Biomedical subjects

Quan Zhang

Publications and source records attributed to Quan Zhang.

At least 55 records · Page 3Linked to original sources

PubMed2005

[Clinical analysis of sixteen cases of lymphoepithelial carcinoma of salivary gland].

BACKGROUND & OBJECTIVE: Lymphoepithelial carcinoma of salivary gland is a rare and special kind of malignant tumor, and has seldom been reported. This study was to summarize the clinical features, treatment and curative effect of this disease according to our experiences. METHODS: Clinical data of 16 patients with pathologically confirmed primary lymphoepithelial carcinoma of salivary gland, treated in Cancer Center of Sun Yat-sen University from Jan. 1990 to Sep. 2004, were retrospectively analyzed, and survival analysis was performed with Kaplan-Meier method. RESULTS: The 16 patients with lymphoepithelial carcinoma of salivary gland accounted for 3.6% of all the patients diagnosed as malignant tumors of salivary gland simultaneously in our center. All patients had tumors occurred in unilateral gland, aged 15-57 years, with the female to male ratio of 1:1. All patients were from southern China. Except for 1 patient, the rest 15 had Epstein-Barr virus (EBV) serological tests. The positive rates of EBV-VCA-IgA, EBV-EA-IgA, and EBV-DNA enzyme were 93.3%, 66.7%, and 86.7%, respectively. All patients were treated with surgery, of which 9 received surgery only, 5 received surgery plus postoperative radiotherapy, 1 received surgery plus postoperative chemoradiotherapy, 1 received surgery plus chemotherapy. Four (25.0%) patients had postoperative pathology-proved ipsilateral cervical lymph node metastasis. The 1-, 3-, and 5-year survival rates of the 16 patients were 86.15%, 48.46%, and 24.23%. CONCLUSIONS: Although lymphoepithelial carcinoma of salivary gland is poorly differentiated, the prognosis of this disease is good. Complete resection plus postoperative radiotherapy is possibly accommodating.

Adolescent↗

PubMed2004

Divergent potentials for cytoplasmic inheritance within the genus Syringa. A new trait associated with speciogenesis.

Epifluorescence microscopic detection of organelle DNA in the mature generative cell is a rapid method for determining the potential for the mode of cytoplasmic inheritance. We used this method to examine 19 of the known 22 to 27 species in the genus Syringa. Organelle DNA was undetectable in seven species, all in the subgenus Syringa, but was detected in the 12 species examined of the subgenera Syringa and Ligustrina. Therefore, species within the genus Syringa display differences in the potential cytoplasmic inheritance. Closer examination revealed that the mature generative cells of the species in which organelle DNA was detected contained both mitochondria and plastids, but cells of the species lacking detectable organelle DNA contained only mitochondria, and the epifluorescent organelle DNA signals from the mature generative cells corresponded to plastid DNA. In addition, semiquantitative analysis was used to demonstrate that, during pollen development, the amount of mitochondrial DNA decreased greatly in the generative cells of the species examined, but the amount of plastid DNA increased remarkably in the species containing plastids in the generative cell. The results suggest that all Syringa species exhibit potential maternal mitochondrial inheritance, and a number of the species exhibit potential biparental plastid inheritance. The difference between the modes of potential plastid inheritance among the species suggests different phylogenies for the species; it also supports recent conclusions of molecular, systematic studies of the Syringa. In addition, the results provide new evidence for the mechanisms of maternal mitochondrial inheritance in angiosperms.

Cytoplasm↗

PubMed2004

Salt cress. A halophyte and cryophyte Arabidopsis relative model system and its applicability to molecular genetic analyses of growth and development of extremophiles.

