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Biomedical subjects

Q Li

Publications and source records attributed to Q Li.

At least 865 records · Page 48Linked to original sources
PubMed1995

The interaction between "R/S domain" of rat 28S ribosomal RNA and ribosome-inactivating proteins investigated by fusidic acid.

The interaction between "R/S domain" of rat 28S rRNA and ribosome-inactivating proteins (RIPs) has been studied by blocking the action site of RIPs on "R/S domain" of 28S rRNA with fusidic acid or S100. Fusidic acid alone could form stable complexes with rat ribosomes and block the action site of RNA N-glycosidases. incubation of fusidic acid and S100 or GDP with ribosomes could also protect ribosomes from the action of ricin A-chain. However, different effect of fusidic acid, S100 an dGDP was observed when alpha-sarcin was used. Fusidic acid alone could not block the action site of alpha-sarcin. Fusidic acid together with the elongation factors in S100 could block the action site of alpha-sarcin. These results are consistent with the previous report that ricin A-chain and alpha-sarcin recognized different conformation of "R/S domain" in rat 28S rRNA.

Animals↗
PubMed1995

[The infection of hepatitis D virus in adult tupaia].

A new animal model of HDV/HBV infection in adult tupaia (tree shrews) was established to study its pathogenesis and treatment of hepatitis D. In 20 artificially fed adult tupaiae, the positive rate of serum HBsAg was 75% (15/20) after inoculation of human HBV DNA positive serum. After inoculation with positive human serum HBV DNA and positive HDV RNA by way of coinfection and superinfection, serum HBsAg, HDAg and anti-HD were positive one after another in 8 out of 13 tupaiae of the coinfection group and in 6 out of 9 tupaiae of the superinfection group. In some of them HDV RNA was positive in serum or in liver tissue. Transmission of HDV/HBV infection among tupaiae was successful. In group I, animals were inoculated with human HDV/HBV positive serum. In Group II and III, animals were inoculated with tupaia HDV/HBV positive serum of the Group I and II respectively. Serum HBsAg, HDAg and anti-HD became positive one after another in 3 out of 4 tupaiae of the Group II and 4 out 5 tupaiae of the group III. In 2 animals of the Group III, serum HDV RNA was positive and HBsAg and HDAg were detected in the liver tissue. Serum HBsAg was persistantly positive for 16-19 months in the superinfection group. In 2 of them, serum HDAg and anti-HD were alternatively positive and in one of them serum anti-HD was positive persistantly indicating that HDV infection may persist and transform into chronic HDV infection. In the HDV infected tupaiae, there were elevated serum ALT and hepatitis-like pathological changes in the liver tissue which were similar to those in the chimpanzee. We conclude that tupaia may be used as an experimental model of hepatitis D.

Animals↗
PubMed1995

[Effect of pulse current iontophoretic transdermal delivery of insulin on blood glucose in diabetic rats].

Iontophoresis of pulse current with various current intensity, frequency, on/off ratio and duration of treatment was used to facilitate the transdermal delivery of insulin in order to control blood glucose levels in diabetic rats. Male Sprague-Dawley rats were made diabetic by i.p. injection of streptozotocin (65 mg . kg-1 body weight). After two days, diabetic rats were anaesthetized with urethane (as 25% aqueous solution, 1.5 mg . g-1). Both the reservoir electrodes and the receptor electrodes were applied on the abdominal site of the diabetic rats. Iontophoresis was carried out using a prototype transdermal periodic iontophoretic system in order to provide the required direct current with desired pulse modes. The extent of reduction of blood glucose levels was found to be positively correlated with the current intensity, frequency and duration of treatment to some extent. But when the current intensity was over 0.8 mA/cm2 and the frequency was over 3000 Hz, the reduction of blood glucose levels did not continuously increase. Blood glucose levels were found to be better controlled when the on/off ratio of 1 : 1 was used.

Administration, Cutaneous↗
PubMed1995

[The sandwich enzyme-linked immunoabsorbent assay of serum transferrin receptor by using monoclonal and polyclonal antibodies].

The human placenta transferrin receptor was purified in the form of transferrin-transferrin receptor complex (Tf-TfR), and a monospecific polyclonal antibody against TfR was developed by a Tf-coupled Sepharose 4B affinity chromatography to remove the anti-Tf components in the antiserum. A sandwich enzyme-linked immunoabsorbent assay (ELISA) was established for measuring serum transferrin receptor (sTfR) by using monoclonal antibody OKT9 and monospecific polyclonal antibody. This method is simple, specific and sensitive and has a good accuracy. The measurement of sTfR showed that the level of normal children was 4.54 +/- 1.08 mg/L. There were increased levels of sTfR in patients with severe iron deficiency anemia and those with hemolytic anemia (13.92 +/- 4.45 mg/L and 9.94 +/- 3.22 mg/L, respectively). In patients with aplastic anemia, the level was decreased (2.06 +/- 0.82 mg/L). These results indicate that the sTfR measurement has a differential significance for diagnoses of various anemia.

Anemia, Aplastic↗
PubMed1995

[Gamma interferon-like immunoreactivity in adrenal gland and cortical tumor].

