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Biomedical subjects

P Acién

Publications and source records attributed to P Acién.

At least 19 recordsLinked to original sources
PubMed2006

Aromatase expression in endometriotic tissues and cell cultures of patients with endometriosis.

Cytochrome P-450 aromatase is responsible for catalysing the conversion of androstendione into estrone, so its expression in endometriotic tissue could contribute to the development of endometriosis. The aims of this study were, on the one hand, to determine the presence of aromatase in eutopic and ectopic endometrium, healthy peritoneum, myometrium and leiomyomas from patients with (n = 61) and without endometriosis (n = 12) and, on the other hand, to determine the effect of peritoneal fluid (PF), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNFalpha) on aromatase activity from endometriotic stromal cells and subcutaneous adipocytes. After immunohistochemical analysis, aromatase expression was detected in the endometriotic tissue of 61% of patients, whereas the rest of the tissues, as well as those from disease-free women, were negative. Cell cultures were made to determine aromatase activity in endometriotic stromal cells and adipocytes. The addition of PF, TNFalpha and especially IL-6 (P < 0.05) stimulated the basal enzymatic activity observed in both cell types. Our findings confirm the presence of aromatase in endometriosis and probably the existence of a local estrogen production that may be stimulated by some factors such as cytokines present in the PF of these patients. Therefore, the use of aromatase inhibitors combined with immunomodulator agents could be a novel approach to be investigated in future clinical trials.

Adipocytes↗
PubMed2006

Experimental contributions to the study of the embryology of the vagina.

BACKGROUND: Acién's hypothesis, deduced from patients with malformations of the female genital tract, especially those with renal agenesis and ipsilateral blind hemivagina, affirms the embryology of the human vagina as deriving from the Wolffian ducts and the Müllerian tubercle and could explain the embryological origin of all the female genital malformations reported. In this study, we investigated the hypothesis in rats. METHODS: Twenty-five pregnant rats were used to analyse female embryos (64) from day 15 (stage indifferent) to day 20 postcoitum (vagina completely formed). We performed transverse and longitudinal sections of embryos, haematoxylin-eosin tinction and immunohistochemical staining using markers specific to Wolffian derivatives. We also analysed the presence of these markers in the vagina of four adult rats. RESULTS: The Müller ducts converge until they fuse into one tube, but caudally they diverge and finally they fuse with the 'urogenital sinus bulbs' that are actually the distal portion of the Wolffian ducts according to the immunohistochemical marking with GZ1 and GZ2. The Müllerian tubercle is observed between those elements. Then, the immunohistochemical staining can be seen all along the completely formed vagina, which is also observed in the vagina of the adult rat. CONCLUSION: We prove the participation of Müller tubercle and Wolffian ducts in the formation of the vagina in rats, so we confirm experimentally Acién's hypothesis about the human vagina embryology.

Animals↗
PubMed1999

Intraperitoneal and subcutaneous treatment of experimental endometriosis with recombinant human interferon-alpha-2b in a murine model.

OBJECTIVE: To determine the effect of human interferon (IFN)-alpha-2b on experimental endometriosis in rats. DESIGN: Experimental, controlled, double-blind randomized study. SETTING: Experimental surgery laboratory in a university department. ANIMAL(S): Ninety-six Wistar rats with endometriosis, induced by transplanting four endometrial fragments into the peritoneal cavity. INTERVENTION(S): One third of the animals served as controls (group A). The others were randomly divided into two groups and were given IFN-alpha-2b either as a single intraperitoneal dose (group B) or as three SC doses (on alternate days) (group C). MAIN OUTCOME MEASURE(S): Laparotomy was performed to measure the size of each implant at various times (days 0, 6, 12, 20, and 120 after treatment). RESULT(S): There were no differences among the groups in the size of the average implants before IFN was administered (17.3+/-6.7, 19.7+/-7.8, and 18.1+/-9.2 mm for groups A, B, and C, respectively). These values were significantly smaller after treatment in group B (14.9+/-8.0 mm) and group C (14.0+/-9.5 mm) than in the control group (17.6+/-7.5 mm) (P<.05). Intraperitoneal IFN produced an initial maximum decrease in the size of the implants (40% reduction on day 6), which diminished until day 20 (20%) and then was maintained on a plateau until day 120 (25%). By contrast, group C showed an initial minimal reduction (13% at day 6), which increased up to day 20 (19%), after which a plateau was reached (23% at day 120). Thus, the effects in both treatment groups were similar in the long term. CONCLUSION(S): Two short regimens of human IFN-alpha-2b reduced the size of experimental endometriosis in rats.