Salt cress (Thellungiella halophila) is a small winter annual crucifer with a short life cycle. It has a small genome (about 2 x Arabidopsis) with high sequence identity (average 92%) with Arabidopsis, and can be genetically transformed by the simple floral dip procedure. It is capable of copious seed production. Salt cress is an extremophile native to harsh environments and can reproduce after exposure to extreme salinity (500 mm NaCl) or cold to -15 degrees C. It is a typical halophyte that accumulates NaCl at controlled rates and also dramatic levels of Pro (>150 mm) during exposure to high salinity. Stomata of salt cress are distributed on the leaf surface at higher density, but are less open than the stomata of Arabidopsis and respond to salt stress by closing more tightly. Leaves of salt cress are more succulent-like, have a second layer of palisade mesophyll cells, and are frequently shed during extreme salt stress. Roots of salt cress develop both an extra endodermis and cortex cell layer compared to Arabidopsis. Salt cress, although salt and cold tolerant, is not exceptionally tolerant of soil desiccation. We have isolated several ethyl methanesulfonate mutants of salt cress that have reduced salinity tolerance, which provide evidence that salt tolerance in this halophyte can be significantly affected by individual genetic loci. Analysis of salt cress expressed sequence tags provides evidence for the presence of paralogs, missing in the Arabidopsis genome, and for genes with abiotic stress-relevant functions. Hybridizations of salt cress RNA targets to an Arabidopsis whole-genome oligonucleotide array indicate that commonly stress-associated transcripts are expressed at a noticeably higher level in unstressed salt cress plants and are induced rapidly under stress. Efficient transformation of salt cress allows for simple gene exchange between Arabidopsis and salt cress. In addition, the generation of T-DNA-tagged mutant collections of salt cress, already in progress, will open the door to a new era of forward and reverse genetic studies of extremophile plant biology.

Abscisic Acid↗

PubMed2004

Heterogeneous pollen in Chlorophytum comosum, a species with a unique mode of plastid inheritance intermediate between the maternal and biparental modes.

The majority of angiosperms display maternal plastid inheritance. The cytological mechanisms of this mode of inheritance have been well studied, but little is known about its genetic relationship to biparental inheritance. The angiosperm Chlorophytum comosum is unusual in that different pollen grains show traits of different modes of plastid inheritance. About 50% of these pollen grains exhibit the potential for biparental plastid inheritance, whereas the rest exhibit maternal plastid inheritance. There is no morphological difference between these two types of pollen. Pollen grains from different individuals of C. comosum all exhibited this variability. Closer examination revealed that plastid polarization occurs, with plastids being excluded from the generative cell during the first pollen mitosis. However, the exclusion is incomplete in 50% of the pollen grains, and the few plastids distributed to the generative cells divide actively after mitosis. Immunoelectron microscopy using an anti-DNA antibody demonstrated that the plastids contain a large amount of DNA. As there is a considerable discrepancy between the exclusion and duplication of plastids, resulting in plastids with opposite fates occurring simultaneously in C. comosum, we propose that the species is a transitional type with a mode of plastid inheritance that is genetically intermediate between the maternal and biparental modes.

Asparagaceae↗

PubMed2004

Capacitance measurements of exocytosis in mouse pancreatic alpha-, beta- and delta-cells within intact islets of Langerhans.

Capacitance measurements of exocytosis were applied to functionally identified alpha-, beta- and delta-cells in intact mouse pancreatic islets. The maximum rate of capacitance increase in beta-cells during a depolarization to 0 mV was equivalent to 14 granules s(-1), <5% of that observed in isolated beta-cells. Beta-cell secretion exhibited bell-shaped voltage dependence and peaked at +20 mV. At physiological membrane potentials (up to approximately -20 mV) the maximum rate of release was approximately 4 granules s(-1). Both exocytosis (measured by capacitance measurements) and insulin release (detected by radioimmunoassay) were strongly inhibited by the L-type Ca(2+) channel blocker nifedipine (25 microm) but only marginally (<20%) affected by the R-type Ca(2+) channel blocker SNX482 (100 nm). Exocytosis in the glucagon-producing alpha-cells peaked at +20 mV. The capacitance increases elicited by pulses to 0 mV exhibited biphasic kinetics and consisted of an initial transient (150 granules s(-1)) and a sustained late component (30 granules s(-1)). Whereas addition of the N-type Ca(2+) channel blocker omega-conotoxin GVIA (0.1 microm) inhibited glucagon secretion measured in the presence of 1 mm glucose to the same extent as an elevation of glucose to 20 mm, the L-type Ca(2+) channel blocker nifedipine (25 microm) had no effect. Thus, glucagon release during hyperglycaemic conditions depends principally on Ca(2+)-influx through N-type rather than L-type Ca(2+) channels. Exocytosis in the somatostatin-secreting delta-cells likewise exhibited two kinetically separable phases of capacitance increase and consisted of an early rapid (600 granules s(-1)) component followed by a sustained slower (60 granules s(-1)) component. We conclude that (1) capacitance measurements in intact pancreatic islets are feasible; (2) exocytosis measured in beta-cells in situ is significantly slower than that of isolated cells; and (3) the different types of islet cells exhibit distinct exocytotic features.

