Biomedical subjects

P Fantazzini

Publications and source records attributed to P Fantazzini.

30 records · Page 2Linked to original sources

PubMedDate not supplied

1H spin-lattice relaxation parameters in the length of human intestine resected for cancer.

1H spin-lattice relaxation curves were acquired for samples of intestinal adenocarcinoma (B) and of uninvolved tissue at the upper (A) and lower (C) resection margin of lengths of intestine taken at surgery from 20 patients. Each sample showed a wide distribution of relaxation times with the order of 90% of the signal in a single peak at long times. Several different single-parameter relaxation times computed from discrete-exponential analysis showed that most of the relaxation times for C and B are in the upper two-thirds of the range of times for A. The mean time for the tumor is about 10% longer (with p < 0.01) than for the upper resection margin. The difference between the tumor and the lower resection margin is not significant. Distribution width parameters associated with A and C were significantly larger than those associated with the tumors. Two-exponential fits indicate that the fast-relaxing component represents a smaller signal fraction for the tumor B than for A or C.

Adenocarcinoma↗

PubMedDate not supplied

Variations of the 1H spin-lattice relaxation in a fatty liver model as compared to normal liver.

We report the results of an in vitro study on ethionine-injected rat liver (EI) and on normal rat liver (C) performed analyzing with iterative fitting procedures the 1H spin-lattice relaxation curves detected by IR pulse sequence at 20 MHz and at 37 degrees C on fresh excised samples. Single-exponential functions did not adequately describe the experimental curves both in EI and in C group. The analysis of the curves by two-exponential hypothesis showed a small portion of the signal characterizable by a time constant of about 80 ms, common both to EI and to C samples. A slowing of about 30% in the relaxation characterized the remaining portion of the curve (90-95%) in EI as compared to C samples. The hypothesis that the 1H of the triglycerides vacuoles present in EI livers had a relaxation curve additional to the remaining signal was checked by three-exponential analysis. The results were not in contrast with the known value of the triglycerides percentage content and with the spin-lattice relaxation time of the -CH2 group 1H obtained in different experimental conditions in the same fatty liver model. The negative results of the three-exponential analysis on normal liver curves as well as the favorable controls performed to test the analysis procedure supported further this hypothesis. The remaining signal after subtraction of the triglycerides contribution showed still the small fast portion and the increase of the relaxation time of the major portion (from approximately 300 ms up to approximately 400 ms) as compared to C samples.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMedDate not supplied

Characterisation of crosslinked elastomeric materials by 1H NMR relaxation time distributions.

One of the most critical structural parameters in elastomeric materials is the density of cross-linking between the polymeric chains. This chemical feature greatly affects chain motions and is determinant in controlling mechanical properties of the final product. NMR techniques are widely and efficiently applied to investigation of such materials. In this study we have measured both transverse and longitudinal 1H relaxation times of a series of polybutadiene rubber samples with increasing crosslink density induced by chemical treatment. This approach allowed the observation of T(1) and T(2) decrease with the increase of crosslink density in the samples examined. The data obtained have been analyzed and compared to theoretical models.

Elastomers↗

PubMedDate not supplied

Examples of marginal resolution of NMR relaxation peaks using UPEN and diagnostics.

The multiexponential inversion program UPEN by the authors [J. Magn. Reson. 1998; 132: 65-77; Ibid. 2000;147:273-85] employs negative feedback to a regularization penalty to implement variable smoothing when both sharp and broad features appear on a single distribution of relaxation times. This allows a good fit to relaxation data that correspond to a sum of decaying exponentials plus random noise, but it usually does not give a good fit to data that are distorted by systematic errors from instrument problems, which can cause erroneous "resolution" or erroneous non-resolution of peaks. UPEN provides a series of diagnostic parameters to help identify such data problems that can lead to interpretation errors, and, in particular, to warn when a close call on the resolution or non-resolution of nearby peaks might be questionable. Examples are given from a series of T(2) data sets from desiccated bone samples, with examples where the presence of two peaks is required by good data, examples where the presence of two peaks is negated by good data, and examples where the resolution or non-resolution of peaks cannot be trusted because of instrumental distortions revealed by UPEN diagnostic parameters. It is suggested that processing relaxation data with UPEN in nearly real time could permit retaking data while a sample is still available if the diagnostic parameters show instrumental problems.

Bone and Bones↗

PubMedDate not supplied

From porous media to trabecular bone relaxation analysis: spatial variation of marrow 1H relaxation time distributions detected in vitro by quasi-continuous distribution analysis.

Quasi-continuous distributions of T(1) and T(2) of 1H nuclei were analyzed in vitro at 20MHz on some twenty fresh bone samples of pig femur. Large numbers of data points allowed a detailed investigation. Relaxation data were inverted by UPEN (Uniform PENalty inversion). In all samples the widths of the distributions, covering more than two decades, are not even close to being compatible with single exponential components. Moreover, the T(1) and T(2) distributions show enough character to distinguish the samples. We observe a spatial variation of these characteristics and in particular a second peak centered at 500-600 ms appearing in some proximal femur samples. The quasi-continuous distribution allows one to correlate the water content of the sample with parts of the distributions in specific time ranges. The signal fraction with T(1) values longer than a cutoff time of about 170 ms is in very good agreement with the water content of the samples and is significantly larger in the group of samples cored from proximal femur. Also T(2) distributions differentiate the samples, and the signal fraction with T(2) shorter than about 30 ms is significantly larger in the group of distal femur samples.

Animals↗

PubMedDate not supplied

Combined MR-relaxation and MR-cryoporometry in the study of bone microstructure.

