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Biomedical subjects

P Ruck

Publications and source records attributed to P Ruck.

At least 91 records · Page 5Linked to original sources
PubMed1993

Aberrant expression of macrophage-associated antigens (CD68 and Ki-M1P) by Schwann cells in reactive and neoplastic neural tissue. Light- and electron-microscopic findings.

The monoclonal antibodies KP1 (CD68), PG-M1 (CD68), and Ki-M1P can be used to detect normal and neoplastic monocytes/macrophages in formalin-fixed, paraffin-embedded tissue. However, systematic investigations undertaken on various tissues have revealed that reactivity with these antibodies is also found in a few cells that do not belong to the mononuclear phagocyte system. The immunoreactivity of normal, reactively altered, and neoplastic Schwann cells with these antibodies was investigated using intact peripheral myelinated nerves, nerves exhibiting Wallerian degeneration, traumatic neuromas, appendixes with neurogenic appendicopathy, granular cell tumors, neurofibromas, and neurogenic sarcomas. The results obtained by light microscopy showed that Schwann cells of nerves with Wallerian degeneration and those in traumatic neuroma, neurofibroma, and granular cell tumor exhibit intracytoplasmic immunoreactivity, which is usually intense, with KP1, Ki-M1P, and PG-M1, but normal myelinated nerves, neurogenic sarcoma, and Schwann cells in neurogenic appendicopathy do not react with these antibodies. No Schwann cells were stained by MAC387 or anti-lysozyme. The site of immunoreactivity with these antibodies was also investigated by electron microscopy. One of the granular cell tumors and macrophages in lymphoid tissue were investigated by the immunogold technique using both pre- and postembedding methods. In granular cell tumor the reaction product was located in phagolysosomes; in macrophages it was found in phagosomes and/or lysosome-like granules. Our findings therefore indicate that immunoreactivity with KP1, Ki-M1P, and PG-M1 can also be expected in cells that do not belong to the mononuclear phagocyte system if they exhibit phagocytosis and/or autophagy.

Antigens, CD↗
PubMed1992

Filiform polyposis: a case report describing clinical, morphological, and immunohistochemical findings.

Filiform polyposis (FP) is a rare condition of uncertain pathogenesis, 28 cases of which have been published since it was first described in 1965. It is usually found in association with chronic inflammatory bowel disease, especially Crohn's disease and ulcerative colitis. The condition is characterized by the presence of numerous, densely packed, filiform polyps in the colon, which may resemble villous adenomas on endoscopy. We describe a case of FP occurring in a 33-year-old man with a 5-year history of Crohn's disease, in whom subtotal colectomy was performed because of perforation of the sigmoid colon. Microscopy revealed inflammatory pseudopolyps covered by largely normal and non-dysplastic colonic epithelium. The neuroendocrine system of the intestine in FP was investigated for the first time in this case: marked hyperplasia of endocrine cells immunoreactive for serotonin, somatostatin and enteroglucagon and of neural structures immunoreactive for substance P and vasoactive intestinal peptide was noted in the polyps and the adjacent intestinal mucosa. The patient has experienced no further complications in the 12 months since the operation. Medication administered in FP depends mainly on the nature of the underlying disease, and the amount of information published about this condition is as yet insufficient to allow any one specific type of treatment to be recommended. FP alone is not an indication for bowel resection but complications, such as massive haemorrhage or intestinal obstruction, may necessitate surgical intervention.

Adult↗
PubMed1992

Extracellular matrix in hepatoblastoma: an immunohistochemical investigation.

Eleven hepatoblastomas of various subtypes and normal liver tissue were investigated with antibodies against collagen types I-VI, laminin, fibronectin and endothelial and macrophage-associated antigens. Epithelial hepatoblastoma cells, unlike non-neoplastic hepatocytes, exhibited intracellular immunoreactivity for various extracellular matrix proteins (depending on the subtype: laminin, fibronectin and collagen types III, IV and V). The intracellular expression of extracellular matrix proteins by the tumour cells increased from the fetal subtype, through the embryonal subtype, to the small cell subtype. The epithelial tumours exhibited sinusoid-like blood vessels in numbers that varied according to the subtype. These contained Kupffer cells and exhibited greater amounts of the basement membrane components collagen type IV and laminin in the perisinusoidal space than those in the normal liver. The small cell hepatoblastoma exhibited smaller numbers of sinusoids, pronounced intracellular expression of extracellular matrix proteins and large numbers of fibres immunoreactive for collagen type III. In the mixed hepatoblastomas, the extracellular matrix of the osteoid was most strongly immunoreactive for collagen type I and that of the spindle cell areas for collagen type III.

Carcinoma, Hepatocellular↗
PubMed1992

The decidua of early human pregnancy: immunohistochemistry and function of immunocompetent cells.

