Biomedical subjects
Publications and source records attributed to O Tanaka.
From pericarps of Rosa davurica (Rosaceae), a traditional Chinese medicine, eight known tetracyclic triterpene acids, three known flavonoids, ethyl beta-fructopyranoside and methyl 3-O-beta-glucopyranosyl-gallate were isolated.
From leaves of Kalopanax pictum var. maximowiczii, a Korean medicinal plant, six known saponins of hederagenin were isolated. One of the monodesmosides was identified as sapindoside A, previously isolated from Sapindus spp. Another monodesmoside and four bisdesmosides were proved to be identical with saponins-K3, -K10 and -K12 and Kizuta saponins-K8 and -K11, respectively, all of which have been isolated from Hedera rhombea. It was observed that the water solubilities of these monodesmosides were increased in the presence of the co-occurring bisdesmosides. The relationship between structure and solubilizing effect is reported.
From rhizomes of Panax japonicus (Araliaceae), two polysaccharides named tochibanan-A and -B, which show reticuloendothelial-potentiating activity in the carbon clearance test in mice, were isolated. The structure of tochibanan-A (molecular mass: 23,000) was elucidated as a linear beta-1,4-D-galactan. Tochibanan-B (molecular mass: 40,000) consists of D-galactose (87.1%), L-arabinose, D-glucose and D-galacturonic acid and has a beta-D-(1----4)-linked galactopyranosyl backbone possessing GalA-(1----6)-Gal, Ara-(1----5)-Ara, Gal, and Glc side chains. The structure around the branching points and the repeating unit were investigated and a possible structure of tochibanan-B is proposed.
Ten patients received augmentation of deficient mandibular alveolar ridges using particulate hydroxyapatite (HAP). Masticatory ability was evaluated preoperatively and postoperatively by a questionnaire with 35 foods listed and by the spectrophotometric method using ATP granules. The results showed that masticatory ability when wearing newly constructed complete dentures was significantly enhanced compared to that when wearing old dentures or relined dentures, and that the surgical procedure and the use of the surgical stent greatly improved masticatory ability when wearing the new dentures. The sensitivity of the mucose membrane of the augmented mandibular alveolar ridge was examined during a postoperative period of 24 months by using an electric nerve stimulator. The sensitivity of the mucose membrane decreased postoperatively, especially at the buccal site of the molar region, the so-called buccal shelf. The level of sensitivity increased periodically, and did not return to the average level for normal edentulous mucosa even 24 months after the augmentation. The patients were highly satisfied with the new dentures and the HAP augmentation judging from the questionnaire. From these points of view, HAP augmentation in edentulous patients having severely resorbed alveolar ridges has proved to be beneficial in allowing the recovery of masticatory function.
In order to investigate tongue skill, upward and downward tongue movements with two constant frequencies were observed by using an ultrasound system in young and elderly adults. Upward and downward movements in accordance with a metronome were recorded by ultrasound recorder from the Motion-mode. Movement rhythm and movement velocity of the tongue were compared in the two age groups. The main findings were: 1. The ultrasound system with 5 MHz ultrasound convex transducer and Motion-mode observation produced clear traces of the tongue surfaces in the sagital plane during upward and downward movements. 2. Upward and downward movement rhythms in the elderly were more irregular than those of young adults. 3. Velocity of tongue upward and downward movements were statistically decreased in the elderly (p less than 0.01). Velocity did not clearly change if the movement speed was different in the elderly. Results of this study revealed that oral motor behaviour in the elderly differed from that of young adults.
This work was an attempt to elucidate the diagnostic evaluation of sialography+ and histopathological examination of the salivary gland in Sjögren's syndrome. The author investigated the correlation of the sialography and histopathology features of the salivary gland in 89 confirmed cases of Sjögren's syndrome and 26 suspected cases, a total of 115 subjects and the following results were obtained. 1) In determining the histopathological severity of the salivary gland lesions from sialography, the parotid sialography was more useful than the submaxillary sialography. 2) The severity of parotid sialography correlated well that of labial gland histopathology. 3) Biopsy of the labial gland was useful for histopathological diagnosis on Sjögren's syndrome but the histopathological change occurred to a greater degree in the submaxillary gland than in the labial gland. 4) From above results, in histopathological examination of severe case of Sjögren's syndrome, biopsy of the labial gland will be sufficient, but biopsy of the submaxillary gland must be employed for studying mild cases.
