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Biomedical subjects

Q Li

Publications and source records attributed to Q Li.

At least 847 records · Page 47Linked to original sources
PubMed1995

[Origin of gestation theory in Tibetan medicine].

Generally, it is claimed that gestation theory was originated locally in Tibet, with only a single origin. This author proves that there are two sources, both derived from India. One is a combination of gestation theory from rGyud-bzhi, i.e. Astangahrdayasamhita, and the Great Ratnakuta, and the other, from Somaradza, also derived from the Great Ratnakuta. The dates of writing of modern edition of Somaradza and rGyud-bzhi are still uncertain yet. Based on the linguistic features, this author claims that Somaradza was written before the Tibetan version of Great Ratnakuta (9 century), while rGyud-bzhi, after the translation of Astangahrdayasamhita (10-11 century).

Buddhism↗
PubMed1995

[A 3-year follow-up study of 50 children with acute lymphoblastic leukemia].

Fifty children with acute lymphoblastic leukemia were followed up for an average period of 3 3/12 years. The results showed that 19 (38%) cases were given up after diagnosis. Eight patients only received treatment irregularly for 1 year; among them, 2 discontinued therapy by themselves for 2-3 years and survived free of events, and 6 were lost, Twenty-three received treatment regularly; among them, 3 died of infection, 11 were in continuously complete remission and 9 experienced relapse. The total disease-free survival rate for mean 3 3/12 years is 42% (13/31), exclusive of the 19 given up cases. Statistical analysis indicates a higher relapse rate in those cases with lower initial glucocorticoid receptors (GCR) of peripheral blood lymphocytes.

Child↗
PubMed1995

Bovine fetal-liver stromal cells support erythroid colony formation: enhancement by insulin-like growth factor II.

Stromal cells are one of the components of the hematopoietic microenvironment, which is crucial for the proliferation and differentiation of hematopoietic cells. We have obtained a bovine fetal-liver stromal cell line that supports erythroid colony formation in the presence of erythropoietin. The cells are cytokeratin-negative and vimentin-positive, indicating a mesenchymal origin. Furthermore, they have phagocytic activity and show endothelial-like morphology. Erythropoietin at a concentration of 2 mU/mL was sufficient to increase erythropoiesis in the presence of these stromal cells, and the effect could be further enhanced in the presence of physiological concentrations of insulin-like growth factor II. The supportive role of stromal cells on erythroid colony formation could still be observed, although to a lesser extent, when the erythroid precursors were physically separated from the stromal monolayer by an agar layer or with serum-free media conditioned by stromal cells. Ultrafiltration of the conditioned media indicated that the active factors have a nominal molecular weight smaller than 3 kD, which does not correspond to any of the known cytokines with erythroid-cell stimulating activities. Our data suggest that these fetal-liver stromal cells play an important role in fetal erythropoiesis, a function which was previously thought to be played exclusively by fetal hepatocytes.

Animals↗
PubMed1995

[Preliminary study of a recombinant polyvalent vaccine of Plasmodium falciparum and its immunological activity].

A hybrid gene named HGFC coding three protective antigenic epitopes of Plasmodium falciparum and two exogenous T cell activating epitopes was designed and synthesized. A multicopy hybrid gene named HGF-CAC was also constructed. The two genes were cloned into expression vector pWR450-1 and the hybrid fusion proteins containing forgine antigens and beta-galactosidase were expressed in E. Coli. The molecular weights of the fusion proteins were 65KDa and 77KDa respectively. The expression rate was about 35% of total bacterial proteins. The fusion protein could react specifically with mouse and rabbit antibodies against antigens of Plasmodium falciparum. The rabbit immune serum against the purified fusion protein could specifically recognize the antigens of Plasmodium falciparum and effectively inhibit the in vitro development of the parasites. The inhibitory capacity of the immune sera to parasite invasion was enhanced as the amount of the sera increased and the incubation time of the sera with the parasites was prolonged. After 72h incubation at 20% concentration with the parasites, the serum suppressed the multiplication of parasite to a level of 82% and caused degeneration and death of the parasites. The results indicated that the recombinant hybrid antigen of Plasmodium falciparum has immunological activity and protectivity. It is probably a candidate malaria vaccine.