Like the endometrial stroma, the decidua contains lymphoreticular cells, and these are probably involved in immunological interactions between the conceptus and the mother. Lymphoreticular cells in decidual tissue obtained from 12 patients undergoing therapeutic abortion of an intact pregnancy at 6-10 weeks' gestation were investigated in this study. Immunophenotyping with a broad panel of monoclonal antibodies revealed various subpopulations of lymphoreticular cells. Macrophages (Ki-M6+, Ki-M7+, Ki-M8+, KP1+, MAC 387+ and Ki-M1P+) represented the largest fraction of intradecidual lymphoreticular cells. CD3+ and CD8+ lymphocytes were found in moderate numbers and CD4+ cells in small numbers. The majority of the intradecidual lymphoid cells exhibited an unusual phenotype [CD7+, CD2+, CD56+, triple negative (CD3-, CD4-, CD8)]. The distribution of these unusual lymphocytes mirrored that of the so-called endometrial stromal granulocytes. A few of these stromal granulocytes reacted with the macrophage-associated antibody KP1, but not with Ki-M1P, another macrophage marker. This was confirmed by immuno-electron microscopy. The finding that intradecidual CD3+ lymphocytes express neither the alpha/beta nor the gamma/delta heterodimer of the T cell antigen receptor was unexpected. However, these cells did express the alpha/beta heterodimer after in vitro culture with PHA-P and recombinant exogenous interleukin-2. No stimulated T lymphocytes expressing activation antigens could be detected. B lymphocytes, T and B immune accessory cells and CD15+ granulocytes were found only in small numbers or were absent. Amongst cells expressing NK cell markers, CD57+ and CD16+ cells were found in small to moderate numbers, while CD56+ cells were detected in large numbers.(ABSTRACT TRUNCATED AT 250 WORDS)

Antibodies, Monoclonal↗
PubMed1991

Application of an anti-myosin antibody for scintigraphic differential-diagnosis of infantile tumors.

Immunoscans with the Fab-fragment of the monoclonal anti-myosin antibody R11D10 labeled with 111In showed significant uptake in rhabdomyosarcoma, rhabdoid tumor, and primitive neuroectodermal tumor. The diagnosis of an antimyosin positive or negative tumor with the aid of the immunoscan was superior to clinical findings in combination with ultrasound and CT, and to histological diagnosis in tumor biopsies. In addition chemotherapeutically induced renal impairment can be diagnosed by diminished renal uptake of antimyosin.

Adolescent↗
PubMed1991

Detection of infection in postoperative orthopedic patients with technetium-99m-labeled monoclonal antibodies against granulocytes.

A prospective study of 106 orthopedic patients was performed for the detection of infection in the early postoperative stage using 99mTc-labeled murine Mabs directed against epitopes on granulocytes. Accuracy was 81% in the hips (n = 26), 81% in the thigh (n = 21), 84% in the knee (n = 19), and 100% in the tibia (n = 27). The technique did not work well in the spine where false-negative results were observed in the three patients studied. One patient suffered transient swelling of the eyelids following injection. Optimal imaging results were obtained 2-6 hr postinjection.

Adolescent↗
PubMed1990

[Carcinoid tumor of the middle ear. Morphology, immunohistochemistry, clinical aspects and differential diagnosis].

We describe the unusual case of a carcinoid tumour of the middle ear occurring in a woman of 50 years of age. The tumour exhibited both glandular and neuroendocrine differentiation. Immunohistochemical demonstration of chromogranin A is of particular diagnostic importance as evidence of neuroendocrine differentiation. The morphological spectrum of the middle ear tumours with neuroendocrine and/or glandular differentiation reported in the literature is discussed in detail. These tumours are arranged schematically according to the degree of glandular and of neuroendocrine differentiation, and are compared with the tumours of other regions. The data in the literature do not allow unequivocal assertions concerning the prognosis of carcinoid tumours of the middle ear to be made, and very diverse therapeutic measures have been employed in the reported cases. In our case there is no evidence of recurrence six months after local tumour excision.

Biomarkers, Tumor↗
PubMed1990

Neuroendocrine differentiation in hepatoblastoma. An immunohistochemical investigation.

