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P Fritz

Publications and source records attributed to P Fritz.

At least 109 records · Page 6Linked to original sources
PubMed1992

Morphometric analysis of the angioarchitecture of the synovial membrane in rheumatoid arthritis and osteoarthritis.

Using three different immunohistochemical methods we measured the number of vessels, vessel area and diameter and their form factor in the synovial membrane of 102 patients suffering from different joint disease. The variables were evaluated by means of immunomorphometric analysis. We found UEA (Ulex europeus) immunostaining to be the optimal method for quantification of data characterizing the vasculature of the synovial membrane. Irrespective of causes of the given joint disease, we found increases in the number of vessels, vessel perimeter, vessel area and the product of the number of vessels and vessel area over and against the control group (patients without arthritis). Consequences are discussed regarding local bioavailability of medicaments in the synovial membrane.

Arthritis, Rheumatoid↗
PubMed1991

[The localization of colorectal polyps and carcinomas in relation to their size and the histological findings].

Location, size and histological appearance of colorectal neoplasms were examined retrospectively. A total of 1,357 polyps (including small ones, diameter below 0.5 cm) were found in 1,022 of 3,057 coloscopies. The coloscopies were indicated by findings on preceding radiological or endoscopic examinations (48%), occult blood in stool (18%), during follow-up after polypectomy (21%), and after colorectal carcinoma (13%). A single polyp was found in 718 cases (70%), two to four in 221 (26%), more than five in 33 (4%), 1,106 polyps (61%) were found on high coloscopy; 380 of these (38%) were located proximal to the left flexure. Among the 1,230 polyps examined histologically 907 (74%) were adenomas (494 tubular [54%], 379 tubulovillous [42%], 34 villous [4%]). 251 (28%) of the adenomatous polyps and 49 (32%) of the 151 carcinomas were located proximal to the left flexure. The incidence of moderately severe to severe dysplasias increased with increasing diameter of the polyps: 55% of those smaller than 0.5 cm were adenomas. 70% of polyps with a diameter over 1.0 cm were tubulovillous or villous adenomas. The findings confirm that there is a high incidence of polyps proximal to the left flexure. A complete coloscopy should therefore be done as a matter of course.

Age Factors↗
PubMed1991

Palliation of esophageal cancer--operative resection versus laser and afterloading therapy.

Since 1985, 71 patients with end-stage esophageal cancer have been treated either by surgical (n = 26) or endoscopic laser palliation (n = 45). In 16 of 45 patients treated by endoscopy, additional radiotherapy (extrenal and endoluminal irradiation) was performed. Surgery and Nd:YAG recanalization were initially effective in removing the malignant obstruction in 80% of cases. There were no significant differences in survival in either group. The stenosis-free interval was longer in patients who underwent surgery: 24 weeks; minimal stenosis-free interval: 20 weeks. Local recurrences occurred earlier in the endoscopic study group (mean survival: 36 weeks; minimal stenosis-free interval: 20 weeks). Most stenoses successfully underwent further laser treatment. Although only 35% of patients treated endoscopically underwent additional afterloading therapy, this treatment appears to prolong palliation (mean survival: 38 weeks; minimal stenosis-free interval: 36 weeks). A few patients bled after endoscopy and were treated conservatively. The most important complication in the afterloading group was esophagobronchial perforation, which caused one death in our series. Transient pulmonary problems were the most common complication (31%) in the surgical group with a hospital mortality of 19%. Overall, the improvement in the quality of life after surgery was better. However, our results show that Nd:YAG recanalization and afterloading therapy are effective therapeutic alternatives in patients unfit for surgery.

Brachytherapy↗
PubMed1991

A new applicator, positionable to the center of tracheobronchial lumen for HDR-IR-192-afterloading of tracheobronchial tumors.

