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Biomedical subjects

P Palma

Publications and source records attributed to P Palma.

At least 55 records · Page 3Linked to original sources
PubMed1992

Assessment of hepatic phagocytic activity by in vivo microscopy after liver transplantation in the rat.

Phagocytic activity of sinusoidal lining cells was studied in 32 livers of male Lewis rats by in vivo fluorescence microscopy with epiillumination. Normal livers (group 1, n = 8) were compared with orthotopic syngeneic liver grafts 90 min after reperfusion after a period of cold storage in University of Wisconsin solution for 17 hr (group 2, n = 10) or 24 hr (group 3, n = 14). After bolus injection of fluorescence-labeled latex particles (3 x 10(8)/kg; diameter = 1.1 micron), zonal distribution and kinetics of adherence of latex beads were quantified by off-line video analysis. Hepatocellular function was estimated by the rate of bile production and biliary concentrations of bile acids. In normal livers 50%, 37% and 13% of injected latex beads adhered in zones 1, 2 and 3, respectively, whereas in transplanted livers a more homogeneous distribution was found (group 2: 37%, 41%, 22%; group 3: 37%, 39%, 24%; p less than 0.01 vs. controls by analysis of variance). Kinetic analysis of phagocytic activity showed no significant difference between group 1 (88% adherence of visible latex beads 3 min after injection) and group 2 (90% adherence). However, after long-term preservation in group 3, sinusoidal adherence was significantly faster (96.4% adherence; p less than 0.001). Bile secretion in group 2 was lower than in controls and severely depressed in group 3 (group 1: 1.1 +/- 0.07 microliter/min/gm liver [mean +/- S.E.M.]; group 2: 0.8 +/- 0.07; group 3: 0.1 +/- 0.04; p less than 0.001) without significant changes in bile acid concentrations.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenosine↗
PubMed1989

A new technique for functional surgery of the nasal valve area.

Nasal valvular obstruction is a neglected and disregarded clinical entity in current practice. Rhinomanometry and nasal endoscopy allow a better evaluation of the degree and the site of the stenosis. The distinction between nasal valve and nasal valve area is fundamental in surgical practice. Valvular disturbances are often induced or aggravated by rhinoplastic procedures due to scar tissue formation in the region of the nasal valve area and/or to excessive resection of supporting cartilaginous structures. Routine septal surgery is often followed by sequelae in the valve area structures. The treatment of valvular disturbances is basically surgical. A systematic surgical approach should comprise the structures of the nasal valve area as well as the structures functionally related to it. After a brief review of the techniques described in the literature, the authors present an original corrective technique for the valve area deformities, performed through the hemitransfixion incision. This technique results from previous experience founded on Cottle's surgical philosophy. This technique assures the correction of the valve area deformities as well as the performance of surgical manoeuvres extended to the osteocartilaginous pyramid and the nasal cavities. It is therefore possible to properly correct the areas of resistance and to re-establish adequate functional relationships between the various parts.

Cartilage↗
PubMed1989

[The nasal valve area: structure, function, clinical aspects and treatment. Sulsenti's technic for correction of valve deformities].

The nasal valve and nasal valve area are two entities which should not be confused. The nasal valve area is the narrowest portion of the nasal passage. It is bounded: medially by the septum; superiorly and laterally by the caudal margin of the upper lateral cartilage and its fibro-adipose attachment to the pyriform aperture ('empty triangle'); inferiorly by the floor of the pyriform aperture. The nasal valve, on the other hand, is the specific slit-like segment between the caudal margin of the upper lateral cartilage and the septum. From a physiological and surgical point of view, this distinction is fundamental. The nasal valve area is the site of the highest nasal resistance. Therefore, small deformities of the valve area may severely impair the dynamics of nasal air flow. Rhinomanometry and nasal endoscopy permit the best definition of valve pathophysiology. After having discussed the various surgical techniques reported in the literature, the authors present an original technique for the surgical correction of valvular deformities. It is completely performed through Cottle's hemitransfixion incision. The technique has several advantages: a) performance of only one incision, sufficient to visualize the entire nasal valve and cartilaginous vault, thus minimizing the risk of scar tissue formation; b) through the space thus created it is possible not only to correct the entire septum, but also to inspect and easily reach the structures constituting the nasal valve area; c) it is possible to use various types of grafts to support or reconstruct the valve area; d) it is possible to reach the key area as well as to do lateral osteotomies: all variations in shape and position of the nasal pyramid may be performed in order to normalize direction and pressures of nasal air flow: e) through the retrograde undermining of the lower lateral cartilages the resistance of the cul-de-sacs may be optimally adjusted; f) it is possible to change the shape, size and position of the nostrils as well as to properly correct the columella and naso-labial angle. The technique, therefore, permits maximum correction of valvular deformities with the concomitant functional modification of any abnormalities of the nasal pyramid.

Adult↗
PubMed1985

Simultaneous extraction and reverse-phase high-performance liquid chromatographic determination of adenine and pyridine nucleotides in human red blood cells.

A simple and rapid method for the determination of ATP, ADP, AMP, NADP+, NAD+, NADPH, and NADH in human erythrocytes is described. A single-step extraction procedure employing alkaline medium and CF 50A Amicon ultrafiltration membranes allows a simultaneous and total recovery of the compounds of interest. Analysis is performed by reverse-phase high-performance liquid chromatography on a 5-micron Supelcosil LC-18 column and uv detection. Extraction and analysis require about 30 min. Levels of adenine and pyridine nucleotides in normal adults are also presented.

Adenine Nucleotides↗