Biomedical subjects

P Zou

Publications and source records attributed to P Zou.

At least 37 records · Page 2Linked to original sources

PubMed1999

Immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding section of the bone marrow from patients with myelodysplastic syndrome and its clinical implication.

An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative and quantitative immunohistochemical analysis are as follows: (1) Labile antigens of T lymphocytes were well preserved, thus allowing analysis of distribution of T lymphocyte subsets in situ: (2) the average number of T3, T4 and T8 lymphocyte of the diffuse infiltrate was about 2%, 0.4%, 0.5%, respectively, of all nucleated cells in bone marrow, and T4/T8 of T cells were below 1.0 in patients with MDS; (3) there were cases of RAS showing T lymphocyte aggregation in bone marrow, but no patient exhibited progressive refractory anemia with excess of blasts (RAEB) and RAEB in transformation (RAEBT). These findings indicated that the immunological abnormalities are of importance in the evaluation of pathogenesis and prognosis of MDS.

Aged↗

PubMed1999

Comparison of the complete DNA sequences of human herpesvirus 6 variants A and B.

Human herpesvirus 6 (HHV-6), which belongs to the betaherpesvirus subfamily and infects mainly T cells in vitro, causes acute and latent infections. Two variants of HHV-6 have been distinguished on the basis of differences in several properties. We have determined the complete DNA sequence of HHV-6 variant B (HHV-6B) strain HST, the causative agent of exanthem subitum, and compared the sequence with that of variant A strain U1102. A total of 115 potential open reading frames (ORFs) were identified within the 161,573-bp contiguous sequence of the entire HHV-6 genome, including some genes with remarkable differences in amino acid identity. All genes with <70% identity between the two variants were found to contain deleted regions when ORFs that could not be expressed were excluded from the comparison. Except in the case of U47, these differences were found in immediate-early/regulatory genes, DR2, DR7, U86/90, U89/90, and U95, which may represent characteristic differences of variants A and B. Also, we have successfully typed 14 different strains belonging to variant A or B by PCR using variant-specific primers; the results suggest that the remarkable differences observed were conserved evolutionarily as variant-specific divergence.

DNA, Viral↗

PubMed1999

Human herpesvirus 6 open reading frame U83 encodes a functional chemokine.

Some viruses including herpesviruses have undergone evolution to benefit viral infection and propagation by pirating and modifying host genes such as chemokine genes. Human herpesvirus 6 (HHV-6), acutely or persistently infects mononuclear cells in vitro. DNA sequence analysis of HHV-6 has revealed that the putative protein encoded by an open reading frame (ORF) of the U83 gene in HHV-6 variant B resembled a human chemokine. We have cloned the U83 gene and analyzed the biological function of this gene. The U83 gene contained an ORF encoding a 113-amino-acid peptide, starting at the first methionine and containing a possible signal peptide and the typical cysteine residues characteristic of the chemokines. Reverse transcription-PCR analysis of mRNA and immunofluorescent-antibody testing of infected cells both indicated that the encoded protein was a late protein. The ORF U83 gene fused to the Fc gene was expressed as a fusion protein in COS-7 cells by transfection, and the fusion protein was purified from the supernatant of transfected cells to test its biological function. The purified protein was capable of inducing transient calcium mobilization in THP-1 cells and of chemotactically activating THP-1 cells. These findings suggested that the U83 protein might play an important role in HHV-6 propagation in vivo by activating and trafficking mononuclear cells to sites of viral replication, thus aiding the development of superbly efficient virus production mechanisms.

Amino Acid Sequence↗

PubMed1999

[Distribution of six STR loci in Bai ethnic group in Yunnan province of China].

