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Biomedical subjects

P Fritz

Publications and source records attributed to P Fritz.

At least 127 records · Page 7Linked to original sources
PubMed1989

Radiological evaluation of temporal bone disease: high-resolution computed tomography versus conventional X-ray diagnosis.

Sixty-two patients with different temporal bone lesions were prospectively examined by high-resolution computed tomography (CT) and conventional plain radiography, including pluridirectional tomography. High-resolution CT enabled a clear diagnosis in 80% of cases, conventional radiology in 63%; 1.6-times more bone information was recorded by high-resolution CT which is clearly superior for imaging cholesteatomas, metastases and inflammatory processes and for evaluating osseous destruction. With regard to pathological soft tissue or effusions filling the tympanic cavities, conventional radiology shows poor sensitivity (0.61). High-resolution CT is the most sensitive method for the imaging and classification of temporal bone fractures, including labyrinthine damage and ossicular chain injuries. Only in cases of atypical fractures with an unfavourable relationship to the CT planes, can carefully directed tomography be more effective. In most cases high-resolution CT replaces conventional radiology and should be the method of choice for comprehensive radiological examination of the temporal bone.

Bone Diseases↗
PubMed1989

Quantitative immunohistochemistry: standardization and possible application in research and surgical pathology.

We describe three different methods of calibration of quantitative immunohistochemistry, two of them based on self-made dye-agarose or antigen-agarose substrates. Quantification of immunostained objects (immunosignals) can be done by (1) microscope photometry (plug method) or scanning-photometry; (2) and densitometry of a television transformed microscope image. Both approaches are subjected to the different methods of calibration and three examples of application.

Arginase↗
PubMed1989

Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

HeLa Cells↗
PubMed1989

[Results of local radiotherapy and modified total-brain irradiation in astrocytoma].

A retrospective evaluation was made on the results of primary or postoperative radiotherapy in 162 patients with grade I to IV astrocytomas. The greatest part of the overall group was built by 113 patients suffering from glioblastoma multiforme. For the survival analysis, the prognostic importance of the following factors was investigated: sex, age, surgery, radicality of surgery, grading according to Kernohan, tumorous lesions in central structures, and target volume of radiotherapy. The prognosis is first of all dependent on the operability, the grading, and the age of the patient. The survival of patients with malignant gliomas is not influenced by the radicality of the operation and the extension of the target volume to the whole brain (total-brain irradiation and boost). Thus in the treatment of malignant astrocytomas, the less extensive surgical and radiotherapeutical intervention seems an adequate method to manage this disease.

Adolescent↗
PubMed1989

Rectus femoris sheath: a new localization of hip synovial cyst.

Iliopsoas bursitis is the classical manifestation of synovial cysts of the hip. We report the first observation of a new localization of these cysts: the sheath of rectus femoris muscle. The patient presented with a hip osteoarthritis and an important mass of the front of the thigh. Computed tomography was useful in establishing the diagnosis and hip arthrography confirmed the communication between the joint and the rectus femoris sheath.

Arthrography↗
PubMed1989

Histological classification of synovitis.

A simple system of synovitis classification is proposed based on histomorphological evaluation of 400 random specimens of synovial tissue. It discriminates between two types of diagnosis: 1) one with a high degree of information, being called C-synovitis (synovitis with a characteristic or diagnostic histomorphological pattern), where the clinical diagnosis can be predicted on the basis of the histomorphological pattern, 2) a second type of diagnosis with a lower degree of information, being called NC-synovitis (synovitis with non-characteristic or non-diagnostic morphological findings in the synovial membrane). We subdivided NC-synovitis into 4 subgroups and determined the RA-probability of each subgroup in order to permit clinical use of the proposed histomorphological classification. In addition, by using immunoperoxidase staining methods for immunoglobulins the subgroups were shown to have different quantities of extravasally deposited immunoglobulins in the synovial membrane. The subgroup with the highest RA-probability also turned out to have the highest amount of extravasally deposited immunoglobulins.

