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Biomedical subjects

P Fric

Publications and source records attributed to P Fric.

At least 55 records · Page 3Linked to original sources
PubMed1990

Therapeutic methods of digestive endoscopy in persons of advanced age.

Towards the end of 1988, 85 out of 170 patients indicated for endoscopic papillotomy (EPT) were over 65 years of age, and, of these, 54% were 75 and more. EPT was successful in 90.6% of those over 65. Total morbidity was 4.7% and mortality 1.2%. Endoscopic colorectal polypectomy (EPE) was performed during the same period in 591 subjects. 143 of them (24.2%) were over 65 and had a total of 284 polyps removed. Almost half those polyps were more than 10 mm in diameter. Adenomas of the right colon were at least 3 times as frequent as in asymptomatic subjects aged 45-60 years revealed by screening for colorectal neoplasms in HaemocultR-positive subjects. Particular attention was devoted to patients with malignant polyps and with very broadly sessile adenomas--both could definitively be treated with the endoscopic method. EPT is often the only possible therapy for elderly and high-risk patients. Every colorectal polyp should be evaluated by endoscopy, and EPE, especially in patients of advanced age, is the method of choice when technically feasible.

Aged↗
PubMed1990

[Endoscopic colorectal polypectomy in children and adolescents].

Endoscopic polypectomy was performed in a total of 22 children and two adolescents aged 3 to 17 years. The most frequent symptom leading to examination was enterorrhagia. During 31 colposcopic examinations a total of 113 polyps was removed. From the histological aspect in all patients juvenile polyps were involved, only in one patient with familial polyposis a tubular adenoma was detected. No complications were observed. Endoscopic polypectomy from the large intestine and rectum in children is a safe and satisfactory method which replaces the standard surgical approach and is much more sparing for the child.

Adolescent↗
PubMed1990

[Expression of CA 19-9 in tubular and tubulovillous adenomas of the descending and sigmoid colon and the rectum with respect to morphologic differentiation characteristics and the adenoma-carcinoma sequence].

Expression of CA19-9 was studied in 29 adenomas and 6 adenocarcinomas of the distal colon in rectum. The authors did not find an unequivocal correlation between expression of CA19-9 and the morphological differentiation. Expression of CA19-9 was recorded twice in epithelia of normal mucosa.

Adenocarcinoma↗
PubMed1989

[Therapeutic methods of digestive tract endoscopy in older persons].

By the end of 1988 of 170 patients indicated for endoscopic papillotomy (EPT) 85 subjects were above 65 years of age and of these 54% were 75 years old or above that age. EPT was successful in 90.6% of subjects above 65 years of age. The total morbidity was 4.7% and the mortality 1.2%. Endoscopic colorectal polypectomy (EPE) was performed during the same period in 591 subjects. Of these 143 (24.2%) were above 65 years and in these subjects 284 polyps were removed. Almost half the polyps in these patients had a diameter of more than 10 mm, and adenomas of the right colon were at least three times more frequent than in asymptomatic subjects aged 45-60 years revealed by screening of colorectal neoplasms in HaemocultR-positive subjects. Special attention was devoted to patients with malignant polyps and with very broadly attached adenomas of the left colon. Almost three quarters of patients with very broadly attached adenomas could be definitely treated by the endoscopic method. EPT is in many instances the only possible therapeutic approach in old and risk patients. Every colorectal polyp should be evaluated by endoscopy and EPE is in particular in subjects of advanced age the method of choice, when technically feasible.

Aged↗
PubMed1989

[Energy support with parenteral nutrition in patients with chronic intestinal inflammation].

Parenteral nutrition (PN) was administered to 24 patients with chronic enteritis. Their mean age was 37 years (range 17 to 69 years). Seventeen times Crohn's disease was involved, five times ulcerative colitis and twice postprandial enteritis. The mean period of PN was 24.6 +/- 12.9 days and its energy value was 7.79 +/- 1.63 MJ/24 hours. Some patients had a restricted oral intake, on average 5 MJ/24 hours. The energy output at rest assessed by indirect calorimetry was 7.62 +/- 1.06 MJ/24 hours. Along with PN the patients had aimed antiinflammatory treatment. In the majority of patients clinical improvement was recorded. The nutritional status improved in 13 patients, was not affected in 9 and in two it deteriorated. The improvement of the nutritional status was not always associated with regression of manifestations of the local enteritis. In particular affections of the rectum in Crohn's disease were resistant to treatment and the position changed after a derivation stoma operation.

Adolescent↗
PubMed1989

[The effect of Sinecal fiber on compensation in type 2 diabetics].

