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Biomedical subjects

Q Xie

Publications and source records attributed to Q Xie.

137 records · Page 8Linked to original sources
PubMed1991

[Transitional mucosa in carcinoma of large bowel. Mucin histochemical and scanning electron microscopical study].

We studied the mucosa close to the carcinoma of large bowel (CLB) in 200 cases, the carcinoma tissue of CLB in 130 and the mucosa of fetus large bowel in 24 by mucin histochemistry, in addition to the mucosa near CLB studied by scanning electron microscope (SEM) in 25. The results suggested that mucin composition of transitional mucosa (TM) was similar to CLB and large bowel mucosa of the fetus. SEM showed significant changes in TM. The HID/AB Stain suggesting abnormal range of TM was within 2 cm in 68.0% (136/200) of cases of CLB, within 3 cm in 88.5% (177/200) and In SEM abnormal range within 2 cm in 88.0% (22/25). The measurement of TM can provide reference for resectable range of CLB.

Colonic Neoplasms↗
PubMed1991

Experimental study on the corneal endothelium of traumatic cataract.

The cell morphology of corneal endothelium in 84 mice with experimental traumatic cataract was investigated with stained corneal buttons. In the experimental group, the boundaries between adjacent corneal endothelial cells were significantly distorted, some cell boundaries manifested degenerative changes that led to coalescence of the cells. The mean density and mean area of endothelial cells of the controls showed significant difference from those of the experimental group during the 12 weeks of observation. The density of endothelial cells decreased from 3,312 +/- 337/mm2 of the control group to 2,944 +/- 418/mm2 in the group of partially opaque lenses and 2,713 +/- 472/mm2 in the group of totally opaque lenses. Meanwhile, the area of endothelial cells and the coefficient of variation also significantly changed correspondingly, and the degree of damage in the corneal endothelium correlated with the degree of the lens opacity.

Animals↗
PubMed1991

[Dynamics of endothelial cells of the peripheral area of cornea].

Clinical corneal buttons of 150 cases were stained with try-pan blue and alizalin red S, so as to compare the relative density of endothelial cells and to analyse the forms of the cell nuclei. The result showed that cell densities of fetal corneal endothelium of 25 cases with fetal period from 19 to 34 weeks, had no significant difference (P > 0.05) between the central and peripheral areas of cornea. However, the cell densities of corneal endothelium of 19 cases, aged from newborn to 53 years, showed a significant difference (p < 0.01). The cell nuclei of the fetal corneal endothelium have elliptic forms mainly, which are similar between central and peripheral area of corneal endothelium. However, the forms show an unstable nucleus morphological structure. From newborn to adult cornea, the nucleus forms of corneal endothelial cells tend to be stable and circular, but there are unstable forms of endothelial cell nuclei dispersed in corneal periphery. This emphasize that the endothelial cells in corneal periphery still keep its cell differential function of development in some degree.

Adolescent↗
PubMed1987

Epitopes on foot-and-mouth disease virus particles. I. Topology.

Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-sensitive sequential site, termed B2/D9, from the codings for the MAb which reacted with it, which was associated with virus infectivity and is probably at or near to the cell-binding site of the virion; a trypsin-resistant, conformational site 1C6/4C9 MAb reaction at which also resulted in neutralization of virus infectivity; a second trypsin-resistant, conformational site 3C8, where again MAb reaction neutralised virus infectivity; a third trypsin-resistant, conformational site 6C3/2G5, at which MAb-dependent neutralisation of virus infectivity was inefficient; a site 3G4, the expression of which was impaired but not destroyed by trypsin treatment, and was not related to virus infectivity; an internal site A8, which appears to be a "12S subunit-specific" site. This work clearly demonstrates for the first time that both trypsin-sensitive and trypsin-resistant neutralisable (infectivity-associated) sites exist on the FMDV particle, and only one of these can be related to the sequential site used to formulate current FMDV peptide vaccines.

Antibodies, Monoclonal↗
PubMedDate not supplied

Cloning and molecular analysis of two different ILV5 genes from a brewing strain of Saccharomyces cerevisiae.

