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Biomedical subjects

O Takamiya

Publications and source records attributed to O Takamiya.

At least 37 records · Page 2Linked to original sources
PubMed1993

[Factor VII for a molecular marker in hypercoagulable state].

We measured factor VII activity and antigen levels in plasma of pregnant women and patients with elevated serum FDP including patients with DIC who were supposed to be in hypercoagulable state, and compared the values with normal subjects. Both FVII activities measured by human placenta thromboplastin (hTF/FVIIc) and bovine brain thromboplastin (bTF/FVIIc) in normal plasma were correlated well with the FVII antigen levels (FVIIag). Measured hTF/FVIIc, bTF/FVIIc and FVIIag in pregnant women were 163 +/- 44%, 205 +/- 49% and 175 +/- 44% respectively, and each value had correlation. Thrombin-antithrombin III complex in these subjects was increased (7.85 +/- 2.25 mg/ml). However, antithrombin III, plasmin-plasmin inhibitor complex and FDP D-dimer were within normal range. These observations indicated that pregnant women were in hypercoagulable state but not in hyperfibrinolytic state. hTF/FVIIc, bTF/FVIIc and FVIIag in plasma from patients with elevated serum FDP were 59.6 +/- 29%, 94 +/- 65% and 61.6 +/- 26% respectively. We divided these patients into 2 groups: Group A (both prolonged PT and APTT) and Group B (shortened APTT). hTF/FVIIc, bTF/FVIIc and FVIIag in plasma of Group A were 47 +/- 21%, 48 +/- 24% and 48 +/- 21% respectively. The corresponding values of Group B were 80 +/- 24%, 155 +/- 54% and 74 +/- 23% which were correlated each other. Low levels of FVII observed in Group A seemed to be due to increased consumption of coagulation factors. In Group B, the pattern of FVII activities and FVII antigen was similar to that of pregnant women, though FVII levels were lower.(ABSTRACT TRUNCATED AT 250 WORDS)

Biomarkers↗
PubMed1992

A probable double heterozygous type II von Willebrand's disease with increased ristocetin induced platelet aggregation.

We have identified a patient with von Willebrand's disease (vWD) resembling type IIB vWD, with increased ristocetin induced platelet aggregation (RIPA), the absence of the large multimers of von Willebrand factor (vWF) in plasma, and the presence of the large multimers in platelets in whom a family study indicated a probable double heterozygous inheritance pattern. The propositus was a 12-year-old boy with frequent epistaxis and bruising. Abnormal hemostatic findings included a prolonged bleeding time (BT), decreased levels of factor VIII coagulant activity (VIIIC), von Willebrand factor antigen (vWF:Ag), ristocetin cofactor (RCof), and an increased RIPA. In the presence of ristocetin, binding of the patient's plasma vWF to normal platelets was increased but binding of normal vWF to his platelets was normal. SDS-agarose gel (1.5%) electrophoresis revealed that plasma vWF lacked the large multimers, and 3.0% gel electrophoresis revealed that the multimers had a 5-band pattern similar to normal. The above findings were consistent with type IIB vWD, but 1-deamino[8-D-arginine]-vasopressin (DDAVP) infusion resulted in a shortened BT and the transient appearance of large multimers without a decrease in the platelet count. Family studies revealed that his mother has mild bleeding symptoms, decreased VIIIC, vWF:Ag, and RCof levels and normal to slightly reduced RIPA with a multimer pattern consistent with type I vWD. In contrast, the father, sister, and paternal grandfather were asymptomatic, with a slightly decreased VIIIC level but a normal BT and vWF:Ag and RCof levels. Their RIPA and vWF binding to normal platelets were increased, but unlike the propositus their plasma contained large multimers. We concluded that the propositus is a type IIB-like variant differing from previously reported IIB variants in two ways: 1) his response to DDAVP and 2) a possible double heterozygous mode of inheritance rather than the usual dominant route.

Blood Platelets↗
PubMed1991

Fetal intracranial arterial hemodynamics assessed by color and pulsed Doppler ultrasound.

Fetal intracranial arterial velocimetries were serially performed on 31 pregnant women with regular menstrual cycle, at 28-40 weeks of gestation, using color and pulsed Doppler ultrasound. The change in fetal intracranial arterial hemodynamics was based on the calculation of resistance index (RI). There was no significant difference between the RI values in the anterior cerebral artery (ACA) and middle cerebral artery (MCA) from 28 weeks to term. The RI in the posterior cerebral artery (PCA) was significantly lower than that in the ACA and MCA, respectively, from 28 weeks to term (P less than 0.001). The PI values in both the ACA and MCA remained unchanged from 28 to 33 weeks, became significantly lower from 34 to 35 weeks (P less than 0.05), and the values were fairly constant thereafter. The RI seen in the PCA showed no significant change from 28 weeks to term. This color and pulsed Doppler ultrasound assessment of fetal intracranial arterial blood flow provides a useful foundation for elucidating normal intracranial hemodynamics and will aid in avoiding misinterpretations of the different intracranial arteries.

