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Biomedical subjects

P D Ellner

Publications and source records attributed to P D Ellner.

At least 55 records · Page 3Linked to original sources
PubMed1976

A new medium for the presumptive identification of gram-positive uropathogens.

A significant percentage of urinary tract infections are caused by gram-positive microorganisms. The rapid identification of these uropathogens is important in determining the appropriate treatment of such infections. A selective medium containing colistin sulfate and nalidixic acid (Columbia CNA Agar) was modified by the addition of esculin, ferric ammonium citrate, mannitol and phenol red. The new medium (Esculin-Mannitol Agar) was extensively evaluated as a primary plating medium for urine specimens. Isolates were presumptively identified solely by colonial morphology and reaction of the medium. Presumptive identification of the isolates was confirmed by conventional tests. The accuracy of the presumptive identification indicated that Esculin-Mannitol Agar was useful in the primary plating of urine specimens when employed together with an appropriate medium for the recovery of gram-negative organisms.

Culture Media↗
PubMed1975

Mutagenicity testing with Salmonella typhimurium strains. I. Unusual phenotypes of the tester strains.

Some of the Salmonella typhimurium strains in mutagenesis testing are atypical. Unlike the wild S. typhimurium these do not produce H-2-S, are not agglutinated by S. typhimurium typing sera and on fermentation of carbohydrates they do not produce gas. Thus these tester strains cannot readily be identified as S. typhimurium by standard laboratory procedures. This may cause a problem in the differentiation of tester strains from bacterial contaminants. The possibility exits that these phenotypic traits may affect the response of the strains to mutagens.

Mutagens↗
PubMed1975

Detection of bacteriuria by dip-slide. Routine use in a large general hospital.

The use of a dip-slide (Uricult) procedure for the culture of voided urine specimens in a large general hospital was found to give equivalent results to those obtained by conventional culture technics. There was a significant decrease in the number of unsatisfactory speciments that needed to be repeated. The dip-slide procedure was readily accepted by physicians, nurses and laboratory personnel, and significant savings for the institution were realized.

Bacteria↗
PubMed1975

Differences in susceptibility of Enterobacteriaceae and penicillin-resistant Staphylococcus aureus to tetracycline and minocycline.

Two hundred strains of penicillin-resistant Staphylococcus aureus and 311 isolates of Enterobacteriaceae were compared for their susceptibility to tetracycline and minocycline. Thirteen and one-half percent of the staphylococcal isolates were resistant to tetracycline but susceptible to minocycline. Similarly, 24% of the enterobacterial isolates were found to be tetracycline resistant but susceptible to minocycline. Of a total of 511 recent clinical isolates, 14.5% were susceptible to minocycline but were tetracycline resistant.

Enterobacteriaceae↗
PubMed1974

Comparison of the BACTEC system with blind subculture for the detection of bacteremia.

One thousand blood specimens were cultured in BACTEC vials containing modified Columbia broth in aerobic, anaerobic, and hypertonic formulations. Radiometric readings and subcultures were performed on aerobic and hypertonic vials at 24 h and 7 days, and on anaerobic vials at 48 h and 7 days. Significant numbers of false-positive BACTEC readings were obtained. Although all positive cultures were eventually detected by the BACTEC, approximately 20% of blood specimens yielding positive subcultures at 24 h did not give positive BACTEC readings until 48 h.

Aerobiosis↗
PubMed1974

Distribution of Serratia species in clinical specimens.

Eighty-five strains of Serratia were speciated. The majority were Serratia marcescens and were recovered from a variety of clinical specimens. S. liquefaciens and S. rubidaea were recovered only from sputum.

Anti-Bacterial Agents↗
PubMed1974

Evaluation of the Minitek system for identification of Enterobacteriaceae.

Clinical isolates (869) and stock cultures (35) of Enterobacteriaceae were tested in parallel with the Minitek and conventional systems. The Minitek correctly identified 822 of 904 cultures. When a deoxyribonuclease plate was inoculated along with the Minitek, it was possible to speciate Enterobacteriaceae within 24 h. False-positive hydrogen sulfide reactions were the major fault with this system. Reactions were clear-cut and easy for technologists to read.

Bacteriological Techniques↗
PubMed1973

Recovery and identification of anaerobes: a system suitable for the routine clinical laboratory.

A system for the isolation of anaerobes based upon the use of reducible solid media is described. Plates of reducible media prepared and stored aerobically were reduced before use by incubation in a GasPak jar for 24 h. Clinical specimens for culture were carefully selected. The value of Amies transport medium was confirmed. Selective and nonselective formulations of reducible media were used for primary isolation. Abbreviated identification schemes based in part on gas-liquid chromatography are presented. The suitability of this system for improving the recovery and identification of anaerobes in a routine clinical laboratory is documented.

Anaerobiosis↗