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Biomedical subjects

P Huber

Publications and source records attributed to P Huber.

At least 163 records · Page 9Linked to original sources
PubMed1986

[Acute space-occupying processes in the posterior cranial fossa. Clinical aspects and computerized tomographic findings].

Ascending transtentorial herniation and compression of the brain stem are very severe sequelae of acute space-occupying lesions of the posterior fossa such as hematoma, infarction and tumours. The symptoms, course and outcome of these acute space - occupying lesions are discussed on the basis of 50 patients. For correct diagnosis of transtentorial herniation, CT studies are essential. The signs can be divided into early and alarming, and the time course into a biphasic and foudroyant form. Early detection of transtentorial herniation or impending incarceration is of crucial importance for successful therapy.

Acute Disease↗
PubMed1986

Cell-free synthesis, membrane integration, and glycosylation of pro-sucrase-isomaltase.

Cell-free translation of total RNA from rabbit intestinal mucosa in a rabbit reticulocyte lysate, after immunoprecipitation with antibodies directed against sucrase-isomaltase, yielded a polypeptide of 200 kDa, which was identified as pro-sucrase-isomaltase. Addition of dog pancreatic microsomal vesicles to the translation system resulted in the appearance of an additional 220-kDa polypeptide. The 220-kDa polypeptide was associated with the membranes in a way that made it inaccessible to proteolysis; this protection was abolished by lytic detergent concentrations, indicating that the polypeptide was segregated into the microsomal vesicle. The 220-kDa polypeptide was glycosylated as evidenced by it being bound to concanavalin A-Sepharose and eluted with alpha-methyl-D-mannopyranoside. The increase in apparent molecular mass (approximately 20 kDa) of the primary translation product upon translocation was due to the addition of carbohydrate; treatment of the 220-kDa polypeptide with endo-beta-N-acetylglucosaminidase H increased its electrophoretic mobility to that of the 200-kDa polypeptide which was obtained in the absence of membranes. Partial N-terminal amino acid sequence of a translation product labeled with [3H]Leu in the absence of membranes revealed that Leu was incorporated into identical positions as in the final (pro)-sucrase-isomaltase, thus indicating the lack of a transient signal peptide.

Animals↗
PubMed1986

Localization of the precentral gyrus in the computed tomogram and its clinical application.

In 30 patients the anatomical configurations of the precentral and central sulcus and gyrus were examined by computed tomography and could be determined with great accuracy. The distances between the coronal suture and the central and precentral fissure, and the angle between the longitudinal fissure and the central fissure were measured. These data are used in the precise localization for a surgical approach to a mass lesion.

Humans↗
PubMed1986

A transcranial Doppler method in the evaluation of cerebrovascular spasm.

An ultrasonic Doppler method was used to monitor flow velocities in basal cerebral arteries in 21 patients with spontaneous subarachnoid hemorrhage (SAH). The time course of vasospasm as evaluated by this technique was similar to that reported in angiographic studies. In 82% of the patients an increase in intracranial velocities to 120 cm/s or more was found during the second or third week after hemorrhage. (Normal value 62 cm/s). Arterial narrowing giving rise to velocities above 200 cm/s was classified as severe spasm. This occurred in 42% of the cases, and a significant decrease in flow velocity in the extracranial carotid artery was found in this group.

Adult↗
PubMed1986

Spatial and quantitative distribution of human peritumoural brain oedema in computerized tomography.

Computerized tomography (CT) was used to study the pathways of oedema spreading in man. Based on the assumption that local changes in CT numbers in oedematous white matter closely correspond to changes in tissue water content, CT numbers of consecutive tissue blocks of 3.0-3.6 mm were examined in the main directions of oedema spreading: towards the deep white matter, towards the cortex and towards the ventricle. Tumours with oedema grade II and III showed a reduction of CT number of 10 +/- 1.8. The corresponding increase in water content of about 10-12% seems to be an upper limit of fluid accumulation in the white matter. From this oedema centre, water content very slowly and gradually decreased along the oedema projection into the deep white matter. In contrast, if oedema reached the cortex of adjacent gyri, the decline in water content was very sharp. A similar observation was made in the external capsule where oedema sharply declined at the border to the adjacent grey matter, putamen and claustrum. Oedema projection towards the ventricle showed a nearly uniform magnitude from the centre to the ventricular lining, suggesting a certain resistance by a limited capacity of transependymal drainage of oedema fluid. It is assumed that the spatial distribution and extension of oedema around a brain tumour is determined by a system of differential resistance to fluid movement in the following order: grey matter--ventricular lining--white matter.

Brain Edema↗
PubMed1986

Cerebral vasospasm evaluated by transcranial ultrasound correlated with clinical grade and CT-visualized subarachnoid hemorrhage.

