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Biomedical subjects

P Heczko

Publications and source records attributed to P Heczko.

At least 37 records · Page 2Linked to original sources

[Occurrence of trichomoniasis in women with intra-uterine devices (author's transl)].

The authors investigated the biocenosis in the vaginas of 216 women with IUD and 124 controls without any contraceptive. The incidence of vaginal trichomoniasis in IUD probands was higher with significance than that among the controls. Culture testing is indispensable to rule out the presence of vaginal trichomoniasis. Parasites were not readily detectable from stained and native specimens unless infections were massive. The cytological findings recorded from the above probands were within the normal. Knowledge is inadequate on the risk of infection of women with IUD. This should be another reason for which to improve public health education.

Adult↗

Serological characterization of phages of coagulase-negative staphylococci.

Selected 22 phages of coagulase-negative staphylococci were tested against group-specific antisera for Staphylococcus aureus phages using a neutralization test. Fourteen of the tested phages were classified into the serological group B and one into the group F. All other phages were resistant to the antisera against S. aureus phages but cross-reacted with the antisera produced against themselves. Therefore they could be joined together into a new serological group of the staphylococcal phages.

Bacteriophage Typing↗

Detection and preliminary characterization of extrachromosomal DNA in clinical isolates of Staphylococcus aureus and Staphylococcus epidermidis.

Isolates of Staphylococcus aureus and Staphylococcus epidermidis frequently harboured extrachromosomal DNA. From the 20 Staphylococcus aureus strains examined 85% contained extrachromosomal DNA and 65% carried more than one plasmid DNA species. The results obtained with the 19 Staphylococcus epidermidis strains analyzed were similar, 84% of the strains had extrachromosomal DNA and 68% contained several DNA species. The agarose gel electrophoresis of the DNA obtained from cleared lysates of the staphylococci proved to be efficient for the detection of plasmids and the determination of their molecular weights. The gel method appeared therefore to be suitable for the establishment of the role of plasmids in the phage typability of staphylococci. Using a Staphylococcus epidermidis strain as an example it was shown by electron microscopy that the extrachromosomal DNA demonstrated by the agarose gel electrophoresis represented covalently closed circular plasmid DNA. This plasmid DNA could be sufficiently labeled with 3H-thymidine to undertake studies on the relationship and molecular characterization of staphylococcal plasmids.

DNA, Bacterial↗