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R Grillot

Publications and source records attributed to R Grillot.

At least 55 records · Page 3Linked to original sources
PubMed1994

Western blot detection of IgG anti-Aspergillus fumigatus elastase in sera of patients with aspergillosis.

Anti-Aspergillus fumigatus alkaline protease IgG was investigated by Western blot. Specific IgG antibodies were detected in sera of two of nine patients with proven or highly probable invasive aspergillosis and in sera of one of eight patients with proven or highly probable allergic bronchopulmonary aspergillosis. No response was obtained in sera of 23 control patients. The specific but transient IgG response to this induced enzyme does not recommend its use as sole serodiagnostic aid.

Antibodies, Fungal↗
PubMed1994

Typing of Candida krusei clinical isolates by restriction endonuclease analysis and hybridization with CkF1,2 DNA probe.

The use of restriction endonuclease analysis and Southern hybridization with our new CkF1,2 DNA probe, cold labeled with peroxidase, for the typing of Candida krusei isolates has been investigated. Fifty-five clinical samples isolated from forty-five patients hospitalized in eight centers, one environmental strain, and two reference strains were evaluated. Patterns were analyzed by a computer-assisted method and compared by numerical analysis. Clearer and less ambiguous patterns were obtained by restriction with endonuclease HinfI. It generated 9 to 14 (average, 11) well-separated fragments in the range of 6.5 to 2.0 kb. Both their numbers and sizes varied greatly among the strains studied. The CkF1,2 probe hybridized with one to seven fragments of HinfI patterns. A total of 48 distinct types were distinguished among the 58 strains studied. HinfI and CkF1,2 patterns showed similarities of less than 83 and 75% for unrelated strains and more than 91 and 100% for related strains, respectively. The methods showed 100% typeability, 98% reproducibility, and a discriminatory power of 1. C. krusei isolates from each patient were distinct, whether from one hospital or from different hospitals. Multiple isolates from the same patient were identical, both over time and at different anatomic sites. An endogenous origin is suggested for the colonizing and infecting isolates among the 45 patients. The CkF1,2 probe enhanced discrimination of the strains and provided a definitive comparison for strain identity. Genetic linkages between isolates were assessed at the subspecies level, and 12 clusters were delineated. A typing scheme is proposed for epidemiological studies of C. krusei.

Blotting, Southern↗
PubMed1994

Diagnosis of human hydatidosis: comparison between imagery and six serologic techniques.

Echography and/or chest radiograph in association with six serologic (immunologic) methods were tested for their ability to diagnose human hydatidosis. The immunologic techniques used were latex agglutination, counter immunoelectrophoresis, enzyme-linked immunosorbent assay (ELISA) with whole hydatid fluid and with antigen 5, a thermobabile lipoprotein that elicits the arc 5 precipitin line in immunoelectrophoresis. In this report, the results of examinations of 273 patients are presented. Of these, 243 cases were surgically proven to have hydatidosis, whereas 30 of the 273 that were strongly suspected of having hydatid cysts by radiology were shown to have other pathologies. The ELISA was more sensitive than the other methods, with 204 of 243 sera shown to be positive. The remaining 39 sera that gave false-negative results were tested using two recently developed methods, enzyme-linked immunoelectrodiffusion assay (ELIEDA) and immunoblotting (electrophoretic migration of hydatid fluid antigens under denaturing but nonreducing conditions, blotting, and immunoenzymatic assay). Immunoblotting, using our experimental conditions, had a greater sensitivity than the ELIEDA with this technique. Four of 37 sera showed one to three bands of 65, 12, and 8 kD. The sensitivity of the ELISA did not increase with either the size or type of cyst. Comparison of radiology with serology confirmed a good correlation between the two methods. Nevertheless, serology is more specific but less sensitive than imagery.

Adolescent↗
PubMed1994

[Invasive aspergillosis: management of mycological diagnosis].

