Intranasal administration of two doses of the inactivated influenza vaccine prepared in the "Stefan S. Nicolau" Institute of Virology was followed by rises in the level of neutralizing secretory influenza antibodies in 82% of the cases. The concomitant study of secretory antibody, IgA and total protein levels, as well as of the serum HAI influenza antibodies demonstrated that their evolution was parallel only in 23% of the vaccinees. The percentage of secretory antibody conversion was similar to the rate of protection conferred by the vaccine.

Administration, Intranasal↗

PubMedDate not supplied

[Studies of the influenza epidemic of January-March, 1974].

In an epidemiologic, clinical and viral study of several influenza foci in some urban districts of Romania during January-March 1974, 23 influenza virus B Hong-Kong 8/73 strains were isolated. The dynamics of HAI serum antibodies confirmed the viral diagnosis. The epidemic ran a slow course, affecting especially the 14-25 years age group and had an evident benign clinical aspect.

Adolescent↗

PubMedDate not supplied

[Study of the multiplication in cell cultures of two strains of para-influenza virus type 3. III. Multiplication in BHK 21 cells].

Study was conducted on the multiplication of two strains (C243 and D) of parainfluenza virus type 3 in BHK 21 cells. Multiplication curve of the virus was established and immunohistochemical aspects of the process were investigated. Chronological study of successive steps of the formation and development of viral components allowed to see that the virus multiplication rate is low in this cell system. The parainfluenza antigen became detectable by immunofluorescence in the infected cell perinuclear region after a relatively long eclipse period (18 h) and synthetized virus has few RNA and induced no inclusion information in the cytoplasm or the nucleus. However, an important nuclear participation was noted: 72 h after inoculation, nuclear fluorescence was observed, as well as a nuclear DNA rising and frequent aberrant mitoses. Comparison between the two investigated strains led to the observation that the autochthonous D strain induced more frequent aberrant mitoses and more important cell destruction than the C243 one. Differences were also noted as regards the infecting and hemagglutinating titers.

Animals↗

PubMedDate not supplied

Persistence of viruses in the nasopharynx of apparently healthy children aged 0-5 years. Results of investigations performed in 1982-83.

Virus isolation attempts were made with nasopharyngeal secretions from 400 apparently healthy 0-5-year-old children of a semi-closed community. Repeated virus isolations were achieved in 25 cases subjected to periodic investigations in 1982-83. One-two reisolation(s), 1-5 months apart, of the initially detected viral agents could be obtained in 15 out of the 25 children. The following viruses were reisolated (in decreasing order of frequency): adenoviruses, respiratory syncytial virus, Coxsackie B1 virus, herpes virus type 1, parainfluenza virus type 3. The problems raised by the carriage of respiratory viruses in the nasopharynx of apparently healthy children are discussed.

Adenoviruses, Human↗

PubMedDate not supplied

Effect of the oral administration of NIVGRIP inactivated influenza vaccine on virus carriage in the nasopharynx of children aged 0-5 years.

The NIVGRIP inactivated influenza vaccine was administered by oral route to 11 children of a semi-closed pre-school community, found to carry viruses in their nasopharynx. Periodic virological investigations demonstrated that after vaccination virus carriage disappeared completely in 7 children; in the remaining 4 cases there was a considerable decrease (from 14 to 6) in the number of virus strains isolated. The virus isolates obtained after vaccination were represented exclusively by enteroviruses (Coxsackie B1 and B3, poliovirus type 1), in contrast with the wider range of viral agents (adeno-, parainfluenza, herpes, Coxsackie, and polioviruses) detected prior to vaccine administration.

Administration, Oral↗

PubMedDate not supplied

[Effect of immunization with an inactivated influenza vaccine, NIVGRIP, on the viral population and clinical course of patients with chronic non-bacterial pharyngitis].

Study was conducted on 20 subjects with non bacterial chronic rhinopharyngitis. Forty-one virus strains, mainly adenoviruses, type 3 parainfluenza virus, influenza and type 1 herpes viruses, as well as enteroviruses were isolated from samples collected at regular intervals. After the NIVGRIP (R) vaccination, the number of subjects with viruses present in the pharynx decreased by 75% (from 20 to 5 positive patients) and that of isolates from 41 to 6 (80% positive samples before vaccination against 10% after immunization).