Action Potentials↗

PubMed2004

Reconstructing chromosphere concentration images directly by continuous-wave diffuse optical tomography.

We present an algorithm to reconstruct chromosphere concentration images directly rather than following the traditional two-step process of reconstructing wavelength-dependent absorption coefficient images and then calculating chromosphere concentration images. This procedure imposes prior spectral information into the image reconstruction that results in a dramatic improvement in the image contrast-to-noise ratio of better than 100%. We demonstrate this improvement with simulations and a dynamic blood phantom experiment.

Algorithms↗

PubMed2004

[Clinical research of sentinel node biopsy in oral tongue carcinoma].

OBJECTIVE: This study was designed to evaluate whether sentinel node (SN) biopsy can accurately assess the cervical lymph node status of oral tongue carcinoma, as well to research the best method and indications of SN biopsy. METHODS: Preoperative lymphoscintigraphy with (99m)Tc-SC and intraoperative sentinel node mapping with methylene blue dye were administered on 20 cases of oral tongue carcinoma with cN(0) neck and 5 cases with cN(+) neck; routine pathological examination was used to assess the status of SNs. The results of routine pathological examination of cervical specimen were set as golden standard to assess the efficacy of SN biopsy in evaluating the cervical lymph node status. RESULTS: 53 SNs were detected in 24 cases out of the total 25 cases (96%), averaging 2.2 SNs per case. SNs were detected in all 20 cases with cN(0) neck, in which 4 cases with occult cervical metastasis were detected by SN diopsy, without false negative case found in the procedure. In 5 cases with cN(+) neck, SNs were detected in 4 cases. In 4 cases whose SNs were detected, there were 5 cN(+) necks, out of which SNs were detected in 4 cN(+) necks but failed to predicted the cervical lymph node status in 2 necks. However, SNs were detected in 2 out of the other 3 cN(0) necks, both of which were diagnosed as SN(+)pN(+). CONCLUSIONS: Nuclear lymphoscintigraphy and blue dye mapping can be used to trace the SNs in cases with oral tongue carcinoma, with satisfactory detective rate. SN biopsy can accurately evaluate the cervical lymph node status in cases of oral tongue carcinoma with cN(0) neck. Whether it can be used to evaluate the lymph node status of the cN(0) neck in case with a contralateral cN(+) neck is worthy of further research.

Humans↗

PubMed2004

[Study on the attenuation of graft versus host disease by methoxy polyethylene glycol modification of donor lymphocytes].

AIM: To find out why mPEG modification of donor's lymphocytes can attenuate the occurrence of graft versus host disease(GVHD), but not affect the hemopoietic reconstitution of stem/progenitor cells after transplanting the mPEG-modified mononuclear cells from human cord blood into the SCID mice. METHODS: The followings were observed: (1) Changes of CD4(+) and CD8(+) T cells and the ratio of CD4(+)/CD8(+) T cells were examined by flow cytometry before and after mononuclear cells from human cord blood were modified with mPEG. (2) The difference in forming the CFU-GM in-vitro between the mPEG modified-stem/progenitor cell group and non-modified cell group was observed. (3) The time of appearance of GVHD and the survival of the SCID mice were observed after the pre- and post-modification mononuclear cells were transplanted. (4) The number of humanized CD45(+) cells in the mouse's bone marrow was detected about 7 weeks after transplantation. RESULTS: (1) mPEG nearly completely covered up the CD4 and CD8 antigens on T cells, while the number of CFU-GM did not show any obvious change between the modified and non-modified cell groups. (2) GVHD appeared later in the modified mononuclear cell group than in the non-modified group, and the survival rate was elevated in the modified group than in the non-modified group. (3) Humanized CD45 cells were found in mouse's bone marrow at the 47th day after transplantation of both mPEG-modified and non-modified mononuclear cells. CONCLUSION: After CD4 and CD8 antigens were covered up with mPEG, the graft's immune response against host was weakened, but the proliferation and differentiation of transplanted hemopoietic stem/progenitor cells were not affected.

