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Biomedical subjects

P Santos

Publications and source records attributed to P Santos.

At least 19 recordsLinked to original sources
PubMed2003

Alternative inocula as activated sludge surrogate culture for a toxicity test.

The use of activated sludge to assess the potential toxicity and environmental impact of chemicals and wastewaters suffers from several drawbacks related to the heterogeneity, absence of standardization, and health risk associated with this mixed-sewage population. To search for reliable testing inoculum alternatives, the performance of two commercial inocula (BI-CHEM and BIOLEN M112), a garden-soil inoculum and a pure culture of Pseudomonas sp., was compared with an activated sludge inoculum (AS) in the inhibition respiration test ISO 8192-1986 (E). The respiration rates of microbial inocula were assayed for the reference compound 3,5-dichlorophenol. The acute toxicity values (IC(50)) ranged from 6.7 mg/L (Pseudomonas sp.) to 22.7 mg/L (garden soil), overlapping the expected values for activated sludge microorganisms despite the bacterial diversity. The assayed microbial inocula also showed higher reproducibility than AS and an overall similarity of catabolic profiles obtained with Biolog EcoPlates was observed between AS and some mixed inocula. These results point to the potential ability of such inocula as surrogate cultures in relevant activated sludge-based bioassays. New, well-defined, standardized, and safe tools will then be available for monitoring the ecological impact of hazardous substances and effluents, thus providing environmental protection.

Biological Assay↗
PubMed2003

Penile paraffinoma.

A 40-year-old Caucasian man, originating from Ukraine, but living for the last year and a half in Portugal, presented to our department with a one year history of an irregular penile mass and phimosis. He reluctantly admitted that 8 years previously he had multiple mineral oil injections in the penis, for the purpose of penile enlargement. A diagnosis of penile paraffinoma was made and surgical excision was performed at the urology department. Paraffinoma results from mineral-oil injections: such practice is very rare in the western world nowadays, however, it still is performed in many Eastern European Countries.

Adult↗
PubMed2003

Age and gender-related physical activity. A descriptive study in children using accelerometry.

AIM: Precise measures of habitual physical activity are necessary in studies designed to: 1) document the frequency and distribution of physical activity in defined population groups; 2) determine the amount or dose of physical activity requires to influence specific health parameters. The purpose of this study was to document the age and gender-related physical activity levels on a sample of school children. METHODS: The sample for the present study comprised 157 children (boys n=64 and girls n=93), aged 8 to 15 years-old. The CSA activity monitor was used as an objective measure of daily physical activity. Each student in the present study was scheduled to wear the CSA 3 times during the week of monitoring. RESULTS: Boys were involved (p=/<0.05) in more time MVPA than girls. However only in the 11-13 year old group were found significant differences (p=/<0.05). Within gender, significant differences were found out among 11-13 years old (48.7 min) and 14-16 years (72.2 min). The time in MVPA increased across age group in both males and females. Boys participated in more periods of continuous physical activity, bouts of 10 and 20 min respectively, than girls. However no significant differences were found out. CONCLUSION: Our data showed that children under observation, excepted girls in the 11-13 year old group, appear to meet the minimum physical activity level recommended for health. The data also suggested that boys are more active than girls are and that they were significantly more engaged in more time MVPA than girls did. Surprisingly our data showed an increase in MVPA time as age increases. Further studies are needed to compare the different cut off points assessed during daily activity.

Acceleration↗
PubMed2002

Ciclopiroxolamine: in vitro antifungal activity against clinical yeast isolates.

The in vitro susceptibility of 225 clinical isolates of yeasts to ciclopiroxolamine (CPO) was compared with that of clotrimazole, econazole, ketoconazole, miconazole, tioconazole, fluconazole, itraconazole and nystatin using a standardized agar diffusion method (NeoSensitabs). Two hundred and eight strains of yeasts comprising 16 species of Candida and 22 strains belonging to other yeast genera were tested. One strain (0.4%) was resistant, four strains (1.8%) of intermediate susceptibility and 220 strains (97.3%) susceptible to CPO. More strains were susceptible to CPO than to the other antifungals studied. Susceptibility patterns of antifungal agents were not linked to species. The in vitro antifungal susceptibility profile of CPO was better than topical azole derivatives or fluconazole and itraconazole against a wide variety of clinically important yeasts.

