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Biomedical subjects

P Sureau

Publications and source records attributed to P Sureau.

At least 55 records · Page 3Linked to original sources
PubMed1987

The association of the rabies glycoprotein with liposome (immunosome) induces an in vitro specific release of interleukin 2.

BALB/c mice were primed by receiving a unique intraperitoneal injection of rabies virus antigens presented as complete inactivated virus (P.V. strain) or as purified glycoproteins either in the aggregated form or in physical combination with liposomes (i.e., in the form of "immunosomes"). The splenocytes of these mice were restimulated, 6-15 days after priming, in culture with rabies virus antigens, and antigen-specific IL-2 production was measured. It was found that rabies antigens presented as immunosomes were as active as the inactivated virus, whereas equivalent amounts of purified glycoproteins were inactive. The optimal amounts of rabies immunosomes used for priming was found to be 0.5 to 0.05 micrograms per mouse.

Animals↗
PubMed1987

A collaborative study of an experimental kit for rapid rabies enzyme immunodiagnosis (RREID).

Six laboratories took part in a study to assess an experimental kit for the diagnosis of rabies using the rapid rabies enzyme immunodiagnosis (RREID) technique. The test is based on the immunocapture of rabies antigens present in homogenized brain specimens, followed by enzyme immunoassay. A total of 1253 specimens from various geographical locations and 27 animal species were tested with the RREID technique, and also with the fluorescent antibody test (FAT), which was used as a reference method. For 1220 specimens the results in RREID and FAT were the same (651 positive and 569 negative-concordance: 97.4%). However, the RREID technique appeared to be less sensitive, since 22 (3%) of the 673 specimens that were positive with FAT were negative with RREID. The RREID test is therefore specific and convenient and is a useful tool for epidemiological studies and for laboratories not equipped with an ultraviolet microscope.

Animals↗
PubMed1986

A rapid rabies enzyme immuno-diagnosis (RREID): a useful and simple technique for the routine diagnosis of rabies.

A Rapid Rabies Enzyme Immuno-Diagnosis (RREID) technique has been developed. This technique for the diagnosis of rabies was performed in microplates which had been previously sensitized with IgG to purified antinucleocapsids. Suspensions of homogenized material were incubated in the plate and the specific binding of rabies antigen was revealed by the use of the same IgG conjugated with peroxidase. With the RREID technique it was possible to detect rabies antigens in brain specimens with the same specificity and sensitivity as that of the direct immunofluorescence test or the neuroblastoma cell inoculation technique regardless of the species of animal from which the specimen was derived. Moreover, RREID was performed with fox salivary gland specimens with the same results as were obtained with brain specimens. RREID does not require an UV light microscope and a photometer is not essential. It is a useful and simple technique for the routine laboratory diagnosis of rabies.

Animals↗
PubMed1986

The influence of the type of immunosorbent on rabies antibody EIA; advantages of purified glycoprotein over whole virus.

Two types of in vitro assay (enzyme immunoassay and sero-neutralization test) for the titration of rabies antibodies were used to assay sera from mice and humans immunized with cell culture vaccines or neural tissue vaccines. Enzyme immunoassays (EIA) were performed in plates sensitized with whole virus, purified glycoprotein or purified nucleocapsid. Neutralizing antibody titres were determined by the rapid fluorescent focus inhibition test (REFIT) and by an in vitro seroneutralization test including a rapid enzyme immunotitration of intracellular antigens (REITICA). The results obtained with sera of immunized mice and humans showed that (1) cell culture vaccines mainly induced the synthesis of antiglycoprotein neutralizing antibodies; and (2) neural tissue vaccines induced a high synthesis of antinucleocapsid non-neutralizing antibodies and a more or less important synthesis of antiglycoprotein antibodies depending on the origin of the tissue used for their preparation. Consequently, it was emphasized that when using EIA, the antibody titration must be run in glycoprotein-coated plates rather than in whole virus-coated plates to appreciate correctly the immunizing potency of a rabies vaccine, especially neural tissue vaccine.

Animals↗
PubMed1986

An evaluation of second generation tissue culture rabies vaccines for use in man: a four-vaccine comparative immunogenicity study using a pre-exposure vaccination schedule and an abbreviated 2-1-1 postexposure schedule.

In a double-blind comparative trial the immunogenicity of three new tissue culture rabies vaccines was evaluated, using a commercial human diploid cell vaccine (HDCV) lot as the reference. Two different vaccination regimens, a pre-exposure schedule, and an abbreviated 2-1-1 postexposure schedule (two doses of the vaccine applied bilaterally on day 0, with subsequent single doses given on days 7 and 21) were employed. In both, two of the new vaccines, purified chick embryo cell vaccine and purified Vero rabies vaccine, induced an antibody response equivalent to that of HDCV, while the geometric mean titres for the fetal bovine kidney cell vaccine were somewhat lower. The 2-1-1 regimen, a candidate regimen for economical rabies postexposure treatment, evoked a rapid and high titre antibody response with all four vaccines, peaking on day 14.

Adult↗
PubMed1986

[Serological data on hemorrhagic fever with renal syndrome in Southeast Asia].

Using an indirect immunofluorescent test with Hantaan 76-118 and Puumala strains as antigens, antibodies against haemorrhagic fever with renal syndrome viruses were found for the first time in human in Laos and Viet-Nam and confirmed the previously published results in Thailand. These results suggest the existence of an Hantaan-related virus in these countries.

Antibodies, Viral↗