Fibroblasts↗
PubMed1988

Increased expression of Thomsen-Friedenreich antigens during tumor progression in breast cancer patients.

Paraffin-embedded tissue sections of primary tumors and lymph node metastases of 80 breast cancer patients were tested for the expression of Thomsen-Friedenreich (TF) antigens with the aid of a monoclonal IgM antibody (49H8) highly specific for phenyl-beta-galactoside. TF antigens were not expressed in 16 different normal tissues with the exception of some structures in the kidney. In tumor cells, two types of antigen expression were found; namely, cryptic and exposed. From stage T1/No to stages T2-4/N1,2 the number of cases expressing high amounts of TF antigens increased from 9% (2/22) to 22% (4/18) while the percentage of patients with low intensity of antibody binding was reduced from 59% (13/22) to 39% (7/18). The total amount of TF-positive primary tumors at stages T2-4/No increased from 42% (8/19) to 69% (18/26) when lymph nodes were infiltrated (T2-4/N1,2). At this stage 80% (21/26) of the patients with lymph node infiltration carried TF antigens in the nodes. The distribution of antigens was heterogeneous among the tumor cells and was expressed mainly in an apical or luminal position. The increased expression of antigens was attributed to exposed TF antigens, while cryptic antigens remained constant. When primary tumors expressed exposed TF antigens, the corresponding lymph nodes also contained exposed antigen. The same was true for the cryptic antigen. The data demonstrate an increase in the intensity of TF antigen expression during tumor progression and a spread of TF-positive tumor cells into the axillary lymph nodes with an increasing number of breast cancer patients being TF-positive at this stage.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1988

Immunophotometric quantification of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis.

The amount of extravascular immunoglobulin deposits in the synovial membrane of patients with osteoarthritis and rheumatoid arthritis was studied in comparison with that of patients suffering from non-joint diseases. Immunoglobulin deposits were immunostained using the three-layer immunoperoxidase method. The staining results were quantified with the help of a microscope photometer connected with a scanning stage. Several experiments involving artificial test substrates, diseases with allegedly increased extravascular deposits or diseases not exhibiting extravascular deposits of immunoglobulins are used to validate the described microspectrophotometrical approach for measuring extravascular immunoglobulin deposits. The scanning photometry demonstrates significantly higher amount of extravascular immunoglobulin deposits in rheumatoid arthritis as compared with osteoarthritis and non-joint diseases.

Arthritis, Rheumatoid↗
PubMed1988

Use of lectin-immunohistochemistry in joint diseases.

The lectin binding pattern of the inflamed synovial tissue of patients with OA (osteoarthritis) and RA (rheumatoid arthritis) were examined with 18 different lectins. The synovial lining cells are heavily stained by PHA-L, WGA, PNA, Con A, MPA and RCA. In the subsynovial layer, WGA, PHA-L and RCA were found to be fairly good monocytic markers. The lectin binding pattern of the lectins under study, however, failed to discriminate between patients with OA and RA.

Arthritis↗
PubMed1988

[Treatment by external drainage of perirenal abscess in AIDS].

Fever of undetermined origin in a young white heroin addict with a positive HIV (human immunodeficiency virus), serology was secondary to a staphylococcal perinephric abscess. Ultrasound examination and tomodensitometry allowed the diagnostic. Percutaneous drainage led to complete recovery. Interventional radiology associated with antibiotherapy appears to be an alternative to surgery in the treatment of perinephric abscesses.

Abscess↗
PubMed1987

Immunohistochemical localisation of arginase in human liver using monoclonal antibodies against human liver arginase.

