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Biomedical subjects

Q Luo

Publications and source records attributed to Q Luo.

At least 73 records · Page 4Linked to original sources
PubMed1998

[Esophageal reconstruction using ilecolon in children--a report of ten cases].

The use of the ileocolon as a substitute for esophagus in children has been a well-established surgical procedure. Between 1992 and 1995, ten retrosternal esophageal substitutions using an ileocolic interposition were performed. The ages of the children ranged from 2 to 8 years. The esophageal strictures were secondary to ingestion of caustic liquid. No death occurred in the intra- and post-operation period. Cervical ileoesophageal anastomotic leaks took place in 2 cases as the early major postoperative complications, which healed spontaneously. Three cases of ileoesophageal anastomotic strictures were noted in the follow-up period. The surgical indications, the treatments of anastomotic leaks and strictures, and the importance of long-term follow-up were discussed.

Burns, Chemical↗
PubMed1998

[The observation on infection of trasgenic mice (PVR Tg21) with polioviruses type 3].

OBJECTIVE: To test and verify whether PVR Tg21 transgenic mice can be used as an animal model or not. METHODS: PVR Tg21 transgenic mice were infected with 3 poliovirus strains with different neurovirulence intraspinally. RESULTS: All the mice showed clinical paralysis with characteristic lesions in neurons. The symptoms varied with different potency of virus. The onset of clinical paralysis time for inoculated mice were approximately 2 days with Leon virus, 5 days with Sabin strain, and 4 days with Vaccine-321. More than 90% of mice inoculated with Leon strain died within 3 days after clinical paralysis and only 35% mice inoculated with Sabin-3 died at average of 6 days, and the PD50 are 10(1.1), 10(2.6), and 10(4.97) TCID50 and LD50 are 10(1.1), 10(3.33) and 10(5.33) TCID50, respectively. Different virus inoculated intraspinally caused different histopathological lesions. CONCLUSIONS: The PVR Tg21 transgenic mice could be a new animal model for neurovirulent evaluation of poliovaccines and epidemiological surveillance.

Animals↗
PubMed1998

[Production of allochtonic formations in callus cells of Salvia miltiorrhiza Bge].

OBJECTIVE: To study the effect of hormones content in the medium on of allochtonic formations in the callus from Salvia miltiorrhiza. METHOD: Study the development of allochtonic formations in the callus from explants of Salvia miltiorrhiza cultured on hormones of different content ratios in the medium. RESULT AND CONCLUSION: 2,4-D inhibits the production of water-soluble active principles and KT promotes the production of total tanshinone.

Abietanes↗
PubMed1998

[Cloning and sequencing of fusion protein gene of recombinant GM-HBsAg].

GM-CSF is an effective adjuvant for immunology, it was used to improve the immunological function of antigen and to increase the anti-HBs responses. Through using polymerase chain reaction (PCR) technique, we amplified 384 bp and 846 bp gene fragments from GM-CSF and HBsAg. The products were extracted and purified; a 1230 bp gene fragment was obtained by T4 DNA linkage. This amplified product was cloned into pUC19 vector. The positive clones were screened and the inserted fragment was determined by restriction enzymes. The gene fragment length determined is 1230 bp, the sequence analysis showed that the gene fragment is GM-CSF-HBsAg gene. The successful construction of GM-HBsAg fusion protein gene provides basis for research of its biological activity and expression.

Base Sequence↗
PubMed1998

[The real-time classification of motor unit potentials].

Motor unit potentials(MUP) are real-time classified with the method of correlation in this paper. The correlation coefficients are sorted to reserve the better waves of MUP. Not only the store waves and analytical time of MUP can be reduced, but also the measurement correction and precision of MUP parameters can be improved.

Action Potentials↗
PubMed1997

Optical investigations of physiology: a study of intrinsic and extrinsic biomedical contrast.

