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Biomedical subjects

P Neri

Publications and source records attributed to P Neri.

At least 145 records · Page 8Linked to original sources
PubMedDate not supplied

Biphasic anaphylactic reaction to Ketorolac tromethamine.

Ketorolac tromethamine is a recent injectable non-steroidal anti-inflammatory drug (NSAID) with analgesic properties approved for short-term pain management. In spite of its increasing use both in adults and children, relatively few allergic-like reactions have been reported. Reactions are often severe, and a death occurred following an intramuscular injection of ketorolac.

Adolescent↗
PubMedDate not supplied

Hereditary hemochromatosis masquerading as rheumatoid arthritis.

Early erroneous diagnosis of rheumatic disease is common in subjects with arthropathy due to hereditary hemochromatosis. A 71-year-old male with chronic obstructive pulmonary disease and monoclonal gammopathy underwent hip replacement and was referred to our Department because of altered liver function tests. Test results were negative for hepatitis B surface antigen and hepatitis C virus, and positive for rheumatoid factor. A diagnosis of rheumatoid arthritis had been made on the basis of compatible joint involvement and laboratory data and steroid treatment prescribed. Since his serum ferritin was 3249 ng/mL, genetic testing for hereditary hemochromatosis was carried out and revealed homozygosity for Cys282Tyr mutation in the HFE gene. Liver biopsy disclosed cirrhosis compatible with hemochromatosis. Following a review of the patients' radiographs, the diagnosis of hemochromatosis arthropathy was made. Phlebotomies and family screening for hereditary hemochromatosis were done. The most logical explanation for the positive rheumatoid factor result in this subject are his age and the presence of two chronic diseases involving long-standing antigenic stimulation and monoclonal gammopathy of uncertain significance. It is important to distinguish rheumatoid arthritis from hemochromatosis arthropathy for several reasons: patients with hereditary hemochromatosis do not require corticosteroid treatment; in case of erroneous diagnosis of rheumatoid arthritis, phlebotomy is not started early, and familial genetic counseling is not considered. In male subjects with positive rheumatoid factor and joint and liver disease, hereditary hemochromatosis should be considered. More liberal use of genetic testing is justified in such cases.

Aged↗
PubMedDate not supplied

[Microbiological characterization of solid wastes and aerosol in a municipal solid waste landfill site].

Authors report data about microbiological characterization of solid wastes and aerosol in the municipal solid waste landfill site in Poiatica (RE). In solid waste samples high values of total coliform and fecal coliform were observed (10(5)-10(6) CFU/g): total bacterial counts at 22 degrees, at 36 degrees and at 44 degrees ranged from 10(7) to 10(9) CFU/g. Aerosol samples collected during waste movement in the landfill site showed values of total and fecal bacterial ranging from 10(3)-10(4) CFU/m3. Staphylococci and fungi reported the same values while streptococci, total and fecal coliform and spore evidenced lower values. Municipal solid wastes and aerosol have to be considered as an infective substrate: it is necessary to adopt protective barriers in occupationally exposed subjects.

Aerosols↗
PubMedDate not supplied

[Satisfaction and critical aspects of inpatients care among patients with psoriasis].

OBJECTIVES: To evaluate satisfaction with care for psoriatic inpatients. Identify areas in need of interventions and actions to improve the satisfaction with care. METHODS: We analyzed 133 patients with psoriasis and 335 patients affected by other dermatological diseases in their first access at the hospital. Six determinants of satisfaction with care were investigated with a self administered questionnaire. Moreover the overall satisfaction was documented. Descriptive analysis and a multiple logistic regression analysis correcting for confounding factors were performed. Focus groups were conducted with patients to further investigate about specific aspects of relationships between patients and personnel. RESULTS: Psoriatic inpatients were less satisfied than other dermatological inpatients regarding Coordination of care, Information and education, Emotional support. Emotional support was the determinant with the smallest percentage of satisfied patients. Focus groups confirmed what was identified with the questionnaire and suggested necessary interventions. CONCLUSIONS: It is necessary to improve the communication skills of health personnel. Information for patients need to be enhanced and the management of psoriatic patients has to be improved.

Adult↗
PubMedDate not supplied

Reversible binding of vibrio cholerae toxin on NAD-sepharose.

When a crude or partially purified culture of Vibrio Cholerae is passed through a column of NAD-Sepharose, the skin toxic material is bound to the gel. Choleragen may be recovered by increasing the ionic strength of the elution buffer, as shown by the skin test, immunodiffusion against anti-choleragen antiserum and polyacrylamide gel electrophoresis. Affinity chromatography on NAD-Sepharose may be helpful for the concentration and purification of Choleragen.

Animals↗
PubMedDate not supplied

Micro-determination of amino acid composition of proteins electroblotted onto polyvinylidene difluoride membranes.

A method for determination of amino acid composition of proteins separated by SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) membranes is described. A single blotted band containing 50 to 200 pmoles of protein was cut out and submitted to acid hydrolysis with HCl followed by derivatization with phenylisothiocyanate. The amino acid derivatives were separated by reverse phase high-performance liquid chromatography. Bovine serum albumin, lysozyme, myoglobin, ovalbumin, soybean trypsin inhibitor and carbonic anhydrase were analyzed; the results revealed a good correspondence with reported values. This can be considered an analytical method to determine the amino acid composition of samples from microquantities of protein mixtures, particularly in those cases in which SDS-polyacrylamide gel electrophoresis is the most suitable separation system.

Amino Acids↗
PubMedDate not supplied

Different interactions of human and bovine serum albumin with Cibacron Blue and Blue Dextran.

The interaction of human and bovine serum albumin with Cibacron Blue and Blue Dextran in aqueous solution was studied by means of difference spectroscopy. Both human and bovine albumin interact strongly with underivatized Cibacron Blue in three independent binding sites (K = 105). On the contrary, Blue Dextran interacts strongly only with human albumin, but does not bind appreciably to bovine albumin. These results suggest that the binding sites are exposed and easily accessible in human albumin, while in bovine albumin they are sterically hindered and therefore not accessible to the bulky Blue Dextran.

Animals↗
PubMedDate not supplied

Removal of heme from preparations of human chorionic somatomammotropin.

The operations involved in the extraction of chorionic somatomammotropin from human placentas cause hemolysis of blood which is present in placental tissue and contamination of crude extract with large amounts of hematic pigments. Most of the pigments are removed by the purification procedure. The portion of purified hCS with high heme content was subjected to treatments with acid acetone and neutral butanone on the basis of procedures generally used for the separation of heme and protein portions in hemoproteins. Both treatments resulted in the removal of heme from hCS; the treatment using butanone was more efficient than the one using acetone. After treatment with both organic solvents, the immunological activity measured with radial immunodiffusion was entirely retained while binding activity on rat ventral prostate particles was slightly decreased.

Acetone↗
PubMedDate not supplied

Purification of human prostatic acid phosphatase and preparation of a specific antiserum in the rabbit.

A modification of previously described methods for the purification of human prostatic acid phosphatase from prostatic tissue is proposed. The purification scheme comprises an Ammonium sulphate fractionation and affinity chromatography on Concanavalin A-Sepharose after dialysis against water. After DEAE-Sephadex A 50 chromatography, the final step is an Ultrogel AcA 34 fractionation. The purified PAP has a specific enzymatic activity of 675 U/mg protein and the total yield is of 32%.

Acid Phosphatase↗