Amino Acid Sequence↗
PubMed1995

[Expression of chemically synthesized hybrid genes of Plasmodium falciparum and preliminary characterization of expressed products].

The chemically synthesized hybrid genes HGFC and HGFCAC were respectively recombinated with expression vector pWR450-1 and transferred into Escherichia coli. After adding IPTG to the cultural media, the bacteria harboring recombinant plasmid expressed the fusion proteins of exogenous gene products and partial amino acid of beta-galactosidase. The molecular weights of the two fusion proteins were 65 kDa and 77 kDa, respectively. The expressed fusion proteins could be recognized by antibodies of rabbit anti-RESA peptide EENVEHDA by Western blotting, indicating that the fusion proteins harbored the antigenic epitopes of Plasmodium falciparum.

Animals↗
PubMed1995

[Clinical observation of posterior chamber lens implantation in children].

Extracapsular cataract extraction with posterior chamber intraocular lens (IOL) implantation was performed on 22 eyes of 16 patients with congenital or traumatic cataract and with ages ranging from 4 to 7 years old. The periods of follow-up were 1-2 years with a mean, 18 months. The postoperative reaction was mild, no untoward complications occurred in the remote follow-up and the postoperative visual acuities recovered well. The youngest age of IOL implantation, the choice of diopter of refraction and the management of intra- and post-operative complications were discussed.

Age Factors↗
PubMed1995

Swallowing and esophageal function in Parkinson's disease.

Dysphagia and drooling of saliva are frequent symptoms in Parkinson's disease (PD), occurring in one-half and three-quarters of all patients, respectively. Aspiration related to swallowing is a major cause of morbidity and mortality in PD. Defects in oral, pharyngeal, and esophageal phases of swallowing have been documented in patients with PD, and these defects precede symptoms. This paper reviews the current knowledge concerning swallowing abnormalities in PD. The pathogenesis of dysphagia and drooling of saliva is multifactorial, involving cognitive and psychological changes in addition to abnormalities of the extrapyramidal and autonomic nervous systems. Videofluoroscopic imaging of the upper esophageal sphincter and pharynx during mastication and swallowing has been the basis of our understanding of the mechanical malfunction present in patients with PD. Manometric abnormalities of the esophageal body and lower esophageal sphincter have also been documented. The use of combined manofluoroscopy to examine the upper esophageal sphincter and pharynx in PD offers great promise both in understanding the defects and directing therapy. Voluntary airway protection techniques may reduce aspiration, but they need to be tested in a clinical study. Such maneuvers may reduce the morbidity seen in PD.

Deglutition↗
PubMed1995

[Effect of electric fields on the proteins in nerve regeneration conditioned fluid].

80 SD rats were randomly divided into four groups of 20 each: local electrostimulation of nerve stump (Group LS); electro-stimulation of myeloneure (Group N); electrostimulation of the denervated muscle (Group M); and controls (Group C). The left lateral sciatic nerve of rats was excised 5mm in length, and the severed nerve was bridged with the silicon tube. The gap between the stumps was about 10mm. At 3rd, 7th, 14th, 21st and 28th day after nerve transection and tube implantation, the fluid in the silicon tube was aspirated. The sample of fluid was spun and 4 microliters aliquots were taken from the supernatant. Then the electrophoresis of the aliquots was made by phase-system and assayed to the amount and variety by Gel Scan system. The results showed that the group LS had a high increase in the protein amount than others in the range of 6.16-10.23 x 10(3)D molecular weight. We believe that it is one of the mechanisms of the electric fields promoting nerve regeneration.

Animals↗
PubMed1995

Detection of human papillomavirus types 16, 18 DNA related sequences in bronchogenic carcinoma by polymerase chain reaction.

In studying the relationship between human papillomavirus (HPV) and bronchogenic carcinoma, "high-risk" HPV 16, 18 DNA sequences were detected in samples from 50 lung cancer patients, 18 patients with benign pulmonary diseases and 4 fetal lung tissues by polymerase chain reaction (PCR) and dot-blot hybridization with biotin-labelled probes. The results showed that HPV 16, 18 DNA related sequences were found in 32% of lung cancer specimens, with 10 cases of HPV 16, 5 cases of HPV 18 and 1 case of both types. 48.15% (13/27) of squamous cell carcinomas were shown to be positive for HPV 16, 18 DNA. In addition, two adenocarcinomas and one small cell carcinoma were positive for HPV 16 DNA. No specimens from benign diseases tissues and fetal lung tissues showed positive results. These results suggest that primary bronchogenic carcinoma is related to HPV infection.