Animals↗
PubMed1999

Insulin, androgens, and obesity in women with and without polycystic ovary syndrome: a heterogeneous group of disorders.

OBJECTIVE: To analyze the correlations among insulin, androgens, body mass index (BMI), and other related metabolic anomalies in women with and without polycystic ovary syndrome (PCOS). DESIGN: Retrospective study of normal and obese women with and without PCOS. SETTING: Gynecologic endocrinology units of Elche, San Juan, and Alicante Hospitals and Hormone Laboratory at Alicante University Hospital ("Miguel Hernández" University). PATIENT(S): A total of 212 women were studied: 137 with PCOS and 75 without PCOS. INTERVENTION(S): BMI, gonadotropins, insulin, androgens (T, androstenedione, DHEAS), 17alpha-hydroxyprogesterone, sex hormone-binding globulin, and triglycerides were studied. Glycemia and insulin response to the tolerance test (GTT) with a 100-g oral glucose load were also assessed in 103 women. RESULT(S): A good correlation between insulin and BMI was found in normal and obese women without hormonal dysfunction and in patients with or without PCOS. Good correlations, although lower, between insulin and T, and BMI, insulin, and T with triglycerides were also found in patients with PCOS. These patients fell into clearly distinct categories: with or without insulin resistance and with or without obesity, but slim women with PCOS had insulin and metabolic variables similar to those without PCOS, and most obese women with PCOS were insulin-resistant and more hyperandrogenic and hypertriglyceridemic. CONCLUSION(S): Insulin, androgens, and BMI are related in women both with PCOS and without PCOS, especially in obese ones. Insulin and metabolic indices are similar in lean women with PCOS and those without PCOS, but obese women with PCOS are more insulin-resistant, hyperandrogenic, and hypertriglyceridemic. Three types of disorders can be distinguished: simple nonhyperandrogenic obesity, typical nonhyperinsulinemic PCOS, and insulin-resistant PCOS.

17-alpha-Hydroxyprogesterone↗
PubMed1998

Benign intracardiac teratoma detected prenatally. Case report and review of the literature.

Primary cardiac tumors are rare and, until recently, were mostly incidental postmortem findings. Nowadays, due to the widespread use of prenatal ultrasound scans, we are able to diagnose them in utero. We present a case of an intracardiac teratoma diagnosed at 38 weeks, menstrual age. Previous scans had been normal. Labor was induced, and a female infant with an Apgar score of 9 and 4, at 1 and 5 min, was delivered. Her condition worsened rapidly. She died 16 h after birth. Necropsy was performed, and a cystic, mature teratoma of 4 cm was found in the interventricular septum, growing into the right ventricle. No other anomalies were found. This probably represents the first case of an intracardiac, benign teratoma diagnosed prenatally.

Fatal Outcome↗
PubMed1997

Clinical and hormonal effects of the combination gonadotrophin-releasing hormone agonist plus oral contraceptive pills containing ethinyl-oestradiol (EE) and cyproterone acetate (CPA) versus the EE-CPA pill alone on polycystic ovarian disease-related hyperandrogenisms.