OBJECTIVE: This study was conducted to make known the distribution of six STR loci in Bai ethnic group in Yunnan province. METHODS: DAN extraction from blood samples (126 in number ) and multiplex amplification of CSF1PO,TPOX, TH01,F13A01, FESFPS and vWA were carried out. Using denaturing polyacrylamide gel electrophoresis and silver stain, the authors investigated the distribution of allele frequencies of CSF1PO, TPOX,TH01,F13A01,FESFPS and vWA loci in Bai ethnic group living in Yunnan province. RESULTS: 7 alleles and 19 genotypes of CSF1PO locus, 5 alleles and 11 genotypes of TPOX locus, 6 alleles and 16 genotypes of TH01 locus 6 alleles and 16 genotypes of F13A01 locus, 7 alleles and 15 genotypes of FESFPS locus,and 7 alleles and 21 genotypes of vWA locus were observed. CONCLUSION: The allele distribution of the loci was in good agreement with the Hardy-Weinberg equilibrium.

China↗

PubMed1999

Dauricine inhibits redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin induced by thrombin.

AIM: To study the possibility of dauricine (Dau) inhibiting redistribution of platelet membrane glycoprotein IV (GPIV) and release of intracellular alpha-granule thrombospondin (TSP) on platelet activation. METHODS: Using the flow cytometric assay of washed platelet to record expression of GPIV and release of TSP induced by thrombin. RESULTS: Dau did not affect GPIV and TSP on resting platelet membrane but inhibited redistribution of GPIV to the platelet surface and TSP release on activated platelet. There was a marked positive correlation between changes of GPIV and TSP (r = 0.511, P < 0.01). The inhibitory effect of Dau appeared not to be Ca2+ concentration-dependent. CONCLUSION: Dau inhibited redistribution of GPIV and release of intracellular alpha-granule thrombospondin induced by thrombin.

Adult↗

PubMed1998

[Distribution of three STR loci in Dai ethnic group in China].

OBJECTIVE: This study was conducted to investigate the distribution of three STR loci in Dai ethnic group. METHODS: Multiplex amplifications of CSF1PO, TPOX and TH01 were carried out. Using denaturing polyacrylamide gel electrophoresis and silver staining the authors investigated the distribution of allele frequencies of CSF1PO, TPOX and TH01 loci in Dai ethnic group living in Yunnan province. RESULTS: 7 alleles and 15 genotype of CSF1PO locus, 7 alleles and 18 genotypes of TPOX locus, 6 alleles and 17 genotypes of TH01 locus were observed. CONCLUSION: The allele distribution of the loci is in good agreement with the Hardy-Weinberg equilibrium.

China↗

PubMed1998

Gene transfer into hematopoietic cells of mouse and its in vivo expression after transplantation.

We have shown previously that high-efficient gene transfer can be attained in primary hematopoietic cells using liposome-mediated gene transfer strategy. In order to examine the stability of gene expression mediated by this gene transduction protocol, we observed the expression of marker gene in vivo by using bone marrow transplantation (BMT) to engraft lethally irradiated mouse with the genetically modified hematopoietic cells. The results showed that the mouse transplanted with appropriated number of transduced cells remained alive and healthy. The PCR analysis and G418 selection of the spleen colonies and bone marrow cells isolated from lethally irradiated animals 15 days and 30 days after injection of genetically modified bone marrow cells showed that the progeny cells of the transduced hematopoietic stem cells still contained and expressed the transduced genes, suggesting that the hematopoietic system is at least partially re-constructed by the stem cells with marker gene and that the stable expression of foreign genes in vivo can be attained by using this easy and harmless transduction protocol. These findings provide experimental basis for clinician to further investigate the biology of marrow reconstruction and the mechanism of leukemia relapse after BMT.

Animals↗

PubMed1998

Bone morphogenetic protein and ceramic-induced osteogenesis.