Adenocarcinoma↗
PubMed1995

[Dynamic observation of alpha-granule membrane protein 140 during the treatment of thrombolytic and anticoagulant therapy in patients with acute myocardial infarction].

The degree of platelet activation and damage in 15 cases with acute myocardial infarction (AMI) receiving thrombolytic therapy and 15 cases with AMI receiving anticoagulant therapy were studied in vivo and in vitro by using specific monoclonal antibodies (SZ-51 & S12) against alpha-granule membrane protein 140 (GMP-140). Clinical indexes and myocardial enzyme changes in the two groups of patients were also observed. The results showed that the number of GMP-140 molecules on platelet surface and the concentration of GMP-140 in plasma were increased before treatment. The number of GMP-140 molecules on platelet surface began to decrease on the 1st day and returned to baseline on the 7th day after treatment. The concentration of GMP-140 in plasma reached a peak on the 1st day, began to fall on the 2nd day and returned to baseline on the 3rd day after treatment. There were no significant differences in the dynamic changes of number of GMP-140 molecules on platelet surface and the concentration of GMP-140 in plasma between groups of thrombolytic therapy and anticoagulant therapy. In vitro experiment showed that the thrombolytic medicine urokinase neither activated platelets nor inhibited platelet activation induced by thrombin. Significantly greater reperfusion rate and earlier appearance of CK and CK-MB peaks were found in the thrombolytic than in the anticoagulant group. LVEF determined by echocardiography, rate of return of ST segments to baseline and alleviation rate of chest pain were significantly greater and complications of AMI (ventricular fibrillation, left ventricular failure and angina) were less in the group receiving thrombolytic therapy.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1995

[Successful replantation in ten-digit complete amputation: a case report].

We report a case of successful replantation of ten-digit complete amputation. The conditions of the wounds over the ends of the ten amputated digits varied from severe to light and easy for replantation. The amputation included severe avulsion injuries over four fingers. Appropriate measures were taken during surgery and the replanted digits all survived. The replantation operation was done by a team of surgeons. Although the surgeons were physically challenged by the long and difficult procedures, they worked hard for the benefit of the patient. Physiotherapy and exercises six months after operation showed that the functions of both hands were largely recovered.

Adult↗
PubMed1995

Low density lipoprotein-cholesteryl ester-derived linoleic acid is mainly incorporated into the phospholipid component of the macrophages.

The cellular metabolism of the cholesterol in the low density lipoprotein cholesteryl ester (LDL-CE) moiety is well characterized, whereas the cellular fate of the fatty acid (mainly linoleic acid) in the LDL-CE has not been studied in detail. The distribution of the LDL-CE-derived linoleic acid among cellular lipids was studied in J-774 A.1 macrophages, using LDL that was radiolabeled in the linoleic acid of its CE moiety. Macrophages were incubated with radiolabeled LDL for 4 h at 4 degrees C, washed and further incubated for up to 24 h at 37 degrees C in a fresh medium (without LDL). The distribution of the linoleic acid among cellular lipids was then analyzed. After 20 min of incubation, most of the linoleic acid was found in the CE fraction as a constituent of the internalized LDL, and the CE-associated linoleate was progressively decreased. In parallel, the linoleic acid was found to be esterified into the macrophage phospholipids (mostly in the macrophage phosphatidyl choline fraction), accounting for up to 62% of the total cellular labeled linoleic acid after 24 h of incubation. We conclude that the fatty acid derived from the hydrolysis of the LDL-CE moiety in macrophages is mainly incorporated into the cellular phospholipids where it can serve for various cellular metabolic processes.

Animals↗
PubMed1995

[Facilitated transdermal delivery of insulin by pulse current iontophoresis].

