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Biomedical subjects

P Mayer

Publications and source records attributed to P Mayer.

At least 163 records · Page 9Linked to original sources
PubMed1987

Recombinant human GM-CSF induces leukocytosis and activates peripheral blood polymorphonuclear neutrophils in nonhuman primates.

The in vivo efficacy of glycosylated and nonglycosylated recombinant human granulocyte macrophage colony-stimulating factor (rh GM-CSF) expressed in Chinese hamster ovary cells and Escherichia coli respectively was studied in rhesus monkeys following a daily subcutaneous (SC; three times) or intravenous (IV; over six hours) dose for seven consecutive days. The monkeys responded to the rh GM-CSF with a prompt (within 24 hours) rise in circulating white blood cells (WBCs). Thereafter the total cell counts increased steadily in a dose-dependent manner with repeated dosing to numbers six times over the pretreatment levels. Overall, granulocyte counts increased fivefold, lymphocytes twofold to fourfold, and monocytes threefold to fourfold. Platelets and erythrocytes were unaffected. Within 1 week after the end of treatment the leukocytosis had disappeared. Of the two routes of treatment, SC (three times daily)-administered rh GM-CSF was more effective than the same dose given by a six-hour IV infusion. In addition to inducing leukocytosis, parenterally administered rh GM-CSF primed mature circulating granulocytes for enhanced oxidative metabolism and killing of an E coli strain. These results show that exogenously administered glycosylated or nonglycosylated rh GM-CSF is both an effective stimulator of leukocytosis and a potent activator of the phagocytic function of mature granulocytes in monkeys.

Animals↗
PubMed1984

[Prevention of complications following abdominal surgical urologic interventions by plasma protein substitutes].

A prospective randomised trial in 94 patients undergoing urological abdominal surgery has been carried out to evaluate the effect of postoperatively administered plasma proteins. A significant difference between the treatment group and the control group has been found in the incidence of bronchopulmonary complications. This may be due to the substitution of Alpha-1 antitrypsin as could be shown by determination of the activity of Alpha-1 antitrypsin levels. A positive trend but no significant differences could be demonstrated for wound healing and the need of antibiotics postoperatively.

Abdomen↗
PubMed1984

[Therapy of acute radiation cystitis. A case report].

We report a patient with prostate cancer who suffered from severe radiation cystitis after combined interstitial radiation with 125-Iodine-Seeds and external radiation (2000 rad). This patient was treated very successfully with intramural injection of Orgotein (Peroxinorm) into the bladder wall. Besides discussion of the aetiology of radiation cystitis we report our first favourable results with local and systemic treatment with Orgotein (Peroxinorm).

Acute Disease↗
PubMed1984

[Complications following external radiotherapy of prostate cancer. Case reports and overview of the literature].

Complications after definitive radiotherapy of prostatic cancer occur in 3 to 30% of cases. Most common is radiation injury of the urethra, the bladder or the rectum. Definitive radiation therapy of carcinoma of the prostata after staging lymphadenectomy has a considerable risk of lymphedema of the lower extremities. Two typical cases are used to illustrate the permanent damage done to the quality of life of the particular patient. The complication rate is decreased if only the prostate is irradiated without radiation of the regional lymphnodes. Interstitial radiation therapy using 125-Iodine-seeds yields comparable results with a tenfold decrease in the complication rate.

Aged↗
PubMed1984

Stimulation of phagocytosis in bone marrow-derived mouse macrophages by bacterial lipopolysaccharide: correlation with biochemical and functional parameters.

It has been shown that low concentrations of E. coli lipopolysaccharides (LPS) greatly and selectively stimulate phagocytosis and related functions in mouse bone marrow-derived macrophages. Culture in the presence of 50 ng/ml LPS induced on average a 10-fold enhancement of phagocytosis of IgG-coated sheep erythrocytes. Activation was in two stages--a small increase observed during the first 8 to 12 hr, and the major increase noted between 16 and 24 hr. Phagocytic activity remained at the maximal level for 24 hr and then declined progressively. Stimulation by LPS was dose-dependent; significant effects could be observed at 0.8 ng/ml and the maximum was reached at 10 ng/ml. LPS-treated cells also showed a markedly increased tendency to form colonies. All these effects could be prevented by the addition of 100 ng/ml polymyxin B together with LPS, indicating that the active principle is lipid A. The LPS-dependent increase in phagocytic activity is probably mediated by increased Fc receptor capacity because both parameters were influenced in parallel by the stimulus. Phagocytosis-related events, such as enhanced hexose monophosphate shunt activity, H2O2 formation, and nitroblue tetrazolium reduction were also stimulated by LPS. By contrast, pinocytosis was unaffected. Measurements of cell-associated enzyme activities showed that lactate dehydrogenase, acid phosphatase, and cathepsin D were significantly increased. Beta-glucuronidase, beta-galactosidase, alkaline phosphodiesterase, and aminopeptidase were unchanged and NAD nucleosidase was markedly decreased after LPS treatment. 5'-Nucleotidase and glucosamine uptake were undetectable both in control and LPS-stimulated cells. LPS treatment induced a significant increase in cell-associated protein, but did not result in cell proliferation or increased cell loss as shown by the DNA content that remained constant. LPS-induced changes were dependent on de novo protein synthesis; cycloheximide prevented enhancement of phagocytosis, Fc receptor capacity, and colony formation.

Animals↗
PubMed1983

The growth of swine bone marrow cells in the presence of heterologous colony stimulating factor: characterization of the developing cell population.

Conditioned medium prepared from the mouse fibroblast cell line L 929 which is known to produce colony-stimulating factor active on mouse bone marrow cells was also able to stimulate the growth of swine bone marrow cells in a liquid culture system. During the first 4 days of culture mononuclear phagocyte and granulocyte colonies developed. Prolonged cultured cells were classified belonging to the macrophage lineage by morphological and cytochemical criteria. These cells fulfill also functional characteristics for macrophages, like expression of Fc receptors, immune phagocytosis and production of prostaglandins. These bone marrow-derived macrophages could also be activated with LPS and lymphocyte-derived mediators.

Animals↗
PubMed1983

[Experience with the Kaufman prosthesis in the treatment of postoperative urinary incontinence in the male].

22 patients had urinary incontinence plastic surgery according to Kaufman. Good and satisfactory results (n = 14) are to be expected only in cases of genuine stress incontinence following iatrogenic lesion of the sphincteral mechanism. Preoperative urodynamic clarification is obligatory for excluding neurogenic micturition disorders, detrusor-sphincter dyssynergia and bladder instability. Because of the clearly increased complication rate in prostatic cancer, Kaufman-incontinence-plastic surgery should only be carried out when over a period of 12 months tumour progression is not to be expected or when radical prostatectomy was done. Complications with necessary prosthesis removal (n = 9) occurred without exception within 8 weeks after surgery. Among them were two cases of decubital necrosis of the urethra, five cases of secondary wound healing and one case of detrusor-sphincter dyssynergia. General contraindications are: chronically recurrent urinary tract infection, urethral stricture, urethral diverticulum, vesico-urethral reflux with dilated upper urinary tract and the so-called "scarred-rigid urethra".

Aged↗