To investigate the relationship between bone morphogenetic proteins (BMP) and calcium phosphate ceramic-induced osteogenesis in soft tissues, in vitro and in vivo experiments were performed. In an in vitro study, the ability of different calcium phosphate ceramics to absorb bovine BMP (bBMP) from a bBMP solution was tested. In vivo studies included immunohistochemical BMP staining before bone formation in the ceramics was detected, and the enhancement of bone formation in calcium phosphate ceramics by bBMP. The results were: (1) calcium phosphate ceramics have a strong ability to absorb bBMP; (2) a high BMP concentration reaches inside the ceramic implants before bone formation in soft tissues of domestic pig occurs; (3) by 56% at 50 d and by 23% at 100 d, bBMP enhances bone formation in calcium phosphate ceramics implanted in soft tissues of dogs. The results indicate the BMP plays an important role in calcium phosphate ceramic-induced osteogenesis and that adsorption of native BMP from the body fluids to ceramic implants may be a key step in osteoinduction by calcium phosphate ceramics.

Journal Article↗

PubMed1998

[Experimental study on the effect of Chinese traditional medicine "bone growth fluid" in the change of trace elements in bone lengthening area].

In order to study the effect of Chinese traditional medicine, "Bone Growth Fluid", on bone formation in bone lengthening, the limb lengthening model was made on goat to observe bone formation in the distracted area, and the content of the trace elements was determined. The bone-lengthening operation was carried out on the upper metaphysis of left tibia. The animals were divided into two groups following operation. From 2nd day afteroperation, "Bone Growth Fluid", 10 ml per kilogram body weight, was given daily to goats in the experimental group, and same amount of normal saline was given to goats in another group as control. The results showed-that new bone formation and bone remodeling in the experimental group appeared earlier than that in the control group, and the content of the trace elements was also improved. So Chinese Traditional medicine, "Bone Growth Fluid", could accelerate the accumulation of the trace elements in callus on the distracted sites and it might play some role in the promotion of osteogenesis and bone remodeling in bone lengthening.

Animals↗

PubMed1998

Activity of platelet in patients with high level of LDL and the effect of LDL on platelet glycoproteins.

OBJECTIVE: To investigate the activity of platelet in patients with high level of low density lipoproteins (LDL) and the effect of LDL on platelet glycoproteins (GP). METHODS: Platelet glycoproteins, whose platelet were activated or unactivated, were measured by flow cytometry. RESULTS: The amount of the platelet membrane GP II b/III a in patients was not significantly different from that of the control when platelet was unactivated (P > 0.05); when platelet was activated by adenosine diphosphate (ADP), the amount of patients' platelet GP II b/III a was increased markedly in comparison with that in the control (P < 0.01). Having been preincubated with LDL, the platelets were activated by ADP and the amounts of GP II b/III a of patients group and the control were all increased obviously as compared with those in the platelets which were not incubated with LDL (P < 0.01; P < 0.01). There was no significant difference of the amount of granule membrane protein-140 (GMP-140) between patients and the control when platelet was activated by thrombin (P > 0.05). Having been preincubated with LDL, platelet was activated by thrombin, the amounts of GMP-140 on patients' and control platelet were all increased markedly (P < 0.01, P < 0.01). CONCLUSION: The activity of platelet in patients with high level of LDL is increased significantly. LDL can increase the expression of platelet glycoproteins.

Adult↗

PubMed1998

[Distribution of F13A01, FESFPS and vWA loci in Jingpo ethnic group in Yunnan provice].

F13A01 FESFPS and vWA were extracted and amplified using denaturing polyacrylamide gel electrophoresis and silver stain. The distribution of allete frequencies of F13A01, FESFPS and vWA loci in Jingpo ethnic group living in southwestern Yunnan province were investigated. It was observed that there were 8 alleles and 13 genotypes in F13A01 locus; 7 alleles and 18 genotypes in FESFPPS locus; 7 alleles and 21 genotypes in vWA lous.

Alleles↗

PubMed1997

Redistribution of platelet membrane glycoprotein IV and release of intracellular alpha-granule thrombospondin in patients with chronic myelogenous leukemia.