Monoclonal antibodies against human liver arginase were raised in order to determine the exact distribution of arginase in human liver using a modified indirect unlabelled immunoperoxidase method. In normal human liver specific immunohistochemical staining was found in the cytoplasm of hepatocytes. Portal components (bile ducts and veins) and fibrous tissue were non-reactive, while erythrocytes were slightly positive. The specificity of the immunological reaction was confirmed by control tests. Spectrophotometry was used to quantitate the immunohistochemical reaction product, and the results indicated that arginase is homogeneously distributed in the liver lobule.

Animals↗
PubMed1987

[Detailed structural analysis of the petrous bone using high-resolution thin-layer computerized tomography. 1. Sensitivity of substructural demonstration using a standardized study technic].

High-resolution CT, using fine sections, is a valuable method for radiological examination of the petrous bone. A standardized method of examination using the orbitomeatal and coronary plane is adequate for detailed screening of the petrous bone and shows almost all structures with high accuracy. The sensitivity of the method for showing 35 sub-structures of the inner and middle ear was investigated by examining 40 petrous bones which were normal clinically and radiologically, using the orbito-meatal and coronary planes. The radiation dose to the lens is less than from pluri-directional tomography. For special problems and for demonstrating very small structures, cuts may have to be performed in other planes, suitable for the required topography.

Ear, Middle↗
PubMed1987

[Value of various radiological study results in the follow-up of Camurati-Engelmann disease].

Camurati-Engelmann disease is a rare progressive bone dysplasia; involvement of the skull base can lead to deafness, vestibular disturbances, facial paralysis and damage to the optic nerves. Treatment with corticosteroids, calcitonin and diphosphonates promises only very limited success. Conservative treatment of compression of the cranial nerves is almost ineffective, but the aim of surgical treatment is decompression of involved nerves. The differential diagnosis depends on radiological findings and clinical symptoms. Follow-up depends on radiographic examination and skeletal scintigraphy for showing the extent of the disease. CT may help in demonstrating compression of cranial nerves and define the indications for surgical decompression.

Adult↗
PubMed1987

Brachytherapy of tumour recurrences in the region of the pharynx and oral cavity by means of a remote-controlled afterloading technique.

In the period 1981-86, 22 patients with local inoperable tumour recurrences in the mouth and pharynx area had endocavitary contact therapy using a remote-controlled afterloading system at the Radiotherapy Centre of the University of Heidelberg. All patients had had previous surgery and external-beam radiation; some had also received chemotherapy. The technique is described and preliminary results are discussed.

Aged↗
PubMed1987

[Scintigraphic localization of the liver and kidneys for abdominal radiotherapy: methods, accuracy and comparison with the ultrasonic procedure].

The exposure of liver and kidney has to be avoided when irradiation fields for radiotherapy of the abdomen are planned and adjusted, unless these organs themselves are the target of therapeutic procedure. A scintigraphic method is presented which allows to transfer the site and borderlines of the organs to the body surface taking exactly into account the irradiation position. A precise transfer of site, size, and shape of the organs facilitates the adjustment of irradiation fields or the positioning of satellite field stops. Scintigraphic organ localization is performed by means of static liver or kidney scintigraphy with suitable radiocolloids for the liver and 99mTc DMSA for the kidneys. The scintigraphic procedure meets all practical requirements regarding the precision of organ localization and shows evident advantages compared to X-ray and ultrasonographic methods. It can be performed at every department of nuclear medicine without preparing the patient and is not influenced by intestinal air artefacts, skeletal parts, obesity, or dystopic organ sites. The results of scintigraphic and ultrasonographic kidney localisation are compared in 15 patients. The scintigraphic procedure has shown to be superior, even with regard to precision and possibility of variable projections to the body surface.

Abdominal Neoplasms↗
PubMed1987

[Myeloblastic myoma of the tongue].

Usually the myeloblastoma of the tongue (Abrikossoff's tumour) is a benign tumour. Two cases are reported that differ from those described in the literature in respect of findings, course and histology. Particular reference is made to the malignant nature of this tumour.

Adolescent↗