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Biomedical subjects

P Coleman

Publications and source records attributed to P Coleman.

At least 109 records · Page 6Linked to original sources

Radiation dose from radiopharmaceuticals contaminated with molybdenum-99.

Sixteen patients undergoing routine nuclear imaging procedures were injected with 99mTc-labeled radiopharmaceuticals containing 99Mo which exceeded the recommended limit of 1 microCi of 99mMo per mCi of 99mTc. The kinetics of the resulting 99Mo distribution in 14 of these patients were studied over a period of several weeks. The mean biologic half-life [T 1/2b] ranged from about 19.3 days to 11.2 days depending on the model used. Similarly, the mean radiation dose to the liver ranged from approximately 0.02 rad/microCi of 99Mo to 0.05 microCi of 99Mo.

Humans↗

Prostatic abscess: CT and sonographic findings.

The value of CT and sonography in the diagnosis and follow-up of abscesses of the prostate was studied in six patients with this disease. Five had CT alone, one had CT and sonography, and one had sonography only. CT findings included an enlarged gland with nonenhancing fluid-density collections that sometimes were multiseptated or had enhancing rims. Sonographic findings were similar, showing a hypoechoic mass with thick walls. Follow-up examinations after antibiotic therapy (one CT, one sonogram) showed improvement or resolution. In the patients studied, CT and sonography were useful methods to detect and follow the course of prostatic abscess.

Abscess↗

Generation of monoclonal antibodies to human prolactin and applications in solid-phase immunoassays.

Monoclonal antibodies specific to human prolactin were generated by an improved hybridoma technique. Following immunization with hormone conjugated with hemocyanin and cell fusion, hybrid cells were first cultured in a semisolid medium containing methylcellulose and later transferred to liquid medium for subculture. Out of 1500 colonies that were initially recovered in one single cell fusion experiment, 300 were shown to exhibit affinity to human prolactin by enzyme-linked immunosorbent assay and radioimmunoassay. Finally, nine monoclonal antibodies having high affinity and specificity to human prolactin were selected for further evaluations. From the results of a cross-matching procedure, one pair of antibodies reacting with discrete antigenic determinants of human prolactin was identified. Using a pair of monoclonal antibodies, the solid phase sandwich immunoradiometric assay and enzyme immunoassay were designed. The sensitivity of these 1-h immunoassay procedures for the determination of human prolactin was 2 ng/ml.

Animals↗

Effect of delta 9-tetrahydrocannabinol on herpes simplex virus type 2 vaginal infection in the guinea pig.

The present investigation was undertaken to determine whether delta 9-tetrahydrocannabinol (delta 9-THC) decreases host resistance to herpes simplex virus type 2 vaginal infection in the guinea pig. The guinea pig was selected as the host since it has been shown to express a spectrum of primary herpes genitalis which is similar to that in humans. Animals were administered delta 9-THC or vehicle intraperitoneally on Days 1-4, 8-11, and 15-18. Herpes simplex virus was introduced intravaginally on Day 2. Host resistance to virus infection was assessed by comparing frequency and severity of lesions, virus shedding, and animal mortalities. Virus-infected animals treated with drug at doses of 4 and 10 mg/kg exhibited significantly greater severity of genital disease during the 30-day period of study when compared to virus-inoculated vehicle controls. A direct relationship was noted between dose of delta 9-THC and cumulative mortalities on Day 14 following primary infection. These results indicate that delta 9-THC decreases host resistance to herpes simplex virus type 2 vaginal infection in the guinea pig.

Animals↗

Social and biocultural determinants of food selection.

Health professionals concerned with improving the nutritional status of populations are facing numerous problems ranging from emaciation to overnutrition. This review article addresses the complexity, the relative significance and the interaction of factors leading to the nutritional profile of specific groups and individuals. When availability of food is threatened, the programs designed to reduce the incidence of malnutrition must balance the food supply with energy resources and population needs as well as encourage economic improvement of food distribution systems. For those persons having access to an abundant food supply, social forces and technological factors may affect food patterns, resulting in unbalanced diets associated with chronic diseases. Misinterpretation of reliable scientific findings is a major cause of abnormal nutrition behaviour. Overreaction to health messages may precipitate such conditions as anorexia nervosa or nutrient toxicity. Adverse food reactions, real or imagined, lead to restrictions in food selection. Excessive austerity in food use negates the pleasure of eating, a useful mechanism in food choice ensuring food diversity.