The redistribution of platelet membrane glycoprotein IV (GPIV) and the release of intracellular alpha-granule thrombospondin (TSP) were examined and the inhibition of beta-thromboglobulin (beta-TG) and platelet factor 4 (PF4) in patients with chronic myelogenous leukemia (CML) was observed and quantitation of beta-TG and PF4 in sera was conducted. GPIV in inactive platelet from CML was 36080 +/- 17010 molecules/platelet as compared with 13190 +/- 4810 from the controls (P < 0.01). No abnormality was found in the distribution of platelet membrane GPIb and GPIIb/IIIa (P > 0.05). The GPIV redistribution on active platelet membrane induced thrombin (IU/ml) from CML and healthy donors was 44320 +/- 32310 and 22800 +/- 12700 molecules/platelet respectively (P < 0.01). The difference in the release of intracellular alpha-granule TSP between CML and the control group was not found (P > 0.05). There was no direct correlation between GPIV expression and TSP binding after platelet activation. The high levels of beta-TG and PF4 in sera inhibited release of intracellular alpha-granule TSP in vitro. These results indicate that the abnormality of platelet membrane GPIV is a common marker in CML, therefore the specific increase of platelet GPIV in patients with CML may be a useful tool for the diagnosis and monitoring of the platelet dysfunction. The release of internal TSP pools is hindered by either beta-TG or PF4 in sera.

CD36 Antigens↗

PubMed1997

Osteogenic responses to extraskeletally implanted synthetic porous calcium phosphate ceramics: an early stage histomorphological study in dogs.

In this experiment, synthetic porous calcium phosphate ceramics (hydroxyapatite-tricalcium phosphate) were prepared and implanted in dorsal muscles of dogs. The purpose was to study the biological processes prior to and during the morphogenesis of bone in extraskeletally implanted porous calcium phosphate ceramics. Specimens were harvested after implantation for 7, 15, 30, 45, 60, 90 and 120 days. Decalcified and undecalcified sections were prepared for alkaline phosphatase (ALP) histochemical localization and comparative histological analysis. The results show that bone morphogenesis in the pore regions of the extraskeletally implanted ceramics follows a complex process involving clot formation, vascular invasion, granulation-like tissue formation, polymorphic cell aggregation, osteoblast differentiation and bone formation. The characteristic feature preceding bone formation was polymorphic cell aggregation on the pore inner surface and near the invading capillaries or small venules. These cells were of various sizes and shapes, and some of them were positive for ALP activity. ALP-positive cell aggregates were more numerous where capillaries or venules were close to the pore inner surface. Osteoblast differentiation occurred within the cell clusters aggregated on the pore inner surface and bone matrix was secreted in direct contact with the ceramics. During bone formation, capillaries or small venules were always found close to the developing fronts of the osseous nidi. It is suggested that those cells which first appeared near the invading vasculature, the cells which aggregated on the pore inner surface and those cells which finally differentiated into osteoblasts may be interrelated in some way.

Journal Article↗

PubMed1997

[GM-CSF regulates the Vp16 induced leukemic cell apoptosis].

OBJECTIVE: To explore the regulatory effects of granulocyte-macrophage colony stimulating factor (GM-CSF) on the apoptosis of leukemic cells induced by Vp16. METHODS: High expression retrovirus vector, N2A/CMV/GM-CSF, was constructed and transferred into human leukemic cell line HL-60, and Vp16 (final concentration 10microg/ml) was administered to transferred or nontransferred HL-60 cells. RESULTS: After Vp16 treatment, characteristic changes for apoptosis emerged in HL-60 cells. However, these findings did not emerge in GM-CSF gene transferred HL-60 cells. CONCLUSION: GM-CSF can suppress the leukemic cell apoptosis induced by Vp16.

Apoptosis↗

PubMed1997

[Study on the relationship between the expression of adhesion molecules and the invasiveness of acute myeloid leukemia cells].

