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O Ditrich

Publications and source records attributed to O Ditrich.

60 records · Page 4Linked to original sources
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[The differentiation of Opisthorchis eggs by the ultrastructure of their outer membranes].

Using a TESLA-BS-300 scanning electron microscope, the ultrastructure of the outer membranes of oocysts of 8 opisthorchis species (Opisthorchis felineus, O. viverrini, O. geminus, Clonorchis sinesis, Amphimerus anatis, Metorchis albidus, Pseudamphistomum truncatum, Erchoviorchis lintoni) belonging to 4 subfamilies, as well as that of O. felineus oocysts of various geographical isolates (from the Ukraine, the Volga region, West Siberia, Kazakhstan) have been investigated. The nature of the ultrastructure of the membranes differs from species to species and can be used for the differentiation of opisthorchis oocysts. Marked differences have been observed in different opisthorchis subfamilies (Opisthorchinae, Metorchinae, Pseudamphistominae, Pseudamphimerinae). The membrane ultrastructure was not significantly different in O. felineus oocysts from various geographical isolates, however, there are some distinctions in the size of oocysts and the form of apical thorns and opercular endings. The work has been done within the framework of the international scientific cooperation between the E. I. Martsinovskiĭ Institute of Medical Parasitology and Tropical Medicine, USSR Ministry of Public Health and the Institute of Parasitology, the Czechoslovakian Academy of Sciences.

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[Immunoblotting in the study of the antigenic structure of Leishmania].

Using immunoblotting, the antigenic structure of 6 Leishmania strains has been studied: 1) MHOM/IN/80/DDS--Leishmania (Leishmania) donovani; 2) MHOM/SU/63/VL--L. sp. ZMA; 3) MHOM/SU/73/K-27--L. tropica; 4) MHOM/SU/73/5Ash--L. major; 5) MHOM/GE/84/H-132--L. mexicana amazonensis; 6) REPT/SU/83/3960-GC--L. (Sauroleishmania) gymnodactyli. Antigens of Leishmania surface membranes and rabbit antisera against them have been used. Among the agents of leishmaniasis in the Old World (1-4), the most intensive antigenic lines were found in the medium and high-molecular mass area (43-200 kD). In L. (L) mexican a amazonensis (5) species-specific lines have been identified in 10-22 kD area, which is indicative of considerable antigenic differences in Leishmania of the Old and New Worlds. The most marked antigenic differences from other types were noted in L. (S) gymnodactyli (6). It was characterized by the absence of antigenic bands in the medium and high molecular mass areas, the lines associated with species-specific determinants located in the low molecular mass area (18 and 25 kD). The results of cross-reacting c-ELISA using the same antigens and antisera correlated well with the above data of immunoblotting. Immunoblotting may be used for identification of species-specific antigenic structures which may be helpful in serological tests for more accurate leishmania identification and leishmaniasis diagnosis.

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