Two different ILV5 genes encoding acetohydroxy-acid isomeroreductases, and named ILV5G and ILV5X, were cloned and sequenced from a Saccharomyces cerevisiae brewing strain. The coding sequence of ILV5X shows a single nucleotide change with respect to that from the ILV5 gene of a S. cerevisiae laboratory strain. In addition, all promoter motifs which are, or are presumed to be, implicated in transcription regulatory functions are identical in ILV5 and ILV5X. In contrast, the coding sequence of ILV5G differs in 5.6% of its nucleotides from that of ILV5 and most of its promoter regulatory motifs show a single nucleotide change with respect to those from ILV5.

2-Acetolactate Mutase↗
PubMedDate not supplied

Comparative molecular field analysis (CoMFA) model using a large diverse set of natural, synthetic and environmental chemicals for binding to the androgen receptor.

A large number of natural, synthetic and environmental chemicals are capable of disrupting the endocrine systems of experimental animals, wildlife and humans. These so-called endocrine disrupting chemicals (EDCs), some mimic the functions of the endogenous androgens, have become a concern to the public health. Androgens play an important role in many physiological processes, including the development and maintenance of male sexual characteristics. A common mechanism for androgen to produce both normal and adverse effects is binding to the androgen receptor (AR). In this study, we used Comparative Molecular Field Analysis (CoMFA), a three-dimensional quantitative structure-activity relationship (3D-QSAR) technique, to examine AR-ligand binding affinities. A CoMFA model with r2 = 0.902 and q2 = 0.571 was developed using a large training data set containing 146 structurally diverse natural, synthetic, and environmental chemicals with a 10(6)-fold range of relative binding affinity (RBA). By comparing the binding characteristics derived from the CoMFA contour map with these observed in a human AR crystal structure, we found that the steric and electrostatic properties encoded in this training data set are necessary and sufficient to describe the RBA of AR ligands. Finally, the CoMFA model was challenged with an external test data set; the predicted results were close to the actual values with average difference of 0.637 logRBA. This study demonstrates the utility of this CoMFA model for real-world use in predicting the AR binding affinities of structurally diverse chemicals over a wide RBA range.

Androgens↗
PubMedDate not supplied

Genital human papillomavirus testing by in situ hybridization in liquid atypical cytologic materials and follow-up biopsies.

OBJECTIVE: To describe cases of HPV testing by DNA in situ hybridization performed on atypical cervicovaginal samples collected by a liquidsed method that were negative for HPV DNA on cytology but revealed cervical intraepithelial neoplasia on follow-up biopsies. STUDY DESIGN: Three hundred ninety-five consecutive SurePath atypical squamous cells of undetermined significance (ASC-US) cytologic samples from asymptomatic, reproductive-age women were tested for human papillomaviruses (HPVs) by the in situ hybridization (ISH) method (Ventana Inform HPV Test, Tucson, Arizona, U.S.A). One hundred (25%) cases underwent follow-up colposcopic biopsy within 3 months of cytology. All the tests (cytology, ISH, histology) were independently evaluated without knowledge of the other tests. RESULTS: One hundred twenty-two (33%) cytologic samples were positive for HPVs. Of a total of 100 (HPV positive and negative) follow-up biopsies, 55 were positive for cervical intraepithelial neoplasia (CIN). Fourteen cases of biopsy-proven CIN tested negative for all HPV types in the prior cytologic samples. Retesting of the 14 CIN tissues by ISH was negative in 10, positive for HPV in 2 and inconclusive in 2. CONCLUSION: There is a small but significant (14%) false negative rate with HPV testing by the Ventana ISH method. Clinically suspicious cases should be followed even if an HPV test is negative.

Adolescent↗
PubMedDate not supplied

A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains.

Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM16 and HVT strains.

Animals↗
PubMedDate not supplied

Patterns of efferent lymphatics of the mouse testis.

Previous studies in the rat demonstrated that lymph vessels arise from various sites in the testis and then anastomose to form the main lymph trunk toward the testicular artery. Although mice are as commonly used in the laboratory as the rat, precise knowledge concerning their testicular lymphatics has not been established. In the present study, the patterns of testicular lymphatic drainage in the mouse were examined using local injection of India ink into the testicular parenchyma. The results demonstrated that, besides the lymph trunk surrounding the testicular artery, a lymphatic drainage route running along the ductus deferens is also present.

Animals↗