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Biomedical subjects

R Adler

Publications and source records attributed to R Adler.

At least 217 records · Page 12Linked to original sources
PubMed1979

Development, reactivity and GFA immunofluorescence of astroglia-containing monolayer cultures from rat cerebrum.

This report describes detailed protocols for the dissociation, seeding and growth in vitro of monolayer cultures derived from neonatal rat cerebrum. Primary cultures derived by using different seeding densities and in vitro ages were examined qualitatively and quantitatively for morphological composition in terms of two major cell classes (flat cells and process-bearing cells) and for the presence within these classes of glial fibrillary acidic protein (GFA) as detected by immunofluorescence histochemistry. Also examined was the reaction of the cells to serum withdrawal plus the administration of dibutyryl cyclic AMP in terms of the conversion of flat cells into process-bearing cells. Conditions are defined for the generation of in vitro cell populations, more than 90% of which are GFA-containing flat cells which can all be experimentally converted into cells with processes. These well-defined culture preparations will serve as useful models for future studies of astroglial behaviour.

Animals↗
PubMed1979

A procedure for purifying neuron-like cells in cultures from central nervous tissue with a defined medium.

A serum-free medium (N1) containing the supplements insulin, transferrin, progesterone, putrescine and selenium was used to culture cells from a variety of embryonic chick central nervous system tissues, namely, optic lobe, neural retina, spinal cord and telencephalon. The N1 medium supported the survival of fiber-bearing cells (features typical of cultured neurons) as well as or better than horse serum, while permitting no, or almost no flat cells. Survival and growth of chick flat cells required fetal calf, but not horse serum. Cultivation of newborn mouse telencephalon under these conditions yielded similar results, except that either fetal calf or horse serum supported flat cells.

Animals↗
PubMed1979

Pain in peripheral arteriography-a comparison of a low osmolality contrast medium with a conventional compound.

The pain reaction to a new low osmolality contrast agent, sodium-methyl-glucamine-ioxaglate, in comparison to the conventional compound methylglucamine-ioxitalamate was tested and statistically evaluated in 12 patients using the visual analogue scale method. The intensity of pain experienced was significantly smaller following intra-arterial injection of the compound of low osmolality as compared to the conventional contrast agent.

Adult↗
PubMed1979

Attitudes and interpersonal skills during pediatric internship.

The initial phases of a program for analyzing interpersonal skills and attitudes and personal adaptation of pediatric interns at a high-pressure tertiary care hospital are described. Interns found internship stressful and less satisfying in many regards than they expected. They were often unable to cope adaptively with the stresses encountered. There were indications of worsening attitudes toward aspects of patient care. However, interns' confidence increased during the year. A videotape vignette test of interpersonal sensitivity showed no change in sensitivity from beginning to end of the year. Videotapes of interns' visits with clinic outpatients made at three points during the year also showed no improvement in interpersonal or communication skills or attention to psychosocial issues. While parents were generally satisfied with the visit, interns lacked skills in some of these areas. None of the data collected, including sex, marital status, and pre-admission rank by the selection committee, predicted end-of-year performance rating by the chief residents. Sex and marital status were also uncorrelated with measured skills and attitudes.

Adaptation, Psychological↗
PubMed1978

[Therapy resisting pains. Drug therapy of cancer pain].

A pain may seem to resist all kinds of therapy without necessarily being absolutely refractory to treatment. The factors are discussed which may contribute to the "refractoriness" of a pain to therapy in the area of pain due to carcinoma, i.e. lack of understanding of modern pain concepts, inability to diagnose pain as due to conversion, failure to recognize the influence of the affects anxiety, hopelessness etc. on pain intensity, administration of analgesics in situations where another form of therapy would be indicated, e.g. plexus blockade, and insufficient knowledge of the effects, side effects, dosage and timing of the administration of mild analgesics, neuroleptics, antidepressives and narcotics.

Analgesics↗
PubMed1978

Complement receptors: specific detection by molecular complexes.

Immune complexes of BSA-anti BSA (IgG F(ab')2) will activate human complement by the alternate pathway. The C3 bound to these complexes binds to human peripheral blood mononuclear cells which bear complement receptors. This interaction is visualized by fluoresceinated antigen (BSA-FITC) or fluorescein conjugated antisera directed at the C3 component of complement. The assay appears to be more sensitive than the usual IgMEAC rosette technique and correlates well with the rosette method.

Animals↗
PubMed1978

Increased production of triglyceride-rich lipoproteins after partial biliary diversion in the rhesus monkey.

An interrelationship between bile acid and triglyceride metabolism is suggested by observations in patients with endogenous hypertriglyceridemia and in subjects treated with bile acid-binding resins or chenodeoxycholic acid. We investigated this possible interrelationship in the rhesus monkey. The effect of 14 days of partial bile diversion on plasma concentration and secretion rates of triglyceride-rich lipoproteins was studied by means of a stream-splitting device that permitted controlled interruption of the enterohepatic circulation. Diversion of 25% of the enterohepatic circulation resulted in increased bile acid synthesis and decreased bile acid secretion and pool size, and was associated with a 75% increase in fasting serum triglyceride concentration and a twofold increase in the rate of secretion of triglyceride-rich lipoproteins into plasma. Intraduodenal replacemtn of cholic acid during the period of bile diversion completely prevented the increase in lipoprotein secretion and partially prevented the increase in fasting plasma triglycerides, suggesting that these changes resulted from loss of bile acid rather than other components of bile. These studies show that partial removal of bile acids from the enterohepatic circulation augments the secretion of triglyceride-rich lipoprotein into plasma in the rhesus monkey. Elucidation of the mechanism of this effect may provide important new information concerning the control of hepatic lipoprotein secretion.