The histopathological findings of the salivary glands in Sjögren's syndrome and of the thyroid gland in chronic thyroiditis have similarity in lymphocytic infiltration of the stroma and decrease or disappearance of parenchyma. In order to investigate the pathogenesis of Sjögren's syndrome, the author performed serological and histopathological examination of the thyroid gland in 89 confirmed cases of Sjögren's syndrome and 26 suspected cases, a total of 115 subjects and the following results were obtained. 1) Thyroidal microsomal and thyroglobulin antibodies were detected in 34.5% and 21.4% of 89 confirmed Sjögren's syndrome. 2) The positivity rate of the antithyroid antibodies increased in the more severe histopathological changes of the thyroid gland and thyroidal microsomal antibody, in particular, reflected the absence or presence of lymphocytic infiltration of the stroma. 3) The correlation between histopathological findings of the thyroid gland and titers of antithyroid antibodies was insignificant. 4) The correlation between histopathological grading of thyroid gland and of salivary glands was insignificant. 5) Approximately 30% of confirmed cases of Sjögren's syndrome presented chronic thyroiditis as a complication, therefore, it was suggested that both Sjögren's syndrome and chronic thyroiditis might have a common etiology.
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The reproductive organs in non-obese diabetic (NOD) mice were histopathologically studied, in order to elucidate the relationships between developmental abnormalities, such as diminished rates of implantation and viable embryos, and structural changes in the reproductive organs. NOD mice with (NOD-DM) and without (NOD-N) diabetes mellitus and ICR mice were compared. The severity of histopathological changes in the pancreas and in the liver were used as parameters which indicated the severity of diabetes itself and of the secondary metabolic disorder. NOD-DM mice exhibited uterine weight loss, accumulation of lipids in luminal and glandular epithelium, atrophies of the endometrium and myometrium and a decrease in the number of muscle cell layers. They also showed a high concentration of lipid droplets in ovarian granulosa cells, atretic follicles and atrophy and lack of lipids in ovarian stroma cells. The severity of these structural changes in the reproductive organs corresponded to those of the changes in the pancreas and the liver. The structural alterations in the ovary suggested disorder in oocyte maturation. The structural changes in the uterus appeared to be related to the decrease in the ratios of implantation and of viable embryos at post-implantation stage. The present studies suggest that the impaired structural environment together with the metabolic environment caused the abnormal development seen, for example, in the oocyte maturation, and at the implantation and post-implantation stage of diabetic mice. It also caused alterations in their hormonal environment.
The ultrastructural relations of the infiltrating mononuclear cells to the parenchymal tissues were studied in the submandibular gland of the female non-obese diabetic (NOD) mouse. In addition, the phenotype of mononuclear cells infiltrating the submandibular gland has been determined by light and electron microscopy by using monoclonal antibodies against T-cell subsets (Thy1.2, Lyt1, Lyt2). Ultrastructurally, lymphoid cells were frequently observed around and in the acini and ducts. Some of the lymphoid cells observed in the acini and ducts were irregular in shape and sometimes sent spike-like projections into acinar and ductal cells. Immunohistochemical study demonstrated that Thy1.2+ cells were predominant among the infiltrating cells, and the majority of these infiltrating T-cells were composed of Lyt1+ cells with a small proportion of Lyt2+ cells. By immunoelectron microscopy, lymphocytes carrying Thy1.2, Lyt1 or Lyt2 antigen were identified, as is demonstrated by an electron-dense reaction product on the entire cell surface, and these immunopositive cells were frequently observed around and in the acini and ducts. Some of the Thy1.2+, Lyt1+ or Lyt2+ cells observed in the acini and ducts demonstrated a close contact with acinar and ductal cells and both Lyt1+ and Lyt2+ cells sent spike-like projections into them. Occasionally, a partial degeneration of acinar cell adjacent to the invading lymphocytes was observed. These observations suggest that T-lymphocytes are involved in the direct destruction of acinar and ductal cells in the NOD mouse submandibular glands.