Antifungal Agents↗
PubMed2002

Risk factors for nosocomial candidaemia: a case-control study in children.

Candida spp. are increasingly important nosocomial pathogens in critically ill children. Data on risk factors of acquisition in children with candidaemia are limited. To determine the incidence, prognosis and risk factors for acquisition of nosocomial candidaemia in children, a prospective case-control study was performed between July 1998 and January 2000. Candidaemia was defined as the presence of at least one positive blood culture containing Candida spp. For each case, two controls were selected and matched for time and site of hospital admission. Logistic regression was used to obtain estimates of risk after simultaneous control for other variables. Candidaemia was diagnosed in 24 children (1.09/1000 admissions). The species most frequently isolated were Candida albicans (50%) and Candida parapsilosis (17%). Mean age was 58.2+/-58 months between cases and 41.8+/-52.2 months in controls (P not significant). The strongest risk factors for acquisition found in the univariate analysis were underlying disease, previous antibiotic treatment, the number of antibiotics administered, presence of a long-duration catheter and total parenteral nutrition (P<0.05). Sex, the site of hospital admission, artificial ventilatory assistance, transitory venous catheters, arterial and peripherally venous accesses, bladder catheter and transfusions were not statistically significant. The risk factors identified by multiple logistic regression analysis were: permanent catheter (OR 31.5; 3.19-310) and total parenteral nutrition (OR 10.5; 2.76-40.0). Nine (37%) children with nosocomial candidaemia and seven (15%) controls died (P=0.05). These findings should facilitate development of rational approaches to preventing infection and assist clinicians in identifying those patients in whom this life-threatening complication is likely to occur.

Anti-Bacterial Agents↗
PubMed2002

Prevalence of spondyloarthritis in Terceira, Azores: a population based study.

OBJECTIVE: To determine the prevalence of spondyloarthritis (SpA) in the Caucasian population of Terceira Island, Azores. METHODS: Study subjects were recruited from people over the age of 50 years from one half of the island of Terceira (n=24 561). Seventy eight men and 78 women from each five year age group were selected, giving a total of 468 men and 468 women available for study, of whom 490 agreed to take part. These subjects were assessed by dorsal, lumbar, and pelvic radiography. Radiological sacroiliitis was identified in eight patients on whom sacroiliac computed tomography scans were performed. HLA class I typing by polymerase chain reaction with sequence-specific primers was carried out in seven cases. RESULTS: SpA was present in eight subjects (1.6%, 95% CI 0.8 to 2.7%), including seven men (2.7%) and one woman (0.4%). Three (1.2% ) male patients with definite ankylosing spondylitis were HLA-B27 positive. Only one person had a previous diagnosis of SpA. CONCLUSION: These data complement previous studies in European countries on SpA prevalence and establish an estimate of the overall prevalence of SpA in a southern European population.

Adult↗
PubMed2002

[Influence of maternal weight on pregnancy outcome in Cotonou (Benin)].

To evaluate prevalence of obesity in pregnant women in Cotonou and to study the influence of obesity on pregnancy outcome, we analysed retrospectively obstetrical reports of 323 women delivered at the university clinic of gynecology and obstetrics during the year 1999. Prevalence of obesity was 18% among pregnant women and 30.6% of them were overweight. Medical complications of pregnancy were more frequent in obese women than in women with normal weight since obese women showed higher frequency of proteinuria (241% versus 10,5%, p<0.05) and high blood pressure (25.9% versus 4.5%, p<0.001). Diabetes mellitus was only present in obese women with a frequency of 5,2%. Compared with normal weight women, the obese ones showed a tendency to higher frequency of cesarean delivery (34.5% versus 21%), this difference being not statistically significant. Although there was a slightly higher number of reanimated infants in obese women, the difference was not significant. Six percent hypotrophic infants were observed in obese mothers versus 13.1% in mothers with normal weight (non significant) but it can be noticed that lean mothers showed statistically higher frequency of hypotrophic infants than normal weight mothers (33.3% versus 13.1%, p<0.01). This study shows that obesity in women represents, even in developing country, an obstetrical risk factor leading to high frequency of complications during pregnancy and needs the development of preventive actions.

Adult↗
PubMed2001

Atypical skin lesions caused by Curvularia sp. and Pseudallescheria boydii in two patients after allogeneic bone marrow transplantation.

