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Biomedical subjects

R D Goldman

Publications and source records attributed to R D Goldman.

At least 145 records · Page 8Linked to original sources
PubMed1978

BHK21 myosin: isolation, biochemical characterization and intracellular localization.

Myosin has been isolated from baby hamster kidney cells (BHK21/C13) in high yield and characterized biochemically and immunologically. The subunit composition consists of 2 heavy chains, approximately 200,000 Daltons each, and 2 classes of light chains of approximately 16,000 and 20,000 Daltons. The myosin exhibits ATPase activity in the presence of K+-EDTA or Ca2+ but very little activity with Mg2+-ATP. The Mg2+-ATPase activity is stimulated only about 2-fold by skeletal actin, but a much larger activation is obtained in the presence of a protein kinase isolated from chicken gizzard. The increase in actin activation is accompained by the phosphorylation of the 20,000-Dalton light chain. BHK21 myosin is insoluble at low ionic strength and forms typical biopolar thick filaments. A specific antiserum generated against this protein forms a single precipitin line with the antigen but does not crossreact with either skeletal or smooth muscle myosin. The antiserum also specifically stains stress fibres in BHK21 cells as shown by indirect immunofluorescence.

Adenosine Triphosphatases↗
PubMed1977

Isolation and preliminary characterization of 10-nm filaments from baby hamster kidney (BHK-21) cells.

A procedure for isolating 10-nm filaments as juxtanuclear caps from normal and colchicine-treated BHK-21 cells is described. These aggregates of 10-nm filaments retain their birefringence and their structural integrity. The major proteins comprising the filament preparations can be resolved as two bands on sodium dodecyl sulfate gels, corresponding to approximate molecular weights of 54,000 and 55,000.

Cell Line↗
PubMed1977

Myosin subfragment binding for the localization of actin-like microfilaments in cultured cells. A light and electron microscope study.

Fluorescein-labeled heavy meromyosin subfragment-1 (F-S-1) has been purified by ion exchange chromatography and characterized in terms of its ability to bind specifically to actin. F-S-1 activates the Mg++-adenosine triphosphatase activity of rabbit skeletal muscle actin and decorates actin as shown by negative stains and thin sections of rabbit actin and rat embryo cell microfilament bundles, respectively. Binding of F-S-1 to cellular structures is prevented by pyrophosphate and by competition with excess unlabeled S-1. The F-S-1 is used in light microscope studies to determine the distribution of actin-containing structures in wnterphase and mitotic rat embryo and rat kangaroo cells. Interphase cells display the familiar pattern of fluorescent stress fibers. Chromosome-to-pole fibers are fluorescent in mitotic cells. The glycerol extraction procedures employed provide an opportunity to examine cells prepared in an identical manner by light and electron microscopy. The latter technique reveals that actin-like microfilaments are identifiable in spindles of glycerinated cells before and after addition of S-1 or HMM. In some cases, microfilaments appear to be closely associated with spindle microtubles. Comparison of the light and electron microscope results aids in the evaluation of the fluorescent myosin fragment technique and provides further evidence for possible structural and functional roles of actin in the mitotic apparatus.

Actins↗
PubMed1976

Localization and organization of microfilaments and related proteins in normal and virus-transformed cells.

The localization and organization of actin-like microfilaments in normal, SV-40 and adenovirus transformed cells are determined by the coordinated use of light optical, electron optical and biochemical techniques. In adenovirus-type 5 transformed hamster embryo cells, microfilament meshworks appear to be the predominant organizational form of cellular action, while in normal hamster cells, microfilament bundles are prevalent. Differences between 3T3 and SV-40 transformed 3T3 cells are less apparent and may be related to the packing and intracellular distribution of microfilament bundles. Attempts at relating these ultrastructural changes in transformed cells to the images obtained following reaction with fluorescein-labelled myosin fragments and indirect immunofluorescence with smooth muscle myosin antibody are discussed. In several instances the fluorescence microscope images to not correspond to the ultrastructural observations. The results are discussed in terms of the possible relationships between alterations in cytoplasmic contractile elements and the abnormal behavior of transformed cells.

Actins↗
PubMed1976

Left-handedness and cognitive deficit.

Intellectual and performance measures were taken on 7688 school children tested on three behavioral measures of handedness and one measure of eyedness. Test results were compared against all combinations of handedness and eyedness and against a measure of socio-economic level. No relationships of any kind were found. Comparisons of the present results are made against 33 studies concerned with possible deficits associated with left-handedness. The results of the present study combined with a review of the majority of studies on deficit and handedness strongly suggest that the hypothesis of no difference in intellectual and cognitive performance between right- and left-handed subjects can be accepted as true.

Attention↗
PubMed1976

The WISC may not be a valid predictor of school performance for primary-grade minority children.

In the present investigation we studied the validity of the Wechsler Intelligence Scale for Children (WISC) for predicting several criterial teachers' ratings for primary-grade black, Anglo, and Mexican-American children. The results were rather striking: although validities for the combined groups were good (rs near .6), the validities calculated within each group presented a different picture. Validities were good for the Anglo children but near zero for the black and Mexican-American children. These results suggest that the WISC may be of little value in the assessment of the educability of minority children. The implications of these results for educational placement and the heredity-environment controversy were discussed.

Achievement↗