Search PubMed⌕ Search

Biomedical subjects

Q Luo

Publications and source records attributed to Q Luo.

At least 55 records · Page 3Linked to original sources
PubMed2000

[Endoscopy-assisted neurosurgery for intracranial operation].

OBJECTIVE: To investigate the value of endoscope-assisted neurosurgery for intracranial operation. METHODS: 37 patients with intracranial lesions including tumors, aneurysms, cysts, hematomas, hydrocephalus and nerve-vessel compression syndrome were treated with this method. Their results were compared to those of microsurgery and macrosurgery. The items analyzed included exposure and protection of brain tissue and cranial nerves, views of operation field, length of procedure etc. RESULTS: 15 patients with tumors, showed a high rate of complete removal but less complications. The 6th nerves of CPA tumors operation were preserved. Six cases of nerve-vessel compression syndrome showed excellent therapeutic effects. In 8 cases of intraventricle lesions, using endoscope easily found tumor location and the cause of hydrocephalus. As to saccular diseases, endoscope was used to deal with the major location. In 4 cases of hematomas, less blot remained and the procedure shortened. Moreover the operation field was more clear and adequate on the expenses of no more complexity of the procedure. CONCLUSIONS: Endoscope-assisted microsurgery is helpful in intracranial operation. It might become a standard neuro-operation in near future.

Adolescent↗
PubMed2000

[Ultrastructure study on extraocular muscle of infiltrative exophthalmos].

OBJECTIVE: To investigate histopathologic and ultrastructural changes of extraocular muscles (EOMs) in infiltrative exophthalmos in order to collect some evidences of pathogenesis. METHODS: Ten EOM samples in infiltrative exophthalmos were obtained after operation. Each of them was cut into two pieces. One of them was stained with HE and observed by light microscope. Another one was sectioned with ultrathin method and observed by transmission electron microscope. RESULTS: Under the light microscope, the extraocular muscular cells had no significant abnormality at the early stage, while at the middle and late stage, sarcoplasm coagulation, granular degeneration, vacuolization and necrosis were found in the cells. Under the electron microscope, there were disturbance and disappearance of the Z line in part of muscular fibers in early cases; at the middle and late stage, various degrees of sarcoplasmic reticulum dilatation, myofilament lysis and destruction with formation of vacuoles, secondary lysosome and remnant bodies were seen. In severe cases, the muscular cells could be completely destroyed and phagocytosed by macrophages, fibrosis occurred and myofibroblasts were found in some cases. CONCLUSIONS: The EOMs in infiltrative exophthalmos are destroyed in various degrees, and the muscular cells may be the target cells in Graves' ophthalmopathy.

Adult↗
PubMed2000

[Treatment of severe inhalation injury with endotracheal infusion of exogenous pulmonary surfactant].

OBJECTIVE: To explore the possibility and the effects of clinical application of exogenous pulmonary surfactant (PS). METHODS: Two burned patients with severe inhalation injury were treated with 5% emulsified porcine PS by endotracheal infusion of 10 ml every 2 hours with the assistance of mechanical ventilation. The indices of patients' oxygenation and related pulmonary dynamics were observed. RESULTS: Twenty-four hours after the application of exogenous PS, PaO(2) and PaO(2)/FiO(2) increased evidently, but (A -- a) DO(2) and RI decreased obviously and PaCO(2) sustained for the observing period. The index related pulmonary dynamics, DeltaV/DeltaP increased gradually. CONCLUSION: With the assistance of mechanical ventilation, endotracheal infusion of exogenous PS could improve oxygenation and compliance of patients with severe inhalation injury. And the patients exhibited very good tolerance to exogenous PS.

Adult↗
PubMed2000

[The inhibitory effects of chloroquine and dexamethasone on the formation of pulmonary edema in the early stage of smoke inhalation injury in rats].