Attitude to Health↗

Enhancement of aortic blood flow with a linear anionic macropolymer of extraordinary molecular length.

The addition of small amounts of high mol. wt polymers to a fluid moving through a pipe typically increases fluid viscosity and thereby diminishes the rate of flow. Under appropriate conditions of flow, however, the addition of linear polymers of high mol. wt--of the order of 10(5) to 10(7) daltons--may cause the flow to increase as much as 3-fold and occasionally more without altering the driving pressure. This effect, generally known to hydrodynamicists under the rubric 'Toms phenomenon' or as 'polymer drag reduction', generally occurs at high (greater than 1000) Reynolds numbers. However, flow also increases at much lower Reynolds numbers under certain conditions, including oscillatory, pulsatile, and helicoidal flows and flow through a column of sand. The Toms phenomenon has been repeatedly observed in various types of flow with both aqueous and organic solvents in combination with a wide variety of polymer additives. A marked fall in the pressure gradients of constant blood flows through pipes has also been observed upon the addition of polyacrylamide, poly(ethylene oxide), deoxyribonucleic acid, or a polysaccharide extracted from okra. It is the consensus among students of fluid mechanics that linear macromolecules suppress flow disturbances by stabilizing or laminizing the dynamic fluid structure when rod- or thread-like particles align themselves parallel to the axis of flow. Mostardi et al. demonstrated with hot film anemometry that aortic flow instabilities downstream from a temporary partial occlusion are dampened after infusion of a polymer drag-reducing agent, Separan AP-30 (Dow Chemical Co.).(ABSTRACT TRUNCATED AT 250 WORDS)

Acrylic Resins↗

McArdle's disease. Problems of anaesthetic management for Caesarean section.

A case is presented of the anaesthetic management of a patient with McArdle's disease who required Caesarean section. The pathology of the condition is considered together with the clinical features and treatment. The problems of anaesthesia in patients with this inherited disease are discussed with particular reference to the use of muscle relaxant drugs.

Adult↗

A twin study examining the genetic influence on food selection.

Taste sensitivity, preference and use of 24 foods were studied to identify the extent of genetic influence. Thirteen monozygotic (MZ) and ten dizygotic (DZ) adult, female twin-pairs of comparable family background participated in the research. Heritability was demonstrated for phenylthiocarbamide (PTC) sensitivity and was found in both preference and use for unsweetened grapefruit juice and green beans. In terms of preference and use separately, a heritability component was indicated in one-third and one-eighth of the foods respectively. Environmental adaptation and learning, unmeasured in the present study, would appear to exert a stronger influence than the genetic component on food-related behaviour.

Adult↗

Inheritance and genetic linkage of transcobalamin II.

The genetic polymorphism of the vitamin B12 transport protein transcobalamin II (TC II) was studied in the Caucasian population and in families. There are five codominent alleles of TC II which show a Mendelian mode of inheritance. No genetic linkage of TC II was found with gene loci for ADA, GLO I, Pi, HLA, AB0 and AK1. TC II like proteins could be detected on autoradiograph of PAGE in two patients with congenital homozygosity for functional TC II deficiency. These vitamin B12 binding proteins in the patients' serum were shown not to be normal R-proteins.

Adenylate Kinase↗

Inactivation of the plasminogen activator from HeLa cells by peptides of arginine chloromethyl ketone.

The binding specificities of human urinary urokinase (EC 3.4.99.26) and HeLa cell plasminogen activator were studied using peptidyl chloromethyl ketone inhibitors. A 125I-labeled fibrin assay has been developed to yield kinetic information. Reagents of the sequence X-Gly-ArgCH2Cl were the most effective. The susceptibility of the HeLa cell plasminogen activator differed from that of urokinase in several respects indicating the utility of this type of inhibitor in distinguishing between proteases of this specificity.

Amino Acid Chloromethyl Ketones↗