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Biomedical subjects

R Glaser

Publications and source records attributed to R Glaser.

At least 235 records · Page 13Linked to original sources
PubMed1976

Superinfection epithelial nasopharyngeal carcinoma cells with Epstein-Barr virus.

Attempts were made to superinfect epithelial explant cell cultures prepared from nasopharyngeal carcinomas with Epstein-Barr virus. Virus-specific markers were observed in such cultures 3 days after superinfection. In addition, expression of Epstein-Barr virus early antigens was observed in epithelial cell explant cultures treated with iododeoxyuridine. The results suggest that epithelial cells of at least certain nasopharyngeal carcinomas possess the receptor for the Epstein-Barr virus and that the latent virus genome can be induced from epithelial cells prepared from such tumors.

Antigens, Viral↗
PubMed1976

[The role of carriers of intracellular fixed charges in the regulation of the resting potential of cells with ion pumps].

The living cell is considered as a nonideal phase, surrounded by an anion-permeable membrane in a stationary state. Ionic pumps maintain a nonequilibrium state for Na+ and K+. When assuming the water inside and outside the cell to be in thermodynamic equilibrium and the cell without wall not to resist the hydrostatic pressure difference, it is possible to calculate a membrane potential which strongly depends on the concentration of charged nonpenetrating molecules inside the cell. Living cells with a high resting potential should therefore contain a high inner charge density (nerve cells) or must be resistant to an inner hydrostatic pressure (plant cells). Cells in isotonic solutions with low ionic strength are not able to attain a stationary state. The regulations derived here are applied to the concrete conditions of human red cells.

Calcium↗
PubMed1975

3-Hydroxymethyl-s-triazolo[3,4-a]phthalazine, a novel urinary hydralazine metabolite in man.

The elucidation of the structure of a new major metabolic product of hydralazine, 3-hydroxymethyl-s-triazolo[3,4-a]-phthalazine, is described. The structures of several other previously described metabolites of the drug, phthalazone, s-triazolo[3,4-a]phthalazine, and 3-methyl-s-triazolo[3,4-a]phthalazine, are confirmed. A metabolic pathway of hydralazine is also proposed.

Humans↗
PubMed1974

Host cell regulation of induction of Epstein-Barr virus.

When Epstein-Barr virus (EBV) negative cells (Raji) were treated with iododeoxyuridine, only the early antigen (EA) component was induced. There was no significant increase in EBV DNA and no virus particles were observed. Somatic-cell hybrids were prepared from the fusion of Raji and D98 cells (D98/Raji). When these cells were treated with iododeoxyuridine, early antigen EBV DNA, and virus particles were synthesized. These data suggest cellular control over the expression of the EBV genome.

Antigens, Viral↗
PubMed1973

Epstein-Barr virus: detection of genome in somatic cell hybrids of Burkitt lymphoblastoid cells.

Somatic cell hybrids of Burkitt lymphoblastoid cells, from which Epstein-Barr virus can be recovered, were examined for the presence of virus DNA by DNA-RNA hybridization. Four clones of hybrid cells, each negative for virus antigens by immunofluorescence, contained virus DNA in varying genomic equivalents. The number of virus genome equivalents increased in the hybrid cells after induction of virus with iododeoxyuridine.

Animals↗
PubMed1973

Effect of dibutyryl cyclic AMP on the induction of Epstein-Barr virus in hybrid cells.

Treatment of Epstein-Barr virus (EBV) negative somatic cell hybrids with 5'-iododeoxyuridine (IUdR) induced synthesis of EBV antigens and virus particles. When dibutyryl cAMP (Bt(2)-cAMP) was present in medium after exposure of cultures to IUdR, the incidence of cells synthesizing EBV early and virus capsid antigens was increased. The time necessary for appearance of EBV particles after induction by IUdR was significantly reduced in the presence of Bt(2)-cAMP. This enhancement was evident to a lesser degree with 3':5' cAMP than with Bt(2)-cAMP and did not occur with any other of the related compounds tested. The response observed was dose dependent. Untreated (no IUdR) EBV negative hybrid cells exposed to Bt(2)-cAMP also synthesized EBV antigens. The concentration of intracellular cAMP may act as one of the control mechanisms selecting for gene expression in this system.

Adenosine↗