A new bronchial applicator for afterloading irradiation is introduced which can be positioned to the center of the tracheobronchial lumen. The central position in the lumen leads to a clear improvement of dose distribution. The applicator is built on the principle of a coaxial tube. Parts of the outer cover can be expanded to baskets and effect a distance of the radiation source from the bronchial mucosa or tumor surface, and at the same time, expend a relief of extreme contact doses. No obstruction of the respiratory system through the positioning device will be caused. The positionable bronchial applicator seems to be suitable for reducing complications caused through high contact doses and irregular dose distributions and may be able to improve the results of endoluminal radiotherapy.

Brachytherapy↗
PubMed1991

Lack of efficacy of d-propranolol in neuroleptic-induced akathisia.

d-Propranolol lacks clinically significant beta-adrenergic receptor blocking properties, but has the same membrane stabilizing effects as racemic (d,l) propranolol. To assess the role of beta-blockade versus membrane stabilization or other shared nonspecific effects in the therapeutic action of propranolol in neuroleptic-induced akathisia (NIA) we treated 11 patients with NIA in a crossover, double-blind study of d-propranolol versus placebo. Akathisia scores were unchanged after both d-propranolol and placebo. Eight patients were subsequently treated in a nonblind manner with racemic propranolol, with a significant reduction in akathisia scores. These findings suggest that beta-blockade, not membrane stabilization or other shared nonspecific effects, contributes to the efficacy of propranolol in NIA.

Adult↗
PubMed1991

Effect of alcohol-feeding on IgA-producing immunocytes in the small intestine of rats with and without jejunoileal bypass.

Alcohol-feeding to rats subjected to jejunoileal bypass operation has been shown to lead to marked liver injury (fatty liver, necrosis and inflammation). This study investigated the influence of alcohol-feeding over a period of 3 months on the number of IgA-producing immunocytes and the villus surface area in various sections of the small intestine of rats subjected to a jejunoileal bypass or a sham operation. A jejunoileal bypass in animals receiving the control diet led to a decrease in the number of IgA-producing immunocytes in the duodenum and ileum, but not in the bypassed (blind) loop of the jejunum. In animals subjected to a jejunoileal bypass, alcohol-feeding led to an increase in the number of IgA-producing immunocytes in the duodenum and the bypassed jejunal loop as compared with animals with a jejunoileal bypass receiving the control diet. Among the animals with a jejunoileal bypass fed the control diet, the villus surface area within the duodenum and ileum increased as compared with the groups of sham-operated rats. The feeding of alcohol prevented this increase in the villus surface area in animals with a jejunoileal bypass. The increase in the number of IgA-producing immunocytes induced by alcohol in the animals with a jejunoileal bypass, in the duodenum and bypassed jejunum, supports the assumption of a change in antigen uptake in these parts of the small intestine, brought about by alcohol.

Animals↗
PubMed1990

Transfer of adriamycin resistance by fusion of Mr 170,000 P-glycoprotein to the plasma membrane of sensitive cells.

One of the characteristics of multidrug-resistant mammalian cells is the presence of a glycoprotein of approximately Mr 170,000 in their cell membrane. Termed P-glycoprotein, this glycoprotein is thought to be the gene product of an amplified gene cloned and sequenced from a cell line (LZ) which is highly resistant to Adriamycin and cross-resistant to actinomycin D, colchicine, and vincristine. Resistance to Adriamycin has been induced in sensitive cells by chromosome or gene transfer. We now show that P-glycoprotein isolated from LZ cells and fused to sensitive V79 Chinese hamster cells renders the latter transiently resistant to Adriamycin. Incorporation of P-glycoprotein was confirmed by immunoperoxidase staining of fusion products following treatment with antibody to P-glycoprotein and by Western blots of membrane preparations from fusion products. These results suggest that P-glycoprotein is one of the important factors in the expression of Adriamycin resistance and provide added confirmation that it may be the important product of gene amplification in multidrug-resistant cells. The results also suggest that the cell membrane may be one of several targets for Adriamycin cytotoxicity and that P-glycoprotein may be a binding site for Adriamycin, rendering the latter ineffective in registering sufficient membrane damage for cell killing.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1990

Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients with rheumatoid arthritis and related diseases.

