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Biomedical subjects

R Gupta

Publications and source records attributed to R Gupta.

At least 667 records · Page 37Linked to original sources
PubMed1990

Intestinal absorption of macromolecules and epithelial cell surface glycosylation in suckling rats nursed on mothers fed low-protein diet-I.

The effect of feeding 8% protein (low-protein; LP) diet to lactating rats has been studied on the absorption of macromolecules and the glycosylation of enterocytes in suckling animals. The absorption of 125I-labelled bovine serum albumin, gamma-globulin and alpha-lactalbumin was 24-314% more in pups nursed on mothers fed LP diet compared to the pair-fed controls. The observed enhancement in protein absorption was associated with a significant increase in the binding of these proteins to microvillus membranes (MVM). The sialic acid content of brush borders was unaltered but the fucose level was augmented (p less than 0.01) in pups nursed on rats fed LP diet. The binding of 125I-labelled wheat germ agglutinin and Ulex europeus agglutinin I to MVM was in agreement to the data on sialic acid and fucose levels of the membranes. The binding of peanut agglutinin to MVM was 30% low in pups from LP-diet-fed animals. The incorporation of (14C) D-mannose into MVM was essentially unaffected but that of (14C) N-acetylglucosamine was reduced in pups reared on mothers given LP diet. These findings suggest that the quality of maternal nutrition affects the absorption of macromolecules and the glycosylation in developing rat intestine.

Animals↗
PubMed1990

Differential effects of IGF-I and insulin on glucoregulation and fat metabolism in depancreatized dogs.

The effects of equipotent glucose-lowering doses of insulinlike growth factor I (IGF-I) and insulin on tracer-determined glucose kinetics and several metabolites were compared in 14 experiments (7 in each group) in fasted, totally depancreatized dogs. This model prevented variations in insulin secretion induced by IGF-I and permitted evaluation of the effects of IGF-I on extrapancreatic glucagon. Steady-state moderate hyperglycemia (9.9 +/- 0.2 mM) was maintained by a subbasal intraportal infusion of insulin (1.29 +/- 0.17 pmol.kg-1.min-1). This was continued throughout the experiment, allowing evaluation of IGF-I effects on insulin clearance. Human recombinant IGF-I or insulin was given intravenously as a primed infusion for 90 min, followed by a 50-min recovery period. The dose of IGF-I was a 2.6-nmol/kg bolus plus 57.4 pmol.kg-1.min-1. The insulin dose required to induce the same plasma glucose decline as IGF-I (44 +/- 6 vs. 43 +/- 5%, NS) was 9-12 times lower (0.06-nmol/kg bolus + 6.4 +/- 0.6 pmol.kg-1.min-1). However, the mechanism of this decline differed with IGF-I and insulin; glucose production was much less suppressed (25 +/- 9 vs. 42 +/- 11%, P less than 0.001) and glucose utilization was more stimulated (68 +/- 18 vs. 38 +/- 19%, P less than 0.05) with IGF-I.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1990

Salmonella senftenberg outbreak in a neonatal unit.

Salmonella senftenberg was isolated from the stools of 35 newborns between June, 1987 and September, 1988. Twenty nine (82.8%) babies were preterms and twenty six (74%) babies were less than 2,000 grams in weight. All of them were symptomatic within 6 days of life, 74.3% (26) within 4 days. All of them had loose stools and weight loss. Other features were sclerema, jaundice and paralytic ileus. The organism was sensitive to nalidixic acid (100%), amikacin (94.6%), gentamicin (72.9%), cephaloridine (56.7%), and chloramphenicol (29.8%) and resistant to benzyl penicillin and ampicillin. Babies showed good response to a combination of cephaloridine and amikacin along with supportive care. Five babies died, 4 of septicemic shock and one of pulmonary hemorrhage.

Cross Infection↗