We report two patients who developed atypical skin lesions caused by Curvularia sp. and Pseudallescheria boydii after allogeneic bone marrow transplantation for severe aplastic anemia. The first patient (female, 18-year-old) had multiple hemorrhagic vesicles on day +30 after her second BMT for graft failure. Pseudallescheria boydii was isolated from a skin biopsy. The patient died of respiratory failure probably as a consequence of systemic fungal infection. The second patient (male, 9-year-old) developed an ecthyma gangrenosum-like lesion on his right palm on day +8. Curvularia sp. was isolated from a skin biopsy. Liposomal amphotericin was given to achieve a total dose of 30 mg/kg and followed by oral itraconazole until steroids were discontinued. The infection resolved completely and the patient has remained disease-free. We conclude that emerging fungal organisms such as those described in this report are increasingly recognized in this setting. Early recognition and biopsy of these cutaneous lesions will allow prompt initiation of therapy to prevent systemic infection.

Adolescent↗
PubMed2001

Parenteral nutrition and cyclosporine: do lipids make a difference? A prospective randomized crossover trial.

AIMS: This prospective, controlled, randomized crossover trial was conducted to assess the effects of parenteral nutrition, with or without lipids, in cyclosporine (CyA) pharmacokinetics. METHODS: 10 adult patients were randomized on the day of allogeneic bone marrow transplantation to receive isocaloric and isonitrogenous parenteral nutrition admixtures without (regimen A) or with lipids (regimen B). Admixtures were started on average by day + 7.4; 5 patients received regimen A followed by B, 5 in reverse order. Blood samples were collected at day 4 after transplantation, under oral diet, and 4 days after the initiation of each regimen as the sole nutrition support. At each time point, 8 whole blood samples were analysed for CyA to evaluate: area under the curve (AUC), trough concentration and systemic clearance. Clinical/laboratory events were recorded until 31 months of follow-up. RESULTS: There was no evidence of a period or treatment-by period interaction, thus results were combined for further analysis. There were no statistically significant differences between regimens in any CyA pharmacokinetic parameters; there were no significant differences from baseline values, except for a higher systemic clearance of CyA with regimen A (0.40+/-0.09 vs 0.29+/-0.06 L/Kg/h, p=0.03). CONCLUSIONS: The provision of 0.8 g/Kg/d of a 50:50 mixture of medium and long chain triglycerides did not affect CyA parameters, which were closer to baseline. In the short or long term there were no attributable side effects.

Adult↗
PubMed2001

Comparative hemodynamic effects of urapidil and labetalol after electroconvulsive therapy.

Urapidil, a postsynaptic alpha 1 -adrenergic antagonist, has been reported to improve intraoperative hemodynamic stability, although it has never been used to prevent the hemodynamic response of electroconvulsive therapy (ECT). This study was designed to evaluate the clinical effectiveness of urapidil, as an alternative to labetalol, in preventing the hemodynamic response of ECT. Twenty-seven patients undergoing a series of six consecutive ECT treatments were studied. Each patient received all three pretreatments twice: no drug, labetalol 0.2 mg/kg, or urapidil 25 mg. Systolic, diastolic, and mean blood pressure and heart rate (HR) were recorded during the awake state, after anesthesia induction, and 1, 2, 5, 10 and 30 minutes after electroencephalographic (EEG) seizure ended. The duration of the EEG convulsion was also recorded. After induction, the HR increased for no drug and urapidil pretreatments, whereas it decreased when labetalol was given. Labetalol and urapidil attenuated the peak increase of blood pressure and returned it to earlier baseline values. There were no differences in the duration of EEG convulsion between the three pretreatments. Urapidil seems to be a good alternative to labetalol for attenuating the hypertensive response to ECT in cases where there is a contraindication to beta-antagonists.

Adrenergic alpha-Antagonists↗
PubMed2001

[Activity of itraconazole against clinical isolates of Aspergillus spp. and Fusarium spp. determined by the M38-P NCCLS method].

