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Biomedical subjects

P Binder

Publications and source records attributed to P Binder.

At least 37 records · Page 2Linked to original sources
PubMed1993

Passive local immunotherapy of experimental staphylococcal pneumonia with human intravenous immunoglobulin.

Staphylococcus aureus remains a life-threatening agent of nosocomial pneumonia in immunocompromised patients. The increasing incidence of strains exhibiting wide-spectrum resistance to antibiotics, such as methicillin-resistant S. aureus (MRSA), requires new therapeutic strategies. There is renewed interest in passive immunization with human plasma-derived immunoglobulins (IVIG) as antiinfective agents. The efficacy of IVIG was tested in an experimental model of staphylococcal pneumonia, using both an MRSA clinical isolate and reference strain Cowan III, in mice immunosuppressed with cyclophosphamide. Efficient antistaphylococcal activities were obtained with IVIG administered intravenously or intranasally. IVIG saturated with protein A or its F(ab')2 fragments were as efficient as intact IVIG, suggesting that protection did not require opsonization through IgG Fc-phagocyte Fc gamma-receptor interactions. Thus, topical administration of IVIG may replace a local antibody response to S. aureus in an immunocompromised host and may be useful in prophylaxis and treatment of nosocomial S. aureus pneumonia.

Animals↗
PubMed1991

[Trichophagia treated successfully by intravenous iron injections].

The case of a trichophagic child is presented. Oral and intramuscular iron therapy brought only temporary effect at the age of 3. Seven years later the 10-year-old highly intelligent girl ate again hair. She was not anaemic and her serum iron level was not low. Intravenous iron injections applied again ceased immediately the psychic aberration that did not return even after 4 years. The authors are of the opinion that in cases of such psychic anomalies the iron content of the iron-rich cerebral ganglia is lower than normal and the disturbance can be ceased by the administration of iron.

Administration, Oral↗
PubMed1991

[Iron therapy in mental aberrations (pica) in childhood].

Besides of four pica cases (eating of dirt, paper, cotton, oakum, sand, lime, chalk) the authors report on a girl with trichophagia. The treatment of choice in pica consists in a sufficient supply of iron. The authors suggest that the intravenously administered iron acts on some centers of the central nervous system.

Anemia, Hypochromic↗
PubMed1990

Detection of staphylococcal enterotoxin B. A comparative study of ELISA and ELIFA systems.

The enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunofiltration assay (ELIFA) sandwich methods have been compared for their ability to detect staphylococcal enterotoxin B (SEB) in biological samples. Using the avidin-biotin amplification system in the ELISA, high sensitivity was observed and staphylococcal protein A interference was completely prevented. In contrast, ELIFA was less sensitive than ELISA, but very easy to perform, fast and simple to read routinely.

Animals↗
PubMed1990

Action of staphylococcal exfoliative toxins on epidermal cell cultures and organotypic skin.

In the staphylococcal scalded skin syndrome, spontaneous intraepithelial cleavages are due to the exfoliative toxins A or B (ETA or ETB). Until now, these toxins have been studied either on epidermis or on organotypic skin cultures. In the present study, we compare the effects of these toxins on human keratinocyte cell cultures to those on human and mouse organotypic skin cultures. With concentrations of ETA or ETB of 1 mg/ml for 3 hours, spontaneous intraepithelial cleavages were noted in both cell and organotypic cultures. Keratinocyte cell cultures were as sensitive as organotypic skin cultures to these toxins. Since keratohyaline granules may represent a possible binding site for ETA or ETB, we tried to correlate the expression of keratohyaline granules with the appearance of intraepithelial clefts due to the toxins. However, when cultured in liquid medium, epithelia were not differentiated enough to allow the detection of the binding site of ETA-ETB.

Animals↗
PubMed1988

Studies on the hexose region of the lipopolysaccharide from a low virulence strain of Salmonella typhimurium.

The structure of the hexose region of the lipopolysaccharide from M206 strain, a mutant of Salmonella typhimurium having reduced virulence, was partially determined. Immunological tests indicated cross-reactions of anti-(M206) antiserum with wild-type C5 and Ra mutant strains. Data obtained on chemical composition, periodate oxidation, acetolysis, methylation and analysis by gas chromatography/mass spectrometry show that M206 type lipopolysaccharide contains the common core polysaccharide of Salmonella which was substituted in position 4 of the subterminal glucose unit by a disaccharide: D-glucosyl 1----3 D-galactose. This substitution is probably related to the slight virulence of M206 strain.