The aim of this study was to compare the clinical and hormonal effects of the combination of a long-acting gonadotrophin-releasing hormone analogue (GnRH-a) plus an oral contraceptive (OC) pill containing ethinyl-oestradiol (EE) and cyproterone acetate (CPA) versus the EE-CPA pill alone in patients with polycystic ovarian disease (PCOD) and related hyperandrogenisms, in order to evaluate whether the addition of GnRH-a has any advantage. A total of 12 PCOD patients were treated with the EE-CPA pill alone for 10 consecutive cycles according to an OC standard regimen. A further 12 patients were treated with GnRH-a, one i.m. injection every 28 days for a total of eight injections, combined with the EE-CPA pill for 10 consecutive cycles. The latter was thus prolonged for two cycles more than GnRH-a. Clinical evaluations (symptoms, weight, Ferriman-Gallwey score) and hormonal and biochemical analyses were assessed before, during (at 3 or 6 months) and after treatment, either when spontaneous cycles had resumed or after 3 months of amenorrhoea. There was a significant improvement in hirsutism, and a strong reduction in gonadotrophin, oestradiol, testosterone, androstenedione and 17-OH-progesterone concentrations in both treatment groups but with no significant differences between them, except in the gonadotrophin concentrations. Cortisol and triglyceride concentrations increased during treatment in both groups. The Ferriman-Gallwey score remained significantly decreased in both groups after treatment, as did androstenedione in the GnRH-a plus EE-CPA pill group, but there were no significant differences between the two groups. No changes were observed in prolactin, dehydroepiandrosterone sulphate (DHEA-S), insulin, glycaemia and cholesterol concentrations. However, when only the obese and more hirsute patients were considered, significant differences between the two groups were found during treatment in the Ferriman-Gallwey score and the gonadotrophin and DHEA-S concentrations (which increased during treatment in obese patients with the pill alone), and after treatment in the Ferriman-Gallwey score and the concentration of 17-OH-progesterone in the more hirsute patients, with the GnRH-a plus pill group having better results. In conclusion, a cyclic prolonged treatment with OC EE-CPA pills is not improved in most PCOD patients by the addition of GnRH-a, and is complicated and expensive. However, the addition of a long-acting GnRH-a may be recommended in obese and severely hirsute patients.

Adolescent↗
PubMed1996

A comparative study on breech deliveries attended in 1992: hospital centers from Latin America versus Spain and Portugal.

OBJECTIVES: The first aim of this study was to compare the incidence and management of breech deliveries, as well as perinatal mortality rates, in centers from Latin America versus Spain and Portugal in Europe. A second aim of this study was to analyse the correlation between the rates of c-sections and perinatal mortality. METHODS: Data from 287 313 deliveries, 8,764 of which were singleton breech deliveries attended in 1992 in 30 centers from Latin America and 44 centers from Spain and Portugal, were provided by the doctors in charge of the obstetric services and analysed using a computer statistical package (R-sigma, Horus Hardware). RESULTS: The incidence of breech deliveries was lower in centers from Latin America than in Spain and Portugal, 2.6% vs. 3.7%, respectively, but the percentage of preterm breech deliveries was higher in the former (23% vs. 17%; P < 0.001). In the total number of deliveries, c-sections were performed more frequently in Latin America, but the perinatal mortality rates were triple those in Spain and Portugal. In breech presentations from both geographical areas nearly 70% of c-sections were performed. However, the perinatal mortality rates in breech presentations were more than twice those of the total number of deliveries in Latin America, and more than three times in Spain and Portugal. There was no correlation between percentage of c-sections and perinatal mortality rates in the different centers in Spain and Portugal, but this correlation was significantly negative in both all and breech deliveries from centers in Latin America. Breech deliveries were more frequently monitored and attended by a specialist in Spain and Portugal. CONCLUSIONS: Preterm deliveries, c-sections and perinatal mortality were more frequent, and the correlation between these two latter rates was significantly negative for both all and breech deliveries in Latin America. As long as basic health care is still an issue in certain countries, it seems that a high rate of c-sections could prevent or reduce perinatal mortality. Yet, in countries with better health care standards, there is probably no need to perform c-section in most breech presentations.

Breech Presentation↗
PubMed1996

Individual and combined effects of triptoreline and gestrinone on experimental endometriosis in rats.

OBJECTIVES: To evaluate the effects of triptoreline, gestrinone, and both, on experimental endometriosis in rats. STUDY DESIGN: Experimental endometriosis was surgically induced in 225 Wistar rats. Of these, 202 rats showed at least one grown implant, 22 of which composed the control group, while 180 were treated with triptoreline, gestrinone, or both, for 28 days. The implants were evaluated again after 25 days. RESULTS: There were no changes in size in the control group. About 73% of the implants treated with triptoreline showed a high reduction (> 50%), vs. 51% with gestrinone (P < 0.0005) and 65% with both (P < 0.005). Triptoreline caused macroscopic resolution in 40% of the implants vs. 31% for gestrinone (not significant) and 26% for both substances (P < 0.05). In the triptoreline group, the mean size of the implants decreased by 65% between the 25th and 28th days, 58% between the 29th and the 35th, and 39% after the 36th day. This reduction was 51%, 36%, and 33%, respectively, in gestrinone group. CONCLUSIONS: Triptoreline was more effective than gestrinone, but perhaps not in the long run. Their association did not improve the results.