Animals↗

PubMed2004

[Clinical evaluation of management of superior mediastinal metastasis from thyroid carcinoma with systemic superior mediastinal dissection via sternotomy approach: 12 cases report].

BACKGROUND & OBJECTIVE: Few research of surgery for superior mediastinal metastasis from thyroid carcinoma has been reported. Previous surgical approach from neck had the problems of easy damage of nerve and vessels and the difficulty of clearance of lymph nodes. This study was designed to assess the value of systemic superior mediastinal lymph node dissection via sternotomy for superior mediastinal metastasis from differentiated thyroid carcinoma. METHOD: A retrospective study was performed to analyze the clinical pathologic data as well as the outcome of 12 cases of differentiated thyroid carcinoma treated with systemic superior mediastinal lymph node dissection via sternotomy approach from April 1995 to April 2002, including 7 cases of papillary adenocarcinoma and 5 cases of medullary carcinoma. RESULTS: Pathologically, the incidence rates of metastasis to anteriotracheal lymph node, paratracheal lymph node, anterior superior vena cava, and anterior innominate artery lymph nodes were 55.6% (25/45), 57.1% (36/63), 42.1% (8/19) and 30% (3/10), respectively. All cases are still alive in one to seven years follow-up period. The median follow-up was 32 months. Upper mediastinal lymph nodes were recurrent in one case; distant metastasis occurred in two cases. CONCLUSION: Systemic superior mediastinal lymph node dissection via sternotomy approach can be employed in treating superior mediastinal lymph node metastasis from differentiated thyroid carcinoma due to its safety and relatively satisfactory outcome.

Adenocarcinoma, Papillary↗

PubMed2004

[Surgical treatment of differentiated thyroid carcinoma and analysis of recurrence-related factors].

BACKGROUND & OBJECTIVE: There are many controversies concerning the treatment to patients with differentiated thyroid carcinoma (DTC), focus on appropriate resection extent of DTC, indications for cervical lymph node dissection, et al. This study was to investigate surgical treatment for DTC, and recurrence-related factors. METHODS: Records of 581 patients with DTC treated at Cancer Center, Sun Yat-sen University from Jan. 1st, 1985 to Dec. 31st, 1997 were reviewed retrospectively. Various operation patterns for DTC and their treatment effects, and recurrence-related factors were analyzed. RESULTS: Among 377 patients with DTC who received initial surgery at our hospital, and whose main operation patterns were unilateral lobectomy plus isthmectomy with or without cervical lymph node dissection, 28 (7.43%) recurred, the rate of recurrence in contralateral lobe was 0.80%. Among 195 patients with DTC who received initial surgery in other hospitals, and whose main operation patterns were local mass excision with or without cervical lymph node resection, 143 patients received operation again in our hospital, and 100 of 143 (69.93%) were pathologic positive, positive rate of thyroid was 46.15%, that of cervical lymph node was 48.95%; 47 of 195 patients (24.10%) recurred. Recurrence rate of patients mainly received local mass excision with or without cervical lymph node resection was higher than that of patients mainly received unilateral lobectomy plus isthmectomy with or without cervical lymph node dissection (P >0.05). CONCLUSIONS: Unilateral lobectomy plus isthmectomy with or without cervical lymph node dissection should be recommended as the first treatment for DTC with primary focus localized in unilateral lobe. The initial operation pattern may influence recurrence of DTC.

Adenocarcinoma, Follicular↗

PubMed2004

[Survivin expression in laryngeal squamous cell carcinoma and its relationship with clinical factors].

