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Biomedical subjects

R C Fowler

Publications and source records attributed to R C Fowler.

65 records · Page 4Linked to original sources
PubMed1963

IDENTIFICATION OF MYCOPLASMATACEAE BY THE FLUORESCENT ANTIBODY METHOD.

Clark, Harold W. (The George Washington University, Washington, D.C.), Jack S. Bailey, Richard C. Fowler, and Thomas McP. Brown. Identification of Mycoplasmataceae by the fluorescent antibody method. J. Bacteriol. 85:111-118. 1963.-The conditions of the fluorescent antibody reactions were studied in relation to their application to Mycoplasmataceae or pleuropneumonia-like organisms (PPLO). Mycoplasma hominis type 1 and 2 antigens and their homologous antisera were used to determine the activity and specificity of these and other strains. Fluorescein isothiocyanate conjugated antiserum globulin preparations were used in both the direct and indirect fluorescent antibody methods. A direct tube technique was used for the detection and measurement of growth in broth cultures by the addition of conjugated antiserum. The specific fluorescent staining and recognition of hot water fixed M. hominis colonies was presented as a suitable identification standard. The antigenic activity was found to remain in the insoluble residue after exposure of M. hominis strains to sonic vibration (9 kc) for 30 min and centrifugation. Brief 2-min exposures of tissue cells to vibration (9 kc) caused the disruption of tissues, with the release of viable and "bound" nonwashable strains that reacted specifically with fluorescent antibody. It is proposed to apply both the sonic vibration and the fluorescent antibody techniques for the identification of Mycoplasmataceae in human tissues.

Journal Article↗
PubMed1961

Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area.

Bailey, Jack S. (George Washington University, Washington, D. C.), Harold W. Clark, William R. Felts, Richard C. Fowler, and Thomas McP. Brown. Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J. Bacteriol. 82:542-547. 1961.-Antigens were prepared from several tissue culture and human genital strains of pleuropneumonia-like organisms (PPLO) by a method utilizing continuous agitation of the incubating cultures. Antisera were produced in rabbits by intravenous injection of suspensions of these organisms standardized turbidimetrically. The antigenic properties of the selected strains were compared by agglutination techniques supplemented by a test based upon the inhibition of growth of PPLO by specific antisera.The majority of tissue culture strains of PPLO studied, including contaminants from several HeLa cell lines, appeared to be antigenically similar to the human type 1 strains. However, one strain (Sp-1) from a HeLa cell line was found to be related to the human type 2 PPLO.

Cell Line↗
PubMedDate not supplied

Classification of alcoholism with reference to comorbidity.

Classification of individuals with alcoholism is currently limited. The purpose of this analysis was to determine whether antisocial personality disorder and other primary psychiatric syndromes identified subgroups of alcoholics with differing characteristics. Alcoholic probands (n = 224) with alcoholism were divided into those with primary alcoholism (n = 128), antisocial alcoholism (n = 50), and secondary alcoholism (n = 46). These groups were evaluated with regard to alcohol-related symptoms and upon a variety of psychiatric signs and symptoms. The secondary alcoholism. The antisocial alcoholic and primary alcoholic groups demonstrated many similarities, but overall, the antisocial alcoholic group appeared most severe. The antisocial alcoholic group additionally exhibited a dissociation between the subjectively reported and the observed behavior. These findings support the concept of heterogeneity within the alcoholism spectrum. Longitudinal data are needed to determine whether the observed cross-sectional differences predict outcome differences.

Adult↗
PubMedDate not supplied

Pineal gland calcification and defective sense of direction.

Calcification of the pineal gland is shown to be closely related to defective sense of direction. In a tricentre prospective study of 750 patients lateral skull radiographs showed that 394 had calcified pineal glands. Sense of direction was assessed by subjective questioning and objective testing and the results noted on a scale of 0-10 (where 10 equals perfect sense of direction). The average score for the 394 patients with pineal gland calcification was 3.7 (range 0-8), whereas the 356 patients without pineal gland calcification had an average score of 7.6 (range 2-10). This difference was highly significant (p less than 0.01). A smaller parallel study in pigeons showed that pineal calcification also leads to a reduction in homing abilities. The findings suggested that the pineal gland plays an important part in directional sense and that damage to the gland, as indicated by calcification, causes defective sense of direction - perhaps by altering the intrinsic intracranial electromagnetic environment and thus affecting the magnetite response mechanism.

Age Factors↗
PubMedDate not supplied

Possible aluminum content of canned drinks.

Preliminary analyses by a commercial laboratory demonstrated varying amounts of aluminium in a small sampling of canned drinks, which had been stored at 15-20 degrees C., ranging from < 0.1 to 74 ppm depending on the product and storage time. Aluminum foil wrap immersed in several drinks demonstrated varying raters of dissolution from nearly zero to 100% over a 7 months periods when stored at an incubator temperature of 32-34 degrees C. Should aluminum prove to be of significant pathophysiologic consequence, some consideration should be given to all possible sources, including canned drinks which are consumed in copious quantities. When internal protective coatings are complete and remain intact, the cans stored properly and the contents consumed within a reasonable period of time, there appears to be little basis for concern about the ingestion of aluminum from the consumption of the canned drinks tested, as things now stand.

Aluminum↗