Animals↗
PubMed1978

Infection by herpes simplex virus and cells of nervous system origin: characterization of a non-permissive interaction.

Replication of herpes simplex virus type I (HSV-I) was studied in various cell lines of rat nervous system origin. Infection of neonatal rat glial primary cells with HSV-I, strain KOS, produced normal yields of progeny virus. Glioma lines B9 and B15 were permissive, the neuronal line B50 was partially restricted (10 to 100-fold reduction) and the neuronal line B103 was non-permissive (greater than 1000-fold reduction) for HSV-I (KOS) replication. Synthesis of virus DNA in infected B103 cells was not detected. However, at least some virus macromolecular synthesis was induced, including production of thymidine kinase, DNA polymerase and virus structural proteins.

Adsorption↗
PubMed1978

Possible role of Fc receptors on cells infected and transformed by herpesvirus: escape from immune cytolysis.

Receptors for the Fc portion of nonimmune immunoglobulin G were demonstrated on B103 rat brain neuroma cells infected with herpes simplex virus type 1 (HSV-1) KOS by a radioimmunoassay using 125I-labeled heat-aggregated Fc fragments. Immune F(ab')2 fragments specific for HSV antigens competed efficiently for Fc binding sites, suggesting that the binding of Fc fragments to infected cells is specific for viral cell-surface antigens. It has been suggested that the binding of immune complexes to Fc receptors on the surfaces of tumor cells in vivo plays a role in protecting these cells from immune destruction. In vitro evidence is presented for the ability of aggregated immunoglobulin G molecules bound to cell-surface Fc receptors to protect both HSV-infected and HSV-transformed cells against complement-dependent and cell-mediated immune lysis.

Animals↗
PubMed1978

Defense mechanisms and coping behavior in terminal illness. An overview.

Psychosomatic medicine is increasingly concerned with illness behavior, a concept which includes defense mechanisms and coping styles. An attempt was made to compare defensive processes and social adaptation in a group of 31 patients suffering from a probable terminal illness of iatrogenic origin. The surprising degree of conformity between the defense profiles towards the illness and those towards general life stress was found. Reaction-formation and stoicism were the defense mechanisms most often associated with successful defense. Interdependence of several ego measures such as defense against general life stress, defense against illness and social adaptation could be shown in various evaluations. Surprisingly, however, aggravators of illness turned out to be psychologically more stable than minimizers, a result which is in contrast to behavior in nonterminal illness. Theoretical implications of the results are discussed.

Adaptation, Psychological↗
PubMed1978

Thyrotoxicosis: comparison of effects of thyroid ablation and beta-adrenergic blockade on metabolic rate and ventilatory control.

To elucidate the role of the beta-sympathetic system in thyrotoxicosis (THY), we examined cardiac sensitivity to infused beta-agonist and compared the effect of beta-blockade with that of resolution of the hyperthyroid state. Beta-sympathetic (beta-SYM) sensitivity was measured as the heart rate response to isoproterenol in THY patients and in normal subjects. The patients with THY showed both lower threshold (P less than 0.05) and increased slope (P less than 0.05) of the heart rate-isoproterenol response, suggesting beta-SYM hypersensitivity. The beta-SYM like features of THY were measured in 7 patients before and 7 days after beta-SYM blockade with propranolol (mean dose 411 +/- 32 mg/day [SEM]) which was shown to block the heart rate response to isoproterenol. These results were compared with those in a similar group of thyrotoxic patients rendered euthyroid with 131I. During beta-SYM blockade, heart rate decreased from 101 +/- 6.3 to 78 +/- 4.6 (P less than 0.01), but the elevated metabolic rate (V02), resting ventilation (VE), and increased hypoxic and hypercapnic ventilatory responses were not significantly affected. In the group rendered euthyroid with 131I, heart rate decreased from 110 +/- 3.5 to 76 +/- 7.8 (P less than 0.02), but in contrast to the result of beta-SYM blockade, a 28% decrease in VO2 (P less than 0.01), 41% decrease in VE (P less than 0.05), a 38% decrease in hypercapnic ventilatory response (P less than 0.05), and a 66% decrease in hypoxic response (P less than 0.03) occurred. During THY, beta-SYM mechanisms are responsible for part of the tachycardia, but the metabolic and ventilatory abnormalities are not beta-SYM mediated.

Adult↗
PubMed1978

Acute pericarditis associated with hepatitis B infection.

Patients infected with hepatitis B have demonstrated a wide spectrum of clinical manifestations other than hepatitis. Immune complex formation has been proposed as a possible mechanism for such varied disease presentations. The present report describes a case in which acute pericarditis is associated with hepatitis B surface antigen-positive disease. Speculations are made relating the pericardial changes to the formation of immune complexes following hepatitis B virus infection.

Acute Disease↗