Establishing the diagnosis of invasive aspergillosis consists primarily in the detection of Aspergillus hyphae, the invading form of the fungus, mainly from respiratory tract specimens and by cultural isolation. In this overview of the topic, we emphasise the major obstacles to the successful diagnosis of this fungal infection. The authors describe then the general procedures for histopathological and mycological diagnosis (samples, choice of method and the interpretation of results). Finally, they summarise, from literature the success rate in detecting Aspergillus, depending on the potentially infected sites and the specimens examined (broncho-pulmonary or extra-pulmonary).

Aspergillosis↗
PubMed1993

[Food-related fungal infection risk in agranulocytosis. Mycological control of 273 food items offered to patients hospitalized in sterile units].

In sterile hospital units filamentous fungal infections are an unacceptable risk in patients with prolonged agranulocytosis. The occurrence of 3 successive cases in the Sterile unit of University Hospital of Grenoble prompted us to investigate in all elements of sterile unit, and notably food-processing. Two cooked rice dishes given to these patients were found to contain a substantial amount of Aspergillus fumigatus and were probably responsible for these infections. A more detailed study was carried out to evaluate the degree of food contamination by fungi in sterile unit. The study included 97 cooked dishes which had been sterilized in a 250 degrees oven for 40 minutes and 176 industrially packed foods delivered in single packs (e.g. milk and its derivatives, sweets and drinks). Each foodstuff was mycologically examined 3 to 5 times in different packs according to a procedure used in food analysis laboratories and raised to the standards of mycological examination. In this way, a 3-monthly food testing protocol in sterile unit could be set up. This protocol has a dual purpose: regular checking of the cooked dishes chain and maximum diversification of the dishes.

Agranulocytosis↗
PubMed1992

[Rapid identification of Candida albicans: evaluation of "Rapidec albicans". Study of 444 yeast strains].

Rapid identification of Candida albicans is of great importance as it is the most frequently isolated yeast pathogen. Rapidec albicans, a new 2-h micromethod, performs two fluorescent enzymatic activities: hexosaminidase and proline arylamidase. A total of 444 yeast strains (334 from type culture collections and 110 from recent clinical isolates) were tested. The sensitivity was 98.5% and the specificity 95.8%. When only considering the clinical strains, 47/47 Candida albicans were identified by Rapidec albicans (sensitivity 100%) but only 43/47 by the germ tube test (sensitivity 91.5%). The specificities of the two tests were respectively 98.2% and 100%. This new system is therefore very efficient for the routine diagnosis of Candida albicans in the clinical field. It is easier and quicker than the germ tube test.

Candida albicans↗
PubMed1989

[Antifungigram: interpretation and choice of a technic].

Identification and in vitro susceptibility testing of fungi is necessary mainly in immunocompromised patients. So, after a survey of the epidemiological results, the authors specified the indications of susceptibility tests; their advantages and difficulties and the lots of variations related to the fungus, drug, medium and technic. The authors compared the more recent methods from their experience: susceptibility to 6 antifungals (amphotericin B, flucytosine and 4 imidazoles) was estimated in 122 yeast strains isolated from deep specimens by 5 marketed technics. They got a good correlation between Mycototal and Antifongigramme Pasteur, but the two other technics (Mycodisk and Neosensitabs) frequently showed unreliable results.

Antifungal Agents↗
PubMed1988

[Pulmonary and cerebral aspergillosis in Burkitt's lymphoma].

Multiple intracerebral aspergillus abscesses in a 5 year old boy with a Burkitt's lymphoma are described. The disease was fatal despite antifungal treatment. The diagnostic and therapeutic problems, the risk factors and preventive care are discussed.

Antifungal Agents↗
PubMed1987

[Deep candidiasis in agranulocytic patients: mycological and serological surveillance in 91 febrile episodes and management of an antifungal and antibacterial protocol].