UNLABELLED: 1. MATERIALS: One hundred and seventy nine patients with thoracic esophageal carcinoma who underwent an intrathoracic esophagectomy combined with systematic lymphadenectomy were investigated. They were roughly classified into two groups, i.e., those who received extensive lymphadenectomy in bilateral cervical and upper mediastinal regions (A group: 78 subjects), and those who underwent ordinary limited dissection of the lymph nodes in the left cervical and upper mediastinum (B group: 101 subjects). 2. RESULTS: Cumulative 5-year survival rate of advanced cancer patients was 31.3% in A and 22.5% in B, the difference being of significance (p less than 0.05). The rate of postoperative mortality showed no difference between both groups, i.e., 3.8% in A and 4.9% in B groups. However, the incidence of postoperative pulmonary complication was 17.9% in A and 13.9% in B. Recurrent nerve palsy developed in frequencies of 39.7% and 17.8% in A and B groups respectively. 3. CONCLUSION: The degree of extended lymph node metastasis in carcinoma of the thoracic esophagus was closely correlated with its prognosis, and hence it is extremely important to perform intrathoracic esophagectomy with extensive systematic lymphadenectomy aiming at the favorable result in surgical treatment. It is also required to take preventive measures against postoperative complications.
The circulus arteriosus cerebri (circle of Willis) was examined to observe morphological variations in 50 normal human brains. Multiformed variations, as well as unruptured intracranial aneurysms, were observed. Among such variations, particular attention was paid to configurations of the two communicating arteries, anterior and posterior, respectively. Variations of the anterior communicating artery were characterized by multiplicity in their forms, on the other hand the posterior communicating arteries exhibited hypoplastic or primitive configurations. We suggest that, possible morphological variations which occur in the course of circulus arteriosus cerebri and their main branches must not be confused with congenital malformations. There has been no critical definition of the normal circle arteriosus cerebri. Differences in morphological variations between the anterior and posterior communicating arteries are discussed from the viewpoint of both embryology and the clinical findings.
The perioperative courses of 5 open heart operations in children of Jehovah's Witnesses are reviewed. The age of patients ranged from 6 to 13 years old and the body weight, from 21.5 kg to 38.5 kg. All the patients survived and are doing well now. The cardiopulmonary bypasses, primed with lactated Ringer's solution, were performed safely with the use of moderate hypothermia. Hemodilution techniques are the key for the safe exocorporeal circulation and we set the lowest limit of body weight at 20 kg. Postoperative bleeding occurred in one case and was treated by early exploration. All the cases did not receive any blood or blood products during hospitalization. Therefore we can conclude that cardiac operations over 20 kg of body weight can be safely performed without any blood or blood products. With regard to social aspects, especially in case of emergency, we should make the decision regarding transfusion under the guidance proposed by the Japanese Medical Association.
Seventy patients who underwent aortic and mitral valve replacement were reviewed. 37 patients had the mitral valve replaced with preservation of posterior mitral apparatus (AVR and modified MVR; modified group), and the others without (AVR and conventional MVR; conventional group). Hospital deaths occurred in 4 patients (12.1%) in conventional group and in only 1 patient (2.7%) in modified group. Four out of these five hospital deaths were due to low output syndrome (LOS). The other one died of unknown cause. Severe LOS, necessitating intra-aortic balloon pumping, occurred in 4 (12.1%) in conventional group and in 2 (5.4%) in modified group. In the early postoperative period modified group showed better left ventricular function than conventional group in terms of cardiac index and left ventricular stroke work index. Cardiac performance was evaluated by means of echocardiography at the time of discharge. Modified group showed better fractional shortening and shorter PEP/ET (pre-ejection period/ejection time). These findings suggest that the lower mortality of modified group is due to better left ventricular function preventing LOS in the early postoperative period, and that preserving the posterior mitral apparatus has a more beneficial effect on postoperative left ventricular function even in combined valve disease.
