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Biomedical subjects

P Jean

Publications and source records attributed to P Jean.

At least 55 records · Page 3Linked to original sources

Light and electron microscopy of Ag-NORs in domestic horse chromosomes identified after R-banding.

Silver staining shows the presence in the domestic horse of six NORs located on chromosomes 1, 26 and 31 as identified after R-banding. Following electron microscopy, the argyrophilic material was observed outside the terminal secondary constrictions (satellite stalks) on the terminal portion of the short arm of chromosome 1, outside the secondary constrictions on the proximal region of the long arms of chromosome 31, and beside the proximal region of the long arms of chromosome 26. Satellite staining applied to these chromosomes appears to reveal only the active NORs.

Animals↗

Daily evaluation of circulating granulocyte-monocyte progenitors during bone marrow recovery from induction therapy in acute leukemia.

The notable increase of the circulating granulocyte-monocyte progenitors (PB CFU-GM) during bone marrow recovery following chemotherapy is a well known phenomenon. It has led to consider harvesting a large number of autologous stem cells by cytapheresis. Daily assessments have been conducted on the PB CFU-GM level in 9 patients with acute leukemia at the time of bone marrow regeneration after the first induction course in order to identify the circumstances of this rise. The PB CFU-GM maximum peak, of an average of 2142/ml, occurs between day 17 and day 23 after the chemotherapy has ended. A ten-fold increase of the PB CFU-GM level above normal values is maintained between 2 and 10 days. The PB CFU-GM peak coincides with that of the circulating immature myeloid cells and monocytes. The platelet rise above 100 X 10(9)/1 always occurs 2-7 days before the PB CFU-GM peak.

Acute Disease↗

[Peripheral neuropathy during treatment with cimetidine].

A case of peripheral neuropathy following cimetidine treatment is reported. Four days after beginning cimetidine (200 mg four times a day), the patient developed muscle pain and a symmetric motor neuropathy in all 4 limbs, predominant distally and in the lower limbs. Cimetidine was discontinued. Within seven days motor function began to return and within five months recovery was complete. Electrophysiological studies showed an axonal neuropathy. Morphometric studies revealed loss of large myelinated fibers in some fascicles while other fascicles were normal. Teasing studies showed predominant axonal lesions. Microvasculitis was present in the epineurium. Such findings suggest a role for small-vessel immune-complex vasculitis in the pathogenesis of this cimetidine-induced peripheral neuropathy.

Adult↗

[Acute epididymitis].

A series of 86 cases of acute epididymitis observed over a period of 3 years is analyzed and findings reported. Testicular lesions were frequent and 35% of these cases involved a purulent testicular pocket. The epididymal lesion was usually of a total nature (77% of cases), only the tail of the epididymis being involved in 17%. Sterile urine was a frequent finding (50% of cases). Chlamydiae were rarely identified, but administration of medication ineffective against chlamydiae was effective in most cases, suggesting that these organisms are a rare cause of acute epididymitis. Iatrogenic epididymitis was a frequent diagnosis when compared with epididymitis due to cases of known cervico-urethral obstruction. Catheterization, endoscopic resection of prostate, transvesical prostatic adenomectomy were the main cause in the absence of ligature of deferens, the latter being considered as a certain preventive procedure. The affection is possible in children or adolescents (3 cases between 10 and 16 years) with an acute hydrocele as the presenting sign. Tuberculosis is still a possible cause (5 cases) with Koch's bacillus present in the urine in 4 of these patients. In the majority of cases, however, the etiology was unknown (62 of 86 patients = 72%). Treatment can be by a cycline as sole therapy, anti-inflammatory steroid or non-steroid agents also being effective. Medical treatment was rapidly and totally effective in 56 of the 86 patients, but residual foci were noted in 19 of 86 cases. Epididymectomy may be necessary for painful lesions or recurrence of epididymitis, while castration may be indicated (6 cases) for fistula formation, purulent pockets of testis, or ischemic necrosis of testis.(ABSTRACT TRUNCATED AT 250 WORDS)

Acute Disease↗

[Parapyelic cysts].

Parapyelic cysts present particular characteristics in relation to cystic disease of the kidney. Their intrasinusal development in contact with vascular and canalar elements of the renal hilum explains their often explosive symptomatology and the frequent need for surgical excision of the cyst--procedure difficult to accomplish and nearly always incomplete. Four cases are reported, including 2 with cysts communicating with excretory pathways, and symptoms, signs and diagnostic and therapeutic aspects discussed. Arguments are in favor of an acquired pathogenicity of these cysts (communication with excretory pathways, contingent presence of smooth muscle fibres in the cyst wall).

Colic↗

[Cystic form of kidney cancer. Apropos of a case].

The authors report a case of cystic form of carcinoma of the kidney in a patient who also had a bladder tumour which could have explained the presenting symptom of haematuria. It was the discordance between echotomography and CT scan findings which led the authors to explore the patient surgically. A reassuring result of a frozen section biopsy led to partial nephrectomy even though the cystic mass had thick walls and serosanguinous contents. The actual diagnosis was corrected within 24 hours by the histopathologists who reported a clear cell renal carcinoma. The authors performed an extended nephrectomy which revealed the presence of some malignant cells in the remaining renal fragment. They take this opportunity to review the general problem of the association of carcinoma of the kidney and renal cyst. This case shows how hazardous may be the answer given from frozen section biopsy of a cystic mass in the kidney and how dangerous it is to perform a partial nephrectomy which may leave tumour tissue in place.

Carcinoma, Renal Cell↗

Association of centromeric heterochromatin with the nucleolus in mouse Sertoli cells.

In adult Sertoli cells of most strains of mice, all the centromeric heterochromatin is condensed in two chromocenters, one on each side of a single, large nucleolus. In a random-bred Swiss OF-1 strain, however, the nucleus has a different structural organization. Part of the heterochromatin Is seen as chromocenters in contact with the nucleolus; the rest of it is dispersed in granules of unequal size in the nucleoplasm. Such an unusual spatial arrangement of heterochromatin in interphase nucleus cannot be explained either by a difference in the nucleolar organizing regions or by a polymorphism of the C-banding of metaphase chromosomes.

Animals↗

High-resolution idiogram of Giemsa R-banded human prophase chromosomes.

The schematic representation of RHG-banded chromosomes (R-banding was produced by heat denaturation followed by Giemsa staining (RHG) in the 850-band range per haploid set, was prepared showing the relative position, the specific size, and the characteristic staining intensity for each band. To this idiogram was adapted the new International Standard Cytogenetic Nomenclature. Our aim was to produce a realistic idiogram which could help in the preparation of R-banded prophase karyotypes and in the localization of chromosomal rearrangements. A comparative analysis of bands at prophase and metaphase revealed certain aspects of the dynamics involved in chromosome condensation and in R-band organization. The effect of chromosome elongation on the appearance of R-bands within heterochromatic regions has also been discussed.

Adult↗

[Toxicity and dangers of volatile nitrites sold in sex shops].

The authors report a case of methemoglobinemia following a suicidal attempts with a popper containing isobutyl nitrite. The toxic effects of this substances, either by ingestion or inhalation, are described as well as risks of use in "everyday life". Regulations, still incomplete has been suggested in USA. In France, preventive measures should be taken.

Adult↗

Fragile X chromosome and X-linked mental retardation.

A family is described in which three normal females transmitted to seven males X-linked mental retardation associated with macro-orchidism and a fragile site on the long arm of the X chromosome -- fra(X)(q27). The affected males also had minor clinical features in common: a large forehead, long face, large ears, a long upper lip and large extremities.

Adolescent↗