BACKGROUND & OBJECTIVE: Survivin, a new described member of the family of apoptosis inhibitory proteins (IAP), is undetectable in normal tissue of adults, but abundantly expressed in a variety of human malignancies. This study was designed to examine the expression of Survivin in laryngeal squamous cell carcinoma, analyze the relationships between the expression of Survivin and prognostic factors including age, gender, clinical stage, T stage, N stage, classification, histology and recurrence, to investigate its significance on prognosis. METHODS: Clinical information and paraffin- embedded tumor samples of 81 cases laryngeal squamous cell carcinoma (LSCC) patients, who were treated at Cancer Center of Sun Yat-sen University between 1997 and 1998, were collected. Tissue chip was made, and SP method was performed to analyze Survivin expression in tumor tissue and adjacent normal laryngeal mucosa. SPSS 10.0 software was used. Kaplan-Meier method was used for survival analysis, and log-rank test was used for data analysis; multivariate analysis was carried out using Cox proportional hazard model. RESULTS: The overall 5-year survival rate for patients with laryngeal squamous cell carcinoma in this study was 58.33%. The over-expression rate of Survivin in tumor tissue and adjacent normal laryngeal tissue were 50.62%, 0%, respectively (P< 0.05). The positive Survivin expression group(5-year overall surivial rate: 22.06%) had a worse prognosis than that of negative group (5- year overall survival rate: 92.33%)(P< 0.05). The univariate analysis showed that clinical stage, T stage, N stage, histology grade, recurrence, and Survivin expression were the most important prognostic factors (P< 0.05). The Chi(2) test results showed that the expression of survivin was correlated with clinical stage, T stage, N stage, histology grade and recurrence (P< 0.05); Multivariate analysis results showed that clinical stage, survivin expression, and recurrence were independent prognosis factors on survival in LSCC patients. CONCLUSIONS: The expression of Survivin is an important biological factor affecting the prognosis; Tissue chip method can be simply and efficiently used in large scale of retrospective study in laryngeal squamous cell carcinoma.

Carcinoma, Squamous Cell↗

PubMed2004

[Surgical treatment of well-differentiated thyroid carcinoma invading trachea: a report of 15 cases].

BACKGROUND & OBJECTIVES: At present head and neck surgeons from many countries have different opinions on management of well-differentiated thyroid cancer (WDTC). We will discuss WDTC invading trachea surgical treatment and its clinical significance. METHODS: Retrospectively reviewed clinical data of 15 cases WDTC invading trachea, According to WDTC invading extent and grade, there were 3 kinds of surgical approaches: 1) end to end anastomosis; 2) tissue flap reconstruction; 3)larynx-tracheal dissociation. RESULTS: 15 cases underwent radical resection and reconstruct the defect of tracheal or larynx-tracheal dissociation. 2 cases received directly suture, 5 cases received sternocleidomastoid muscle flap reconstruction, 2 cases received pectorlis major muscle flap reconstruction, 2 cases received platysma flap reconstruction, 2 cases received free forearm flap with muticore titanium-board reconstruction, 2 cases received larynx-tracheal dissociation with larynx block out and tracheal fistula. 10 cases (10/15, 66.7%) received decannulation postoperation. Patients who were success fully decannulated could recover phonation and maintain airway breath. In the 5 patients who couldn't decannulate, underwent sternocleidomastoid muscle flap reconstruction, 1 underwent free forearm flap with muticore titanium-board reconstruction, 1 underwent pectorlis major muscle flap reconstruction, 2 underwent larynx-tracheal dissociation, but all of them could hardly utter voice by compress tracheostoma and needed permanent tracheostoma due to collapse of trachea. The recurrence rate of our group is 33.33%, 5 years survival rate is 88.89%. CONCLUSIONS: WDTC with trachea invading easily cause dyspnea or emptysis influencing on 5 years survival rate. We should take more actively surgical approach to resect all the tumor and involved organ, thus improve survival rate and reduce recurrence postoperation.

Adenocarcinoma, Follicular↗

PubMed2004

[Distribution of hepatitis B virus genotype among population of Dong, Miao minority and Han in Guizhou].