Six patients with tuberous sclerosis were evaluated with computed cranial tomography (CCT) and magnetic resonance imaging (MRI). The results were assessed in comparison with the clinical severity of the disease. The brain lesions were shown by MRI as low-signal areas on IR images (T1 weighted sequences) and high-signal areas on SE images (T2 weighted sequences) Three patients, who had severe psychomotor retardation (DQ less than 70) and intractable epileptic seizures following infantile spasms, had many cortical and subcortical lesions. In the other three patients, intelligence was normal or slightly retarded (DQ or IQ greater than 70) and epileptic seizures were well controlled, and small subependymal lesions were observed. Cortical lesions were rare. These results indicate that MRI can detect more precisely intracranial lesions in tuberous sclerosis.
The localization of three carbohydrate antigens, Lex, Ley, and sialylated Lex-i, which are closely related to stage-specific embryonic antigen 1, in the lung of developing human embryos was investigated using specific monoclonal antibodies. In the 38-day-old embryo, when the primitive lung bud has appeared and developed into two lung sacs, only Ley antigen was specifically positive in the proliferating cells in the terminal portion of lung bud. In the 50-53-day-old embryos, the future bronchi were actively developing from the bronchial buds. At this stage, the Ley antigen was maximally expressed and the Lex antigen appeared in the bud cells. In the lung of the 12-week-old embryo, buds for the future bronchioles were lined by simple cuboidal epithelial cells, which were strongly positive for Lex antigen, weakly positive for Ley antigen, and still completely negative for sialylated Lex-i antigen. Sialylated Lex-i antigen finally appeared in 18-week-old embryos, in the cells of the terminal buds for the future alveoli. At this stage, the Lex and Ley antigens were already beginning to disappear and were only weakly positive in cells of terminal buds. At 20 weeks, only sialylated Lex-i antigen was weakly detected in the cells in the terminal buds; after 8 months, all three antigens were essentially not detected in the respiratory cells in most of the embryos examined in this study. Formation of bronchial glands was detected at 18 weeks, where the developing gland cells were specifically positive for sialylated Lex-i antigen. Ciliation of the bronchial epithelial cells started at 12 weeks and propagated thereafter. The ciliation was accompanied by the reappearance of Ley and Lex antigen in the epithelial cells. These findings collectively indicated that the three antigens all have a physiological significance as stage-specific developmental antigens of the human lung; those antigens were specifically present in the bud cells at each important step of the morphogenesis of the human lung, such as cells in the lung buds, bronchial buds, and terminal buds for the formation of the alveolus, and cells differentiating into bronchial gland cells. The three antigens gradually disappear in the later stage of development along with the maturation process of the lung. Stage-specific embryonic antigen 1 and related antigens are known to be associated with various human cancers, including lung cancers. We suggest that the expression of these antigens in the lung cancer cells is the result of the retrodifferentiation of the cancer cells to the stages of immature embryonic lung cells.
The average dose of vecuronium required in children continuous infusion to attain a steady state block of 90% was determined. The electromyographic (EMG) response and mechanical response to supramaximal stimulation of the ulnar never recorded simultaneously, were significantly correlated in four children. The steady-state infusion rate requirement of vecuronium was 1.4 +/- 0.03 micro g/kg/min during 2% enflurane anesthesia and 3.1 +/- 0.03 micro g/kg/min during 1% halothane anesthesia. The spontaneous recovery time to 25% of the control by EMG during halothane and enflurane anesthesia was 12.6 +/- 1.1 and 10.3 +/- 1.5 min, respectively, after termination of the infusion. There was no cumulative effect after prolonged vecuronium infusion.