The antifungal activity of itraconazole was studied in 101 clinical isolates of Aspergillus fumigatus, A. flavus, A. niger, A. terreus, A. nidulans, A. candidus, A. glaucus, A. clavatus, Fusarium solani, F. oxysporum and F. semitectum. The minimum inhibitory concentrations (MIC) were determined according to the protocol of the M38-P National Committee for Laboratory Standards (NCCLS) document using a microdilution method in 1640 RPMI liquid medium (visual reading at 48 and 72 h incubation). In general, the MIC did not vary with time of incubation, except in a Z. fumigatus strain in which the MIC went from 2 to 16 mg/l. The geometric mean of the MIC and MIC(90) of itraconazole for Aspergillus spp. was 0.44 mg/l and 0.5 mg/l, respectively; and for Fusarium spp. it was 14.1 mg/l and 16 mg/l, respectively. With 0.5 mg/l 75% of the Aspergillus spp. strains were inhibited, and 100% of these strains were inhibited with 2 mg/l. A. niger and A. fumigatus were the most resistant species (MIC(90) 2 mg/l). The MIC of all the Fusarium strains essayed was between 4 and 16 mg/l.

Antifungal Agents↗
PubMed2000

Airway ignition during CO2 laser laryngeal surgery and high frequency jet ventilation.

We present a case of a patient submitted for extirpation of a neoplasm of the larynx, by means of carbon dioxide laser surgery. High frequency jet ventilation was applied by means of orotracheal intubation with two Teflon catheters, 2 mm in external diameter and 30 cm in length, attached with three equally placed strips of adhesive paper tape. One catheter was used to inject the jet volume and the other used to measure the airway pressure. The adhesive strips were moistened and FiO2 was lower than 50%. After 30 min using the laser, an airway fire was noticed. Ventilation was interrupted and the catheters were removed. The patient was reintubated with an endotracheal tube of 6 mm ID and the surgical procedure was continued until the tumour was removed. Two factors contributed to the airway fire: the ignition of the lowest adhesive strip that had dried and the use of the laser in the mode of continuous pulsation.

Fires↗
PubMed2000

Women's reports of severe (near-miss) obstetric complications in Benin.

This study examines the validity of a survey instrument on near-miss obstetric complications. Three groups of women--with severe complications, with mild complications, and with a normal delivery--were identified retrospectively in three hospitals in South Benin and interviewed at home. The concept of "near-miss" was used to identify women with severe episodes of morbidity. The questionnaire was able to detect, with some accuracy, eclamptic fits, abnormal bleeding in the third trimester for a recall period of at least three to four years, and all episodes of bleeding independent of timing within a period of two years. Questions concerning dystocia and infections of the genital tract generated disappointing results except when information on treatment was included. Overall, better results were achieved for antepartum and acute events. Severity made a positive difference only in the case of eclampsia, with an increase in sensitivity. The implications of the results for using women's recall of obstetric complications in surveys are discussed.

Benin↗
PubMed1999

[Determination of the in vitro antifungal susceptibility of clinically important yeasts using the Sensititre system].

Using Sensititre (AccuMed, USA) we studied the in vitro antifungal activity of amphotericin B, fluconazole, itraconazole, ketoconazole and 5-fluorocytosine against 250 clinical yeast isolates taken from different hospitals, including Candida (151 C. albicans, 15 C. krusei, 14 C. parapsilosis, 11 C. tropicalis, 10 C. glabrata, 4 C. guilliermondii, 3 C. rugosa, 2 C. viswanathii, 2 C. famata and 2 C. kefyr), Cryptococcus (32 C. neoformans and 1 C. laurentii), Trichosporon (2 isolates) and Rhodotorula rubra (1 isolate). All the strains were susceptible to amphotericin B and showed an MIC <1 mg/l. The susceptibility of C. albicans (MIC(90) <256 mg/l), C. krusei (MIC(90) <64 mg/l), C. glabrata (MIC(90) <64 mg/l) and C. neoformans (MIC(90) 32 mg/l) to fluconazole was lower (14% isolates being resistant and 16.8% susceptible depending on the dose). The largest number of strains resistant to itraconazole was observed in C. albicans and C. glabrata (17.2% resistant and 24% susceptible and susceptible depending on the dose, respectively). Ketoconazole and 5-fluorocytosine were not effective in vitro against 12.8% and 2%, respectively, of all the isolates studied. Nine C. krusei and seven C. neoformans (12.9%) showed dose-dependent susceptibility to 5-fluorocytosine.

Amphotericin B↗