OBJECTIVE: To study the distribution of hepatitis B virus (HBV) genotype in population of Dong, Miao minority and Han in Guizhou. METHODS: S region nucleotides were compared in 127 strains whole sequence of HBV and three restriction enzymes which can be used for genotyping were found by DNA software analysis system. The partial gene fragment of HBV S region was amplified by nested polymerase chain reaction (nPCR). The products were digested with Mbo I, BstN I or BsmA I and subjected to electrophoresis on agarose gel, respectively. The patterns of restriction fragment length polymorphism (RFLP) were analysed. The genotypes were determined by nPCR-RFLP in 166 asymptomatic HBV carriers (ASC), including 48 Dong minority, 52 Miao minority and 66 Han subjects. Some of the ASC were also analysed by direct sequencing of PCR products. RESULTS: The nPCR-RFLP method was simple and accurate. Of the 166 ASC, 138 (83.13%) were genotype B, and 28 (16.87%) were genotype C. Of the 48 Dong minority subjects, 47 (97.2%) were genotype B and 1 (2.08%) was type C. Of the 52 Miao minority subjects, 49 (94.23%) were genotype B and 3 (5.77%) were genotype C. Of the 66 Han subjects, 42 (63.64%) were genotype B and 24 (36.36%) were genotype C. There was a statistical significance in the distribution of genotype C between Dong, Miao minority and Han (7.85% vs 36.36% P less than 0.005). CONCLUSION: Genotype B and C exist in Guizhou and genotype B is the major genotype. Genotype C is found more frequently in Han than in Dong and Miao minority.

Adolescent↗

PubMed2003

Tomographic optical breast imaging guided by three-dimensional mammography.

We introduce a modified Tikhonov regularization method to include three-dimensional x-ray mammography as a prior in the diffuse optical tomography reconstruction. With simulations we show that the optical image reconstruction resolution and contrast are improved by implementing this x-ray-guided spatial constraint. We suggest an approach to find the optimal regularization parameters. The presented preliminary clinical result indicates the utility of the method.

Breast↗

PubMed2003

Fluorescence optical diffusion tomography.

A nonlinear, Bayesian optimization scheme is presented for reconstructing fluorescent yield and lifetime, the absorption coefficient, and the diffusion coefficient in turbid media, such as biological tissue. The method utilizes measurements at both the excitation and the emission wavelengths to reconstruct all unknown parameters. The effectiveness of the reconstruction algorithm is demonstrated by simulation and by application to experimental data from a tissue phantom containing the fluorescent agent Indocyanine Green.

Bayes Theorem↗

PubMed2003

Examination of the cytoplasmic DNA in male reproductive cells to determine the potential for cytoplasmic inheritance in 295 angiosperm species.

Mature pollen grains of 295 angiosperm species were screened by epifluorescence microscopy for a marker that denotes the mode of cytoplasmic inheritance. We used the DNA fluorochrome DAPI (4',6-diamidino-2-phenylindole) for pollen cell staining. The presence or absence of fluorescence of cytoplasmic DNA in the generative cell or sperm cells was examined in each species. The species examined represented 254 genera and 98 families, and 40 of these families had not been previously studied in this regard. The cytoplasmic DNA of the generative cell or sperm cells did not fluoresce in 81% of the species examined, from 83% of the genera and 87% of the families examined, indicating the potential for maternal cytoplasmic inheritance in these species. In contrast, the male reproductive cells of 19% of the species, from 17% of the genera and 26% of the families examined, displayed fluorescence of the cytoplasmic DNA, indicating the potential for biparental cytoplasmic inheritance in these species. The results revealed the potential for biparental cytoplasmic inheritance in several species in which the inheritance mode was previously unknown, including plants in the Bignoniaceae, Cornaceae, Cruciferae (Brassicaceae), Cyperaceae, Dipsacaceae, Hydrocharitaceae, Papaveraceae, Portulacaceae, Tiliaceae, Valerianaceae, and Zingiberaceae. Electron microscopy revealed that the sperm cells of Portulaca grandiflora contain both plastid and mitochondrial DNA. However, in the generative cells of Musella lasiocarpa, the mitochondria contain DNA, but the plastids do not. These data provide a foundation for further studies of cytoplasmic inheritance in angiosperms.

Cytoplasm↗

PubMed2003

[Clinical and pathological related factors of occult cervical lymph node metastasis in squamous cell carcinoma of tongue].