Blood Flow Velocity↗
PubMed1991

Congenital factor VII abnormality discovered in an infant at a routine checkup.

Congenital coagulation factor VII abnormality in an infant was first discovered because of a decreased normotest value at a routine health checkup at the age of 1 month. No bleeding tendency had been noticed. The normotest value did not respond to an administration of vitamin K. The prothrombin time (PT) was prolonged, and the activated partial thromboplastin time (A-PTT) was in the normal range. Factor VII activity was extremely low, whereas the level of factor VII antigen was relatively low. These data indicate that the propositus is a factor VII reduced variant, a rare variant of congenital factor VII deficiency. The propositus and her brother are homozygotes, and her parents and sister are heterozygotes.

Blood Coagulation Tests↗
PubMed1991

Transvaginal color and pulsed Doppler ultrasound evidence of hemodynamics of the female pelvis.

Transvaginal color and pulsed Doppler ultrasound examinations were made on 40 patients to assess hemodynamics in the pelvis. Use of a special probe for transvaginal scanning led to superb simultaneous visualization of structures and blood flow, and we noted various events, even in the small, deep and/or peripheral vessels. Some but not all of the observations were in agreement with the results obtained using transabdominal color and pulsed Doppler ultrasound. We propose that transvaginal color and pulsed Doppler ultrasound be considered for routine use to determine physiological and pathological events in the female pelvis.

Color↗
PubMed1991

[Assay of factor VII antigen by enzyme-linked immunosorbent assay (ELISA)].

This paper introduces a newly developed enzyme-linked immunosorbent assay (ELISA) method, using monospecific anti-human factor VII rabbit IgG, to quantify antigen levels of factor VII. The procedure is relatively simple, its method is remarkably specific and reproducible. Its sensitivity to detect factor VII antigen is as low as 0.015 U/ml, when factor VII antigen in 1 ml normal plasma is arbitrarily defined as 1 unit. The level of factor VII antigen in 4 patients with congenital factor VII deficiency and their family members was investigated through this ELISA and the results correlated well with those obtained by electroimmunoassay previously described. The significant correlation between the antigen levels and activity of factor VII was demonstrated in 4 patients with congenital factor VII deficiency and their family members, normal individuals and patients with liver disease. However, the level of factor VII antigen in patients treated with warfarin was higher than their coagulation activity.

Antigens↗
PubMed1990

Parametrial invasion of uterine cervical cancer assessed by transrectal ultrasonography: preliminary report.

Transrectal ultrasonographic examinations were performed on 30 patients with uterine cervical cancer to assess parametrial invasion. The findings were compared with data obtained by manual rectal examination. Forty-six parametria in 23 patients were histologically examined following surgery. Sensitivity, specificity, positive predictive value, negative predictive value, and predictive accuracy of transrectal ultrasonography and rectal examination were 1.0 and 0.25 (P less than 0.05), 0.9 and 0.93, 0.5 and 0.25, 1.0 and 0.93, and 0.91 and 0.87, respectively. Therefore, transrectal ultrasonography may prove to be a useful diagnostic tool to determine the preoperative staging of uterine cervical cancer.

Diagnostic Errors↗
PubMed1990

Change in ovarian arterial compliance during the human menstrual cycle assessed by Doppler ultrasound.

Ovarian arterial velocimetry, using real-time two-dimensional and pulsed Doppler ultrasound, was performed on 36 occasions in nine healthy women with regular menstrual cycles. The change in ovarian arterial compliance was based on the calculation of pulsatility index (PI). In the active ovary carrying a dominant follicle or corpus luteum, the PI in the early follicular phase (mean 6.97, SD 2.01) was significantly higher than that in the late follicular phase (mean 2.36, SD 0.31) (P less than 0.001), and the PI in the early luteal phase (mean 0.68, SD 0.09) was significantly lower than that in the late follicular phase (P less than 0.001). The PI in the late luteal phase (mean 0.93, SD 0.16) was significantly higher than that in the early luteal phase (P less than 0.01). In the inactive ovary, no cyclical changes were seen in the values of PI. There was a significant difference between the values of PI in the active ovary and the inactive ovary in the late follicular, the early and the late luteal phases [2.36 (SD 0.31)] vs [6.29 (SD 1.02)], [0.68 (SD 0.09)] vs [6.18 (SD 1.33)], [0.93 (SD 0.16) vs [6.57 (SD 1.72)] (P less than 0.001), respectively. Use of non-invasive Doppler ultrasound to study physiology of ovarian haemodynamics during the menstrual cycle is of great clinical relevance and paves the way for further investigations on sterility.