OBJECTIVE: To study the relationship between the expression of adhesion molecules CD49d (VLA-4) and CD11a (LFA-1) and the invasiveness of acute myeloid leukemia (AML) cells. METHODS: Peripheral blood and/or bone marrow samples from 50 AML patients were investigated by APAAP and Western blotting method. RESULTS: Extramedullary invasion developed in 32 of 50 patients (64%). The expression of CD49d and CD11a in the invasive group was much higher than that in the non-invasive group (P<0.005), while the difference between the leukemic cells from bone marrow and peripheral blood for CD49d/CD11a expression was not significant. CONCLUSION: AML cells might adhere to and get through vascular endothelium by CD49d/VCAM-1 and CD11a/ICAM-1 adhesion mechanism, and the expressions of CD49d and CD11a were not critically responsible for the release of leukemic cells from bone marrow.

Adolescent↗

PubMed1997

[Liposome-mediated gene transfer into bone marrow cells and its in vitro expansion].

OBJECTIVE: To explore the efficiency of liposome-mediated gene transfer into bone marrow cells and the stability of its expression. METHODS: Two marker genes (Neo and Lac Z) mediated by liposome were co-transferred into bone marrow cells. Following G418 screening,the positive cells were enriched and expanded in vitro. RESULTS: The liposome-mediated gene transfer rate in bone marrow cells was 13.33 +/- 2.68%, and after G418 screening, it was 46.06 +/- 3.47%. The blue colouring rates with X-gal staining of these cells were not significantly different before and after expansion. CONCLUSION: Liposome-mediated gene transfer in bone marrow cells is highly efficient and the expression of the transferred gene is stable.

Bone Marrow Cells↗

PubMed1996

Osteogenesis in extraskeletally implanted porous calcium phosphate ceramics: variability among different kinds of animals.

Synthetic porous calcium phosphate ceramics (HA/TCP) were implanted intramuscularly and subcutaneously in dogs, pigs, goats, rabbits and rats, designed to make a comparative study of the host tissue responses to porous HA/TCP ceramics in different kinds of animals. Specimens were harvested at 15, 30, 45, 60, 90 and 120 days after implantation. Decalcified and undecalcified sections were made and examined by light microscopy. Obvious bone formation could be detected in some specimens harvested from dogs and pigs after 45 days intramuscular implantation or after 60 days subcutaneous implantation. At days 90 and 120, an extensive amount of bone formed in all specimens implanted in dogs and pigs. However, no histologically detectable bone formation was observed in any specimen implanted intramuscularly and subcutaneously in goats, rabbits and rats until 120 days. It is demonstrated from this finding that the synthetic porous calcium phosphate ceramics are capable of inducing osteogenesis when implanted in non-bony sites, but this ability varies between different kinds of animals. Earlier periods of observation in specimens harvested from dogs showed that bone differentiation in the pore regions of the ceramics follows a complex process involving invasion of the fibrovascular connective tissues at day 15, appearance of polymorphic mesenchymal cells near the invading vasculature and at the interface with the ceramics at day 30, differentiation of osteoblasts and formation of bone matrix in direct contact with the surface of the ceramics at day 45, and finally remodelling of the fibrous connective tissue into an extensive amount of bone at days 60, 90 and 120.

Animals↗

PubMed1995

[Analysis of human DNA fingerprints by using fluorescein labelled probe JH12.6].

A method of analysis of human DNA fingerprints has been established by using fluorescein (Fl-11-dUTP) labelled probe JH12.6. The clear and high distinctive patterns of human DNA fingerprints were obtained with this method. The DNA fingerprints of 78 unrelated individuals living in Yunnan province of China were detected, and their probability of chance association between random individuals was calculated as 7.4 x 10(-11), and their mean allelic frequency as 0.09. Comparing of DNA fingerprints with labelling probe JH12.6 by using between radioactive 32P and fluorescein showed that the method by using fluorescein labelled probe JH12.6 was simple, fast, safe and economical. It can totally replace the method by using radioactive 32P labelled probe JH12.6, and it can be broadly applied in forensic medicine and other fields.

DNA Fingerprinting↗