OBJECTIVE: To explore the inhibitory effects of chloroquine and dexamethasone on the formation of pulmonary edema in the early stage of smoke inhalation injury in rats. METHODS: Wistar rats were used and randomly divided into 10 groups, i.e. normal control, 1 PBH (postburn hour), 3 PBH, 6 PBH, 12 PBH and 24 PBH groups of smoke inhalation injury, 6 PBH and 12 PBH groups with dexamethasone treatment, and 6 PBH and 12 PBH groups with chloroquine treatment. The lung water content (LWC), pulmonary vascular permeability (PVP) and hemorrhagic amount (PHA) were measured. RESULTS: The LWC and PVP (measured by the pulmonary exudation amount of (131)I -- labelled albumin) increased obviously, peaked at 6 PBH and lasted to 24 PBH (P < 0.01) in rats injured by smoke inhalation. In groups treated with chloroquine or dexamethasone, the LWC and PVP were significantly lower at 6 PBH (P < 0.05). Similarly, the PHA in injured group exhibited remarkable, especially at 1 PBH and up to 24 PBH when the value was still higher than that in normal control group (P < 0.01). In groups treated with chloroquine or dexamethasone, PHA abated evidently at 6 PBH (P < 0.01). But there were no significant difference of the indices among groups at 12 PBH. CONCLUSION: Both chloroquine and dexamethasone be effective in preventing and treating pulmonary edema in rats in the early stage of smoke inhalation injury.

Animals↗
PubMed2000

[Study of graft-versus -leukemia activity of gamma-ray irradiated haplomismatched donor lymphocytes infusion].

OBJECTIVE: To explore graft-versus-leukemia activity of 7.5 Gy gamma-ray irradiated haplomismatched donor lymphocytes infusion (DLI). METHODS: L615 cells loaded 615 (H2(k))/BALB/c (H-2(d)) F(1) mice were infused with irradiated haplo-mismatched donor lymphocytes at 3, 5, 7 days after CTX (200 mg/kg) treatment which was given at the 3rd day after L615 cells inoculation. RESULTS: T-cells irradiated with 7.5 Gy gamma-ray lost their proliferative capacity but preserved their cytotoxic activity against allogeneic targets. Survival of these recipient mice was longer than that of control (t = 10.03, P < 0.001) with no severe graft-versus-host disease (GVHD). CONCLUSION: 7.5 Gy gamma irradiation attenuated the GVHD inducing potential of allogeneic lymphocytes while preserving their graft-versus-leukemia activity. It provides a simple modality to postpone and prevent leukemia relapse.

Animals↗
PubMed2000

[Effect of intravascular low level laser irradiation used in avulsion injury].

OBJECTIVE: To explore the effect of intravascular low level He-Ne laser irradiation on skin flap survival after orthotopic transplantation in avulsion injury. METHODS: Fifty eight cases suffered avulsion injury were treated by debridement and orthotopic transplantation of avulsed flap within 6 hours, 31 of them were received intravascular low level He-Ne laser irradiation and routine treatment, and 27 of them were received routine treatment as control group. RESULTS: The survival area and quality of avulsed flap in the experimental group were superior to that of control group after 15 days of operation, and the hemorheological items were markedly changed at 5 days after operation. CONCLUSION: The better flap survival after orthotopic transplantation in avulsion injury can be improved by intravascular low level He-Ne laser irradiation through changed superoxide dismutase activity and hemorheological items in optimal irradiation intensity.

Accidents, Traffic↗
PubMed1999

Structure-based ligand design by dynamically assembling molecular building blocks at binding site.