BACKGROUND & OBJECTIVE: Some clinical and histopathological features of squamous cell carcinoma of tongue (TSCC) were closely associated with occult cervical lymph node metastasis (OCLNM). This study was designed to investigate the correlation between occult cervical lymph node metastasis in squamous cell carcinoma of tongue and the clinicopathological indexes of primary tumor tissue. METHODS: Thirty-five TSCC patients with OCLNM treated in Cancer Center, Sun Yat-sen University from 1988 to 1996 were enrolled. According to random principle, 35 TSCC patients without OCLNM at the same period were selected. Tumor deepness was measured and pathological parameters were assessed under microscope. RESULTS: The rates of OCLNM in group of infiltrative plus ulcerative type, group of infiltrative type, group of ulcerative type and group of exogenous type were 70.37%, 41.67%, 40.00%, and 27.27%, respectively; the rates of OCLNM in T1, T2, and T4 were 44.00%, 43.48%, and 62.64%, respectively. The rates of OCLNM in groups of tumor deepness < 4 mm, 4-7.9 mm, and > or = 8 mm were 14.29%, 61.54%, and 88.89%, respectively. The rates of OCLNM in groups of response of peritumoral lymphocyte +, ++ and +++ were 73.68%, 58.62%, and 18.18%, respectively. The rates of OCLNM in groups of with vascular invasion and without vascular invasion were 85.71% and 46.03%, respectively. The rates of OCLNM in groups of well, moderate, and poor differentiation were 52.63%, 42.31%, and 66.67%, respectively. The rates of OCLNM in groups of invasive patterns of type I, type II, type III, and type IV were 40.90%, 50.00%, 52.00%, and 80.00%, respectively. Univariate analysis indicated that OCLNM in TSCC was closely associated with gross appearance of primary tumor, tumor deepness, response degree of peritumoral lymphocyte and vascular invasion (P < 0.05), but was not associated with T stage, pathological differentiation, and invasion way (P > 0.05); Logistic multivariate analysis manifested that only tumor deepness and response degree of peritumoral lymphocyte significantly correlated with OCLNM in TSCC. CONCLUSION: There was significant correlation between gross appearance of primary tumor, tumor deepness, response degree of peritumoral lymphocyte and vascular invasion and OCLNM in TSCC. These parameters can be used to predict occult cervical lymph node metastasis in TSCC.

Adult↗

PubMed2003

[Application of microwave coagulation in treatment of cancer of oral cavity: summary of 96 cases].

BACKGROUND & OBJECTIVE: There was few report about the protection of oral function of microwave coagulation in treatment of cancer of floor of the mouth. This study was designed to systematically clarify the principle, operation, treatment of the primary cancer and lymph nodes, efficiency, function of the mouth, complications and indications of the microwave coagulation in treatment of the cancer of the oral cavity. Based on this, the significance of the two special methods and prevention of bleeding during the cause were discussed. METHODS: Ninety-six cases with cancer of oral cavity who were treated with microwave coagulation were analyzed retrospectively. The survival rates and the recurrent rates of those with the coagulated tissue be resected or unresected, and of the positive margin group and the negative margin group were compared for analyzing the complications and prevention of them. RESULTS: The difference of the survival rates of those whose coagulated tissue pathologically positive and negative was insignificant (Log Rank = 0.70, P = 0.4033), and the differences of the recurrent rates of the primary site, secondary site and the uncertain site of them were also insignificant (chi 2 = 1.65, 0.837, 0.003; P = 0.684, 0.36, 0.959). The overall survival rate of the patients with negative margin was better than those of with positive margin (Log Rank = 6.08, P = 0.0136). However, the difference of the recurrent rates of them was insignificant (chi 2 = 0.327, P = 0.567). The total complication rate of 96 patients was 9.6% and all of them were acceptable. The complication rate of those with the coagulated tissue be resected was lower than that of the unresected (P = 0.013). CONCLUSION: Ligating the tongue artery, resecting the coagulated tissue, and packing the wound margin were effective methods to prevent the bleeding. The pathological result of the coagulated tissue could not be used to predict the prognosis. It is best to hold enough treatment range for microwave coagulation. Application of microwave coagulation in treatment of cancer of oral cavity has the advantage of less and slight complications.

Adult↗