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Biomedical subjects

P Probst

Publications and source records attributed to P Probst.

At least 217 records · Page 12Linked to original sources
PubMed1971

High-yield method for dispersing simian kidneys for cell cultures.

A technique for dispersion of animal tissue cells is described. The proposed technique is based on the concomitant use of trypsin and disodium ethylenediamine tetraacetate (EDTA). The use of the two dispersing agents (trypsin and disodium EDTA) markedly enhances cell yield as compared with the standard cell dispersion methods. Moreover, significant reduction in the amount of time required for complete tissue dispersal, presence of a very low number of nonviable cells, less cell clumping, and more uniform monolayer formation upon cultivation compare favorably with the results usually obtained with the standard trypsinization technique.

Animals↗
PubMedDate not supplied

Renal artery stenosis and evaluation of the effect of endoluminal dilatation. Comparison of dynamic CT scanning and I-131-OIHA renogram.

Percutaneous transluminal dilatation was performed in 16 patients with hypertension and renal artery stenoses. Dynamic CT scanning and an I-131-OIHA renogram was performed before and after dilatation. After the procedure (follow-up greater than or equal to 3 months) the hypertension was assessed clinically as normalized in six, improved in five, and unimproved in five patients. The attenuation difference at dynamic CT scanning between both kidneys at the time of peak enhancement following intravenous bolus injection of contrast material diminished significantly from 16 +/- 4 HU before to 7 +/- 6 HU (P = 0.015) after dilatation in the patient group with cured hypertension, whereas the relative opacification of the poststenotic kidneys increased from 43.6 +/- 2.4% to 47.4 +/- 1.7% (P = 0.014). The results of the Isotope renogram showed a similar increase of the relative function of the poststenotic kidneys from 33 +/- 5% to 38 +/- 7% (P = 0.227) and significant improvement of the clearance from 112 +/- 34 to 175 +/- 48 ml/min/1.73m2 (P = 0.040). The results of both techniques did not significantly change in the group of patients with only moderate improvement of hypertension. Deterioration of the results occurred in those patients in which the hypertension remained unchanged.

Adult↗
PubMedDate not supplied

Experimental acute renal artery stenosis. Dynamic CT and renal perfusion.

Unilateral renal artery stenosis of varying degrees was induced in six of seven pigs. Renal perfusion prior to and after stenosing the renal artery was determined by standard renal clearance and by the direct measurement of renal blood flow. Renal blood flow was reduced from 29 to 56% (mean 40%) in the poststenotic ischemic kidneys. A significant correlation was found between the opacification of the poststenotic ischemic kidneys after intraaortic bolus injection and direct renal blood flow (r = 0.89, P less than 0.01). There was also a significant correlation (r = 0.85, P less than 0.01) between the opacification of the kidneys and their respective glomerular filtration rates. Renal opacification determined by CT correlates significantly with renal perfusion.

Acute Disease↗
PubMedDate not supplied

Functional assessment of coronary arteries by poststenotic intravascular Doppler ultrasound.

This study sought to delineate the impact of the rate pressure product on intraluminal Doppler velocity measurements and to determine the relation between poststenotic vasodilator reserve and percent luminal obstruction in coronary vessels. Twenty patients with single-vessel coronary disease were studied prior to coronary angioplasty and at follow-up 6 months later. Intracoronary velocity reserve after administration of adenosine was measured distal to the stenosis with a Doppler-tipped guide wire and was compared to quantitative coronary angiography and adenosine myocardial perfusion scintigraphy. The rate pressure product was confirmed as significant covariate (ANCOVA, p < 0.005) of intracoronary Doppler reserve. When normalized to rate pressure product, poststenotic Doppler velocity reserve in stenosed arteries was significantly lower than in patent arteries as classified by quantitative coronary angiography (1.7 +/- 0.6 vs. 2.9 +/- 0.5, p < 0.001) and perfusion scintigraphy (1.5 +/- 0.4 vs. 2.8 +/- 0.5, p < 0.001). Normalized Doppler velocity reserve showed a nonlinear but highly significant relation to percent area stenosis [y = 3.0.(1 - exp[0.081 (x - 100)]), p < 0.001]. When normalized Doppler velocity reserve was less than 2.0, coronary disease was identified with 95% specificity and 94% sensitivity in comparison to perfusion scintigraphy. Thus, in coronary arteries poststenotic Doppler reserve and percent area stenosis show a significant nonlinear relation. Doppler velocity reserve when normalized to rate pressure product can be used to characterize the hemodynamic impact of coronary obstructions.

Adenosine↗