Acetates↗
PubMed1987

Effect of culture medium on morphology and virulence of Legionella pneumophila serogroup 1.

In a preliminary study, the preparation of a modified charcoal yeast extract by predialysis of yeast extract (CDYE) allowed us to obtain short non filamentous forms of Legionella pneumophila ser 1 (Philadelphia) found to be more virulent in the chick embryo than the long forms grown on conventional media. We confirmed these findings in guinea pigs inoculated by either intraperitoneal injection or aerosol inhalation. LD50s were calculated using the method of Reed and Muench. Survival curves were established using Liddell's method. If for chick embryo the most virulent organisms were those derived from yolk sac culture, organisms grown on CDYE agar were more virulent than those grown on the other media. There was a significant positive correlation between the mean length of the bacilli and the log 10 of the LD50 (r = 0.96; 0.02 less than p less than 0.05). For guinea pigs by either intraperitoneal injection or inhalation we confirmed that the bacteria cultured on CDYE were more virulent than those grown on other solid media. Thus for the guinea pig inoculated intraperitoneally, the LD50s of the CDYE and BCYE cultures were 1.4 X 10(7) and greater than 3 X 10(9) CFU, respectively. The mortality of guinea pigs inoculated by aerosol with CDYE cultures was significantly higher than that of guinea pigs infected with BCYE cultures using suspensions of 10(8) and 10(9) CFU/ml (p less than 0.01) and 10(10) CFU/ml (p less than 0.05).

Aerosols↗
PubMed1986

[Treatment with erythromycin of experimental legionellosis in guinea pigs infected by aerosol].

The infectious strain L. pneumophila serogroup 1 Philadelphia (ATCC 33152) was cultured on charcoal dialysed yeast extract agar medium (CDYE agar) which produces more virulent strains than those grown on classical agar media. The aerosol was dispersed in a depression chamber by means of a nebuliser and the density was controlled by a density probe. Male albinos Dunkin-Hartley guinea pigs weighing 250-300 g were exposed for 30 minutes to an aerosol dose of 1 LD50 (10(3) viable organisms) and 10 LD50 (10(4) viable organisms). Erythromycin lactobionate (Abbott) was administered subcutaneously 18 hours after the infection, at dosages of 270 mg/kg/day for 4 days in the animals treated with 1 LD50 and for 6 or 7 days in the animals treated with 10 LD50. The guinea pigs were observed for 9 days (weight, rectal temperature; serological and bacteriological tests (cardiac blood, lungs, spleen) and erythromycin assays (serum, lungs) were performed and compared in the treated animals, the non-treated infected control animals and the control animals which only received erythromycin. The percentage survival in the treated guinea pigs after inhalation of 1 LD50 and 10 LD50 (2 tests) were 100%, 75% and 87.5% respectively. Three weeks after treatment, the survivors had antibody titres from 32 to 1,024; the bacteriological cultures and erythromycin assays were negative. In this study, an improvement in the treatment of experimental Legionnaires' disease was observed in comparison with previous experiments. The increased dosage and duration and the early initiation of treatment resulted in survival rates of 75%.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1985

Immunomodulation in offspring mice after neonatal immunostimulation of mothers or newborn mice.

Mothers of offspring Balb/c mice were stimulated after birth by two substances, a bacterial lysate (LAB) and a chemical, diethyldithiocarbamate (DETC). Anti-sheep red blood cell (SRBC) antibodies were studied after immunization of stimulated mothers or offspring. An increase of anti-SRBC was observed in LAB-stimulated mothers, but these antibodies were decreased in their offspring before weaning. Sometimes, these antibodies were increased in LAB-stimulated newborn mice. DETC stimulation of mothers induced an elevation of antibody response in mothers and newborns. The same results were obtained in previous investigations where the pregnant mother was stimulated with the same agents.

Adjuvants, Immunologic↗
PubMed1984

Effect of microwave irradiation (2450 MHz) on murine cytotoxic lymphocyte and natural killer (NK) cells.

Male Balb/c mice were exposed in an anechoic chamber to 2450 MHz CW microwaves at 5, 10, 15, and 20 mW/cm2, 4 hours daily for 4 days, under controlled environmental conditions. T-cell cytotoxicity and natural killer (NK) cell activity were compared in exposed and sham animals. A significant decrease (p less than 0.001) of cell-mediated cytotoxicity was observed only for a power density of 20 mW/cm2. A significant increase (p less than 0.01) in NK activity was demonstrated following exposures at 15 mW/cm2.

Animals↗