on pigment already produced. Optical density measurement revealed three main peaks at 420, 260 and 215 nm respectively with pigment producing strains. Nonpigmented strains gave only the two peaks at 260 and 215 nm.

Culture Media↗

Comparison of the agglutination and the co-agglutination techniques in T-tying of Streptococcus pyogenes.

A comparison of two different methods in T-typing 200 Norwegian group A strains is reported. The traditional agglutination technique was compared with the co-agglutination method of Christensen and co-workers. Fifty-five strains could not be typed by either method. The typing results for 128 of the strains were in agreement. The most common T types were the folloiwng: 1, 4, 8, 12, 13, 28, and strains belonging to the T pattern 5/11/12/27/44.

Agglutination Tests↗