Interferon-gamma (IFN-gamma) has many important immunoregulatory functions. It has previously been presumed to be produced by activated lymphoid cells alone. Recently, IFN-gamma like immunoreactivity has been described in certain parts of the nervous system and the skeletal or cardiac muscle system in the rat. We wanted to know whether this molecule could be expressed in the human adrenal gland and cortical tumors. Adrenal tissue samples were taken adjacent to 14 tumor cases, from 17 adrenal cortical adenomas, and 7 cortical carcinoma cases. Deparaffinized 5 microns sections were stained with IFN-gamma monoclonal DB-1 antiserum. The avidin-biotin complex method was used. IFN-gamma appeared in the cytoplasm of tumor cells in 7/7 cases of adrenal cortical carcinoma, 8/17 cases of cortical adenoma and endothelium in 2/7 cases of adrenal cortical carcinoma and 5/17 cases of cortical adenoma. No immunoreactivity was observed in tumor adjacent adrenal gland. Therefore, IFN-gamma is associated with cell differentiation, the higher the immunoreactivity, the lower the differentiation. The capillary endothelium in adrenal cortical tumor tissue may possess receptor for IFN-gamma or can produce IFN-gamma.

Adenocarcinoma↗
PubMed1995

[A radiographic classification for sarcoidosis based on the ILP/UC classification bacillus].

A scheme was devised for semiquantitative description of 79 cases with sarcoidosis using the graphic terminology of International Labour Office and Union International Contre Le Cancer (ILO/CU) for the pneumoconioses. Among 56 patients with stage II, "linear-irregular" categories s t u (50%) and "reticulonodular" categories x y z (28%) predominated while "rounded" categories p q r (22%) were unusual. Among 13 patients with stage III s t u and x y z opacities were found in 38% of patients while p q r only in 23%. The opacities in both stages of sarcoidosis were most severe in the bilateral lower lobes. It was concluded that the traditional method for classification of sarcoidosis was helpful to give the qualitative diagnosis of the disease, but it did not provide objective description of the type and degree of pulmonary involvement. ILO/UC classification was an important supplement of traditional method as ILO/UC could be used to grade the type (linear irregular, rounded and reticulonodular opacities), severity and location of opacities. Therefore, the two methods should be used in combination so as to judge the location, type and severity of intrathoricic sarcoidosis.

Adult↗
PubMed1995

Atomic force microscopy study of fine structures of the entire surface of red blood cells.

Glutaraldehyde-fixed red blood cells were imaged by tapping mode atomic force microscopy (TMAFM) in air at room temperature. The results show that TMAFM can visualize the morphology of the red blood cell at both cellular and nanometer scales. The scan size covers the range from several hundred nanometers to more than one hundred micrometers. TMAFM not only has a higher resolution than the optical microscope, but also can observe biological samples without precoating as required for scanning electron microscopy (SEM). The AFM images of the entire surface of an uncoated red blood cell with nanometer resolution are successfully reconstructed by 28 AFM images of the preselected subareas on the surface of the red blood cell. These images reveal directly the fine structures of the external surface of uncoated red blood cells in air. The surface exhibits a characteristic structure composed of a large number of closely-packed nanometer particles with a size ranging from a few nanometers to tens of nanometers. These "particulate" components are evenly distributed, and no jumping protrusion or depression structures were found. These particles give rise to a very smooth surface of the red blood cell as shown in a large-scan AFM image. In addition, the 28 AFM images obtained by the continuous scanning over 3 hours indicate that TMAFM can image soft biological samples such as red blood cells stably and reproducibly.

Erythrocytes↗
PubMed1995

Primary cerebral lymphoma--a case report and review of the management.

The brain is an uncommon extranodal site for the development of malignant lymphoma. There is an increased risk among immunocompromised patients. While the overall outcome is poor, innovative approaches with combination chemotherapy and radiotherapy can sometimes produce durable remissions. The treatment of a non-immunocompromised patient with cerebral lymphoma incorporating chemotherapy and radiotherapy is described with a review of the literature.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1994

Molecular characterization of the 77-kDa echinoderm microtubule-associated protein. Homology to the beta-transducin family.

The major microtubule-associated protein (MAP) of sea urchins and several other echinoderms is a polypeptide of M(r) 77,000. The echinoderm MAP (EMAP) is abundant in embryonic and differentiated cells, as well as in mitotic and interphase microtubule arrays. To characterize the molecular structure and function of the EMAP, we isolated a full-length cDNA clone, which has one open reading frame that predicts a polypeptide of 686 amino acids with a calculated M(r) of 75,488. On the basis of charge distribution, EMAP can be divided into two distinctive domains: The NH2-terminal basic region (amino acids 1-137, pI = 10.0) and a slightly acidic, COOH-terminal region (amino acids 138-686, pI = 5.8). This charge distribution is typical of many microtubule-binding proteins, but no significant sequence homology has been detected with any known MAPs. The EMAP, however, does show significant sequence similarity with the beta-subunit of the heterotrimeric G-protein, transducin. The homology lies in a series of 10 imperfect, 43-amino acid repeats (WD-40 repeats) that have been found in many proteins of diverse functions, including beta-transducins, Drosophila Enhancer of split, the yeast STE4, CDC4, CDC20, PRP4, and Tup1 gene products, and the dTAFII80 subunit of Drosophila TFIID. The function of these repeats still remains unknown. It is possible that these repeats are involved in protein-protein interactions, perhaps with the tetratricopeptide repeat-containing protein family. Alternatively, the EMAP may be an important link between signal transduction events and a change in microtubule organization during the cell cycle.