A structure-based ligand design method is proposed and tested. The method is based on stochastic dynamics simulation of multiple copies of molecular building blocks in the presence of a receptor molecule. The molecular building blocks are assembled into candidate compounds "on the fly" at given intervals during the simulation. In the algorithm, a special effort is made to explore different possible combinations of building blocks and to select an optimum combination. By repeating the cycle of deconstruction and reconstruction in a single simulation, a set of candidate compounds that can be built from the building blocks evolves and is dynamically optimized. The method was tested by breaking two known flexible human immunodeficiency virus type 1 protease inhibitors into building blocks and reassembling them in the active site of the enzyme. For the inhibitor L700417, a set of conformations was generated by the calculation. Among these, the original compound was recovered with the lowest energy at the experimentally observed binding site and in the correct conformation. For pepstatin, the experimentally observed binding mode of the backbone of the inhibitor was reproduced by a calculation in which the building blocks corresponding to the side-chain groups were omitted. Proteins 1999;36:462-470.

Algorithms↗
PubMed1999

Time-dependent changes in dough color in hexaploid wheat landraces differing in polyphenol oxidase activity.

Time-dependent changes in the color of noodle sheets (using 2% NaCl or 1% alkaline salts in the formulation) made from 43 Iranian hexaploid wheat landrace accessions were measured. Pekar slick tests in water and in alkaline conditions were also carried out. A wide variation in color characteristics of the landraces was found, with L values of salted noodle sheets at 2 h ranging from 80.9 to 89.2 and b values of alkaline noodle sheets at 2 h ranging from 19.1 to 27.4, showing potential application in noodle wheat improvement programs. For initial rapid screening of samples it was observed that a single reading of the dough sheet after 2 h was adequate. The dough sheets should be kept at 5 degrees C during storage, to prevent microbiological activity in the dough, which would give erroneous results. The Pekar slick test results were not highly correlated to color measurements on the dough, so this test is not recommended for screening for noodle color potential in landraces.

Catechol Oxidase↗
PubMed1999

[Observation of special stain and ultrastructure of lattice, granular and macular corneal dystrophy].

OBJECTIVE: To investigate the histopathological changes of lattice, granular and macular corneal dystrophy. METHODS: Corneal buttons were obtained from penetrating keratoplasty due to severe corneal dystrophy, and their sections and ultrathin sections with several special stains were observed under a light microscope (LM) and electron microscope (EM). RESULTS: In cases with lattice dystrophy, the stromal fusiform deposits seen under LM were confirmed to be amorphous materiel with filaments by EM and stained positively for Masson trichrome, PAS and Congo red. In cases with granular dystrophy, the granular or bread debris deposits found between laminae under LM were extracellular material observed by EM and they were stained positively for Masson trichrome and alcian blue. In cases with macular dystrophy, the stromal spheroid or macular deposits were seen under LM, and there were numerous vacuoles containing fibrils and granules in the cytoplasm of keratocytes and accumulative amorphous material in the stroma under EM; the deposits were positive for colloidal iron and alcian blue stain. CONCLUSION: In corneal lattice dystrophy, there are amyloid deposits; in granular dystrophy, there are extracellular material deposits; and in macular dystrophy, there is mucopolysaccharide accumulation.

Adult↗
PubMed1999

[A study on pathology and immunoglobulin of orbital tissues from cases with Grave's ophthalmopathy].

OBJECTIVE: To investigate pathological changes and the expression, localization of immunoglobulins (IgA & IgE) in orbital tissues from patients with Grave's ophthalmopathy. METHODS: Orbital tissues were obtained at surgery from 19 patients with Grave's ophthalmopathy and 17 control subjects. Immunohistochemical studies were carried out by using anti-IgA, anti-IgE antibody and a streptavidin-biotin-peroxidase detection system. In addition, hematoxylin-eosin, alcian blue and Masson trichrome stains were employed to demonstrate the pathologic changes in the tissues. RESULTS: 12 of 17 samples from patients exhibited IgA positive staining for the connective tissue associated with the extraocular muscles and orbicularis oculi, however only 2 control muscles reacted minimally. IgE positive material was found in association with the majority of leukocytes and with muscle fibers in 13 patients, but only 4 control subjects displayed mild reactions. Significant expression of IgE was more often demonstrated in patients with eye disease of short course than in those of longer course. CONCLUSION: These results support the notion that the eye muscle fiber and fibroblast are important targets of the orbital autoimmune reaction in Graves' ophthalmopathy in which IgA and IgE both play important roles.