Animals↗
PubMed1996

Abdominal myomectomy: results of a simple operative technique.

OBJECTIVES: To report a simple and safe procedure of abdominal myomectomy and our results performing this technique. DESIGN: The operative technique comprises the incision on the most prominent part of the myoma, the use of a hooked clamp to hold the tumor, and a surgical knife to peel it, without removing the apparent excess of myometrium or serosa. SETTING: University Medical Centers and private practice. PATIENTS: Eighty patients, 9 of whom were operated between 10 and 26 weeks of pregnancy, 3 during cesarean section, and 22 others who had infertility. RESULTS: Myomectomy was performed successfully in all patients for whom it was scheduled. Eight of nine pregnant patients had successful deliveries at term. The cumulative 10-year reoccurrence and reoperation rates of life-table analysis were 38% and 18%, respectively. The cumulative conception rates were 100% for otherwise unexplained infertility at 2 years, and 63% and 79% at 5 years for all infertile and all patients attempting conception after myomectomy, respectively. An age > 30 years, infertility > 3 years, and multiple fibroids negatively affected these rates, whereas the use of an absorbable adhesion barrier (Interceed; Johnson & Johnson AB, Somerville, NJ) had a positive effect. CONCLUSIONS: This procedure is an appropriate alternative for most women who want to preserve or enhance fertility potential, and if necessary, for pregnant women.

Adolescent↗
PubMed1995

Breech presentation in Spain, 1992: a collaborative study.

OBJECTIVES: The goals of the present work were to study the incidence of breech deliveries in Spain, the causes for breech presentation and its management and the incidence of cesarean sections and its correlation with the perinatal mortality rates among different centers. STUDY DESIGN: Data from 102,038 deliveries, 3829 of which were singleton breech deliveries attended in 1992, were obtained from 40 hospital centers located in different autonomic communities of Spain and analyzed using a computer statistical package (R-sigma, Horus Hardware). RESULTS: The incidence of breech deliveries was 3.8%, although this proportion varied between different centers and regions. Up to 18% of the cases occurred in preterm. The most frequent causes were prematurity and uterine malformations. In its management, an increase in the tendency to perform elective cesarean section was noticed. The incidence of cesarean sections reached 14.9% of the total and 67.9% in breech deliveries. Perinatal mortality rate in breech deliveries was more than four times that of all deliveries, according to national standards, and more than three times according to international standards (47.8 versus 11.8 x 1000, and 24.7 versus 8 x 1000, respectively). The intrapartum and neonatal mortality in breech deliveries was 13 x 1000. No correlation between incidence of cesarean sections and perinatal mortality rates was found. Primiparity, deflexed head, macrosomia and prematurity were the most decisive parameters to indicate a cesarean section. Neither the external cephalic version nor the total breech extraction were common procedures. CONCLUSIONS: The incidence of breech deliveries is variable between different centers (1.7%-5.1%) and Spanish regions (3.3%-4.3%), with a mean of 3.8% in overall and 11.4% in preterm deliveries. There is a tendency to perform cesarean sections, especially in primiparae. There is no correlation between the percentage of cesarean sections in each center and the perinatal mortality rate. External cephalic version and total breech extraction were uncommon procedures.

Breech Presentation↗
PubMed1993

Reproductive performance of women with uterine malformations.

The fertility problems of 176 patients with uterine malformations [arcuate (n = 40), bicornuate (n = 49), bicornis-bicollis (n = 17), didelphys (n = 15), unicornuate (n = 24), subseptus (n = 14) and septate uterus (n = 17)] and of 28 women with other genital and/or urinary anomalies but with a normal uterus were studied. Ten patients with a uterine anomaly experienced infertility without other causes (6%). 142 women with uterine malformations and 26 with a normal uterus achieved pregnancy, the total number of pregnancies to date being 383 and 47 in these groups respectively. The outcome of the first pregnancy from women with uterine malformations was similar to that of all pregnancies though less significant. Only 53% of pregnancies in women with uterine malformations ended with a child surviving > 7 days, compared to 89% in women with a normal uterus. The poorest viability results were found in the bicornuate (40%) (47% of pregnancies in this group ended in early abortions), arcuate (45%) and septate uterus groups (59%). The rates of children surviving > 7 days were around 70% in the bicornis-bicollis, didelphys, unicornuate and subseptus uterus groups. Metroplasty in four cases corrected the infertility or repeated abortions in three patients. Cerclages (21) in 14 women increased the live birth rate from 21 to 62%. These results confirm earlier reports that patients with uterine malformations have higher rates of reproductive loss, pre-term delivery, breech presentation and complications that increase obstetric intervention and perinatal mortality. Moreover in our study, pregnancy outcome was poorer in the bicornuate and arcuate uterus groups than in the septate group.(ABSTRACT TRUNCATED AT 250 WORDS)

