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Biomedical subjects

R C Wright

Publications and source records attributed to R C Wright.

At least 37 records · Page 2Linked to original sources
PubMed1984

A new selective and differential agar medium for Escherichia coli and coliform organisms.

An enriched lauryl sulphate-aniline blue agar medium which is selective for Escherichia coli and coliform organisms is described. From faecal samples, the medium gave higher counts of colonies producing acid from lactose than media containing bile salts. From contaminated water and food samples, the medium gave comparable or higher counts of colonies identified as E. coli than standard media. Colonies of E. coli were more readily differentiated from those of other coliform organisms.

Agar↗
PubMed1983

The sterilizing capacity of propylene oxide and chlorhexidine diacetate solutions upon pre-injection swabs saturated with propan-2-ol.

The sporicidal activity of two solutions which have been used in the production of 'sterile' pre-injection swabs has been investigated. Propylene oxide (3.4% w/w) and chlorhexidine diacetate (0.6% w/w), both made up in approximately 65% w/w propan-2-ol, were artificially contaminated with spores of Bacillus subtilis var. niger and swabs were impregnated with these; surviving spores were enumerated at various temperatures and times of storage. Chlorhexidine diacetate had no sporicidal activity, whereas the activity of propylene oxide was dependent on temperature. The former should not be considered a sterilant and the latter can only be considered such if a controlled incubation period at an elevated temperature is employed.

1-Propanol↗
PubMed1982

A medium for the rapid enumeration of Escherichia coli in the presence of other faecal coliforms in tropical waters.

A selective membrane filtration medium is described for use in the rapid assessment of water quality in tropical countries where the incidence of faecal coliforms other than E. coli presents problems in the interpretation of results. The medium gives comparable results to MPN values obtained in the multiple tube dilution test using modified Gray's glutamate medium, and to membrane filtration counts obtained using M-FC broth and membrane-enriched Teepol broth, whilst differentiation of E. coli is enhanced.

Bacteriological Techniques↗
PubMed1982

A comparison of the levels of faecal indicator bacteria in water and human faeces in a rural area of a tropical developing country (Sierra Leone).

The levels of faecal coliforms (FC), indole-positive FC (presumptive Escherichia coli), faecal streptococci (FS), Streptococcus faecalis and Clostridium perfringens in the natural water sources used by 29 rural settlements in Sierra Leone were investigated. Levels of the same indicators in human faeces were also investigated. The incidence of Salmonella spp. in both habitats and the temperature, pH and conductivity of water sources were also recorded. All water sources were contaminated with the indicator bacteria, mean numbers of which occurred in the relationship FC greater than presumptive E. coli congruent to FS congruent to C. perfringens greater than S. faecalis. FC were also predominant in human faeces, the relationship of means being FC congruent to presumptive E. coli greater than FS greater S. faecalis greater than C. perfringens. The need for confirmation of FC counts obtained from water sources was indicated by the large number of positive tubes produced in the FC multiple-tube dilution test from some samples which could not be confirmed as presumptive E. coli. Salmonella spp. Were isolated from 13 water sources and 6% of faecal samples. Mean water temperature was high (26.2 degrees C), pH low (5.04) and conductivity low (34 microS cm-1). Presumptive E. coli was considered the most appropriate indicator of faecal pollution of the types of water investigated.

Bacteria↗
PubMed1980

Variability of glutathione S-transferase of human erythrocytes.

Glutathione transferase (GST) of human erythrocytes, while homogeneous upon electrophoresis, varied more than sixfold in amount in individuals. The levels of this enzyme were found to be inherited, but no practical way was devised to use this enzyme as a genetic marker.

Erythrocytes↗
PubMed1979

Kinetics and equilibria of pyrimidine nucleoside monophosphate kinase from human erythrocytes.

The common type of pyrimidine nucleoside monophosphate kinase (ATP:CMP phosphotransferase, EC 2.7.4.14), purified 50 000-fold from human erythrotes, reacted with a wide variety of nucleotides, but only ATP, dATP, UMP and CMP were good substrates. The optimum Mg2+ concentration, 2-3 mM, was generally independent of substrate concentration, of the nature of the substrate, and of the direction of the reaction. Kinetic studies indicated that a ternary complex was formed, that the substrates were bound at two unlike sites, and that the order of addition of substrates was random. Equilibrium constants were ATP + UMP 0.98, ATP + CMP 1.59, dATP + UMP 1.13, and ATP + AMP 1.20.

Adenosine Triphosphate↗
PubMed1979

Genital bacteriology: a comparative study of premenopausal women with postmenopausal women.

Premenopausal women are reportedly at a higher risk than postmenopausal women of postoperative infection following vaginal hysterectomy. A study was designed to determine whether a relationship exists between bacterial flora and patient age that may explain the difference in the risk of postoperative infection. No statistically significant difference was found in the number or type of bacterial species isolated from the cervix or vagina of premenopausal and postmenopausal women. Postmenopausal women on a regimen of conjugated estrogens had genital flora similar to that of the other women studied.

Adult↗
PubMed1979

Preoperative hot conization of the cervix: a possible method to reduce postoperative febrile morbidity following vaginal hysterectomy.

Laboratory results indicate that the endocervix may be a source of bacterial contamination when vaginal hysterectomy is performed. In a series of 160 consecutive vaginal hysterectomies in premenopausal women, hot conization of the cervix was performed prior to the scrub with an iodophore. No preoperative antibiotics were used in this series. The postoperative febrile morbidity rate was 4.3 per cent and the average stay was 4.5 days. These results are compared with those of three other groups: (1) patients who received a three-dose parenteral prophylactic antibiotic course with the first dose two hours prior to surgery had a febrile morbidity rate of 8.6 per cent. (2) In patients who had prophylactic antibiotics for five days with the first dose given intraoperatively, the febrile morbidity rate was 10.1 per cent. (3) The febrile morbidity rate in the group with no antibiotic prophylaxis or hot conization was 49.1 per cent. Laboratory and clinical data suggest that preoperative conization may be effective in the reduction of postoperative febrile morbidity.

Anti-Bacterial Agents↗
PubMed1979

Subcorneal pustular dermatosis and crippling arthritis.

A 25-year-old woman has suffered with subcorneal pustular dermatosis and a rheumatoid-like arthritis for the past 11 years. Both her skin lesions and arthralgia have abated with institution of dapsone administration and have worsened with interruption or reduction of the dose. The coexistence of arthritis and subcorneal pustular dermatosis and the response of both to therapy suggest a probable association.

Adult↗