In 39 patients with a proven subarachnoid hemorrhage (SAH), the clinical status, the amount of subarachnoid blood on a computerized tomography scan obtained within 5 days after SAH, and the flow velocities (FV's) in both middle cerebral arteries (MCA's) measured by transcranial Doppler sonography were recorded daily and correlated. All patients had pathological FV's over 80 cm/sec between Day 4 and Day 10 after SAH. The side of the ruptured aneurysm showed higher FV's than did the unaffected side in cases of laterally localized aneurysms. Increase in FV preceded clinical manifestation of ischemia. A step early increase of FV's portended severe ischemia and impending infarction. Maximum FV's in the range of 120 to 140 cm/sec were not critical and in no case led to brain infarction. Maximum FV's over 200 cm/sec were associated with a tendency for ischemia, but the patients may remain clinically asymptomatic. In cases of no or only a little blood in the basal cisterns, mean FV's in both MCA's increased only moderately whereas, with thick clots of subarachnoid blood, there was a steeper and higher increase of mean FV's.

Adult↗
PubMed1986

High resolution CT angiography in the direct diagnosis of cerebral aneurysms.

By performing a rapid series of 1.5 mm slices, with high resolution CT angiography (angio-CT), after intravenous bolus injection of 100 ml of contrast medium, an image of the basal arteries similar to that of conventional angiography may be obtained. A consecutive series of 102 patients with suggested cerebral aneurysms were examined by angio-CT and, as a control, were also studied with selective four-vessel angiography and/or at autopsy. In 33 patients angiography or autopsy revealed no aneurysm; 69 patients were shown to have 76 aneurysms. The angio-CT was correctly negative in 30 patients (91%), falsely suggested in 2 (6%), and falsely positive in 1 patient (3%). Sixty-eight aneurysms (89%) of down to 3 mm in size were correctly demonstrated by angio-CT, 6 (8%) were correctly suggested, and 2 aneurysms (3%) escaped CT demonstration.

Cerebral Angiography↗
PubMed1986

Transcranial Doppler sonography. An alternative to angiography in the evaluation of vasospasm after subarachnoid hemorrhage.

With transcranial Doppler sonography (TCD) the blood flow velocity (FV) in the basal cerebral arteries can be measured non-invasively. Vasospasm produces an increase of the FV in the narrowed arterial segment which can be evaluated by TCD. A clear correlation between the diameter of the artery and the increase in FV could be demonstrated in the middle cerebral artery. Vasospasm can be detected by TCD non-invasively and angiography can be avoided during the critical phase. The time course of vasospasm can be followed by TCD instead of repeated angiographies, enabling an optimal timing of aneurysm surgery.

Adult↗
PubMed1986

[Breast reconstruction with the transverse abdominal island flap].

A transverse abdominal island flap (TAIF) harvested from the ipsi or contra-lateral side, has been employed for breast reconstruction in five patients. The use of a silicone implant can be avoided because of the adequate bulk of skin, muscle and fat that is available. Examples of representative cases are shown.

Breast↗
PubMed1985

Serum half life of prostatic acid phosphatase.

Traumatic manipulation of the prostate can cause elevated prostatic acid phosphatase (PAP) values. To avoid falsely elevated PAP values after prostatic trauma we studied the serum half life of PAP and the time taken to return to preoperative levels in patients undergoing transurethral resection (TUR-P). Although we observed a broad variation in peak PAP values the half life of PAP is fairly constant at about 1.1-2.6 h. Preoperative values were reached within 30 h. We conclude that PAP determinations can safely be carried out 30 h after prostatic trauma without any risk of falsely elevated PAP values.

Acid Phosphatase↗
PubMed1985

Effects of ethylbenzene, toluene, and xylene on the induction of micronuclei in bone marrow polychromatic erythrocytes of mice.

Genotoxic effects of five widely used aromatic industrial solvents, ethylbenzene, methylbenzene (toluene), o-, m-, and p-dimethylbenzene (xylene), on bone marrow cells of male NMRI mice were studied using micronucleus test. Each compound was given to animals by IP administration of two similar doses 24 h apart. Increased formation of micronuclei within polychromatic erythrocytes of femoral bone marrow 30 h after the first injection was conducted to be due to the clastogenic effect of the test compound. Of the chemicals tested, only toluene gave a dose-dependent increase in the frequency of micronucleated polychromatic erythrocytes. This genotoxic activity of toluene was confirmed in male B6C3F1 mice.

Animals↗
PubMed1985

Phosphocreatine content and intracellular pH of calf muscle measured by phosphorus NMR spectroscopy in occlusive arterial disease of the legs.