Using the technique of two-dimensional (2D) electrophoresis with consecutive silver staining, we investigated samples of serum, synovial fluid and synovial tissue obtained from 19 patients suffering from rheumatoid arthritis (RA) or non-RA arthritis. From these experiments we have drawn the following conclusions. 2D electrophoresis of serum, synovial fluid and synovial tissue extracts taken from patients suffering from joint diseases is a reproducible method. Repeated runs of the same sample reveal an essentially constant protein spot pattern. The time period between surgery and tissue preparation did not influence the number of protein spots when less than 15 h was involved. The protein spot number is always lower in synovial fluid than in either synovial tissue or serum in RA and non-RA patients. The mean value for the number of spots is 68 for the inflamed tissue irrespective of the cause of arthritis (RA and non-RA group taken together) and 47 for the control group. This difference is significant. We were able to definitely identify 7 spots in the tissue extract. We did not find RA-specific protein spots in either serum, synovial fluid or tissue extracts from the synovial membrane. The only significant difference between RA patients and either non-RA or control group patients concerning the protein spot pattern is the increased size of the immunoglobulin spot (mainly IgG) in RA. In addition, we discuss possible reasons for failure of the 2D electrophoresis technique to detect disease-specific protein patterns.

Adult↗
PubMed1990

The neo-synovial membrane of patients with active rheumatoid arthritis at resynovectomy. Histomorphological and immunohistological evaluations.

Neo-synovial membranes, which formed after "primary" synovectomy in 21 patients with rheumatoid arthritis (RA), were studied at resynovectomy. The clinical, histomorphological, and immunohistological data were compared with data derived from "primary" synovial membranes from RA and osteoarthritis (OA) patients. The clinical data suggest a less active rheumatoid inflammatory response after synovectomy. Histomorphologicaly, the synovitis in resynovectomized neosynovial membranes of RA revealed no qualitative differences when compared with synovitis in the "primary" synovium. However, the degree of the inflammatory rection evaluated by the different parameters was found to be distinctly lower. The immunohistological data correlated with these findings.

Adult↗
PubMed1990

[Interpretation of the estrogen receptor content of breast cancer].

The biochemical analysis of estrogen receptor (ER) content, using the DCC (dextran-coated charcoal) method, was compared with different plotting methods of the estrogen-receptor immunocytochemical assay (ER-ICA) in 80 primary breast cancers including 9 metastases under routine conditions. It was evident, that the determined content of estrogen receptors depends on the technique of measurement, as well as the microscopic organizations of the individual carcinomas and should be interpreted in respect of their content of stroma and if possible of tumour heterogeneity.

Antibodies, Monoclonal↗
PubMed1990

Uremic pruritus in patients on hemodialysis or continuous ambulatory peritoneal dialysis (CAPD). The role of plasma histamine and skin mast cells.

Fifty-four uremic patients (26 patients were maintained on continuous ambulatory peritoneal dialysis [CAPD] and 28 on hemodialysis [HD]) were screened for pruritus and plasma histamine. 50% of the patients on CAPD and 64.3% of patients on HD complained of current itching. The extent of itch, assessed by a score, was not significantly different between HD and CAPD patients. Plasma histamine levels showed no significant difference between CAPD and HD patients and no correlation between plasma histamine level and the extent of pruritus could be demonstrated. In six patients on CAPD and 9 patients on HD plasma histamine levels were determined before and three months after initiation of dialysis treatment; no substantial change could be observed. In some patients skin biopsies were obtained from the gluteal region in order to determine the number of skin mast cells. The number of mast cells did not show a significant difference between controls (n = 12), uremic patients before initiation of dialysis treatment (n = 8), patients on CAPD (n = 11) and patients on HD (n = 13). There was no relationship between the level of plasma histamine, the number of skin mast cells and the extent of pruritus in uremic patients.