This study took place between February 1984 and February 1985 in 76 febrile neutropenic patients. We evaluated the whole mycological and serological results, in 91 febrile episodes. We separated two groups: group I (acute leukemia, chronic myeloïd leukemia, medullar aplasia, lymphomas) and group II (chronic lymphoïd leukemia, Hodgkin disease, myelomas, solid tumors). Patients were included in this study if they developed a temperature of 38.5 degrees C or greater and if they had less than five hundred neutrophils per mm3. They were treated with mono or bi-antibiotherapy. If the patient remained febrile more than 72 hours, an antifungal therapy was added (Amphotericin B IV). Through these weekly results, we conclude: the high frequency of digestive tract candidiasis in such patients; disseminated candidiasis occurs only in the first group patients with fungal digestive colonization; the overall response rate in this study was 77 per cent. This underlines the interest of empiric treatment with amphotericin B IV.

Agranulocytosis↗
PubMed1981

[Comparative study of the pathogenicity of free-living amoebae in mice and their cytopathogenic effect in vitro].

The study of the experimental pathogenic effect on the mouse of 47 strains of free-living amoebas (Vahlkampfia, Naegleria, Hartmannella and Acanthamoeba) showed that 5 strains belonging all to the species A. polyphaga could induce a fatal meningo-encephalitis in the mouse. After axenisation of 12 strains of Acanthamoeba, the study of the cytopathogenic effect in vitro showed that all the strains studied were able to induce, sooner or later, the destruction of the cellular layer. But, using the same inoculum, the strains pathogenic for the animal, seemed to have a quicker cytopathogenic effect in vitro.

Amoeba↗
PubMed1980

[Free-living amoebae in the Grenoble area swimming pool water. Influence of th "winter-summer" use and of the sterilising procedure (author's transl)].

An epidemiological study concerning the search for and identification of free-living amoebae has been carried out on swimming pool water in the Grenoble area. Eleven establishments were surveyed over a period of one year, by taking samples of water every month. The results obtained show that the positive nature of the samples and the nature of the isolated species are influenced by the two factors being studied: The rhythm of the "Summer-Winter" use of swimming pools and the nature of the sterilising procedure, chlorine, bromine and ozone. The current sterilising conditions of the swimming pool water are discussed.

Amoeba↗
PubMed1975

[Epidemic of autochthonous hepatic and intestinal amebiasis in a place near Grenoble].

We had the opportunity of studying an epidemic of autochthonous amoebiasis occurring in the autumn of 1974 in a small town of 4000 inhabitants 30 km from Grenoble. Attention was originally attracted by the occurrence in this town of two cases of hepatic amoebiasis and one of intestinal amoebiasis identified by rectoscopy. Systematic investigations (coproctic examinations and serological tests for amoebiasis by indirect antibody fluorescence) were then carried out on everyone in the locality with digestive disorders which were possibly referable to amoebiasis, and on the other members of their families. A total number of 148 coproctic examinations were made and in two cases revealed the presence of vegetative forms of Entamoeba histolytica. In both cases the infestation provoked few symptoms (asthenia, vague abdominal discomfort, intermittent and apparently banal diarrhoea). On the other hand 20 out of 94 serological tests revealed positive results, 14 of which were equal to or greater than a titre of 1/100, a level at which all risks of non-specificity are virtually ruled out under our experimental conditions. Material reasons made it impossible to subject these cases to repeated faecal checks, but in two of them at least the rectoscopic appearances were very suggestive of subacute intestinal amoebiasis. Moreover, amoebic disease appears to be well confirmed by the results obtained among the patients as a whole by treatment with Metroinidazole. A variety of hypotheses on the origin of this epidemic have been put forward and then abandoned (market garden produce, receipt by certain families of exotic frut from overseas). In actual fact water seems to be the point of departure, for, although specimens of water taken at 7 different levels in the water supply system failed to reveal the presence of a single amoeba, bacteriological analyses during autumn 1974 showed signs of faecal contamination. The locality, which is situated at the foot of the Chartreuse massif, receives its water solely from springs but there is a holiday camp for the staff of an international airline situated above the main water catchment.

Dysentery, Amebic↗