Energy metabolism of calf muscle was assessed non-invasively by phosphorus (31P) NMR spectroscopy in eleven patients with symptomatic arterial occlusion and in seven matched controls. Phosphocreatine (PCr) content and pH values decreased during non-ischaemic foot exercise to lower values in severely afflicted patients but in all patients, as a group, they were not significantly decreased compared to controls. In contrast, recovery from ischaemic exercise (arterial occlusion by a tourniquet) demonstrated significant differences between patients and controls. Intracellular pH and PCr recovered more slowly in patients than in controls; PCr recovery proceeded exponentially with a recovery half-time of 203 +/- 74 s in patients compared to 36.7 +/- 5.5 s in controls (P less than 0.02). Phosphocreatine (PCr) recovery after ischaemic exercise correlated significantly with the degree of arterial stenoses as assessed by Doppler ultrasound (r = 0.739, P = 0.019) and by angiography (r = 0.885, P = 0.005), suggesting that the degree of large vessel stenoses limits the postischaemic increase in mitochondrial oxidative phosphorylation. Reactive blood flow after ischaemia failed to correlate with PCr recovery or with the degree of arterial stenoses. Phosphorus (31P) NMR spectroscopy provides, therefore, quantitative parameters of muscle energy metabolism in patients with peripheral arterial occlusions.

Arterial Occlusive Diseases↗
PubMed1985

Assessment of intracranial hemodynamics in carotid artery disease by transcranial Doppler ultrasound.

Noninvasive transcranial Doppler recordings were correlated to the angiographic findings in 77 patients with carotid artery disease. Stenoses reducing the luminal area of the internal carotid artery by 75% or more also reduced the pulsatility transmission index (PTI) of the ipsilateral middle cerebral artery (MCA). The PTI is the pulsatility index of the artery under study expressed as a percent of the pulsatility index of another intracranial artery with presumed unimpeded inflow in the same individual. For stenoses in the 75% to 89% category. PTI reduction was significantly greater in patients with bilateral carotid stenosis, indicating an impaired potential for collateral flow in these patients. The PTI reduction probably reflects both the pressure drop across the stenosis and the cerebral autoregulatory response. Two criteria proved useful in demonstrating collateral MCA supply through the circle of Willis. On the recipient side, retrograde flow in the proximal anterior cerebral artery was demonstrated in 29 of the 31 patients when this flow pattern was disclosed angiographically. In 26 of these patients, the anterior cerebral artery on the supplying side also had clearly increased flow velocity. Increased flow velocities in the proximal posterior cerebral artery were present in 26 of the 30 vessels that were acting as a collateral channel to the ipsilateral MCA.

Adult↗
PubMed1985

57Co-labelled bleomycin scintigraphy for the detection of lung cancer: a prospective study.

The sensitivity and specificity of scanning with 57Co-labelled bleomycin for the diagnosis of lung cancer were determined from a prospective study involving a large group of patients with an abnormal chest roentgenogram. A semi-quantitative analysis of the scintigram was obtained by defining an index in terms of the excess isotope uptake within the tumour. Malignant pulmonary lesions were found in 121/175 patients and 113 had a positive scan. Benign pulmonary disorders were found in 54 patients, 45 of whom had a negative scan. The sensitivity and specificity were therefore 93% and 83% respectively. Bayes' theorem was then used to determine the predictive value of this test, either positive or negative, when the prevalence of disease varied from 10 to 90%. A receiver operating characteristic curve permits the choice of an appropriate value of the tumour uptake index to minimize the false positive fraction without classifying too many true positives as false negatives.

Adenocarcinoma↗
PubMed1984

[Indications and limitations of computer tomography studies in headaches and seizures].

Examination of the records of patients with headache or with generalized epileptic seizures, but without neurological disorders or any sign of intracranial hypertension, shows that in this group of patients expansive processes are rare. If in this group of patients a tumor is nevertheless discovered by CT (as it is in 3.3% of patients with headache and in 8% of those with generalized epileptic seizures) it becomes evident that with very few exceptions the neurological status and/or EEG of these patients was not absolutely normal either. By careful evaluation of the patient's history and the neurological status - supplemented in patients with epileptic seizures by an EEG - many unnecessary computed tomographies could be avoided.

Adolescent↗
PubMed1984

[Application of computerized tomoscintigraphy in the detection of tumors using cobalt-57-marked bleomycin].

57Co-bleomycin scanning dates from the early 1970s, when the first results were presented by Renault et al. Since then there have been numerous publications concerning the high sensitivity of this type of study in the detection of pulmonary or cerebral neoplasms. In the region below the diaphragm, however, sensitivity was poor due to hyperactivity of the kidney, liver and bladder. To eliminate this problem the authors have used single photon emission computerized tomography (SPECT), and their department is currently investigating the sensitivity of this technique for the detection of neoplasms. Two typical cases are described which illustrate the value of this method in the detection of cancer.

Abdominal Neoplasms↗
PubMed1984

Elevation of serum prostatic acid phosphatase levels after prostatic massage.

The effect of prostatic massage on the serum prostatic acid phosphatase (PAP) levels determined by radioimmunoassay (RIA) was studied in 29 patients with benign prostatic hyperplasia (BPH) and 7 patients with prostatic carcinoma (CA). Among the BPH patients, 77 per cent (P less than 0.001) showed an increase in post-massage PAP levels but only 3 (10%) showed an increase to more normal levels.

Acid Phosphatase↗