The utility and performance of optical studies of tissue depends upon the contrast and the changes of contrast in health and disease and in functional activity. The contrast is determined both by the optical properties of extrinsic chromophores and scatterers but especially upon the changes evoked by physiological activity and pathological states. Here, we have focused upon absorption changes of the intrinsic probe, blood absorbance changes due to cortical hypoxia and to haematomas, giving, for particular conditions, absorbance changes of 0.15 and over 0.4 delta OD, respectively. Functional activity may give changes of blood volume of over 0.05 delta OD with some variability due to individual responses that is best expressed as histogram displays of the distribution of response among a significant population. Responses have been observed in prefrontal parietal and occipital functions (242 tests). Extrinsic probes afford signals dependent upon the dose tolerance of the subject and can readily equal or exceed the blood volume and oxygenation signals, and currently afford vascular volume and flow indications. However, contrast agents for the functional activity of cellular function are ultimately to be expected. Finally, light-scattering changes afford osmolyte-related responses and are here shown to indicate a larger signal attributed to cortical depolarization and K+ release in hypoxia/ischaemia. Thus, the optical method affords imaging of manifold contrasts that greatly enhance its specificity and sensitivity for diagnostic procedures.

Animals↗
PubMed1997

Human brain functional imaging with reflectance CWS.

Continuous Wave Spectroscopy (CWS) has been used in human subjects to detect brain function by observation of brain oxygenation and blood volume changes. In this research, we have developed a device for imaging brain oxygenation and blood volume with multiple sources and detectors in an area of 10 x 12 cm. Twelve, 1 watt tungsten bulbs were used as a light source, together with 8 detectors consisting of silicon-photodiodes with band-pass filters at 760 and 850 nms. The detectors were placed on a model system of the human head, providing 16 source-detector combinations with 4 cm separation. Eight to 16 seconds were required to image a brain function. Models using resin and a black object which have similar optical characteristics to a brain were studied. The model study showed that we can detect changes in absorption as deep as 2 cm from the surface. Human subjects with relatively thin hair, who volunteered for this study, were asked to tap fingers and rest, or to observe light flashing and darkness alternatively for 10 to 20 minutes while the images were being taken. In the finger tapping, the blood volume increased in a small, 1-2 cm area, while in the light stimulation, a much larger area was affected with a greater increased blood volume. Oxygenation occurred also in the area where blood volume increased. We concluded that the CWS can be used to image the location of brain activity by means of blood volume increase and oxygenation. In addition, brain disfunction and diseases, such as learning disability and brain hematoma may be detected with this imaging device.

Adolescent↗
PubMed1997

[Detection of mRNA expression of endothelin-1 in organs on severe burn rat model with in situ hybridization technique].

After the dynamic observation of postburn endothelin changes in plasma on severe burn rat model, we detected ET-1 mRNA expression and cell localization in some organs such as heart, lung, liver and kidney with in situ hybridization technique. We that endothelin concentration in plasma rapidly increased postburn (it reached its climax at 6th hour and maintained fairly high level even at 24th hour postburn). Meanwhile in situ hybridization indicated that ET-1 mRNA expression intensity and number of positive cells rapidly increased postburn. The expression climaxes of which appeared decreasing in turn from kidney, liver, lung to heart and the types of positive cells increased. This may due to the endothelin's functions of contracting blood vessels and regulating the redistribution of blood flow and be helpful for guaranteeing blood perfusion of important organs under shock state, but the overexpression of ET-1 mRNA may make the ischemic and anoxic damages to organs more severe.

Animals↗
PubMed1997

[Effect of light colours and illumination intensity on the sexual behaviour of Hepialus gonggaensis Fu et Huang].

The sexual behaviour of Hepialus gonggaensis has close relations with ecological environment and varies with its ecotypes. The more intense the illumination, the faster the sexual drive of Hepialus gonggaensis begins and the longer its sexual intercourse continues, light colours have a bearing on its sexual intercourse time. This study has provided an evidence for the artificial cultivation of Hepialus gonggaensis.