MR-Relaxation (MRR) of 1H nuclei and MR-Cryoporometry (MRC) are combined to assess their feasibility and their potential in the study of bone microstructure. In principle, both techniques are able to give information on the structure of the pore space confining the fluids. Cow femur samples were carefully cored and cleaned in order to remove the natural fluids inside. For MRR analysis quasi-continuous distributions of T(1) and T(2) were obtained on samples fully saturated with water. Cyclohexane was used as a saturating fluid for MRC analysis. All T(1) and T(2) quasi-continuous distributions of water confined in bone samples are more than three decades wide, showing sufficient details to differentiate the samples. Pore size distributions obtained by MRC also differentiate the samples showing different characteristics of the pore space structure in the range of the highest sensitivity of the method (typically 3 to 100 nm, mesopore range). In particular, in samples where MRR shows a large fraction of signal with relaxation times below 10(2) ms, MRC indicates a large fraction of pore volume with pore sizes in the mesopore range.

Animals↗

PubMedDate not supplied

Effects of hydrophobic treatments of stone on pore water studied by continuous distribution analysis of NMR relaxation times.

The effects of protective hydrophobic products applied to porous media such as stone or mortar vary greatly with the product, the porous medium, and the mode of application. Nuclear Magnetic Resonance (NMR) measurements on fluids in the pore spaces of both treated and untreated samples can give information on the contact of the fluid with the internal surfaces, which is affected by all the above factors. Continuous distributions of relaxation times T(1) and T(2) of water in the pores of both synthetic and natural porous media were obtained before and after hydrophobic treatment. The synthetic porous media are ceramic filter materials characterized by narrow distributions of pore dimensions and show that the treatment does not produce large changes in the relaxation times of the water. For three travertine samples most of a long relaxation time component, presumably from the largest pores, remains after treatment, while the amplitude of an intermediate component is greatly reduced. For three pudding-stone samples, treatment leads to a substantial loss from the long component and an even greater loss from the intermediate component.

Calcium Carbonate↗

PubMedDate not supplied

Performance evolution of hydrophobic treatments for stone conservation investigated by MRI.

1H-MRI has been applied to the evaluation of the performances of a hydrophobic polymer (Paraloid B72), widely used for the conservation of monumental buildings and other stone artifacts. By this technique it has been possible to visualize the water diffusion in a treated rock material (Pietra di Lecce, a highly porous Italian biocalcarenite) and then indirectly the spatial distribution of the polymer in the rock. The effects of wetting-drying cycles on the hydrophobic efficacy of the acrylic polymer in the inner layers of the rock were also studied. A notable decrease in the water-repellence inside the stone was detected and attributed to a loss of adhesion of the polymer to the substrate, promoted by the action of water.

Calcium Carbonate↗

PubMedDate not supplied

Improved pore space structure characterization by fusion of relaxation tomography maps.

Quantitative Relaxation Tomography in porous media furnishes maps of internal sections where each pixel represents T1 or T2 of water 1H in the corresponding voxel, so that quantitative information on the pore space structure can be obtained. The porosity can be determined at different length scales by correcting pixel by pixel the signal intensity for T2 decay. Moreover, on the basis of the distribution of T1, the microporosity fraction can be computed, as well as several voxel-average porosities. Since T1 and T2 encode different pieces of information, fusion image techniques can improve the characterization of the pore space, showing simultaneously, on the same image, maps of the two parameters. Examples are given of application to a water-saturated travertine core and to a pig femur. Different kinds of look-up tables were tried by varying two of the three dimensions of the HSV color space in such a way as to optimize both the T1 and T2 contrasts simultaneously.

Animals↗

PubMedDate not supplied

A method for approximating fractional power average relaxation times without inversion of multiexponential relaxation data.

A method is presented for approximating fractional power averages of relaxation times for data equispaced in log time, without the need to invert multiexponential relaxation data. This form of average permits giving emphasis to short or long times depending on the choice of the p value, thus giving the possibility of representing different specific properties of porous media. This method has been tested on a large number of nuclear magnetic resonance (NMR) relaxation measurements in porous samples. This new algorithm appears to be robust with respect to both measurement and computation, and its major advantage is that it does not depend on a particular inversion method. Moreover, it permits a very fast computation.

Algorithms↗

PubMedDate not supplied

Estimates of permeability and irreducible water saturation by means of a new robust computation of fractional power average relaxation times.

In a suite of water-saturated sandstones, we have recently demonstrated that irreducible water saturation can be well estimated using relaxation time only, in the form of any of several "averages" giving more emphasis to short times than does the geometric mean time. The best estimate of permeability came from fits giving more emphasis to slightly longer times. In this paper we present estimates of irreducible water saturation and permeability using approximations (here called Robusta approximation) to the fractional power average relaxation time (Tp)1/p. The advantage of this approximation is that it does not involve previous computation of exponential components; therefore, it does not depend on the choice of the inversion method, and it permits a very fast computation. The Robusta approximation gives some of the best correlations using p = -0.55 for irreducible water saturation and p = +0.18 for permeability.

Fractals↗

PubMedDate not supplied

Examples of uniform-penalty inversion of multiexponential relaxation data.

When multiexponential relaxation data are inverted to give quasi-continuous distributions of relaxation times, the computed distribution is usually smoothed by means of an applied penalty function equal to a coefficient, C, times the integrated square of amplitude, slope, or curvature. When the distribution has a sharp peak and either a broad peak or long tail, smoothing with a fixed coefficient, C, widens the sharp peak and/or breaks up the broad peak or tail into two or more separate peaks. An iterative feedback procedure is used to generate a separate C value for each computed point in such a way as to give roughly equal contributions to the penalty function from each computed point. This permits adequate smoothing of broad features without oversmoothing sharp peaks. Examples are given for artificial data, for nuclear magnetic resonance in fluids in porous media, and for nuclear magnetic resonance in biological tissues.

Computer Simulation↗