Search PubMed⌕ Search

Biomedical subjects

P Krieglsteiner

Publications and source records attributed to P Krieglsteiner.

28 records · Page 2Linked to original sources
PubMed1976

Prenatal prediction of respiratory distress syndrome. Measurement of surface properties and Lecithin/Sphingomyelin ratio in human amniotic fluid.

118 samples of amniotic fluid were taken from 102 patients in the 25th to the 43th week of pregnancy by amniotomy or by transabdominal amniocentesis. 76 samples were obtained from patients with normal pregnancies without evidence for either induction or retardation of lung maturity; they served to establish normal values. In 37 cases samples were taken within 72 hours prior to delivery, the decisive period for the prediction of a respiratory distress syndrome. In seven cases a respiratory distress syndrome occured which was diagnosed by an independent pediatrician. The following tests were performed for all samples: 1. Surface tension was measured biomechanically using the Wilhelmy balance and a surface tension area diagram (Fig. 1) was made: A platimum plate is immersed into the liquid placed in the trough; the surface tension is measured via a transducer. The surface film of th liquid to be studied is compressed cyclically from an initial area of 100% to 20% by a moveable barrier and is expanded afterward. Platinum plate and barrier are connected to an X-Y recorder. The surface tension area diagrams are interpreted on the basis of the following criteria: see article 2. The determination of the L/S ratio (simultaneous comparison with the standard method): The method of GLUCK as modified by BORER(planimetric interpretation of the the spots) was employed. Both methods produced normal values. For the biomechanical measurements a classification into three groups was made (25th-30th, 31st-35th, 36th-43rd week of pregnancy) (Tab.I);the L/S ratio showed the usual steep increase from the 36th week of pregnancy onwards. In cases with RDS (Tab.II) surface tension-area-diagrams show high values for gamma max and a slight decrease in surface tension on compression (high gamma min values); the stability index is low, the hysteresis is reduced. Fig. 3. Up to the 34th week of pregnancy the 2:1L/S ratio assumed as the threshold for a sufficient surfactant concentration does not permit a clear interpretation; in subsequent weeks all cases fail to coincide with the normal pattern of distribution (Fig.2). Both the L/S ratio and the criteria of the surface tension area diagrams show significant differences in cases with and without RDS. The prognostic validity of both methods was tested for the probability of predicting occurrence of RDS, probability of RDS occurence and probability of non-occurrence of FDS. The prognostic value seems equal to the L/S Ratio.

Amniotic Fluid↗
PubMed1976

[Biomechanic examination of prenatal lung maturity. Testing of amniotic fluid specimens and lung homogenates on Wilhelmy's scale].

In all traditional methods of determining fetal lung maturity the content of phospholipids in the amniotic fluid is used as parameter. The function of the other parts of the surfactant is not taken into account. The dynamic surface tension measurements using the Wilhelmy balance simulate the interplay of the whole biological system. We have tested 76 specimens of amniotic fluid and 17 lung extracts from 25 weeks' gestation onwards using the biomechanical procedure. In addition, the L/S ratio in the amniotic fluid was determined. There was a correspondence between the values of the amniotic fluid and the lung measured in the same week of pregnancy. The results of the surface tension measurements show a good correlation with the standard method. The following are the advantages of the Wilhelmy balance: results show a small range of dispersion, procedure can be easily learned and carried out quickly and simply.

Amniotic Fluid↗
PubMed1975

[Opportunities for the application of the prostaglandins in gynecology. Interruption of pregnancy in acute leukosis. Description of a case].

Prostaglandin is being used increasingly as an abortifacient, especially in infans mortuus, fetus mortuus, interruption of pregnancy, missed abortion and hydatidiform mole. The most commonly used form of application is extraamniotic instillation. Interruption of pregnancy during acute leukosis of pregnancy is an extension of the list of indications. One such case is reported. The reliable and rapid onset of action and the small loss of blood suggest that prostaglandins may be the drug of choice.

Abortifacient Agents↗
PubMedDate not supplied

[Pharmacological influences on fetal lung maturity, part V: Influencing the pulmonary DPL (dipalmitoyl lecithin) level with L-thyroxin (T4)].

Dipalmitoyl lecithin (DPL = DPPC, dipalmitoyl phosphatidyl choline) is both quantitatively and functionally the most important constituent of the surfactant complex. The effect of thyroxin (T4) on fetal lung maturity was investigated by means of selective DPL determination. Following administration of thyroxin to the mother animal an increase in the DPPC content was demonstrated in rat fetuses. This increase was greater than that of the total phospholipids. This effect of thyroxin could be due to the following causes: 1, Earlier and accelerated differentiation of lung cells: more Type II cells are formed earlier and these synthesize DPPC more effectively than other lung cells (approx. 40-50% of the phosphocoline in Type II cells in DPPC). 2. Stimulation of the deacylation-reacylation cycle. 3. A combination of these mechanisms.

Amniotic Fluid↗