Search PubMed⌕ Search

Biomedical subjects

P Fuchs

Publications and source records attributed to P Fuchs.

At least 91 records · Page 5Linked to original sources
PubMed1982

Interaction of phencyclidine with mouse neuroblastoma cells.

Growth of mouse neuroblastoma (Nb) cell (clone M1) was not affected by phencyclidine (PCP) concentrations of 10(-6)M up to 2 x 10(-4)M, whereas 10(-3)M PCP caused a 100% inhibition of cell growth. Several PCP analogs, including the quaternary PCP methiodide, exerted effects similar to those of PCP. The uptake of [piperidyl-3,4-3H]PCP ([3H]PCP) by the Nb cells was studied using cell monolayers in Petri dishes. Non-specific entry of PCP into the cells was linear with added substrate but specific uptake exhibited saturation kinetics. The concentration for half-maximum specific uptake was 2 x 10-(5)M, and the capacity of the cells at saturation was 2-3 nmoles [3H]PCP/mg protein, at 22 degrees. The uptake rate constant was 0.2 +/- 0.05 x 10(5) (M-1 min-1) and the dissociation constant was 0.25 +/- 0.05 (min-1). Uptake was temperature dependent and was inhibited by 2,4-dinitrophenol (DNP). This may indicate that this binding represents (at least in part) an active uptake process of PCP into the cells.

Animals↗
PubMed1982

[Selection of antibiotics and practice and the clinic].

The multiplicity of antibiotics currently available for the treatment of bacterial infections in outpatients and inpatients has led to considerable confusion and uncertainty in recent years. An attempt is made to establish indications for the use of the various groups of antibiotics, paying due regard to cost as well as other aspects. This means drawing a distinction between the respective needs of out- and inpatients. In treating outpatients, where resistance is less of a problem than in hospitals, use is made principally of oral substances. In the light of current knowledge the vast majority of bacterial infections occurring in ambulatory practice can be optimally treated with pyrimidine-sulfonamide combinations, penicillins, tetracyclines and erythromycin. In contrast, the range of broad-spectrum antibiotics administered in hospitals, for the most part parenterally, in infections of a life-threatening nature or due to organisms exhibiting multiple resistance, is quite different. Most important are the more recent penicillins and cephalosporins and the aminoglycosides. By combining betalactam antibiotics with aminoglycosides and attempt is made to extend the therapeutic spectrum and intensify antibacterial activity. A conclusive judgement on the therapeutic differences between the more recent penicillins and cephalosporins as single-substance therapy or in combination with aminoglycosides is not yet possible at present. There seems good reason, however, to combine the more recent penicillins with aminoglycosides in infections in which enterococci, pseudomonas and anaerobic bacteria are also involved. In infections in which there is a possibility of Klebsiella involvement or in which penicillin allergy or penicillin resistance is present, treatment tends to be a combination of cephalosporins and aminoglycosides.

Aminoglycosides↗
PubMed1980

Nature of permeability changes in membrane of HeLa cells adsorbing Sendai virus.

Adsorption of Sendai virus to HeLa cells induced in them an increased permeability to K+, Na+, Ca++, deoxyglucose, but not to fluorescein. The stimulation of uptake of 42K was temperature-dependent, did not occur below 15 degrees C, and was not inhibited by ouabain. The virus-induced increase in the uptake and release of 42K and of 3H deoxyglucose could not be mimicked by treatment of cells with linoleic acid, a procedure which increased the fluidity of the cellular membranes. The stimulatory effect of 0.5 mM ATP on the release of deoxyglucose was enhanced several fold in the presence of Sendai virus. These results seem to indicate the possible involvement of membranal enzymes such as e.g. protein kinase in the permeability changes induced by Sendai virus.

Calcium↗
PubMed1979

[Routine diagnosis of bacteriuria: estimation of urinary sediment or counting blood cells? (author's transl)].

Testing of a method of bacteriuria diagnosis which would be feasible for outpatient routine work and highly reliable while requiring little cost and effort, revealed the clear superiority of examining the uncentrifuged urine in the counting chamber over the sedimentation method. Whereas the accuracy of the sedimentation method in bacteriuria diagnosis is about 50%, the counting chamber method gave correct results in more than 90% of the cases. This entails saving in terms of manhours. The only drawback of the counting chamber method is its higher rate of false-positive findings caused by the fact that the microscopic identification of bacteria enters into the final count. This drawback, however, is more than made up by the reduced amount of material and time involved in this procedure.

Bacteriuria↗
PubMed1978

Inhibition of adsorption of West-Nile and herpes simplex viruses by procaine.

The effect of the local anaesthetic drug procaine on the adsorption of two enveloped viruses was studied. Physiological concentrations of the drug (7 X 10(-3)--7 X 10(-2) M) strongly inhibited the adsorption of both West-Nile and herpes viruses as determined by plaque assay and the infective center assay.

Adsorption↗
PubMed1978

[Casting faults and structural studies on bonded alloys comparing centrifugal castings and vacuum pressure castings].

The casting processes in use today such as centrifugal casting and vacuum pressure casting were compared with one another. An effort was made to answer the question whether the occurrence of shrink cavities and the mean diameter of the grain of the alloy is dependent on the method of casting. 80 crowns were made by both processes from the baked alloys Degudent Universal, Degudent N and the trial alloy 4437 of the firm Degusa. Slice sections were examined for macro and micro-porosity and the structural appearance was evaluated by linear analysis. Statistical analysis showed that casting faults and casting structure is independent of the method used and their causes must be found in the conditions of casting and the composition of the alloy.

Crowns↗
PubMed1978

[New antibiotics in the treatment of urinary tract infections].

Based on the frequency of the isolated bacteria the antibiotics and chemotherapeutics commonly used in infections of the urinary tract are discussed. It turns out that ampicillin and sulphamethoxazole-trimethoprim are still the drugs of choice in the treatment of these bacterial diseases. A true advance is achieved by the introduction of carbenicillin and the new aminoglycoside amikacin. Carbenicillin can be used in Pseudomonas infections and is characterized by its low toxicity. Amikacin is the drug of choice against aminoglycoside-resistant gramnegative bacteria.

Amikacin↗