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Biomedical subjects

R D Goldman

Publications and source records attributed to R D Goldman.

161 records · Page 9Linked to original sources
PubMed1973

Preservation of normal behavior by enucleated cells in culture.

BHK-21 fibroblasts and BSC-1 epithelial cells were enucleated with cytochalasin B. The enucleated cells were trypsinized and replated; their subsequent behavior was monitored by light optical techniques. The results demonstrate that the information necessary for attachment, spreading, shape formation, pinocytosis, contact inhibition, and cell locomotion is present in enucleated cytoplasm.

Animals↗
PubMed1972

The effects of cytochalasin B on the microfilaments of baby hamster kidney (BHK-21) cells.

Attempts were made to test the motile functions of bundles of microfilaments found in baby hamster kidney (BHK-21) cells, by using cytochalasin B (CB). It was found that individual cells respond differently to the drug. These differential effects are quite obvious in both light and electron microscope preparations. Some cells contain normal bundles of microfilaments even after 24 hr in CB, and other cells form muscle-like configurations which also contain arrays of microfilaments. These varied effects suggest the existence of several types of microfilaments in BHK-21 cells, and make the interpretation of the motile role of microfilaments difficult to evaluate at the present time.

Animals↗
PubMed1971

The role of three cytoplasmic fibers in BHK-21 cell motility. I. Microtubules and the effects of colchicine.

Microtubule breakdown in the presence of 5 or 40 microg/ml of colchicine is observed in BHK-21/C13 fibroblast-like cells. Several morphological and physiological effects are noted in the absence of microtubules: (a) the cells transform from fibroblast-like to epithelial-like cells; (b) the normal pattern of intracellular birefringence changes and a juxtanuclear cap of birefringent filaments is formed; (c) time-lapse cinematography demonstrates that cell locomotion is inhibited in colchicine-treated cells, even though membrane ruffling persists. The results are discussed in terms of the specific roles of microtubules in cultured cell motility and possible functional relationships of the three types of cytoplasmic fibers seen in BHK-21 cells.

Animals↗