Facilitated transdermal delivery of insulin by pulse current iontophoresis was investigated. It was found that pulse current iontophoresis can increase the transdermal permeation rate, and there was a positive correlation between reservoir insulin concentration and skin permeation rate of insulin. Moreover, when the reservoir solution pH (3.6) was below the isoelectric point of insulin (pI 5.2), the transdermal permeation rate of insulin was the highest, 324.3 +/- 33.4 microU/(cm2.h). When the pH of the reservoir solution was brought up to 7.4, the transdermal permeation rate of insulin declined markedly to 143.7 +/- 27.3 microU/(cm2.h). When the reservoir solution pH was close to the isoelectric point of insulin, the transdermal permeation rate of insulin was the lowest, 78.4 +/- 21.9 microU/(cm2.h).

Animals↗
PubMed1995

Fluorescence spectroscopic study of the interaction of adenine and nucleotide with trichosanthin.

Trichosanthin (TCS) is an N-glycosidase that can attack the 28s rRNA of the ribosome at a highly conserved adenine residue. The interactions of adenine and its derivative nucleotides with TCS are reported. The fluorescence of Trp 192 of TCS is sensitive to the proximity of adenine, and produces a marked red shift indicative of trytophan in a more hydrophilic environment. By contrast AMP and ATP quench the maximal emission at 328nm. The binding of the adenine and ATP with TCS result in lower tryptophan accessibility to the quencher acrylamide, but higher tryptophan accessibility to the quencher iodide, while AMP caused higher tryptophan accessibility to acrylamide, and lower tryptophan accessibility to iodide. Also, the binding of nucleotides induces tryptophan heterogeneity in the protein. These findings lead us to propose that binding of nucleotides and adenine base cause different microenvironmental changes of the tryptophan residue, and Trp 192 may be involved in the active site of TCS.

Adenine↗
PubMed1995

[The inhibitory effects of immune sera against two recombinant hybrid antigens on the growth of Plasmodium falciparum in vitro].

The inhibitory effects of rabbit immune sera against two recombinant hybrid antigens (C and CAC) of Plasmodium falciparum to the multiplication and development of in vitro cultured P. falciparum parasites were tested. Although both of anti-C and anti-CAC immune sera had inhibitory effect on parasites, the anti-CAC serum showed much greater effect than that of anti-C serum (P < 0.05). The inhibitory capacities in the sera to parasites were enhanced with the increase in the serum concentration as well as with the prolongation of the incubation time of the sera with the parasites. It was shown that after 72 h incubation of the anti-CAC serum with the parasites at the concentration of 1%, 10% and 20%, the inhibition rates were 15%, 54% and 82%, respectively. The immune sera caused the dispersion of parasite cytoplasm, atrophy of parasites, agglutination of free merozoites and degeneration of schizonts, suggesting that the hybrid antigen could produce multi-functional protective antibodies against antigens of Plasmodium falciparum.

Animals↗
PubMed1995

Lipoprotein receptor interactions are not required for monocyte oxidation of LDL.

Upon activation, human peripheral blood monocytes and U937 cells oxidized low density lipoprotein (LDL), converting it to a cytotoxin. The oxidized LDL loses its ability to interact specifically with the native LDL (apoB/E) receptor and becomes a ligand for the scavenger receptors and two other receptors, Fc gamma RII (CD32) and CD36. We performed a series of studies to evaluate the potential contribution of each of these receptors to the process of monocyte-mediated LDL oxidation. To assess the participation of the apoB/E receptor, we tested the ability of activated human monocytes to oxidize LDL after up- and down-regulation of apoB/E receptors. Neither up-regulation nor down-regulation of the apoB/E receptor significantly modified the level of LDL lipid oxidation. Acetylated LDL, a ligand for scavenger receptors, was also oxidized by the activated monocytes. Methylated LDL, a chemically modified LDL that is not recognized by the apoB/E or scavenger receptors, was oxidized as well. Thus, LDL does not need to interact with either the apoB/E receptor or scavenger receptors in order to undergo lipid oxidation. Additionally, monoclonal antibodies to CD36 and CD32 were used to block these two receptors that recognize oxidized LDL. Although both antibodies interfered with oxidized LDL binding to these receptors, neither treatment interfered with LDL lipid oxidation mediated by activated human monocytes. Our results suggest that interaction with these receptors is not a requirement for LDL lipid oxidation by activated human monocytes.

Acetylation↗