Adolescent↗
PubMed1999

[Study on the removal of arsenic from drinking water by coagulants].

The coagulation method for removing arsenic in drinking water from dispersed water supply was tested. The results showed that the remained arsenic [As(V)] in decanted water could be lower than 0.05 mg/L by directly putting 50 mg/L ferric sulfate into water samples without adjusting pH value (pH 7.82) and settling for 12 hours in room temperature while the original As(V) concentration was 1.0 mg/L. The same removal efficacy could be achieved by putting 30 mg/L ferric sulfate or 40 mg/L aluminum sulfate and filtering after 30-40 min settlement. The removal rates were raised with the increased concentration of coagulants in water. The remained As(V) in filtered water could meet the valid health standard of drinking water (< 0.05 mg/L) when the original concentrations of As(V) were 1.0 mg/L and 0.5 mg/L by using 30 mg/L ferric sulfate and aluminum sulfate respectively. Furthermore, ferric sulfate was more efficient than aluminum sulfate at varied of pH value, water temperature, turbidity and hardness of water, etc. Generally, arsenic in coagulated sludge would not release into water again.

Arsenic↗
PubMed1999

[Effects of pure and crude Lycium barbarum polysaccharides on immunopharmacology].

Effects of pure Lycium barbarum polysaccharides (LBP-X) on immunological activity were compared with crude LBP. The pure LBP-X were divided into different doses, lower doses (5-20 mg/kg.d) of pure LBP-X showed a remarkable effect on immunological enhancement. Especially, LBP-X 10 mg/kg.d had a highly significance difference compared with crude LBP on immune indices in mice. Lycium barbarum polysaccharides have the best pharmacological effect when it was the best dose.

Adjuvants, Immunologic↗
PubMed1999

[The restoration of the "double peak" in OCT images based on inverse Wiener].

It has been found that the high reflectivity film of the reference mirror in OCT may cause a socalled "double peak effect" in the result images. To eliminate this a restoration algorithm is proposed here. The method is based on the mathematical modals of the point-spread function of OCT with consideration of double peak effect. The power spectrum of the noise and signal are estimated by the average smooth periodogram method. An inverse wiener filter algorithm, which avoids the illness condition in common inverse filter, gives the final restored images. The algorithm is also capable of the image degrading recovery due to the aberration and the band-limited spectrum.

Algorithms↗
PubMed1999

[Gas-sensing transmittivity spectrum of two-dimension SnO2: TiO2 compound film in ammonia vapor].

In this paper, we studied the gas-sensing transmittivity spectrum of the two-dimension SnO2: TiO2 compound film in ammonia vapor. It was observed that when the gas concentration was relatively low the transmittivity decreased as the gas concentration decreased, and when the gas concentration was relatively high, the transmittivity increased as the gas concentration increased. We considered the effect of the wet vapor, and explained this phenomenon.

English Abstract↗
PubMed1998

Thin-layer ion-exchange chromatography of proteins.

Thin-layer chromatography (TLC) is one of the simplest and most convenient techniques to separate small molecules. Of a variety of TLC separation modes, only size-exclusion was successfully used to separate proteins. In this paper, adsorption-TLC was used to separate proteins. The net charges were calculated for four model proteins, albumin, transferrin, lactoferrin and lysozyme, under different pH values. The suitable pH values for separation were determined according to the results from such calculations. Then, the adsorption isotherms of the four proteins were measured to deduce the ionic strength for appropriate elution conditions. Optimal conditions, 0.01 M bicine and pH 8.50, and a three-step elution process (1st step 0.01 M NaCl, 2nd 0.025 M NaCl, and 3rd 0.10 M NaCl), were obtained. Finally, the four model proteins were successfully separated under these elution condition.