Female↗
PubMed1992

Experimentally induced endometriosis in rats: effect on fertility and the effects of pregnancy and lactation on the ectopic endometrial tissue.

OBJECTIVE: To investigate the repercussion of experimentally induced endometriosis on the fertility of Wistar rats and the effects of pregnancy and lactation on endometrial ectopic implants. DESIGN: Eight-five animals were used for the experiment. Experimental endometriosis was induced in 44 and the remaining 41 were sham-operated (control group). After evaluating the growth of the implants in a second laparotomy, all the animals were mated until finding sperm in vaginal smears. In third and fourth laparotomies during pregnancy and lactation, pregnancy rate was determined, and the implants were re-evaluated, being resected for histological study. Serum estradiol (E2) and progesterone (P) levels were also measured. RESULTS: The pregnancy rate in animals with endometriosis was 65.7% versus 100% in control group. No statistical differences in days of gestation and number of pups were found between the two groups. Ectopic implants underwent a significant regression during lactation period. Serum E2 and P levels and histological features sustained these findings. CONCLUSION: Experimentally induced endometriosis diminishes pregnancy rate in rats. It is not pregnancy, but the anestrus condition created by lactation that exerts a beneficial effect on experimentally induced endometriosis in rats.

Animals↗
PubMed1992

Embryological observations on the female genital tract.

The embryology of the genital tract and urinary system is described, and the hypothesis is advanced that the vagina is an organ embryologically derived from the mesonephric or Wolffian ducts in addition to the Müllerian tubercle. This is based on experimental studies and case reports in the literature and our own cases of genital malformations, especially in patients with unilateral renal agenesis and an ipsilateral blind vagina. The importance of the mesonephric ducts as guides or 'inductor' elements for adequate Müllerian development is emphasized. A new embryological classification of female genital malformations is proposed, based on these embryological concepts.

Female↗
PubMed1991

Renal agenesis in association with malformation of the female genital tract.

Four cases of renal agenesis with an ipsilateral blind vagina and two cases with agenesis of all organs derived from one urogenital ridge are presented. These cases confirm the association of renal agenesis with these genital malformations and support the hypothesis that the embryologic development of the vagina is derived from the fused mesonephric ducts.

Adolescent↗
PubMed1990

Perinatal morbidity and mortality in pregnancy hypertensive disorders: prognostic value of the clinical and laboratory findings.

In 521 pregnancies complicated by hypertensive disorders (PHD) and in 200 control cases, we studied the incidence of intrauterine growth retardation (IUGR), depression in the newborns, general morbidity of live newborns requiring admission and perinatal mortality. We also analyzed the relationship between these conditions and the type and severity of hypertension, gestational age, presence of symptoms of the classic EPH triad and of abnormal uric acid values, hemoconcentration, and low urinary estriol values. Perinatal mortality (especially antepartum) was significantly increased in severe pre-eclampsia, chronic hypertension and chronic hypertension with superimposed pregnancy-induced hypertension (PIH); in all the cases with PHD it was three times higher than that of the control group (59% versus 20% and five times higher than the global perinatal mortality of the 25,763 deliveries attended during the same period (12% General morbidity reached 44% in severe pre-eclampsia and 75% in antepartum eclampsia. But the preterminal deliveries were also more frequent in PHD, especially in severe pre-eclampsia-eclampsia. Nevertheless, the perinatal morbidity and mortality in general increased when proteinuria and edema plus proteinuria were associated with hypertension, and the incidence was significantly higher when proteinuria surpassed 100 mg/dl. Morbimortality also increased in the presence of hemoconcentration, hyperuricemia, and low estrioluria.

Adult↗