Hepatoblastoma exhibits a wide range of epithelial and mesenchymal lines of differentiation. Neuroendocrine differentiation in this tumor has not previously been reported. We investigated seven hepatoblastomas of different subtypes (five pure epithelial hepatoblastomas, including one small-cell hepatoblastoma, and two mixed hepatoblastomas) using a broad panel of antibodies against epithelial, mesenchymal, neural, and neuroendocrine markers, alpha-1-antitrypsin (alpha 1-AT), alpha-1-antichymotrypsin (alpha 1-ACT), alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), serotonin, and 14 regulatory peptides. Chromogranin A-immunoreactive neuroendocrine tumor cells, some of which also exhibited immunoreactivity for serotonin and somatostatin, were found in the fetal and embryonal parts of the mixed hepatoblastomas. The osteoid-like material in the mixed hepatoblastomas contained cells with immunoreactivity for chromogranin A, neuron-specific enolase, keratin, and alpha 1-AT, alpha 1-ACT, AFP, and CEA, in addition to S-100 protein and vimentin. Parallels to the neuroendocrine differentiation in hepatoblastomas are found in tumors of the gastrointestinal tract and bronchopulmonary tree. These tumors may also exhibit a neuroendocrine component; that is, multidirectional differentiation may occur, as in hepatoblastoma. The immunoreactivity of some of the cells of the osteoid-like material for keratin, alpha 1-AT, alpha 1-ACT, AFP, CEA, and chromogranin A suggests that these cells--and probably the surrounding material--are of epithelial origin.

Antibodies↗
PubMed1990

Intradecidual T lymphocytes lack immunohistochemically detectable T-cell receptors.

The decidua apparently plays a major role in immune tolerance to the semiallogeneic embryo. In the present study we applied the immunohistochemical ABC method to investigate the expression of CD3, TCR alpha/beta and TCR gamma/delta molecules on intradecidual T cells with a panel of mAbs. Whereas intradecidual T cells expressed readily detectable amounts of CD3, they lacked immunohistochemically detectable amounts of either alpha/beta or gamma/delta TCR heterodimers on their cell surface. Peripheral blood smears of the same patients showed normal expression of alpha/beta and gamma/delta TCR on CD3+ T cells. The specific absence of (alpha/beta or gamma/delta) TCR molecules on the surface of intradecidual T cells could be one of the decisive factors in tolerance of the maternal immune system towards the fetus.

Cells, Cultured↗
PubMed1990

Immunoreactivity of human tissue mast cells: nonspecific binding of primary antibodies against regulatory peptides by ionic linkage.

Tissue mast cells (TMC) are known to react with antibodies against various regulatory peptides (RP). The specificity of such reactions was investigated by various methods in this study. When normal immunohistochemical staining procedures were employed. TMC in the vermiform appendix and in a cutaneous mastocytoma reacted with antibodies against ACTH, Leu-enkephalin, Met-enkephalin, and peptide histidine isoleucine (PHI). Antibody specificity was tested by absorption controls, and staining specificity by varying the concentration of the primary antibodies and the pH and sodium chloride concentration of the buffer used for rinsing and diluting. In absorption controls, staining of the TMC by anti-PHI was diminished but staining by anti-ACTH, anti-Leu-enkephalin, and anti-Met-enkephalin remained unchanged. Unlike control reactions, immunostaining of TMC with antibodies against RP exhibited marked dependence on antibody concentration and the pH and sodium chloride concentration of the buffer. Alkalization of the buffer led to an obvious increase in the reaction with antibodies against RP, and lowering the pH to 6.0 usually resulted in abolition of the reaction. These results indicate that the immunostaining of TMC with antibodies against RP, including PHI, was nonspecific. It is postulated that the granules of TMC bind certain antibodies by a cation-exchange mechanism involving ionic interactions with positively charged groups in the F(ab')2 and/or Fc segments.

Antibodies↗
PubMed1989

Carcinoid tumour of the middle ear. A morphological and immunohistochemical study with comments on histogenesis and differential diagnosis.

Primary carcinoid tumours of the middle ear are extremely rare, only nine cases having been reported. However, their true incidence is probably greater, since they are very difficult or impossible to distinguish from adenomas and adenocarcinomas with conventional histological stains. We describe the clinical, histological, immunohistochemical and ultrastructural findings in a carcinoid tumour of the middle ear in a 50-year-old woman. Immunohistochemical studies on non-neoplastic middle ear mucosa undertaken to investigate the histogenesis of such tumours are also reported. Histologically, the tumour consisted of both solid areas and areas of tubular structures containing intraluminal mucus. All the tumour cells reacted with the anti-keratin antibody KL 1; some were argyrophil and reacted with antibodies against neuron-specific enolase, chromogranin A, Leu-7, serotonin, pancreatic polypeptide, glucagon and lysozyme. Electron microscopy revealed dense core granules in the tumour cells. Endocrine cells could not be detected in non-neoplastic middle ear mucosa. Pancreatic-polypeptide-like immunoreactivity was demonstrated immunohistochemically in all three other published cases of carcinoid tumour of the middle ear investigated for this peptide, and glucagon-like immunoreactivity was also exhibited by one of these. Since carcinoid tumours of the middle ear often, as in this case, exhibit some degree of glandular differentiation, immunohistochemical or electron-microscopic investigation to detect neuroendocrine differentiation is of particular importance in adenomatous middle ear neoplasms.

Carcinoma↗