Animals↗
PubMed1997

[The technology of real time telemetring and monitoring multi-channels ECGs through public telephone network].

In this essay a kind of technology of real time telemetring and monitoring multi channels ECGs through public telephone network is presented. Two limb leaders (I and II) ECG signals are transmitted from the sender at first. After they are received by the receiver the other four limb leaders are calculated by computer in the basis of the relation among the limb leaders, the received waveform and the calculated waveform are just the same as those described directly by Electro Cardiograph. After the limb leaders ECG signals (34 s) are transmitted, two selectable thorax leaders ECG signals are transmitted for 34 seconds after automatically changing the leaders. The received ECG waves are displayed on screen real time, shifting smoothly. After transmission doctors may make diagnoses and give some suggestion for treatment. Thus, telemetrying and monitoring multi channels ECGs is realized.

Electrocardiography↗
PubMed1997

[The development of ECG trans-telephone popular monitoring system].

In this essay a new kind of ECG telemetry and monitoring system based on public telephone network is presented, which is able to transfer four channels of ECG of two patients to monitoring center sited at hospital synchronizingly. At the same time doctors may make diagnosis and give instruction for treatment. The system has the functions of real time sample, reviewing, freezing, store, windows, and printing, etc.

Electrocardiography↗
PubMed1997

[Expression of ferritin receptor on placental microvillous membrane and its role in maternal-fetal iron transport].

OBJECTIVE: In order to investigate the expression of ferritin receptor (FnR) on human placental microvillous membrane in mid-pregnancy and its implication. METHODS: Radioligand binding assay was used, with horse spleen 125I-apo-ferritin as radioligand. RESULTS: The FnR expression in normal placental microvillous membrane in mid-pregnancy was (9.63 +/- 4.72) x 10(12) sites/mg pro, similar to that in normal placental of term delivery,and the dissociation constants (Kd) between them had also no significant difference: Competitive binding assay indicated that the receptor bound basic ferritin with higher affinity than acidic one. CONCLUSIONS: The expression of FnR in mid-pregnancy almost come to mature level; The FnR on placenta might be involved in maternal-fetal iron transport, transfering iron from mother to fetal.

Apoferritins↗
PubMed1997

[Measurement and analysis of tumor tissue autofluorescence spectra].

Measurement results of laser induced autofluorescence spectra of tumor tissue from little rat are given in this paper it is compared with normal tissue of the same body After analyzing the origin of tumor tissue autofluorescence and the difference with normal tissue spectra, it is concluded that autofluorescence spectra of tissue may reflect the features of tumor tissue and be utilized to diagnose the tumor.

Animals↗
PubMed1996

Effect of a somatostatin analog (octreotide acetate) on the growth of retinal pigment epithelial cells in culture.

PURPOSE: Proliferative vitreoretinopathy is a serious complication of retinal detachment surgery in which retinal pigment epithelial cells abnormally proliferate within the vitreous cavity and under the retinal surface. Octreotide acetate, a somatostatin analog, has been shown to inhibit the cellular proliferation of a variety of cell types. The aim of this study was to investigate the effect of octreotide acetate on the growth of rabbit retinal pigment epithelial cells in culture. METHODS: Retinal pigment epithelial cells were isolated from rabbits and maintained in culture. Cells were exposed to standard media or media supplemented with octreotide acetate 10(-4) M to 10(-12) M for five days. Each concentration of octreotide acetate was tested in quadruplicate. RESULTS: Exposure of rabbit retinal pigment epithelial cells to octreotide acetate significantly inhibited proliferation with a peak effect at 10(-8) M. The effect of octreotide is biphasic with higher and lower concentrations having less effect than 10(-8) M. CONCLUSIONS: This study suggests that octreotide acetate may be useful in the treatment of proliferative vitreoretinopathy; however, the optimum therapeutic dose range for this drug may be narrow.