The authors investigated in 28 type 2 diabetics the effect of addition of 21 g Sinecal fibre to the normal diet on the glucose and lipid metabolism. The value of C,peptide was significantly influenced after breakfast and a single dose of Sinecal. The other investigated parameters (blood sugar on fasting and after a meal, glycosuria, glycosylated proteins, C-peptide on fasting and after a meal, triacylglycerol and cholesterol) were not influenced by a single nor by long-term intake of Sinecal. Further development of fibre preparations with better tolerance and a greater effect on the glucose metabolism is desirable.

Aged↗
PubMed1989

Enteropathy induced in young rats by feeding with gliadin--similarity with coeliac disease.

Young rats (10- and 21-day-old) were given gliadin by intragastric application, in the form of a 5% and 0.5% solution. A 5% gliadin (1 ml) was intolerable for young rats, destroying the structure of their jejunal mucosa. A 0.5% gliadin (1 ml) was well tolerated by 21-day-old animals and caused no changes in enterocytes. Repeated administration of 0.5% gliadin on days 0 and 17 caused an increased lymphocyte infiltration in the lamina propria and an increase in the number of cells producing IgA immunoglobulins. Repeated intragastric administration of 0.5% gliadin until 1 month of age, followed by a single administration of 0.5% gliadin at 2 months, caused shortening of the jejunal villi and changes in apical enterocytes. The most pronounced immunological changes were observed in these repeatedly treated animals. Increased numbers of lymphocytes producing IgG in the lamina propria were found and the intestinal contents of these animals exhibited an increased level of IgG and IgA anti-gliadin antibodies when compared to control rats. The results indicate that some morphological and immunological changes observed in young rats fed with gliadin resemble those observed in patients with coeliac disease. The described experimental model is supposed to be suitable for studying the pathogenetic aspects of human coeliac disease.

Age Factors↗
PubMed1988

Isolation and analysis of peptidic fragments of alpha-gliadin using reversed-phase high-performance liquid chromatography.

Peptidic fragments of alpha-gliadin were obtained by peptic-tryptic-pancreatic (PTP) digestion of the alpha-gliadin fraction isolated by ion-exchange chromatography on a sulphopropyl-Sephadex C-50 column. The proteolytic digest was fractionated by ultrafiltration into three subfractions, PTPa1-PTPa3. The subfraction PTPa2 was then analysed and individual peaks were separated using reversed-phase high-performance liquid chromatography (RP-HPLC) using a gradient of acetonitrile in 0.1% trifluoroacetic acid and a Separon SGX-C18 sorbent. A 100-mg amount of the PTPa2 subfraction was separated in a single analysis by preparative RP-HPLC and twenty peaks were obtained for further characterization. The molecular mass in range 300-3000 was established for individual peptidic fragments by gel-permeation chromatography on a TSK-G2000 SW column.

Chromatography, High Pressure Liquid↗
PubMed1988

Monoclonal antibodies reacting with gliadin as tools for assessing antigenic structure responsible for exacerbation of celiac disease.

Monoclonal antibodies reactive with gliadin were prepared by fusion of spleen cells isolated from gliadin-immunized BALB/c mice with myeloma cells. The reactivity of mAbs with different preparations of gliadin and their enzymatic digest were measured using ELISA method. The mAb produced by GL 1 clone was shown to react preferentially with alpha-gliadin and its enzymatic digest.

Antibodies, Monoclonal↗
PubMed1988

Absence of cytomegalovirus, Epstein-Barr virus, and papillomavirus DNA from adenoma and adenocarcinoma of the colon.

Biopsy specimens from 13 patients with adenocarcinoma of the colon and from 10 patients with endoscopic polypectomies for colon adenoma were examined for the presence of the DNA of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human papillomavirus (HPV) types 2, 6, 16 and 18. The specific activities of viral DNA probes obtained by nick--translation ranged from 10(7) to 10(8) cpm/micrograms DNA. By Southern blot hybridization with an estimated sensitivity of 10 pg virus DNA which corresponded to 0.05 virus genome equivalents per cell we failed to detect any virus DNA in the biopsy material tested.

Adenocarcinoma↗
PubMed1986

Subpopulations of circulating lymphocytes in adults with coeliac disease.

Circulating lymphocytes were enumerated in 25 patients with coeliac disease and in 24 healthy donors by immunofluorescent staining using monoclonal antibodies for T cell surface phenotypic markers T11 (mature), T4 (helper) and T8 (suppressor) or polyvalent antisera for surface immunoglobulins (B cells). Proportions of peripheral T and B cells and percentages and ratio of T cell subsets in coeliac disease were not significantly different from those in controls. Individual ratios of helper to suppressor T cells did not correlate with disease activity or with keeping the diet.

Adult↗