Cutaneous protothecosis in a cat.
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Animals↗
Biomedical subjects
P J Coloe
Publications and source records attributed to P J Coloe.
Ethanol formed from arabinose: a rapid method for detecting Escherichia coli.
Head-space gas-liquid chromatography showed that Escherichia col are consistent in their ability to ferment arabinose with the production of ethanol. In both still and shaken cultures ethanol production is closely aligned with growth. Also ethanol production is related to inoculum size so that varying sizes of inocula may be distinguished by choosing a time of sampling early in growth. The method is suitable as a rapid test for E. coli and is applicable for use in detecting E. coli urinary tract infections.
Air↗
Head-space gas liquid chromatography for rapid detection of Escherichia coli and Proteus mirabilis in urine.
Measuring ethanol formed from arabinose by Escherichia coli and dimethyl disulphide formed from methionine by Proteus mirabilis is a rapid way of detecting these organisms in the urine. Ethanol and dimethyl disulphide are detected by head-space gas-liquid chromatography. Since sedimented organisms and not whole urine are used the method is not subject to interference from compounds within the urine. When it was tested on 122 samples of urine, 94 from patients with suspected urinary infection and 28 supposedly uninfected control specimens, the results correlated well with viable counts by a conventional bacteriological method. As well as being rapid (results available in four hours) the method is reliable, easy to use, and could be automated. It now requires extensive testing in a hospital laboratory.
Bacteriological Techniques↗
The importance of prolonged incubation for the synthesis of dimethylnitrosamine by enterobacteria.
Tests with 140 strains representing Escherichia coli, Klebsiella aerogenes, K. ozaenae, Proteus mirabilis, P. vulgaris, P. rettgeri and P. morganii in a defined medium supplemented with 0-09M dimethylamine (DMA) and 0-1M potassium nitrate showed that at least 89% of the 136 strains able to reduce nitrates produced up to 9mM dimethylnitrosamine (DMN) in 70h at 37 degrees C. Four nitratase-negative strains produced DMN from DMA in the presence of sodium nitrate. Prolonged incubation was the most important factor in determining DMN production. Stasis and persistent infection in the urinary tract, by simulating prolonged incubation of a culture, may be of importance in determining whether the potential carcinogen, DMN, could be produced in vivo by bacterial action on DMA and nitrate in urine.
Dimethylamines↗
Humoral responses and salmonellosis protection in chickens given a vitamin-dependent Salmonella typhimurium mutant.
Humoral responses in chickens inoculated with an aromatic vitamin dependent (Aro-) Salmonella typhimurium mutant (STM) were studied to ascertain the efficacy of the organism as a vaccine for salmonellosis and possibly as a delivery system for antigens from enteric pathogens of chickens. Serum antibody responses in chickens that were given oral or subcutaneous inoculations of the bacterium followed the classic order of antibody production, with IgM being detected first, followed by IgG and IgA. Antibody responses in the gut of orally inoculated chickens were restricted to IgG and IgA. Weight gain measured in chickens given high doses of STM (up to 5 x 10(9)) orally, revealed that the bacterium did not adversely affect the chickens; in fact, inoculated chickens had significantly higher body weights than controls at the same age. Salmonellosis protection of chickens by oral vaccination with STM was examined in a vaccination/challenge experiment. The experiment revealed that oral vaccination reduced excretion of a virulent S. typhimurium used as the challenge organism.
Animals↗