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Biomedical subjects

P Neri

Publications and source records attributed to P Neri.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

Studies on the stability of rat testis membrane receptors for hLH, hCG and hFSH.

Receptor preparations from rat testes were stored at 4 C with various protease inhibitors and chemicals, at -20 C and -80 C, in freeze-dried form as well as at 4 C and 37 C after reconstitution in various media. Each preparation was tested for stability in terms of binding capacity of human luteinizing hormone (hLH), human chorionic gonadotropin (hCG) and human follicle-stimulating hormone (hFSH). The freeze-dried samples were stable for at least six months, although once reconstituted and stored at 4 C, they were less stable than fresh ones. Furthermore, Na azide and Na azide with diisopropylfluoro-phosphate (DIFP) prolonged the stability of the fresh samples. The samples stored at -20 C or -80 C were stable for at least 90 days and repeated freezing and thawing (15 times) did not change the binding capacity of either the hCG/hLH or hFSH receptors.

Animals↗
PubMedDate not supplied

Ultrastructural modifications of the testes and accessory sex glands of genetically dwarf mice after treatment with human chorionic somatomammotropin.

We have studied the effects of treatment with human chorionic somatommatropin (hCS) in the dwarf mouse on the weight and ultrastructure of the testes and accessory sex glands. We used animals of the Snell-Bagg (dw/dw) strain, whose hypophyses secrete neither prolactin nor growth hormone. The effects found were compared with those produced by a similar treatment with ovine prolactin. The administration of hCS causes a significant increase in the wet weight of the testes and accessory sex glands. Ultrastructural observations have demonstrated that hCS is able to stimulate sperminogenesis and the secretory function of the accessory sex glands. Prolactin, as shown in previous reports in the literature, has a similar action on the male reproductive system. However, contrary to the observation after prolactin therapy, no matings were observed after treatment with hCS.

Animals↗