Adenoviruses, Human↗

PubMedDate not supplied

Research on respiratory virus diseases performed in the "Stefan S. Nicolau" Institute of Virology.

The studies on respiratory virus diseases performed for 30 years in the "Stefan S. Nicolau" Institute of Virology approached various aspects of the ecology of inlfuenza and parainfluenza viruses, adenoviruses and other respiratory viruses, including numerous experimental, fundamental, clinical and morphopathological investigations. Special attention was paid to specific influenza prophylaxis and to the preparation of an inactivated influenza vaccine applicable by nasal and oral route.

Animals↗

PubMedDate not supplied

Efficiency of the NIVGRIP inactivated influenza vaccine applied by oral route: evaluation in a community of pre-school children in the period 1981-84.

The NIVGRIP inactivated influenza vaccine prepared in the "Stefan S. Nicolau" Institute of Virology was administered by oral route to 3-6-year-old children of a preschool community in October 1981, 1982 and 1983. There were no cases of acute respiratory tract infection (ARTI) in the community in the winter seasons of 1981-82, 1982-83 and 1983-84, with the exception of 3 ARTI cases recorded in February 1982 in unvaccinated children. In a similar community where no vaccination had been performed morbidity by ARTI was of 20% in 1982-83 and 12.6% in 1983-84. The efficiency and the advantages of oral influenza vaccination are outlined.

Administration, Oral↗

PubMedDate not supplied

Dynamics of neutralizing secretory influenza antibodies after nasal administration of the NIVGRIP trivalent influenza vaccine.

The trivalent inactivated NIVGRIP influenza vaccine was administered by nasal route to 26 young adults; a matched control group was represented by 19 subjects who received a placebo. Vaccination was followed by a higher proportion of rises in the level of neutralizing secretory antibodies (70-77%) than in that of serum HAI antibodies (19-42%) to the three influenza strains contained in the vaccine. Simultaneous rises in secretory antibody titers to three and two influenza antigens occurred in 50% and 23% of the vaccinees, respectively. The vaccine conferred a protection rate of 75%, as estimated by a 7-month clinical follow-up.

Administration, Intranasal↗

PubMedDate not supplied

Viral and rickettsial etiology of an outbreak of acute respiratory tract infection.

An outbreak of 111 cases of acute respiratory tract infection was recorded in a community of the town "T" in April-May 1984. The clinical picture was severer than usual; 28% of the cases had to be hospitalized, average absenteeism being as high as 26 days per case. Serological investigations demonstrated the previous circulation of influenza virus B/Singapore/222/79 and the simultaneous circulation during the outbreak of influenza virus A/England/333/80 (H1N1) and of Rickettsia burneti (as also ascertained by isolation in the chick embryo of the former and by visualization by immunofluorescence in exfoliated cells of the latter pathogen). The association of the two etiological agents appears to account for the severe and protracted course of the disease.

Acute Disease↗

PubMedDate not supplied

Susceptibility of irradiated mice to inoculation with two influenza virus strains.

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Animals↗

PubMedDate not supplied

Isolation of parainfluenza virus type 3 from an infant with meningoencephalitis.

A parainfluenza virus type 3 strain was isolated from the cerebrospinal fluid of an infant with a clinical diagnosis of meningoencephalitis. Specific HAI antibodies to parainfluenza virus type 3, ranging in titer from 1/80 to 1/160 could be detected in the infant's serum. A 2-fold rise in the level of complement fixing serum antibodies-from 1/16 at the first to 1/32 at the second collection-was recorded.

Antibodies, Viral↗

PubMedDate not supplied

Extrarespiratory localizations of parainfluenza viruses.

Two hemagglutinating and hemadsorbant agents-serologically identified as parainfluenza viruses type 3-were isolated from the cerebrospinal fluid of a 10-month-old infant with meningoencephalitis and from the urethral secretion of a male patient with nonbacterial urethritis. Parainfluenza virus antigens types 1, 2, and 3 were detected by indirect immunofluorescence reactions in cells exfoliated in the vagina of women with genital neoplasia or common gynecopathies.

Adult↗