Animals↗
PubMed1996

[Clinical study of scopolamine detoxification for the treatment of heroin addicts].

OBJECTIVE: To evaluate the efficacy of treatment of heroin addicts (n = 100) by scopolamine detoxification (10 days program). METHODS: Methadone detoxification (10 days program) group (n = 50) and clonidine treated group (n = 50) served as controls. RESULTS: The scores of abstinence syndrome in scopolamine detoxification group were lower that those in clonidine treated group in the first three days of protocol, but this difference disappeared in the late stage of treatment. While scopolamine detoxification was effective as methadone detoxification in the control of abstinence syndrome during the first five days of treatment but the difference in the scores of abstinence syndrome between scopolamine and methadone group was observed during the late five days of protocol. The side-effects produced by scopolamine in general were dry mouth, somnolence, tachycardia, blurred vision and so on, which relieved gradually or disappeared with decreasing of its doses. CONCLUSION: Scopolamine does not result in potential dependence and has definite curative effect in the treatment of heroin addiction.

Adult↗
PubMed1996

Intravitreal tolerance of a new perfluorocarbon vitreous replacement, Multifluor APF-144.

OBJECTIVE: To evaluate the intravitreal tolerance of a new perfluorocarbon vitreous replacement, Multifluor APF-144 (perfluorotetramethylcyclohexane). DESIGN: Ten New Zealand albino rabbits (one eye from each) underwent vitrectomy. The vitreous was replaced in five eyes with Multifluor APF-144 and in five eyes with saline (control group). OUTCOME MEASURES: Appearance on indirect ophthalmoscopy, electroretinography recordings before and 2, 4 and 8 weeks after vitrectomy, findings on electron and light microscopy at 8 weeks. RESULTS: Endophthalmitis did not develop in any of the eyes. There was no significant change in electroretinography values for the experimental eyes after vitrectomy. No evidence of retinal toxicity was found on light or electron microscopic examination. CONCLUSIONS: Multifluor APF-144 shows promise as a short-term postoperative retinal tamponading agent.

Animals↗
PubMed1996

[The effects of intensive DMPA regimen in the treatment of Graves' ophthalmopathy].

35 patients with Graves' ophthalmopathy were treated with DMPA regimen which included dexamethasone (D) 20-30 mg and Methotrexate (M) 15-20 mg i.v. once a week and prednisone (P) 20 mg/d p. o. 4 times a week for 4 weeks followed by addition of azathioprine (A) or 6-MP 75-100 mg/d and the increment of prednisone (20 mg/d) to 5 times a week in the fifth and sixth weeks. The full intensive course of therapy lasted for 6 weeks. If necessary, the similar course of therapy might be repeated. Later on, each patient was mintainted on a therapy with P and A or P and 6-MP for another 1-2 months then the P and A or P and 6-MP for another 1-2 months, and then the P and A or 6-MP were tapered and finally discontinued at the end of sixth month. The symptoms and signs of the opthalmopathy were evaluated blindedly at the beginning and the end of the first and second courses of the therapy by two ophthalmologists. The results showed that the symptoms and signs were significantly improved in 33 (94.3%) cases. A decrease of eyeball protrusion of 1.22 +/- 1.15 and 1.28 +/- 1.15 mm in the left and right eyes respectively was shown by eophthalmometry reading (both P < 0.0005, compared with before and after the therapy). The decrease of exophthalmos was more remarkable in the cases with ophthalmopathy for < or = 1 year than that > 1 year. 11/22 patients recovered their ability to close eyes adequately. The diplopia was corrected in 8/15 cases. The mobility of eye ball returned to normal in 5/15 cases. Exposed lesion and/or ulceration of the cornea found in 7 patients were cured. Serious side and/or toxic effects of the drugs were not present during the course of therapy. It is suggested that DMPA regimen is an effective, safe and cheap approach to the treatment of Graves' ophthalmopathy.

Adult↗