Adult↗
PubMed1990

Application of the chromogenic reaction to conventional silver staining, the Ag-NOR staining and the silver-intensified immunogold technique.

The principle of the chromogenic reaction and the transformation of "black and white" histochemical staining results or immunohistochemical signals to coloured microscopic images is described. The chromogenic reaction was optimized and is, so far, possible with either cyan-blue or magenta-red reaction products. The application of the chromogenic reaction to conventional silver stain was optimal in the Lendrum staining resulting in red or blue stained reticulin fibres. The Ag-NOR staining of the nucleolus organizing region (NOR) could be transformed by the same reaction to coloured reaction products as well as the silver-intensified immunogold technique in immunocytochemistry.

Arthritis, Rheumatoid↗
PubMed1990

Application of a new electrophoresis technique (2D cryostat section electrophoresis) to synovial tissue of RA patients and comparison with immunohistochemical staining methods.

We describe a new two dimensional electrophoresis technique which is based on the combination of cryostat section technology and IEF- and SDS-gel electrophoresis. The optimal conditions for two dimensional cryostat section electrophoresis are investigated. The application of this technique to synovial membranes of patients suffering from rheumatoid arthritis and osteoarthritis is described.

Arthritis, Rheumatoid↗
PubMed1989

Immunocytochemical detection of sulphonylated DNA in tissue sections. An alternative to Feulgen staining of DNA.

We report here on a new sensitive and highly specific DNA staining technique which we have called sulpho-DNA staining. DNA staining is based on a sulphonylation reaction of 2'-deoxycytidine or cytidine that takes place in the 6th position of cytosine with ensuing immunodetection of the sulphonylated DNA. The specificity of DNA staining is introduced by the use of an antibody recognizing only modified DNA but not modified RNA, by recourse to an additional acid hydrolysis step which destroys RNA but not DNA. We describe here the optimal conditions for the sulphonylation of DNA using O-methylhydroxylamine and metabisulphite as reactants. The new DNA stain labels all nuclei in either normal human tissue or in tumor cells. For nuclear DNA the staining signal is higher for the sulpho-DNA staining than for the Feulgen staining for nuclear DNA. This new DNA staining technique is suitable for use on tissue sections as well as on cytosmears.

Animals↗
PubMed1989

Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.

Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.

Cells, Cultured↗
PubMed1989

[Detection of muscle, vein and artery infiltrations of cervical lymphatic metastases].

In a prospective study, 15 patients with squamous cell carcinomas of head and neck and clinical fixed cervical lymph node metastases were examined to determine the value of computed tomography, magnetic resonance imaging and high resolution real time ultrasonography in preoperative recognition of muscle and vessel infiltration. The preoperative findings were compared with the microscopic results of neck specimens and the operative reports. For each diagnostic method sensitivity, specificity, and effectivity were calculated. Ultrasonography, magnetic resonance imaging and computed tomography can assess muscle infiltrations as well. Metastatic infiltrations of carotid arteries are best recognised by ultrasonography and magnetic resonance imaging. Both methods are more valuable than computed tomography. All methods are unsuccessful in detecting infiltrations of the jugular vein. In conclusion, metastatic infiltrations of surrounding tissue, as described by imaging methods, are rarely confirmed by histological results.

Carcinoma, Squamous Cell↗
PubMed1989

[Fine structure analysis of the petrous bone using high-resolution thin-layer computed tomography. 2: Place and success of CT in comparison with conventional x-ray diagnosis of petrous bone fractures].

Thirty patients with suspected petrous fractures following trauma were examined by high resolution CT and by conventional radiography. CT proved the most sensitive method for demonstrating petrous fractures and their complications. CT increased the rate of diagnosis by 17%. All fractures seen on CT could be classified, but accurate classification on the basis of conventional radiographs was only possible in 52% of cases. High resolution CT is superior in demonstrating damage to the labyrinth and the ossicles. Indications for conventional tomography are confined to atypical fractures with a course unsuitable for CT.

Humans↗