Adult↗
PubMed1990

Sonographic findings of uterine leiomyosarcoma.

We describe herein various sonographic features of uterine leiomyosarcoma. Transabdominal scanning (TAS) revealed an image indistinguishable from benign leiomyoma, with evidence of degeneration. Transvaginal scanning (TVS) clearly showed the thinness of the myometrium, and the possibility of deep myometrial invasion was suspected at intraoperative open direct ultrasonography (ODU). A very high peak systolic velocity and a slightly increased diastolic component at the periphery of the tumor were evident with pulsed Doppler ultrasound. Thus, TAS, TVS, ODU and Doppler ultrasound will yield useful information for the physician attempting to evaluate the extent and vascularity of uterine leiomyosarcoma.

Female↗
PubMed1990

Detection of intrauterine growth retardation based on measurements of size of the liver.

A total of 646 ultrasonic measurements of fetal liver size were made from 18 weeks of gestation through to term. After 29 weeks, size of the liver in the growth-retarded fetuses was significantly small as compared to findings in the control fetuses. The sensitivity and specificity for the detection of growth retardation were 0.86 and 0.89 at 33 weeks, 0.89 and 0.94 at 38 weeks and 0.89 at 40 weeks of gestation. Growth rate of the liver in the growth-retarded fetus was diminished as compared with findings in the control fetus. Also, a method of estimating fetal weight by ultrasonic measurement of the fetal liver size is described.

Birth Weight↗
PubMed1990

Efficiency of transvaginal scanning in obstetric and gynecologic fields.

Transvaginal ultrasonography was done on 26 pregnant women ranging from 5 to 27 weeks of gestation and 12 patients with gynecologic diseases. In early gestation, significant correlations were found among gestational age, crown-rump length, diameter of yolk sac and fetal heart rate. The maximal length of the gestational sac did not correlate with any other parameter. Transvaginal image quality was better in 76.2% in obstetric field and in 66.7% in gynecologic field, as compared to findings with conventional abdominal ultrasonography, respectively. Fine structures and individual organs were better evidenced transvaginally. Therefore, transvaginal ultrasound affords pertinent information complementary to that obtained with the transabdominal technique.

Female↗
PubMed1990

[An assay of lupus anticoagulants by use of plasma-agarose gel].

We developed a sensitive and quantitative method for assaying lupus anticoagulants. The method was based on the inhibition of fibrin formation in a plasma-agarose gel plate, which was described as a method for assaying hemophilic factor VIII-inhibitor (Bird, 1975). The final concentration of plasma in agarose gel was set up 30% instead of 50%. Fibrin formation was stopped, when the clear zone of normal plasma as negative control disappeared and that of 0.039 units heparin as positive appeared. Then this improved the precision of measurement. The method was not only more sensitive than the dilute KPTT of a 1:1 mixture with normal plasma, but had no false positive. The standard curve was linear at heparin units from 0.039 to 100. The assay value could be estimated with heparin titer. This method seem to be useful for quantitative assaying and for determining low titer in lupus anticoagulants.

Blood Coagulation Factors↗
PubMed1989

Umbilical artery velocity waveforms are not valid indices for assessing growth retardation in utero.

Blood flow velocity waveforms from the umbilical artery of 126 normal fetuses with no complications at 20-41 weeks and in 31 growth-retarded fetuses, were recorded using pulsed Doppler ultrasound. A total of 140 examinations were performed on normal fetuses and 49 on growth-retarded fetuses. The resistance index (RI) was used as an index of blood flow resistance. In 30 of the 31 growth-retarded fetuses (96.8%), the values of RI were within the mean +/- 2 S.D. of normal fetuses. Placental/fetal weight ratio (P/F ratio) was assessed. All values of P/F ratio of growth-retarded fetuses were within the mean +/- 2 S.D. of normal values. Small placentas expressed as a normal P/F ratio almost always associated with normal RI values. Therefore, measurement of RI on umbilical artery blood flow velocity waveform by Doppler ultrasound cannot serve as a valid diagnostic index to predict growth retardation of the fetus in utero.

Blood Flow Velocity↗
PubMed1989

Protein C in the neonatal period.

Protein-C activity and antigen were measured in 141 full-term infants during the first month of life. The levels of both protein-C activity and antigen were about one third the level for normal adults in cord blood, and significantly lower than the cord blood during the 1st to 2nd days of life. They increased with age progressively, but did not reach the lowest limit in normal adults even in the first month. The low ratio of protein-C activity and antigen was demonstrated in some infants within the first 4 days of life. The precipitin arc of neonatal infants, which had a discrepancy between protein-C activity and antigen levels, showed an anodal shift upon agarose gel electrophoresis in the presence of Ca2+. The abnormal protein C in the neonatal period may be regarded as protein induced by vitamin-K absence or antagonist.

Breast Feeding↗