Animals↗
PubMed1994

alpha-Amino butyric acid cannot reactivate the silenced gamma gene of the beta locus YAC transgenic mouse.

Butyric acid, a naturally occurring fatty acid, has been shown to increase fetal hemoglobin in BFUe cultures, in primates, and in patients with beta chain hemoglobinopathies. The precise mechanism of gamma gene induction by butyrate is unknown. Butyrate may induce fetal hemoglobin production in vivo by reactivation of silenced gamma globin genes, by inhibiting the silencing of gamma genes, or by both mechanisms. We examined the effects of butyrate on gamma gene expression in transgenic mice carrying three types of constructs: microLCRA gamma mice, which continue to express the gamma gene in the adult stage of development at a level of one-third to one-fifth of the expression in the fetus; microLCRA gamma psi beta delta beta mice, which display correct developmental regulation of gamma and beta human globin genes and have low level gamma globin expression in the adult; and beta locus YAC mice, which display correct developmental regulation of epsilon, gamma, and beta globin genes and have a totally silenced gamma gene in the adult stage. Animals were treated with a continuous infusion of alpha-amino butyric acid (alpha-ABA) for 7 days. In microLCRA gamma mice alpha-ABA produced up to a 43-fold induction of gamma and 9-fold induction of mouse alpha globin genes. In contrast, butyrate did not induce gamma globin expression in the beta locus YAC mice. However, the gamma globin genes of beta locus YAC mice were activated after administration of 5-azacytidine (5-azaC), and the level of gamma globin expression was further increased by administration of alpha-ABA. These results suggest that butyrate cannot reactivate a totally silenced gamma gene and that induction of fetal hemoglobin by this compound may require the presence of preactivated gamma globin genes.

Aminobutyrates↗
PubMed1994

Quantitative analysis of bencynonate in human plasma using a deuterated internal standard by gas chromatography-mass spectrometry with selected-ion monitoring.

A gas chromatographic-mass spectrometric method is described for the quantitative analysis of bencynonate in human plasma. Deuterated bencynonate served as the internal standard and selected-ion monitoring of the fragments of bencynonate and internal standard permitted the quantitation of bencynonate down to 25 pg/ml of plasma. The assay is linear for plasma bencynonate concentrations in the range 25 pg/ml-3 ng/ml. At 0.25 ng/ml the recovery and coefficient of variation are 54.3% and 19.1%, respectively. Application of the method to clinical studies gave data for the pharmacokinetics and relative bioavailability of bencynonate in man.

Animals↗
PubMed1994

Fetal hemoglobin induction by acetate, a product of butyrate catabolism.

Butyrate induces fetal hemoglobin (HbF) synthesis in cultures of erythroid progenitors, in primates, and in man. The mechanism by which this compound stimulates gamma-globin synthesis is unknown. In the course of butyrate catabolism, beta oxidation by mitochondrial enzymes results in the formation of two acetate molecules from each molecule of butyrate. Studies were performed to determine whether acetate itself induces HbF synthesis. In erythroid burst-forming unit (BFU-E) cultures from normal persons, and individuals with sickle cell disease and umbilical-cord blood, dose-dependent increases in gamma-globin protein and gamma mRNA were consistently observed in response to increasing acetate concentrations. In BFU-E cultures from normal adults and patients with sickle cell disease, the ratio of gamma/gamma + beta mRNA increased twofold to fivefold in response to acetate, whereas the percentage of BFU-E progeny staining with an anti-gamma monoclonal antibody (MoAb) increased approximately twofold. Acetate-induced increases in gamma-gene expression were also noted in the progeny of umbilical cord blood BFU-E, although the magnitude of change in response to acetate was less because of a higher baseline of gamma-chain production. The effect of acetate on HbF induction in vivo was evaluated using transgenic mouse and primate models. A transgenic mouse bearing a 2.5-kb mu locus control region (mu LCR) cassette linked to a 3.3-kb A gamma gene displayed a near twofold increase in gamma mRNA during a 10-day infusion of sodium acetate at a dose of 1.5 g/kg/d. Sodium acetate administration in baboons, in doses ranging from 1.5 to 6 g/kg/d by continuous intravenous infusion, also resulted in the stimulation of gamma-globin synthesis, with the percentage of HbF-containing reticulocytes (F reticulocytes) approaching 30%. Surprisingly, a dose-response effect of acetate on HbF induction was not observed in the baboons, and HbF induction was not sustained with prolonged acetate administration. These results suggest that both two-carbon fatty acids (acetate) and four-carbon fatty acids (butyrate) stimulate synthesis of HbF in vivo.

Acetates↗