Adsorption↗
PubMed1998

[Effects of the serum obtained from smoke inhalation injury rats on PMN adhesion and transmigration in vitro].

OBJECTIVE: This study was conducted to determine the changes in PMN adhesive capacity to endothelial cells and transmigration through endothelial cell (CEC) sheet in the presence of the serum from rats with smoke inhalation injury (SII). The effects of adhesion molecules CD11a, CD11b, and ICAM-1 on the PMN adhesion and transmigration were also studied. METHOD: In the present study fluorescent assay, EC sheet cultured on the 5 microns permeable membrane and antibody blocking technique were used to study the PMN adhesion and transmigration in vitro. RESULT: It was found that the serum from SII rats could promote PMN adhesion to CEC sheet. After being cultured with the serum for 12-24 h, PMN adhesion to CEC was more than three times of that in control. The serum also enhanced PMN transmigration through the CEC sheet in vitro, doubling the amount observed in control when the sheet was cultured with the serum for 24 h. Based on the above data it was believed that the SII rat serum could enhance PMN adhesion to and transmigration through lung capillary, and the percentages of PMN adhesion and transmigration were found to be positively correlated (r = 0.975). Furthermore, the antibodies against CD11a, CD11b, and ICAM-1 reduced PMN adhesion 44%, 55% and 51%, respectively, and at the same time decreased PMN transmigration 39%, 65%, and 58%, respectively. CONCLUSION: In the presence of serum obtained from SII rats PMN adhesion to and transmigration through CEC sheet increased, which is the possible underlying mechanism responsible for PMN infiltration in vitro. Moreover, the adhesion molecules such as CDA11a, CD11b, and ICAM-1 play an important role in the process. Infiltrated PMN could release oxygen free radicals and protease to injure lung tissue.

Animals↗
PubMed1998

[The influence of adhesion molecules expression to neutrophil aggregation in liver after severe burns].

OBJECTIVE: To elucidate the mechanism of neutrophil aggregation in the liver and the influence of the expression of adhesion molecules after severe burns during early postburn phase, and to explore simultaneously the mechanism of liver damage after severe burns. METHODS: 1. The morphology of the liver and the activation of MPO were observed. 2. The human hepatocytes and hepatic sinusoidal endothelial cells (HSECs) were isolated and cultured, the flow cytometry and micropipette aspiration system were used to observe dynamically the change in intercellular adhesive force between neutrophils and hepatocytes under the influence of burn patient sera. Also the changes in the expression of P-selectin and ICAM-1 on the hepatocytes and HSECs under the influence of burn patient sera were studied. RESULTS: 1. MPO activation was significantly higher than that in the control group, and reached its peak at 6 hours. Three pieces of liver tissue at 6, 12, 24 hours were examined, and it was found that the Disse's spaces were wide and filled with a great number of PMNs. 2. A constitutive expression of P-selectin and ICAM-1 was found in normal hepatic sinusoidal endothelial cells but not in normal hepatocytes. Under the influence of burn patient sera, the expression of P-selectin increased significantly at 1 hour (P < 0.01) in hepatic sinusoidal endothelial cells but not in hepatocytes, and the expression of ICAM-1 increased significantly at 1 and 2 hour (P < 0.01) in hepatocytes and hepatic sinusoidal endothelial cells. 3. In the presence of burn patient sera, the intercellular adhesive force between neutrophils and hepatocytes reached its peak level at 2 hour after co- cultivation and declined thereafter due to the aggravation of hepatocytic injury. After using ICAM-1 Mab in cultured hepatocytes, the adhesive force reduced significantly. CONCLUSION: Neutrophil aggregation in the liver was confirmed after severe burns during early postburn phase. The expression of P-selectin and ICAM-1 on the hepatocytes and HSECs played key roles to neutrophil aggregation in liver after severe burns during early postburn phase, inducing the participation of neutrophils in hepatocyte injury.

Animals↗